Journal of extracellular biology最新文献

Correction to “Exploring the Potential of in Vitro Extracellular Vesicle Generation in Reproductive Biology” 对“探索体外细胞外囊泡生成在生殖生物学中的潜力”的更正

Journal of extracellular biology Pub Date : 2025-05-29 DOI: 10.1002/jex2.70061

引用次数: 0

Correction to “Prostate-Derived Circulating microRNAs Add Prognostic Value to Prostate Cancer Risk Calculators” 对“前列腺衍生循环microrna增加前列腺癌风险计算器的预后价值”的更正

Journal of extracellular biology Pub Date : 2025-05-29 DOI: 10.1002/jex2.70060

引用次数: 0

Scalable, High-Throughput Isolation of Extracellular Vesicles Using Electrokinetic-Assisted Mesh Filtration: ExoFilter 可扩展的，高通量分离细胞外囊泡使用电动辅助网状过滤：exfilter

Journal of extracellular biology Pub Date : 2025-05-28 DOI: 10.1002/jex2.70054

KangMin Lee, Minju Bae, YongWoo Kim, SoYoung Jeon, Sujin Kang, Wonjong Rhee, Sehyun Shin

{"title":"Scalable, High-Throughput Isolation of Extracellular Vesicles Using Electrokinetic-Assisted Mesh Filtration: ExoFilter","authors":"KangMin Lee, Minju Bae, YongWoo Kim, SoYoung Jeon, Sujin Kang, Wonjong Rhee, Sehyun Shin","doi":"10.1002/jex2.70054","DOIUrl":"https://doi.org/10.1002/jex2.70054","url":null,"abstract":"As extracellular vesicles (EVs) are increasingly recognized for their superior functions for therapeutics, the need for large-scale EV isolation technology is becoming more critical for clinical and industrial applications. Most existing EV isolation methods are optimized for small-scale laboratory samples, limiting their efficiency and scalability for large-scale production. Here, an electrokinetic-assisted filtration system (ExoFilter), which introduces charge interaction into physical mesh flow filtration, is proposed as a new candidate to address the challenges of scalable EV isolation. The hybrid filtration system demonstrates outstanding high-throughput EV isolation performance (a flux of ∼750 mL/min) using only a coarse physical filter by electrokinetically arresting EVs flowing through the filter lattice. Furthermore, the recovery efficiency of ExoFilter, analysed based on the ELISA results, was found to be approximately 98%, demonstrating the filter's exceptional efficiency in EV isolation. Additionally, ExoFilter enables the rapid isolation of EVs from small samples as little as 200 µL, facilitating quick and easy blood-based EV research. Furthermore, low-molecular-weight albumin from plasma samples was effectively removed. The high-throughput and high-efficiency characteristics of ExoFilter make it well-suited for scalable EV production, offering greater convenience for various clinical applications.","PeriodicalId":73747,"journal":{"name":"Journal of extracellular biology","volume":"4 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jex2.70054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conference & School on Extracellular vesicles and Nanoparticles 2024 (CSEVP-2024) 2024年细胞外囊泡和纳米颗粒会议与学校（CSEVP-2024）

Journal of extracellular biology Pub Date : 2025-05-22 DOI: 10.1002/jex2.70046

引用次数: 0

Control of Physical and Biochemical Parameters Influencing Exogeneous Cargo Protein Association to Extracellular Vesicles Using Lipid Anchors Enables High Loading and Effective Intracellular Delivery 利用脂质锚定控制影响外源货物蛋白与细胞外囊泡关联的物理和生化参数，实现高负载和有效的细胞内递送

Journal of extracellular biology Pub Date : 2025-05-14 DOI: 10.1002/jex2.70048

Antonin Marquant, Jade Berthelot, Claudia Bich, Zeineb Ibn Elfekih, Laurianne Simon, Baptiste Robin, Joël Chopineau, David Tianpei Wang, Samuel Jay Emerson, Aijun Wang, Clément Benedetti, Simon Langlois, Laurence Guglielmi, Pierre Martineau, Anne Aubert-Pouëssel, Marie Morille

{"title":"Control of Physical and Biochemical Parameters Influencing Exogeneous Cargo Protein Association to Extracellular Vesicles Using Lipid Anchors Enables High Loading and Effective Intracellular Delivery","authors":"Antonin Marquant, Jade Berthelot, Claudia Bich, Zeineb Ibn Elfekih, Laurianne Simon, Baptiste Robin, Joël Chopineau, David Tianpei Wang, Samuel Jay Emerson, Aijun Wang, Clément Benedetti, Simon Langlois, Laurence Guglielmi, Pierre Martineau, Anne Aubert-Pouëssel, Marie Morille","doi":"10.1002/jex2.70048","DOIUrl":"https://doi.org/10.1002/jex2.70048","url":null,"abstract":"Despite biomolecule delivery is a natural function of extracellular vesicles (EVs), low loading of exogenous macromolecules such as proteins into EVs limits their interest as convincing protein delivery systems for health applications. In this context, lipid-anchorage of exogenous cargo into EV membrane recently emerged as a promising option to enable their vectorisation into cells. Nevertheless, this option was not explored for protein intracellular delivery, and further characterisation of critical parameters governing the association of a lipid-anchored cargo protein to EVs is still needed to confirm the relevance of this anchorage strategy. Therefore, we sought to identify these parameters in a precise and quantitative manner, using bulk and single nanoparticle analysis methods to identify protein loading capacity and subsequent intracellular delivery. We identified incubation temperature, cargo concentration, lipid anchor (LA) structure (lipid nature, linker) and EV origin as critical factors influencing maximal EV loading capacity. Precise control of these parameters enabled to load cargo protein close to EV saturation without hindering cellular delivery. The structural properties of LA influenced not only cargo protein/EV association but also intracellular delivery into different carcinoma cell lines. By thoroughly characterising Lipid-PEG-protein anchorage, this study evidences the interest of this tunable and controllable approach for efficient EV protein delivery.","PeriodicalId":73747,"journal":{"name":"Journal of extracellular biology","volume":"4 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jex2.70048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting the Nanoflow Cytometric Quantification of Extracellular Vesicles Under the Framework of ICH Q14 Guidelines 在ICH Q14指南框架下重新审视细胞外囊泡的纳米流式细胞术定量

Journal of extracellular biology Pub Date : 2025-05-08 DOI: 10.1002/jex2.70050

Ganghui Li, Qizhe Cai, Yanan Dong, Xiang Li, Xi Qin, Miaomiao Xue, Haifeng Song, Yi Wang

{"title":"Revisiting the Nanoflow Cytometric Quantification of Extracellular Vesicles Under the Framework of ICH Q14 Guidelines","authors":"Ganghui Li, Qizhe Cai, Yanan Dong, Xiang Li, Xi Qin, Miaomiao Xue, Haifeng Song, Yi Wang","doi":"10.1002/jex2.70050","DOIUrl":"https://doi.org/10.1002/jex2.70050","url":null,"abstract":"Nanoflow cytometry (nanoFCM) is an increasingly important analytical procedure in every aspect of extracellular vesicle (EV) research, particularly in the development of EV-based therapeutics. The main objective of this study was to evaluate and optimise the key determinant factors of nanoFCM in the quantification analysis of EVs to ensure its consistency and reliability in the development of EV therapeutic drugs, thereby serving as a potential quality control measure. Our investigation followed the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) Q14 guideline. We revisited the day-to-day practice of nanoFCM measurement for HEK293 cell-derived and milk-derived EVs (mEVs), focusing on optimising particle quantification and identifying risk factors. Initial evaluation of the procedure revealed a considerable lack of consistency and reliability, which was then subjected to extensive optimisation. The key outcomes of this study include: (1) an optimised analytic procedure incorporating Tween-20, which significantly enhanced the precision and accuracy of the nanoFCM measurement and expanded the reportable range; (2) an analytical target profile (ATP) which provides a preliminary standard for future validation of nanoFCM procedures. Overall, this study serves as a foundation for future efforts towards the standardisation of analytical procedures for EV therapeutics.","PeriodicalId":73747,"journal":{"name":"Journal of extracellular biology","volume":"4 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jex2.70050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Extracellular Vesicles in the Pathogenesis of Brain Metastasis 细胞外囊泡在脑转移发病机制中的作用

Journal of extracellular biology Pub Date : 2025-05-06 DOI: 10.1002/jex2.70051

Muhammad Izhar, Maciej S. Lesniak

{"title":"Role of Extracellular Vesicles in the Pathogenesis of Brain Metastasis","authors":"Muhammad Izhar, Maciej S. Lesniak","doi":"10.1002/jex2.70051","DOIUrl":"https://doi.org/10.1002/jex2.70051","url":null,"abstract":"Extracellular vesicles (EVs) are small particles released by various cells, including cancer cells. They play a significant role in the development of different cancers, including brain metastasis. These EVs transport biomolecular materials such as RNA, DNA, and proteins from tumour cells to other cells, facilitating the spread of primary tumours to the brain tissue. EVs interact with the endothelial cells of the blood–brain barrier (BBB), compromising its integrity and allowing metastatic cells to pass through easily. Additionally, EVs interact with various cells in the brain's microenvironment, creating a conducive environment for incoming metastatic cells. They also influence the immune system within this premetastatic environment, promoting the growth of metastatic cells. This review paper focuses on the research regarding the role of EVs in the development of brain metastasis, including their impact on disrupting the BBB, preparing the premetastatic environment, and modulating the immune system. Furthermore, the paper discusses the potential of EVs as diagnostic and prognostic biomarkers for brain metastasis.","PeriodicalId":73747,"journal":{"name":"Journal of extracellular biology","volume":"4 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jex2.70051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143908973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Industrial Scale Production and Characterization of a Whey Fraction Enriched in Extracellular Vesicle Material 富含细胞外囊泡物质的乳清馏分的工业规模生产和表征

Journal of extracellular biology Pub Date : 2025-05-02 DOI: 10.1002/jex2.70044

Søren Roi Midtgaard, Maria Stenum Hansen, Nikolaj Drachmann, Xiaolu Geng, Kristine Ingrid Marie Blans, Manja Mahmens Fabricius Møbjerg, Anny F. Frølund, Jan Trige Rasmussen, Marie Stampe Ostenfeld

{"title":"Industrial Scale Production and Characterization of a Whey Fraction Enriched in Extracellular Vesicle Material","authors":"Søren Roi Midtgaard, Maria Stenum Hansen, Nikolaj Drachmann, Xiaolu Geng, Kristine Ingrid Marie Blans, Manja Mahmens Fabricius Møbjerg, Anny F. Frølund, Jan Trige Rasmussen, Marie Stampe Ostenfeld","doi":"10.1002/jex2.70044","DOIUrl":"https://doi.org/10.1002/jex2.70044","url":null,"abstract":"Human milk serves the sole nutritional role for the developing infant. During lactation, nano-sized extracellular vesicles (EVs) in milk containing a multitude of biologically active components are transferred from mother to offspring. Infant formula (IF) based on cow milk-derived ingredients has been reported to contain reduced levels of EVs as compared to human milk. There is therefore an unmet need to produce large-scale volumes of milk EVs to improve IF composition.Here, we report a scalable industrial production protocol for a bovine whey-derived ingredient that is highly enriched in EV material using a large-scale sequential ceramic membrane filtration setup. Furthermore, we demonstrate a robust and generally applicable analytical approach to determine the relative contributions of EVs and milk fat globule membrane (MFGM) using molar ratios of the membrane-bound proteins butyrophilin (BTN) and CD9 as surrogate markers for MFGM and EVs, respectively. Taken together, our findings provide a basis for comparing bovine milk-containing foods and aid in developing specialized ingredients that can minimize the compositional difference between infant formula and human milk.","PeriodicalId":73747,"journal":{"name":"Journal of extracellular biology","volume":"4 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jex2.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143897166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Extracellular Vesicles Derived From Human Precision-Cut Liver Slices in Metabolic Dysfunction-Associated Steatotic Liver Disease 代谢功能障碍相关脂肪变性肝病中人类精确肝切片细胞外囊泡的表征

Journal of extracellular biology Pub Date : 2025-04-30 DOI: 10.1002/jex2.70043

Yana Geng, Ke Luo, Janine Stam, Dorenda Oosterhuis, Alan R. Gorter, Marius van den Heuvel, Rossella Crescitelli, Vincent E. de Meijer, Justina C. Wolters, Peter Olinga

{"title":"Characterization of Extracellular Vesicles Derived From Human Precision-Cut Liver Slices in Metabolic Dysfunction-Associated Steatotic Liver Disease","authors":"Yana Geng, Ke Luo, Janine Stam, Dorenda Oosterhuis, Alan R. Gorter, Marius van den Heuvel, Rossella Crescitelli, Vincent E. de Meijer, Justina C. Wolters, Peter Olinga","doi":"10.1002/jex2.70043","DOIUrl":"https://doi.org/10.1002/jex2.70043","url":null,"abstract":"Extracellular vesicles (EVs) are cell-produced, membrane-surrounded vesicles that harbour the biological features of donor cells. In the current study, we are the first to isolate and characterize EVs isolated from human precision-cut liver slices (PCLS), obtained from both healthy and metabolic dysfunction-associated steatohepatitis (MASH) cirrhotic livers. PCLS derived from patients can faithfully represent disease conditions in humans. EVs were isolated from human PCLS after incubating in normal medium or modified medium that mimics the pathophysiological environment of metabolic dysfunction associated liver disease (MASLD). MASH PCLS produced higher amounts of EVs compared to healthy PCLS (p < 0.001). Mass spectrometry revealed that around 300 proteins were significantly different in EVs derived from MASH PCLS versus healthy PCLS (FDR < 0.05), irrespective of the type of medium. Significantly changed EV proteins were largely involved in signalling receptor binding function and showed potential in promoting fibrosis. In the liver, these ligand-associated receptors are highly expressed in hepatic stellate cells, and the MASH EVs functionally promoted the activation of hepatic stellate cells. Furthermore, the amounts of EpCAM and ITGA3 in EVs were positively associated with the progression of MASLD, which suggests the use of liver-derived EVs as potential biomarkers for MASLD. Characterization of EVs derived from human PCLS may assist future studies in investigating the pathogenesis and identifying liver-specific EVs as biomarkers of MASLD.","PeriodicalId":73747,"journal":{"name":"Journal of extracellular biology","volume":"4 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jex2.70043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the Influence of Different Serum-Free Culture Conditions on the Production and Function of Natural Killer Cell-Derived Extracellular Vesicles 评估不同无血清培养条件对自然杀伤细胞衍生的细胞外囊泡产生和功能的影响

Journal of extracellular biology Pub Date : 2025-04-25 DOI: 10.1002/jex2.70049

Yunjie Wu, Héloïse Chollet, Amanda Sudworth, Marit Inngjerdingen

{"title":"Evaluating the Influence of Different Serum-Free Culture Conditions on the Production and Function of Natural Killer Cell-Derived Extracellular Vesicles","authors":"Yunjie Wu, Héloïse Chollet, Amanda Sudworth, Marit Inngjerdingen","doi":"10.1002/jex2.70049","DOIUrl":"https://doi.org/10.1002/jex2.70049","url":null,"abstract":"Natural killer (NK) cells are exploited in cellular therapies for cancer. While NK cell therapies are efficient against haematological cancers, it has been difficult to target solid tumours due to low tumour infiltration and a hostile tumour microenvironment. NK-cell derived extracellular vesicles (NK-EVs) target and kill cancer cells in vitro and represent an alternative treatment strategy for solid tumours. To exploit their potential, it is necessary to standardize NK-EV production protocols. Here, we have performed a comparative analysis of EVs from the human NK-92 cell line cultured in five serum-free commercial media optimized for growth of human NK cells and one serum-free medium for growth of lymphocytes. The effect of growing the NK-92 cells in static cell cultures versus shaking flasks was compared. EVs were purified via ultracentrifugation followed by size-exclusion chromatography. We found that there were no significant differences in EV yield from NK-92 cells grown under static or dynamic conditions. However, we found clear differences between the different culture media in terms of EV purity as assessed by the enrichment of the CD63 and CD81 markers in the isolates that translated into their capacity to induce apoptosis of the colon cancer cell line HCT 116. These findings will be instructive for the design of future production protocols for therapeutic NK-cell derived EVs.","PeriodicalId":73747,"journal":{"name":"Journal of extracellular biology","volume":"4 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jex2.70049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0