Journal of neuroparasitology最新文献_第2页

Alterations in the Brain Transcriptome in Plasmodium Berghei ANKA Infected Mice. 伯氏疟原虫ANKA感染小鼠脑转录组的改变

Journal of neuroparasitology Pub Date : 2010-10-01

Mahalia S Desruisseaux, Dumitru A Iacobas, Sanda Iacobas, Shankar Mukherjee, Louis M Weiss, Herbert B Tanowitz, David C Spray

{"title":"Alterations in the Brain Transcriptome in Plasmodium Berghei ANKA Infected Mice.","authors":"Mahalia S Desruisseaux, Dumitru A Iacobas, Sanda Iacobas, Shankar Mukherjee, Louis M Weiss, Herbert B Tanowitz, David C Spray","doi":"","DOIUrl":"","url":null,"abstract":"We have used cDNA microarrays to compare gene expression profiles in brains from normal mice to those infected with the ANKA strain of Plasmodium berghei, a model of cerebral malaria. For each of three brains in each group, we computed ratios of all quantifiable genes with a composite reference sample and then computed ratios of gene expression in infected brains compared to untreated controls. Of the almost 12,000 unigenes adequately quantified in all arrays, approximately 3% were significantly downregulated (P < 0.05, ≥ 50% fold change) and about 7% were upregulated. Upon inspection of the lists of regulated genes, we identified a high number encoding proteins of importance to normal brain function or associated with neuropathology, including genes that encode for synaptic proteins or genes involved in cerebellar function as well as genes important in certain neurological diseases such as Alzheimer's disease or autism. These results emphasize the important impact of malarial infection on gene expression in the brain and provide potential biomarkers that may provide novel therapeutic targets to ameliorate the neurological sequelae of this infection.","PeriodicalId":73863,"journal":{"name":"Journal of neuroparasitology","volume":"1 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587055/pdf/nihms435859.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9675786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alterations in the Brain Transcriptome in Plasmodium Berghei ANKA Infected Mice. 伯氏疟原虫ANKA感染小鼠脑转录组的改变

Journal of neuroparasitology Pub Date : 2010-10-01 DOI: 10.4303/JNP/N100803

M. Desruisseaux, D. Iacobas, S. Iacobaş, Shankar Mukherjee, L. Weiss, H. Tanowitz, D. Spray

{"title":"Alterations in the Brain Transcriptome in Plasmodium Berghei ANKA Infected Mice.","authors":"M. Desruisseaux, D. Iacobas, S. Iacobaş, Shankar Mukherjee, L. Weiss, H. Tanowitz, D. Spray","doi":"10.4303/JNP/N100803","DOIUrl":"https://doi.org/10.4303/JNP/N100803","url":null,"abstract":"We have used cDNA microarrays to compare gene expression profiles in brains from normal mice to those infected with the ANKA strain of Plasmodium berghei, a model of cerebral malaria. For each of three brains in each group, we computed ratios of all quantifiable genes with a composite reference sample and then computed ratios of gene expression in infected brains compared to untreated controls. Of the almost 12,000 unigenes adequately quantified in all arrays, approximately 3% were significantly downregulated (P < 0.05, ≥ 50% fold change) and about 7% were upregulated. Upon inspection of the lists of regulated genes, we identified a high number encoding proteins of importance to normal brain function or associated with neuropathology, including genes that encode for synaptic proteins or genes involved in cerebellar function as well as genes important in certain neurological diseases such as Alzheimer's disease or autism. These results emphasize the important impact of malarial infection on gene expression in the brain and provide potential biomarkers that may provide novel therapeutic targets to ameliorate the neurological sequelae of this infection.","PeriodicalId":73863,"journal":{"name":"Journal of neuroparasitology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71099679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Trypanosoma cruzi-Derived Neurotrophic Factor: Role in Neural Repair and Neuroprotection. 克氏锥虫衍生神经营养因子:在神经修复和神经保护中的作用。

Journal of neuroparasitology Pub Date : 2010-07-26 DOI: 10.4303/jnp/N100507

Marina V Chuenkova, Mercio Pereiraperrin

{"title":"Trypanosoma cruzi-Derived Neurotrophic Factor: Role in Neural Repair and Neuroprotection.","authors":"Marina V Chuenkova, Mercio Pereiraperrin","doi":"10.4303/jnp/N100507","DOIUrl":"https://doi.org/10.4303/jnp/N100507","url":null,"abstract":"Some patients infected with the parasite Try-panosoma cruzi develop chronic Chagas' disease, while others remain asymptomatic for life. Although pathological mechanisms that govern disease progression remain unclear, the balance between degeneration and regeneration in the peripheral nervous system seems to contribute to the different clinical outcomes. This review focuses on certain new aspects of host-parasite interactions related to regeneration in the host nervous system induced by the trans-sialidase of T. cruzi, also known as a parasite-derived neurotrophic factor (PDNF). PDNF plays multiple roles in T. cruzi infection, ranging from immunosuppression to functional mimicry of mammalian neurotrophic factors and inhibition of apoptosis. PDNF affinity to neurotrophin Trk receptors provide sustained activation of cellular survival mechanisms resulting in neuroprotection and neuronal repair, resistance to cytotoxic insults and enhancement of neuritogenesis. Such unique PDNF-elicited regenerative responses likely prolong parasite persistence in infected tissues while reducing neuropathology in Chagas' disease.","PeriodicalId":73863,"journal":{"name":"Journal of neuroparasitology","volume":" ","pages":"55-60"},"PeriodicalIF":0.0,"publicationDate":"2010-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3092389/pdf/nihms254024.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29883726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Baylisascaris Procyonis Neural Larva Migrans in an Infant in New York City. 纽约市一个婴儿的贝氏蛔虫原yonis神经幼虫迁移。

Journal of neuroparasitology Pub Date : 2010-01-01 DOI: 10.4303/jnp/N100502

Jason E Perlman, Kevin R Kazacos, Gavin H Imperato, Rajen U Desai, Susan K Schulman, Jon Edwards, Lucy R Pontrelli, Fabiana S Machado, Herbert B Tanowitz, Norman A Saffra

引用次数: 24

Increasing Intracerebral Infections Caused by Free-Living Amebae in the United States and Worldwide 在美国和世界范围内，由自由生活的阿米巴虫引起的脑内感染日益增加

Journal of neuroparasitology Pub Date : 2010-01-01 DOI: 10.4303/JNP/N100801

J. Diaz

{"title":"Increasing Intracerebral Infections Caused by Free-Living Amebae in the United States and Worldwide","authors":"J. Diaz","doi":"10.4303/JNP/N100801","DOIUrl":"https://doi.org/10.4303/JNP/N100801","url":null,"abstract":"Free-living amebae of the genera Acanthamoeba, Balamuthia, Naegleria, and Sappinia are rare causes of infectious diseases in humans with the exception of Acanthamoeba keratitis (AK) which is reported in millions of soft contact lens wearers worldwide each year. Unlike several Acanthamoeba species, only one species of Naegleria, N. fowleri, is known to infect humans by causing an acute, fulminant, usually lethal, central nervous system (CNS) infection, known as primary amebic meningoencephalitis (PAM). Balamuthia mandrillaris, another opportunistic, free-living ameba, is, like Acanthamoeba spp., capable of causing skin lesions and granulomatous amebic encephalitis (GAE) in individuals with compromised or competent immune systems, who inhale infective cysts or develop indolent, granulomatous skin lesions in soil-contaminated wounds. Lastly, Sappinia pedata, a recently identified free-living ameba that lives in soil and animal and reptile feces, has caused a single case of nongranulomatous amebic encephalitis in an immunocompetent Texas farmer. CNS infections caused by these ubiquitous organisms remain rare, but are, nevertheless, increasing today in the US and worldwide due to a combination of environmental and host susceptibility factors. The purpose of this review will be to describe the current epidemiology, pathophysiology, clinical manifestations, diagnosis, management, and prevention of free-living amebic infections of the CNS.","PeriodicalId":73863,"journal":{"name":"Journal of neuroparasitology","volume":"1 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71098960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Validity of Modelling Cerebral Malaria in Mice: Argument and Counter Argument 小鼠脑疟疾模型的有效性:论证与反论证

Journal of neuroparasitology Pub Date : 2010-01-01 DOI: 10.4303/JNP/N100601

A. Taylor-Robinson

{"title":"Validity of Modelling Cerebral Malaria in Mice: Argument and Counter Argument","authors":"A. Taylor-Robinson","doi":"10.4303/JNP/N100601","DOIUrl":"https://doi.org/10.4303/JNP/N100601","url":null,"abstract":"The clinical manifestations of Plasmodium falciparum infection of humans that are collectively recognised as cerebral malaria produce profound changes in mental status and induce coma. The histopathological hallmark of this encephalopathy is the sequestration of cerebral capillaries and venules with both infected and uninfected erythrocytes. The underlying cause of cerebral malaria is the subject of vigorous debate. A major reason for this is that human brain tissue is only available post mortem. In order to dissect the pathology of this acute disease, therefore, a number of models using murine malarias have been developed. While these have undoubtedly proved useful in helping to identify immunological mechanisms involved, recognition of the differences between the pathological processes during cerebral malaria in mice and man has led to some researchers questioning the validity of extrapolating findings from mouse models to the human condition with a view to informing therapeutic interventions. In turn, this has provoked lively debate within the malaria research community. This commentary sets out our current understanding of cerebral disease in humans and evaluates what meaningful contribution the study of mouse models has made to this knowledge.","PeriodicalId":73863,"journal":{"name":"Journal of neuroparasitology","volume":"1 1","pages":"45-49"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71098899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Trypanosoma cruzi-Induced Central Nervous System Alterations: From the Entry of Inflammatory Cells to Potential Cognitive and Psychiatric Abnormalities 克氏锥虫诱导的中枢神经系统改变:从炎症细胞的进入到潜在的认知和精神异常

Journal of neuroparasitology Pub Date : 2010-01-01 DOI: 10.4303/JNP/N100901

A. A. Silva, G. Pereira, A. S. Souza, Rafael Rodrigues Silva, M. Rocha, J. Lannes-Vieira

{"title":"Trypanosoma cruzi-Induced Central Nervous System Alterations: From the Entry of Inflammatory Cells to Potential Cognitive and Psychiatric Abnormalities","authors":"A. A. Silva, G. Pereira, A. S. Souza, Rafael Rodrigues Silva, M. Rocha, J. Lannes-Vieira","doi":"10.4303/JNP/N100901","DOIUrl":"https://doi.org/10.4303/JNP/N100901","url":null,"abstract":"Trypanosoma cruzi, a protozoan parasite and the causative agent of Chagas disease, is capable of inducing meningoencephalitis. Independent of the progression from acute to chronic myocarditis observed in immunocompetent T. cruzi-infected patients, inflammation of the central ner- vous system (CNS) self-resolves during acute infection. In contrast, in chronically infected immunocompromised Cha- gas disease patients, the CNS is a major site of reactivation, which can lead to severe and frequently fatal meningoen- cephalitis. More than one hundred years after the discovery of Chagas disease, many questions concerning the molecu- lar mechanisms involved in the induction and resolution of T. cruzi-provoked meningoencephalitis remain unanswered. The study of murine models that reproduce crucial aspects of T. cruzi-elicited CNS inflammation has not only shed light on some of these questions, but it has also raised additional ones. Here, we discuss our results in the context of the cur- rent literature, questioning the involvement of CNS alter- ations caused by the inflammation and parasite in the behav- ioral abnormalities observed during T. cruzi infection.","PeriodicalId":73863,"journal":{"name":"Journal of neuroparasitology","volume":"1 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71099362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Circulating Antigen Levels Follow Post-Treatment Evolution of Subarachnoid Neurocysticercosis 循环抗原水平跟踪蛛网膜下腔神经囊虫病治疗后的演变

Journal of neuroparasitology Pub Date : 2010-01-01 DOI: 10.4303/JNP/N100804

H. García, P. Dorny, Yesenia Castillo, E. Pretell, Silvia Rodriguez, L. Mija, Armando E. Gonzalez, R. Gilman, V. Tsang, J. Brandt

引用次数: 12

Host Cell Preference of Toxoplasma gondii Cysts in Murine Brain: A Confocal Study. 小鼠脑内刚地弓形虫囊的宿主细胞偏好:共聚焦研究。

Journal of neuroparasitology Pub Date : 2010-01-01 DOI: 10.4303/jnp/N100505

T C Melzer, H J Cranston, L M Weiss, S K Halonen

{"title":"Host Cell Preference of Toxoplasma gondii Cysts in Murine Brain: A Confocal Study.","authors":"T C Melzer, H J Cranston, L M Weiss, S K Halonen","doi":"10.4303/jnp/N100505","DOIUrl":"https://doi.org/10.4303/jnp/N100505","url":null,"abstract":"Toxoplasma gondii is a protozoan parasite that is widely prevalent in humans and typically results in a chronic infection characterized by cysts located predominantly in the central nervous system. In immunosuppressed hosts, such as patients with HIV infection, the infection can be reactivated from the cysts in the brain resulting in a severe and potentially fatal encephalitis. Studies suggest that the chronic infection may also have neuropathological and behavioral effects in immune competent hosts. An improved understanding of tissue cyst behavior is of importance for understanding both the reactivation as well as the neurophysiological consequences of chronic infection. In vivo studies have identified neurons as host cells for cysts but in vitro studies have found that astrocytes can also foster development of the cysts. In this study we have addressed the question of which neural cell tissue cysts of T. gondii reside during chronic infection using a mouse model. Mice were infected with Me49 Strain T. gondii and the intracellular localization of the cysts analyzed during the development and establishment of a chronic infection at 1, 2, and 6 months post infection. Brains were fixed, cryosectioned, and stained with FITC-Dolichos biflorans to identify the Toxoplasma cysts and they were labeled with cell specific antibodies to neurons or astrocytes and then analyzed using confocal fluorescence microscopy. Cysts were found to occur almost exclusively in neurons throughout chronic infection. No cysts were identified in astrocytes, using the astrocyte marker, GFAP. Astrocyte interactions with neuronal-cysts, however, were frequently observed.","PeriodicalId":73863,"journal":{"name":"Journal of neuroparasitology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4303/jnp/N100505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30204420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 99

Baylisascaris Procyonis Induced Diffuse Unilateral Subacute Neuroretinitis in New York City. 纽约贝氏蛔虫引起的弥漫性单侧亚急性神经视网膜炎。

Journal of neuroparasitology Pub Date : 2010-01-01 DOI: 10.4303/jnp/N100401

Norman A Saffra, Jason E Perlman, Rajen U Desai, Kevin R Kazacos, Christina M Coyle, Fabiana S Machado, Sanjay R Kedhar, Michael Engelbert, Herbert B Tanowitz

引用次数: 12