Journal of ocular diseases and therapeutics最新文献

{"title":"Effect of Cationic Antimicrobial Protein CAP37 on Cytokine Profile during Corneal Wound Healing.","authors":"Anne Kasus-Jacobi,&nbsp;Gina L Griffith,&nbsp;Megan Lerner,&nbsp;H Anne Pereira","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The cationic antimicrobial protein of 37 kDa (CAP37) mediates proliferation, migration, and adhesion of human corneal epithelial cells and promotes corneal re-epithelialization in mouse. The purpose of this study was to investigate the cytokine profile following abrasion of the corneal epithelium, and to identify the cytokines modulated by topical treatment with CAP37 to determine the mechanism by which CAP37 contributes to the recruitment of inflammatory cells and healing of the cornea. The corneal epithelium in mouse eyes was removed and wounds were treated with a saline vehicle or human recombinant CAP37. Wounds were visualized with fluoresce in staining at 0, 16, 24 and 48 h. Mouse corneas were excised at 0, 6, 16, 24 and 48 h post corneal abrasion. The excised corneas were analyzed by immunohistochemistry for re-epithelialization and infiltration of inflammatory cells while the expression profiles of thirty-two cytokines were investigated by multiplex analysis. Results corroborating previous studies showed accelerated wound closure in corneas treated with CAP37 compared to those treated with the saline vehicle. Immunohistochemistry revealed less neutrophil infiltration in CAP37-treated corneas when compared to controls at 24 h. By 48 h post-wounding, histological analysis revealed more staining for neutrophils than the staining observed in the controls. Modulation of cytokine expression occurred for the majority of the cytokines tested at the time of corneal abrasion, during re-epithelialization, and/or by CAP37 treatment. Cytokines monocyte chemoattractant protein-1 (MCP-1) and regulated on activation, normal T cell expressed and secreted (RANTES) were induced during re-epithelialization, at the early 16 h time point. Interleukin 6 (IL-6), leukemia inhibitory factor (LIF), granulocyte colony-stimulating factor (G-CSF), IL-12p70, macrophage inflammatory protein 1 beta (MIP-1β), and interferon gamma-induced protein 10 (IP-10) were induced at 24 h and unchanged during CAP37 treatment. By contrast, IL-15, monokine induced by gamma interferon (MIG), keratinocyte-derived cytokine (KC), tumor necrosis factor alpha (TNF-α), MIP-1α, IL-1β, and macrophage colony-stimulating factor (M-CSF) were modulated by CAP37 treatment. In general, CAP37 appeared to decrease pro-inflammatory cytokines at 24 h and increase them at 48 h when compared to the control group. These data demonstrate that CAP37 modulates the production of cytokines in the cornea and suggest that limiting the number of neutrophils recruited during the early inflammatory phase may support corneal re-epithelialization.</p>","PeriodicalId":73874,"journal":{"name":"Journal of ocular diseases and therapeutics","volume":"5 ","pages":"19-27"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4f/e0/nihms949667.PMC6287921.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10333744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency of 0.01% Dexamethasone Solution in Comprehensive Therapy of Dry Eye Disease","authors":"Brzheskiy Vladimir, P. Vladimir, Kalinina Irina, Kalinina Natalia","doi":"10.12974/2309-6136.2016.04.01.1","DOIUrl":"https://doi.org/10.12974/2309-6136.2016.04.01.1","url":null,"abstract":"Introduction: At present anti-inflammatory therapy of patients with dry eye disease is based mainly on glucocorticoids' instillations. In spite of the fact that dexamethasone in officinal dosage (0.1%) has a marked local anti-inflammatory effect, its wide use is limited by the presence of a destructive process in the cornea. Taking this into account, the authors developed a drug containing 0.01% dexamethasone phosphate in combinations with 6% polyvinylpyrolidone and 1.5-5.5% dextrose solution.\u0000Objective: To study the impact of the developed medication on the inflammatory process dynamics in the tissues of the eye surface in patients with dry eye disease of various etiology.\u0000Materials and Methods: The material of this study was based on the results of the examination and treatment of 25 volunteers (50 eyes) with corneal-conjunctival xerosis developed on the background of 7 cases of meibomian blepharitis (14 eyes), 8 cases of perimenopause (16 eyes), and 10 cases of Sjögren's syndrome (20 eyes).\u0000All patients used the developed medication in the form of eye drops 3-4 times daily on the background of tear replacement therapy. Prior to the therapy and on day 28 of the study the following parameters were assessed, lower tear meniscus index, precorneal tear film production, stability and osmolarity, staining of eye surface epithelium with sodium fluoresce in solution, as well as bengal rose and lissamine green. The quantity of cytokines was determined in the tear fluid and blood plasma with ELISA method: interleukins-1β, 2, 4, 6, 8, 10, 17A, interleukin 1 receptor antagonist, TNF α, interferons α and γ. Besides, all the subjects were asked to fill in a questionnaire to evaluate subjective signs of the ocular surface epithelium xerosis or Ocular Surface Disease Index (OSDI).\u0000Results: By day 28 of the study statistically valid increase of the tear meniscus index, precorneal tear film stability, main and total tear production and decrease of tear film osmolarity were observed. Besides, the staining degree of the ocular surface epithelium with bengal rose and lissamine green (van Bijsterv?ld scale) and with sodium fluoresce in (Oxford scale) decreased. Also, the positive dynamics of the objective parameters of the ocular surface epithelium is confirmed by the subjects' patients evaluation of their quality of life.\u0000Conclusion: The results of the study performed prove the high clinical efficiency of the developed medication that has a marked local anti-inflammatory effect in the therapy of dry eye disease of various etiology. ","PeriodicalId":73874,"journal":{"name":"Journal of ocular diseases and therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66337178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simfield: A Computer Simulated Visual Field Test to Screen for Glaucoma","authors":"J. Hellman, Marie E. Perrone, Diego Altamirano, L. Farrokh-siar, A. Anchala, S. Ksiazek","doi":"10.12974/2309-6136.2016.04.01.2","DOIUrl":"https://doi.org/10.12974/2309-6136.2016.04.01.2","url":null,"abstract":"Objective: To evaluate the efficacy of a computer simulated visual field test (Simfield) as a screening tool for glaucoma.\u0000Design/Participants/Methods: 36 glaucoma or glaucoma suspect patients (53 eyes) from one ophthalmology clinic between October 2013 and May 2014 used the Simfield program within six months of a reliable performance on a 24-2 SITA standard Humphrey Visual Field Analyzer Exam. The exam measured threshold values at the same 54 points as the 24-2 SITA standard software as well as false positives, false negatives, and fixation losses. Two glaucoma specialists and one general ophthalmologist analyzed the Simfield results in masked fashion and determined whether there was evidence of a glaucomatous defect in any of the 4 quadrants of each field. These results were compared to the corresponding HFA tests to determine sensitivity and specificity.\u0000Results: The sensitivity of Simfield ranged from 51-76% and the specificity was 67-88%. In a sub analysis that eliminated mild defects, defined as defects in a field with mean deviation < 7.0, sensitivity improved to 75-91% and specificity was 69-91%. The average false positive rate was 5.2%, the average false negative rate was 3.7%, and the average fixation loss rate was 27.7%.\u0000Conclusions: Simfield is an effective test for identifying moderate to severe glaucomatous visual field loss and can be accessed from any home computer. While the cost to detect glaucoma in one patient using current screening methods is estimated at $1000, Simfield can be used for free anywhere that a computer is available. ","PeriodicalId":73874,"journal":{"name":"Journal of ocular diseases and therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66337378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Normative Values and Contralateral Comparison of Anterior Chamber Depth Measured by Orbscan II in an Iranian Population ","authors":"Hamid Gharaee, M. Abrishami, M. Shafiee, Samira Hasan Zadeh, Mostafa Abrishami, Pardis Eghbali, Parisa Eghbali","doi":"10.12974/2309-6136.2014.02.01.9","DOIUrl":"https://doi.org/10.12974/2309-6136.2014.02.01.9","url":null,"abstract":"Purpose: The objective of study was (i) to determine the normative values of anterior chamber depth (ACD) with Orbscan II Topography System and (ii) to compare right and left eyes data in the normal young population. Methods: A total of 1001 healthy participants aged 18-45 years participated in this observational cross-sectional study. The study population consisted of 616 female and 385 male subjects. The ACD was measured with the Orbscan II. In our study, ACD was defined as the distance between the corneal epithelium and the anterior lens surface. The differences between genders, between right and left eyes and age-related changes were evaluated. Statistical analyses were performed using student t test. Results: The average ACD in our study population was recorded as 3.653 ± 0.306 mm (median: 3.670 mm, mode: 3.70 mm, minimum: 2.35 mm and maximum: 6.60 mm, 95% Confidence interval: 3.640 to 3.666). The ACD was 3.64±0.30 mm in males and 3.67±0.32 mm in females which was statistically different between genders (P < 0.01). There was a much similarity in ACD values in both eyes. ACD was decreased with age (r= -0.250, p<0.001). Conclusion: Detailed description and analysis of ACD with Orbscan demonstrated that the obtained average value of ACD was higher in female than male and decreased slightly with increasing age. There was no difference between the eyes. ","PeriodicalId":73874,"journal":{"name":"Journal of ocular diseases and therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66337531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}