Tanveer Ahmad , Mirza Abrar , Syeda Anum Masood Bokhari , Shahid Iqbal , Abid Mahmood Alvi , Qumer Iqbal , Muhammad Awais Ghani , Muhammad Umar , Raja Tahir Iqbal
{"title":"Amaranthus: A nutritious solution to malnutrition for underprivileged populations in Pakistan","authors":"Tanveer Ahmad , Mirza Abrar , Syeda Anum Masood Bokhari , Shahid Iqbal , Abid Mahmood Alvi , Qumer Iqbal , Muhammad Awais Ghani , Muhammad Umar , Raja Tahir Iqbal","doi":"10.1016/j.jtemin.2025.100221","DOIUrl":"10.1016/j.jtemin.2025.100221","url":null,"abstract":"<div><div>Malnutrition in Pakistan is cause of concern which is affecting everyone regardless of age and gender. The situation for women and children is even more alarming and prevalence of malnutrition in Pakistan exceeds against other developing countries. The poor and underprivileged populations in Sindh, Balochistan, KPK and South Punjab are severely affected by undernutrition of macro and micronutrients. Despite continuous efforts, malnutrition in certain areas of Pakistan is on surge due to many factors. Poverty, lack of sufficient nutritious food coupled with lack of food diversity are main factors of undernutrition in Pakistan. Amaranth is a climate resilient; drought tolerant plant and it can be produced with minimal number of resources even in poor soils. The adaptability and nutritional value of 25 exotic vegetable amaranth genotypes were investigated using standard analytical approaches in order to evaluate the several possibilities of the plant leaves in underprivileged population of Pakistan. The study discovered that exotic amaranth genotypes produce abundant green matter with optimum moisture contents and number of leaves per plant with good height. The investigation for nutrients disclosed the maximum ash content, crude protein, crude fat, crude fiber and carbohydrate from the leaves as (4.32 g 100<em>g</em><sup>−1</sup> FW), (6.76 g 100<em>g</em><sup>−1</sup> FW), (0.66 g 100<em>g</em><sup>−1</sup> FW), (4.29 g 100<em>g</em><sup>−1</sup> FW) and (18.19 g 100<em>g</em><sup>−1</sup> FW) respectively in different genotypes. The maximum energy yielded in calories was 407.41 (kJ 100<em>g</em><sup>−1</sup> FW). Elemental breakdown was performed in (mg g<sup>−1</sup> DW) and it indicated that the leaves contained maximum potassium (15.52 mg g<sup>−1</sup> DW), calcium (11.11 mg g<sup>−1</sup> DW), Magnesium (9.67 mg g<sup>−1</sup> DW), Iron (0.24 mg g<sup>−1</sup> DW), Zinc (0.18 mg g<sup>−1</sup> DW) and phosphorus (5.85 mg g<sup>−1</sup> DW) in different genotypes under investigation. Comparing the nutrients in exotic vegetable amaranth genotypes with recommended dietary allowance (RDA) values, the outcomes expose that the leaves of amaranth contain substantial amount of important nutrients that are crucial in combating malnutrition in underprivileged populations in Pakistan.</div></div>","PeriodicalId":73997,"journal":{"name":"Journal of trace elements and minerals","volume":"12 ","pages":"Article 100221"},"PeriodicalIF":0.0,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Istiak Ahmed , Md. Mostafa Monwar , Md Iqram Uddin Al Amran , Md Mehedi Iqbal , Israt Sultana Isha , Md Masum Billah , Mohammad Rafiqul Islam , Abdullah Al Mamun , Md Khurshid Alam Bhuiyan
{"title":"Are industrial footprints driving potentially toxic element contamination in the Karnaphuli River Estuary?","authors":"Istiak Ahmed , Md. Mostafa Monwar , Md Iqram Uddin Al Amran , Md Mehedi Iqbal , Israt Sultana Isha , Md Masum Billah , Mohammad Rafiqul Islam , Abdullah Al Mamun , Md Khurshid Alam Bhuiyan","doi":"10.1016/j.jtemin.2025.100220","DOIUrl":"10.1016/j.jtemin.2025.100220","url":null,"abstract":"<div><h3>Background</h3><div>The contamination of estuarine sediments with potentially toxic elements (PTEs) is a growing concern in industrialized regions. The Karnaphuli River estuary, a critical ecosystem in Bangladesh, is exposed to untreated industrial, agricultural, and urban effluents, posing ecological and health risks.</div></div><div><h3>Objective</h3><div>This study aimed to assess the levels and spatial distribution of chromium (Cr), cadmium (Cd), lead (Pb), copper (Cu), zinc (Zn), and nickel (Ni) in sediments of the estuary, along with potential contamination sources and the associated ecological risks.</div></div><div><h3>Methods</h3><div>Sediment samples were collected from six key discharge points and analyzed using atomic absorption spectrophotometry. The data were examined to identify contamination patterns using geostatistical techniques, element correlations, and possible pollution sources. Results were compared with international sediment quality guidelines and findings from global estuarine systems to assess ecological risks.</div></div><div><h3>Results</h3><div>The concentrations of Cr (77.13–165.29 mg kg<sup>-1</sup>), Zn (67.86–83.29 mg kg<sup>-1</sup>), and Cu (53.28–73.66 mg kg<sup>-1</sup>) were highest near industrial zones. Strong correlations were observed among Cr, Zn, and Pb, indicating overlapping anthropogenic sources such as industrial discharges and urban runoff. When compared to global estuarine systems, the contamination levels of PTEs in the Karnaphuli estuary were found to be moderate to high, reflecting similar patterns observed in other industrialized regions. Several PTEs exceeded permissible limits, raising significant ecological concerns.</div></div><div><h3>Conclusions</h3><div>The findings underscore the need for stricter wastewater management, advanced remediation technologies, and robust monitoring frameworks to mitigate contamination risks. The study provides a baseline for policymaking to protect estuarine ecosystems in rapidly industrializing regions.</div></div>","PeriodicalId":73997,"journal":{"name":"Journal of trace elements and minerals","volume":"12 ","pages":"Article 100220"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hiwa Mohammad Qadr, Midya Hussen Ibrahim, Hanifa Younis Hassan
{"title":"Determination of potentially toxic heavy metals in milk powder marketed in Kurdistan of Iraq","authors":"Hiwa Mohammad Qadr, Midya Hussen Ibrahim, Hanifa Younis Hassan","doi":"10.1016/j.jtemin.2025.100219","DOIUrl":"10.1016/j.jtemin.2025.100219","url":null,"abstract":"<div><h3>Background</h3><div>Milk products have become a popular human nutrient in recent years because of their vitamins and minerals that are essential for the development and function of various organs and tissues. Additionally, excessive consumption of these vitamins and minerals may adversely affect our health.</div></div><div><h3>Objective</h3><div>This study aimed to determine the concentration of five potentially toxic heavy metals, namely cadmium (Cd), lead (Pb), nickel (Ni), copper (Cu), and zinc (Zn) in 14 samples of powdered milk collected from the Iraqi Kurdistan Region markets. Furthermore, the experimental data were then used to determine the potential health risk for an adult age through the metal pollution index (MPI), estimated daily intake of metals (EDIM), human health risk index (HHRI) and hazard index (HI)</div></div><div><h3>Methodology</h3><div>In this study, the concentrations were determined using energy dispersive X-ray fluorescence (EDXRF).</div></div><div><h3>Results</h3><div>The findings demonstrate that the mean concentrations of Cd, Pb, Ni, Cu, and Zn in powdered milk samples were 0.079, 0.042, 0.077, 0.056 and 0.847 mg kg<sup>−1</sup>, respectively. Human health was found to be very lowly affected by these metals based on the results of the estimated daily intake of metals (EDIM). Hazard index (HI) values in 36 % were less than 1 and 64 % were higher than 1.</div></div><div><h3>Conclusion</h3><div>The consumption of powdered milk by adults may pose potential health risks, and other protein sources should be monitored for metals.</div></div>","PeriodicalId":73997,"journal":{"name":"Journal of trace elements and minerals","volume":"12 ","pages":"Article 100219"},"PeriodicalIF":0.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Source apportionment, ecological and toxicological risk assessment of trace metals in agricultural soils of Wabane, South West Region, Cameroon","authors":"Guedjeo Christian Suh , Ngambu Aloysius Afahnwie , Anicet Feudjio Tiabou , Kouankap Nono Gus Djibril , Adze Rene Meniemoh , Lemnyuy Prosper Yiika","doi":"10.1016/j.jtemin.2025.100218","DOIUrl":"10.1016/j.jtemin.2025.100218","url":null,"abstract":"<div><h3>Background</h3><div>This study seeks to evaluate the origins, ecological implications, and ecotoxicological risks of trace metals using single and cumulative pollution indicators as well as mitigation strategies in the agricultural soils of Wabane, Cameroon.</div></div><div><h3>Methods</h3><div>The soil samples were analyzed by ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometry).</div></div><div><h3>Results and Discussion</h3><div>The agricultural soil samples exhibit high levels of Al<sub>2</sub>O<sub>3</sub> and Fe<sub>2</sub>O<sub>3</sub>, which have high adsorption capacity for trace metals and are important carriers of trace metal migration and enrichment. The results highlight significant concentrations of Co, Cu, Cr, Ni, Hg, Mn, La, Sn and Zn above the geochemical threshold values, sourced from mixed provenance. Contamination assessment indicators: contamination factor (CF: 1–6), pollution load index (PLI: 0.02–1.23), and enrichment factor (EF: 0.21–604.80) suggest soil contamination and metallic pollution. The potential ecological risk indicators show minimal ecological effect of trace metals on the soil ecosystem. Toxic risk index (TRI: 0.36–7.60) and toxicity unit analysis (TUs: 0.004 to 0.96) indicates low toxicity, suggesting low toxicological effects of trace metals on the soil ecosystem. According to Pearson correlation matrix and principal component analysis, while Co, Cr, Cu, Ni, Zn and V were obtained from geogenic provenance, As, Cd, Pb, Hg and Mn originate from geogenic as well as anthropogenic input sources.</div></div><div><h3>Conclusion</h3><div>Lithogenic processes and anthropogenic practices contribute to contamination and metallic pollution of the soil ecosystem.</div></div><div><h3>Recommendations</h3><div>Concrete monitoring and mitigation measures (low metal cultivars, biochar, etc.) must be taken to reduce trace metal accumulation from point and non-point sources entering agricultural soils. This research acts as a baseline study to potential soil contamination and metal toxicity in soils within the vicinity of Mount Bamboutos.</div></div>","PeriodicalId":73997,"journal":{"name":"Journal of trace elements and minerals","volume":"12 ","pages":"Article 100218"},"PeriodicalIF":0.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María Victoria Panzl , Jarol R. Miranda-Andrades , Wendy J. Sandoval Rojano , Joseane A. Mendes , Tatiana D. Saint'Pierre , Alejandra Rodríguez-Haralambides , Ricardo Q. Aucelio
{"title":"Trace element and mercury speciation analysis in yerba mate (Ilex paraguariensis)","authors":"María Victoria Panzl , Jarol R. Miranda-Andrades , Wendy J. Sandoval Rojano , Joseane A. Mendes , Tatiana D. Saint'Pierre , Alejandra Rodríguez-Haralambides , Ricardo Q. Aucelio","doi":"10.1016/j.jtemin.2025.100217","DOIUrl":"10.1016/j.jtemin.2025.100217","url":null,"abstract":"<div><h3>Background</h3><div>Yerba mate, a popular beverage in South America, is rich in bioactive compounds and minerals. However, yerba mate leaves may contain toxic metals and environmental contaminants. This study performed multi-element analyses using ICP-MS and ICP OES and mercury speciation by GC-CV-AFS and CV–AAS to profile infusions of ten yerba mate samples consumed in Brazil and Uruguay.</div></div><div><h3>Materials and methods</h3><div>For ICP OES and ICP-MS analyses, 500 mg infusions of samples were prepared and diluted with 10 % v/v HNO<sub>3</sub>. Ultrasonic-assisted extractions of mercurial species were made followed by distillation and dilution with water for CV-AAS. For GC-CV-AFS speciation, samples were extracted and distilled, using 200 μL aliquots for analysis.</div></div><div><h3>Results</h3><div>The most abundant elements in the digested solid samples were K, Ca, and Mg, whereas in infusions the ranges were up to 9.18 mg g<sup>−1</sup> for K, 0.68 mg g<sup>−1</sup> for Ca, and 2.74 mg g<sup>−1</sup> for Mg. Pb presented a mean concentration of 260 µg kg<sup>−1</sup> in leaves but it was not found in infusions. Hg<sup>2+</sup> was found from 0.67 to 0.96 μg kg<sup>−1</sup> in leaves with one sample presenting 0.67 μg kg<sup>−1</sup> of CH<sub>3</sub>Hg.</div></div><div><h3>Conclusion</h3><div>K, Ca, and Mg were the most abundant elements in the yerba mate samples. Four samples showed ultra-trace concentrations of Hg<sup>2+</sup> and one of CH<sub>3</sub>Hg.</div></div>","PeriodicalId":73997,"journal":{"name":"Journal of trace elements and minerals","volume":"12 ","pages":"Article 100217"},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143369855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leilane R. Santos , Filipe B. Santana , Hilária F.A. Silveira , Wellington C. de Carvalho , Laís A. Souza , Rennan Geovanny O. Araujo , Maria Graças A. Korn , Daniele Cristina M.B. Santos
{"title":"Inorganic constituents in cassava derivatives: Total concentration, dietary intake and bioaccessibility","authors":"Leilane R. Santos , Filipe B. Santana , Hilária F.A. Silveira , Wellington C. de Carvalho , Laís A. Souza , Rennan Geovanny O. Araujo , Maria Graças A. Korn , Daniele Cristina M.B. Santos","doi":"10.1016/j.jtemin.2025.100216","DOIUrl":"10.1016/j.jtemin.2025.100216","url":null,"abstract":"<div><h3>Introduction</h3><div>The objective of this study was to evaluate the concentration and in vitro bioaccessibility of inorganic constituents in cassava (Manihot esculenta Crantz) derivatives marketed in Brazil, aiming to uncover new data on the distribution and bioaccessibility of Mn and Mg. By identifying patterns in bioaccessibility, the study aims to provide crucial insights into the dietary impact of these chemical elements, with a focus on nutritional benefits and health risks.</div></div><div><h3>Methods</h3><div>The concentrations of As, Ba, Cd, Ca, Co, Cu, Fe, K, Mg, Mn, Na, Pb, Se, Sr, V, and Zn were determined in cassava samples using inductively coupled plasma optical emission spectrometry and inductively coupled plasma mass spectrometry after sample decomposition employed closed digester block and diluited nitric acid.</div></div><div><h3>Results and Discussion</h3><div>The concentration ranges (in μg g<sup>-1</sup>) were: Ba (<0.32 - 0.60), Ca (<11- 542), Fe (<0.39 - 111), K (156 - 7362), Mg (9.8 - 533), Na (<3.1 - 98), P (58 - 1774) and Sr (<0.005 - 3.85). The concentration ranges (in ng g<sup>-1</sup>) obtained were: As (<12 - 158), Cd (<9 - 47), Co (<17 - 295), Cu (<90 - 1159), Mn (70 - 2523), Pb (<14 - 521), Se (<16 - 215), V (<6 - 106) and Zn (<3208 - 7277). The consumption of 100 g of starch contributes to the intake of Ca (1.0 - 2.8 %), Cu (3.8 - 2.9 %), Fe (1.2 - 12.4 %), Mg (0.2 – 11.0 %), Mn (0.6 - 8.0 %), P (1.6 - 18.0 %), Se (26.3 – 26.8 %) and Zn (4.0 – 6.0 %). No health risk was distributed for As and Cd. For Pb, the concentrations were three times higher than the legislated values in Brazil. The estimated bioaccessibility of Mg (up to 80 %) and Mn (up to 43 %) suggests that these chemical elements can be available for absorption and may contribute positively to dietary intake when consuming cassava derivatives.</div></div><div><h3>Conclusion</h3><div>This study makes a significant contribution by proposing a simple and low-cost procedure for evaluating the mineral composition of cassava samples, as well as bioaccessibility estimation of Mn and Mg, providing new nutritional information of interest to public health.</div></div>","PeriodicalId":73997,"journal":{"name":"Journal of trace elements and minerals","volume":"12 ","pages":"Article 100216"},"PeriodicalIF":0.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laxman S. Vijapur , Mallappa Shalavadi , Anita R. Desai , Jayadev N. Hiremath , Avinash S. Gudigennavar , Somlingesh L. Shidramshettar , Shreyanka R. Hiremath , Malleswara Rao Peram , Bharatesh S. Kittur
{"title":"Wound healing potential of green synthesized silver nanoparticles of Glycyrrhiza glabra linn root extract: A preclinical study","authors":"Laxman S. Vijapur , Mallappa Shalavadi , Anita R. Desai , Jayadev N. Hiremath , Avinash S. Gudigennavar , Somlingesh L. Shidramshettar , Shreyanka R. Hiremath , Malleswara Rao Peram , Bharatesh S. Kittur","doi":"10.1016/j.jtemin.2025.100214","DOIUrl":"10.1016/j.jtemin.2025.100214","url":null,"abstract":"<div><h3>Background</h3><div>The research emphasizes the eco-friendly and sustainable approach of nanoparticle synthesis, leveraging the medicinal properties inherent in <em>Glycyrrhiza glabra</em> extract, which is known for its antimicrobial, anti-inflammatory, and antioxidant properties. By combining the therapeutic potential of the plant extract with the wound-healing capabilities of silver nanoparticles, the study seeks to develop an innovative, biocompatible, and effective treatment for enhanced wound healing process in excision and incision rat model.</div></div><div><h3>Methods</h3><div><em>Glycyrrhiza glabra</em> Linn root was extracted with alcohol and utilized in the green synthesis of silver nanoparticles. The green synthesized nanoparticles were characterized, formulated into gel, and evaluated for their potential to heal wound in rat wound model.</div></div><div><h3>Results</h3><div>The results of the study showed that the detection of ultra-violet visible spectroscopy peaks at 412 nm and 403 nm. The presence of tannins, saponins, flavonoids, and phenols in plant extract caused the capping and bio-reduction of silver, as demonstrated by FTIR. Silver nanoparticles that were biosynthesized were in between 69.7 and 419.1 nm in size. The study's formulation of synthesized silver nanoparticles demonstrated significant wound healing potential by promoting wound contraction and accelerating healing after 21 days of study, which was demonstrated by histopathological evaluation and complete epithelialization with regenerated hair follicles and higher collagen tissue.</div></div><div><h3>Conclusion</h3><div>The current study found that the biomolecules of <em>Glycyrrhiza glabra</em> Linn root alcoholic extract, which are responsible for augmenting the plant extract's capacity to heal wounds, were capped on silver nanoparticles.</div></div>","PeriodicalId":73997,"journal":{"name":"Journal of trace elements and minerals","volume":"11 ","pages":"Article 100214"},"PeriodicalIF":0.0,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of various feed additives on nutrient digestibility, digestive enzyme activity, blood metabolite levels, and gut microbiota composition in japanese quail chicks","authors":"Hassan Saleh , Omid Jangjou , Mohammad Taher Mirakzehi , Seyedeh Hamideh Hosseni","doi":"10.1016/j.jtemin.2025.100213","DOIUrl":"10.1016/j.jtemin.2025.100213","url":null,"abstract":"<div><h3>Background</h3><div>The poultry industry is currently facing significant challenges in maintaining disease control and achieving optimal growth following the removal of growth-promoting antibiotics. The objective of the current study was to evaluate the N Y effects of fumaric acid (FUA), iron oxide nanoparticles (IONPs), probiotics (Protaxin), and prebiotics (active MOS, mannan oligosaccharide) on nutrient digestibility, digestive enzymes, blood metabolites, antioxidant and immune status, and intestinal microbiota in Japanese quail chicks.</div></div><div><h3>Methods</h3><div>A total of 600 hatched Japanese quail (1-week-old) were randomly allocated to six groups, each containing five replicates with 20 birds in each. The birds were fed a diet based on corn and soybeans (negative control, NC) and supplemented with 0.25 g/kg of tetracycline antibiotic (PC), 0.20 g/kg of FUA, 0.4 g/kg of IONPs, 0.1 g/kg of probiotic (PRO), or 0.4 g/kg of prebiotic (MOS).</div></div><div><h3>Results</h3><div>Japanese quail chicks that were fed a diet containing iron oxide nanoparticles and antibiotics exhibited a statistically significant increase (<em>p</em> < 0.05) in body weight gain (BWG) between 1 and 5 weeks of age (5.65 and 5.67 g/d, respectively). Among the experimental groups, those that received diets supplemented with iron oxide nanoparticles, fumaric acid, and antibiotics demonstrated the most favorable feed conversion coefficient during the 1–5-week period. Quails that were fed fumaric acid and probiotics exhibited the highest levels of digestibility for crude protein (CP) and crude fiber (CF), respectively. Quails that were administered fumaric acid displayed the highest levels of amylase and protease activity compared to the other groups (17.75 and 0.84 U/I, respectively). However, no significant difference in lipase activity was observed among the various dietary supplements. The levels of LDL, TG, MDA, ALT, IgG, and IgM were influenced by the experimental treatments. Quail chicks fed diets containing iron oxide nanoparticles and fumaric acid displayed a higher prevalence of <em>Lactobacillus</em> and <em>Bacillus</em> species. In contrast, quail chicks that received diets containing tetracycline antibiotics exhibited a decreased presence of <em>Clostridium</em> species and <em>E. coli</em> in the ileum, as compared to the control group (<em>p</em> < 0.05).</div></div><div><h3>Conclusion</h3><div>The inclusion of iron oxide nanoparticles and fumaric acid supplementation in the diet of quail chicks has been found to enhance their growth, nutrient digestibility, immune response, antioxidant status, digestive enzymes, and intestinal health. Furthermore, these additives demonstrate potential as alternatives to antibiotics in the diet of in Japanese quail chicks.</div></div>","PeriodicalId":73997,"journal":{"name":"Journal of trace elements and minerals","volume":"11 ","pages":"Article 100213"},"PeriodicalIF":0.0,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Obinna O. Uchewa, Chinedu B. Egwuagu, Ogugua A. EGWU, Augustine O. Ibegbu
{"title":"Clove oil as a neuromodulator in environmental cadmium cognitive impairment on the prefrontal cortex of Wistar rats","authors":"Obinna O. Uchewa, Chinedu B. Egwuagu, Ogugua A. EGWU, Augustine O. Ibegbu","doi":"10.1016/j.jtemin.2025.100212","DOIUrl":"10.1016/j.jtemin.2025.100212","url":null,"abstract":"<div><h3>Background</h3><div>Trace elements in the environment are neurotoxins and severely affect the nervous system, which can impair cognitive ability.</div></div><div><h3>Objective</h3><div>To investigate the neuromodulatory role of clove oil (CLO) in Cd-induced cognitive impairment.</div></div><div><h3>Methods</h3><div>Thirty-five Wistar rats randomly assigned into five groups of seven were used. Group A received water, group B received 20 mg/kg of Cd, C received 10 mg/kg of CLO, D received 20 mg/kg of CLO, and E received 20 mg/kg of DMSO. All groups except A received 20 mg/kg of Cd before treatment. Sociability Chamber Tests (SCT) and Novel Object Recognition Tests (NORT) were used to test recognition memory as social behavior in rodents. The estimated GST, GPx, GSH, and GOT enzymes were by blood sample.</div></div><div><h3>Results</h3><div>GSH and GST levels significantly decreased in the untreated but increased in the treated groups at <em>P</em> < 0.05. The discrimination index reduced in the untreated group (<em>p</em> < 0.05) while the reduction continued in groups C and E (0.14±0.09 and 0.27±0.02), and group D increased (0.81±0.02). In the SCT, group B spent significant time in the common chamber, which increased significantly in groups C and D but decreased in E. Microscopically, group A showed normal histoarchitecture and several alterations in group B. In contrast, treated groups showed improvement in cell regeneration.</div></div><div><h3>Conclusion</h3><div>Clove oil alleviates cadmium toxicity by increasing antioxidant activity and improving cognition in rats.</div></div>","PeriodicalId":73997,"journal":{"name":"Journal of trace elements and minerals","volume":"11 ","pages":"Article 100212"},"PeriodicalIF":0.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sima Sarlak , Sayed Ali Tabeidian , Majid Toghyani , Amir Davar Foroozandeh Shahraki , Mohammad Goli , Mahmood Habibian
{"title":"Organic iron and iodine in laying hen diet: Performance, egg quality, thyroid hormones, antioxidant status, serum and yolk lipids, and egg iron and iodine contents","authors":"Sima Sarlak , Sayed Ali Tabeidian , Majid Toghyani , Amir Davar Foroozandeh Shahraki , Mohammad Goli , Mahmood Habibian","doi":"10.1016/j.jtemin.2025.100211","DOIUrl":"10.1016/j.jtemin.2025.100211","url":null,"abstract":"<div><h3>Introduction</h3><div>Eggs can be enriched with iron and iodine through the diet. However, high levels of iron and iodine negatively influence the egg quality and performance of birds. This problem may be overcome by organic forms of minerals.</div></div><div><h3>Materials and methods</h3><div>In this study, the effects of ferrous glycine and ethylenediamine dihydroiodide as sources of iron and iodine on hen performance, egg quality, antioxidant status, and iron and iodine contents of eggs were evaluated in a 16-wk dietary experiment. A factorial arrangement including 3 levels (0, 120, 240 mg.kg<sup>-1</sup>) of supplemental iron and 3 levels (0, 1.0, and 5.0 mg.kg<sup>-1</sup>) of supplemental iodine was utilized.</div></div><div><h3>Results</h3><div>Dietary iodine did not affect bird performance. However, egg laying and feed intake decreased, and feed conversion ratio increased in the absence of supplemental iron. Diets without supplemental iron increased abnormal eggs along with decreasing the eggshell quality (weight, thickness, strength, and calcium content) and disturbing the serum and egg yolk lipids. This paralleled the reduction of serum thyroxine and triiodothyronine and disruption of the oxidative status in the serum and eggs. The highest production of abnormal eggs and the lowest eggshell weight and thickness occurred with diet containing no supplemental iron and 0.5 mg.kg<sup>-1</sup> of iodine. The application of iron and iodine resulted in elevated levels of these elements in the eggs, with the increase in iodine content demonstrating a dose-dependent relationship. Moreover, while iron contents were constant between 8 and 16 wk, iodine contents showed increasing trends with 0.5 mg.kg<sup>-1</sup> of iodine.</div></div><div><h3>Conclusion</h3><div>It is advisable to enhance the diet with 120 mg.kg<sup>-1</sup> of organic iron and 1 mg.kg<sup>-1</sup> of organic iodine to ensure optimal performance, egg quality, and overall health in laying hens.</div></div>","PeriodicalId":73997,"journal":{"name":"Journal of trace elements and minerals","volume":"11 ","pages":"Article 100211"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}