Journal of translational science最新文献

{"title":"Hyperglycemia induces osteogenic differentiation of tendon derived mesenchymal stem cells","authors":"A. GiaiVia, M. Mccarthy, M. Francke, G. Pipino, F. Oliva, A. Mazzocca, N. Maffulli","doi":"10.15761/jts.1000417","DOIUrl":"https://doi.org/10.15761/jts.1000417","url":null,"abstract":"Introduction: The physiopathology of calcific tendinopathy is largely unknown, but it could be the result of an active cell-mediated process. Many endocrine and metabolic diseases may impair the homeostasis of the tendon, and recent studies focus on the influence of extracellular matrix on the differentiation pathway of mesenchymal stem cells (MSCs). The present study investigates whether hyperglycemia may influence the differentiation of tendon derived-MSCs (TD-MSCs) into an osteoblast lineage. Methods: MSCs were harvested from discarded human tendon excised during arthroscopic rotator cuff repair. The resulting TD-MSCs were counted, plated and grown to confluence in supplemented medium ( α MEM). TD-MSCs were treated with α MEM containing low (5.0 mM), physiological (10 mM) or high (25 mM) glucose, (+) and (-) 10 -10 mM insulin for 0, and 24 hours, 7, 14 and 30 days. Control cells were treated with α MEM. Only quantitative polymerase chain reaction (qPCR) was used to measure changes in gene expression levels specific for fibrocartilage of TD-MSCs (collagen type I-II-III, aggrecan, osteopontin, fibronectin and alkaline phosphatase). Calcium levels were measured after 30 days in culture. Immunohistochemistry staining was used to determine the amount of the specific proteins present in each group tested. Results: There was an increased gene expression of collagen type I, alkaline phosphatase, aggrecan and osteopontin in TD-MSCs supplemented with high glucose compared to other groups (p<0.05). When insulin was added to the α MEM, a higher increase of collagen type I and III was found in TD-MSCs cultured with high dose glucose. The synthesis of alkaline phosphatase and the expression of aggrecan and osteopontin genes were higher in TD-MSCs cultured with high dose glucose and high dose glucose with insulin. After 30 days, calcium content was increased in the high glucose group and in the high glucose medium and insulin. Conclusions: When cultured in a high glucose medium, TD-MSCs express bone markers, and are able to differentiate toward an osteoblast lineage. These results reinforce the concept that calcific tendinopathy may be caused by erroneous differentiation of MSCs in the presence of high levels of serum glucose.","PeriodicalId":74000,"journal":{"name":"Journal of translational science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67492371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial effects of cellulose derivatives in the prevention of surgical infections: in vitro activity against-resistant microorganisms","authors":"H. Khorsi-Cauet, M. Barry, G. Touati, Hamdad Farida, V. Bach, M. Biendo","doi":"10.15761/jts.1000422","DOIUrl":"https://doi.org/10.15761/jts.1000422","url":null,"abstract":"","PeriodicalId":74000,"journal":{"name":"Journal of translational science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67492700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dysregulated circadian clock and aged stem cell: An inflammatory crosstalk?","authors":"Dan Li, Yuting Tan","doi":"10.15761/jts.1000438","DOIUrl":"https://doi.org/10.15761/jts.1000438","url":null,"abstract":"Aging occurs in response to many triggers, including cellular senescence, telomere attrition, DNA damage, organelle stress, oncogene activation and stem cell dysfunction [1]. Among these, the aged stem cell per sec can exhibit various aging hallmarks, such as increased DNA damage, dysregulated mitochondrial function, and less efficient protein recycling. Aged stem cell is a prime factor that undermines organismal homeostasis, which can facilitate numerous pathologies including cancer, neuropathy, sarcopenia and immunopathy [2].","PeriodicalId":74000,"journal":{"name":"Journal of translational science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67493369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic hallmarks of cancer cells as targets for integrative therapies","authors":"J. Ionescu, A. Constantinescu","doi":"10.15761/JTS.1000390","DOIUrl":"https://doi.org/10.15761/JTS.1000390","url":null,"abstract":"","PeriodicalId":74000,"journal":{"name":"Journal of translational science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67491268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GMP-grade allogeneic musculoskeletal primary progenitor cell types: Standardized candidates for general or pharmacopeial monograph elaboration","authors":"A. Laurent, Jeanne-Pascale Simon, N. Hirt-Burri, W. Raffoul, L. Applegate, A. D. B. Roessingh","doi":"10.15761/jts.1000406","DOIUrl":"https://doi.org/10.15761/jts.1000406","url":null,"abstract":"1Regenerative Therapy Unit, Lausanne University Hospital, University of Lausanne, CH-1066 Epalinges, Switzerland 2Plastic, Reconstructive and Hand Surgery Service, Lausanne University Hospital, University of Lausanne, CH-1011 Lausanne, Switzerland 3Unit of Legal Affairs, Lausanne University Hospital, University of Lausanne, CH-1011 Lausanne, Switzerland 4Competence Center for Applied Biotechnology and Molecular Medicine, University of Zurich, CH-8057 Zurich, Switzerland 5Oxford Suzhou Center for Advanced Research, Science and Technology Co. Ltd., Oxford University, Suzhou 215000, PRC 6Pediatric Surgery Service, Lausanne University Hospital, University of Lausanne, CH-1011 Lausanne, Switzerland","PeriodicalId":74000,"journal":{"name":"Journal of translational science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67492071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Telemetric hemodynamic assessment during anesthesia induction with propofol in experimental heart failure","authors":"Julia Vogel, P. Boehme, R. Wakili, Katharina Boden, T. Reimer, P. Sandner, Jörg Hüser, W. Dinh, H. Truebel, T. Mondritzki","doi":"10.15761/jts.1000408","DOIUrl":"https://doi.org/10.15761/jts.1000408","url":null,"abstract":"aminobutyric with reduced ventricular diastolic ventricular Millimeters Magnetic tomography; blood Standard natriuretic Abstract Background: The influence of initial propofol on cardiac hemodynamics in patients suffering from heart failure (HF) is insufficiently understood and contradicting opinions are existing. Nevertheless, propofol is often used in HF patients if etomidate is contraindicated. The development of novel telemetry sensors allows the assessment of intracardiac pressure signals in conscious animals and thus the investigation of anesthesia induction. Here, we investigated the hemodynamic effects of propofol in healthy dogs in comparison to dogs affected with HF. Methods: Telemetry sensors and pacemaker were implanted in six male beagle dogs. After wound healing, the pacemaker was programmed to 220 bpm and later to 180 bpm to induce and maintain heart failure. At baseline conditions (before pacing) and at several time points (Day 14, 28 and 42) during HF progression all animals were anesthetized with 5 mg/kg propofol i.v. Hemodynamic measurements were done to assess the effects of propofol during anesthesia induction Results: After induction with propofol, we observed a significant (p<0.001) increase in heart rate (HR) for all groups (healthy: +63 ± 53 bpm, 14d: +48 ± 23 bpm, 28d: +45 ± 20 bpm, 42d: +51 ± 28 bpm in peak) compared to individual baseline values. In addition, blood pressure (BP) increased in all HF groups (in average +17.4 ± 1.4 mmHg, p<0.05), while this effect was not present under healthy conditions. We further observed a trend towards an increase in left ventricular contractility (+dP/ dtmax) by +621.1 ± 157.0 mmHg/s (averaged peak data) in all groups. Conclusions: In dogs affected with heart failure, initial anesthesia with propofol resulted in an increase in heart rate and blood pressure, whereas no cardio depressive adverse effects could be observed on HF-relevant parameters. Therefore, propofol might be an alternative for short term anesthesia in HF patients. Nevertheless, further animal and human studies are needed to evaluate if these data can be translated into the clinical setting.","PeriodicalId":74000,"journal":{"name":"Journal of translational science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67492140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virtual reality hypnosis improves sleep quality of first-line medical staff responding to COVID-19","authors":"Guo Zhongwei, Duan Xin, W. Lijuan, Zheng Jisheng, Lu Longxi","doi":"10.15761/jts.1000410","DOIUrl":"https://doi.org/10.15761/jts.1000410","url":null,"abstract":"","PeriodicalId":74000,"journal":{"name":"Journal of translational science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67492388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LVAD driveline infection: Pump cable relocation in two stage surgical strategy and preventive partial driveline replacement","authors":"J. Zeitani, S. Kuçi, A. Ibrahimi, Antonio Pellegrino, E. Likaj","doi":"10.15761/jts.1000442","DOIUrl":"https://doi.org/10.15761/jts.1000442","url":null,"abstract":"","PeriodicalId":74000,"journal":{"name":"Journal of translational science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67494178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Late CardioMEMS migration: Coughing spells as a cause of concern","authors":"P. Codina, O. Altisent, Ignasi Guasch-Arriaga, E. Santiago‐Vacas, J. Lupón, A. Bayés‐Genís","doi":"10.15761/jts.1000443","DOIUrl":"https://doi.org/10.15761/jts.1000443","url":null,"abstract":"","PeriodicalId":74000,"journal":{"name":"Journal of translational science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67495398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aberrant left hepatic artery from the gastroduodenal artery with no cystic branch: Case report and review of literature","authors":"Bortolussi Carlo, Portale Teresa Rosanna, Costa Salvatore Domenico, Meli Gaetano Alfio, Allocco Roberto, Di Stefano Biagio","doi":"10.15761/jts.1000420","DOIUrl":"https://doi.org/10.15761/jts.1000420","url":null,"abstract":"Anatomical variants of the hepatic arteries are often leaved out in anatomical atlases and university corse, even if represented in on the of gallbladder left cystic","PeriodicalId":74000,"journal":{"name":"Journal of translational science","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67493016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}