Advances in Pharmacological and Pharmaceutical Sciences最新文献

{"title":"Drug Utilization Evaluation of Erythropoietin at a Referral Teaching Hospital in Iran","authors":"Iman Karimzadeh, Hanieh Rasekh, Ava Karimian, Mojtaba Shabani-Borujeni, Afsaneh Vazin","doi":"10.1155/2023/6685602","DOIUrl":"https://doi.org/10.1155/2023/6685602","url":null,"abstract":"Objectives. Drug utilization evaluation (DUE) studies aim to survey the appropriateness of drug use. DUE is an executive approach used to improve the use of medications as well as reduce the cost of treatment, ensure drug adequacy, and improve patient safety. The aim of this study was to evaluate the pattern of erythropoietin use, according to standard guidelines, in patients admitted to Namazi Hospital in Shiraz, Iran. Methods. In this descriptive, retrospective study, 230 patients were assessed. All patients who were hospitalized in different wards of Namazi Hospital, affiliated to Shiraz University of Medical Sciences, and received at least three doses of erythropoietin from September 2019 to March 2020 participated in this study. The following standard indicators of erythropoietin use were evaluated through reviewing medical charts of the cohort: drug dose, dosing intervals, route of administration, indication, monitoring of laboratory parameters, drug dose adjustment based on the response rate as well as target hemoglobin ≥12 g/dl, attention to major drug interactions, and administration of injectable or oral iron supplementation during treatment. Results. Most (65.2%) of the participants were male. The mean ± SD age of the patients was 47.55 ± 22.71 years. More than half (51.3%) of the included subjects were hospitalized in the nephrology ward. PDpoetin® and Cinnapoietin® were given to 52.6% and 47.4% of the study participants, respectively. Treatment of anemia due to chronic kidney disease was the most frequent indication of erythropoietin. The time interval of erythropoietin administration was three times a week for 68.3% of the patients. The most frequently administered weekly dose of erythropoietin was 12,000 units. The weekly dose, dose interval, and route of administration of erythropoietin were appropriate in 52.6%, 77.4%, and 100% of the patients, respectively. Dose adjustment based on the response rate, attention to major drug interactions as well as absolute-relative contraindications, and attention to the target hemoglobin ≥12 g/dl to decide whether or not to continue treatment were based on standard guideline in 98.1%, 98.7%, and 93% of the patients, respectively. The sum indexes of erythropoietin use were in line with standard guidelines in 75.84% of the cases. Conclusion. According to our results, in the setting of erythropoietin use in hospitals, physicians need more attention and education in areas such as selecting the proper dose of medication, correct indication of the drug, temporal arrangement of monitoring laboratory items, and the patient’s need for iron supplements.","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"12 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135539646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacovigilance and Adverse Drug Reactions Reporting: Healthcare Providers' Experiences from Southern Highland Tanzania.","authors":"Dorkasi L Mwakawanga,&nbsp;Manase Kilonzi,&nbsp;Erick G Philipo,&nbsp;Aron Martine,&nbsp;Tusaligwe Mbilinyi,&nbsp;Nancy F Kileo,&nbsp;Bryceson Mkinga,&nbsp;Cleopatra Justine Shonyella,&nbsp;Juma A Mohamedi,&nbsp;Aurelia Clement,&nbsp;Davance Mwasomola,&nbsp;Stella E Mushy,&nbsp;Nathanael Sirili","doi":"10.1155/2023/5537592","DOIUrl":"10.1155/2023/5537592","url":null,"abstract":"<p><strong>Purpose: </strong>This exploratory qualitative study aimed to analyze the experiences of healthcare providers (HCPs) in pharmacovigilance (PV) and ADR reporting in the southern highland zone of Tanzania.</p><p><strong>Methods: </strong>In 2022, an exploratory qualitative case study using in-depth interviews (IDIs) was conducted to explore the experiences of PV and ADR reporting among HCPs (doctors, nurses, and pharmacists). The study was carried out in a zonal referral hospital and a regional referral hospital of the Tanzanian southern highlands zone. Inductive-deductive thematic analysis was adopted for data analysis.</p><p><strong>Results: </strong>Participants demonstrated adequate knowledge of PV and its related activities including ADR reporting. Knowing the interactions and wrong medication dosage as sources of ADR, signs, and symptoms, stopping the drug, and treating the symptoms following ADR emerged as subthemes linked with adequate knowledge in identifying and managing ADR. Participants perceived reporting ADR as laborious, posing a subjective burden and that not all ADRs needed to be reported. The latter contributed to limited participation in ADR reporting despite that participants were conversant with both physical and online ADR reporting platforms.</p><p><strong>Conclusion: </strong>Although HCPs are well informed about PV and ADR reporting including the benefits to public health, their involvement in ADR reporting is low. In addition to the ongoing on-the-job training and regular supportive supervision for HCPs to improve the ADR practice, there is still a need to explore other strategies to be used as motives for HCPs to report ADR regularly.</p>","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"2023 ","pages":"5537592"},"PeriodicalIF":2.8,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10593552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50156288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brazilin from <i>Caesalpinia sappan</i> L. as a Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Inhibitor: Pharmacophore-Based Virtual Screening, <i>In Silico</i> Molecular Docking, and <i>In Vitro</i> Studies.","authors":"Muhammad Iqbal,&nbsp;Nur Hasanah,&nbsp;Aimee Detria Arianto,&nbsp;Widya Dwi Aryati,&nbsp;Meidi Utami Puteri,&nbsp;Fadlina Chany Saputri","doi":"10.1155/2023/5932315","DOIUrl":"https://doi.org/10.1155/2023/5932315","url":null,"abstract":"<p><strong>Background: </strong>Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial regulator of low-density lipoprotein cholesterol (LDL-c) levels, as it binds to and degrades the LDL receptor (LDLR) in the lysosome of hepatocytes. Elevated levels of PCSK9 have been linked to an increased LDL-c plasma levels, thereby increasing the risk of cardiovascular disease (CVD), making it an attractive target for therapeutic interventions. As a way to inhibit PCSK9 action, we searched for naturally derived small molecules which can block the binding of PCSK9 to the LDLR.</p><p><strong>Methods: </strong>In this study, we carried out <i>in silico</i> studies which consist of virtual screening using an optimized pharmacophore model and molecular docking studies using Pyrx 0.98. Effects of the candidate compounds were evaluated using <i>in vitro</i> PCSK9-LDLR binding assays kit.</p><p><strong>Results: </strong>Eleven natural compounds that bind to PCSK9 were virtually screened form HerbalDB database, including brazilin. Next, molecular docking studies using Pyrx 0.98 showed that brazilin had the highest binding affinity with PCSK9 at -9.0 (Kcal/mol), which was higher than that of the other ten compounds. Subsequent <i>in vitro</i> PCSK9-LDLR binding assays established that brazilin decreased the binding of PCSK9 to the EGF-A fragment of the LDLR in a dose-dependent manner, with an IC₅₀ value of 2.19 <i>μ</i>M.</p><p><strong>Conclusion: </strong>We have identified brazilin, which is derived from the <i>Caesalpinia sappan</i> herb, which can act as a small molecule inhibitor of PCSK9. Our findings suggest that screening for small molecules that can block the interaction between PCSK9 and the LDLR <i>in silico</i> and <i>in vitro</i> may be a promising approach for developing novel lipid-lowering therapy.</p>","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"2023 ","pages":"5932315"},"PeriodicalIF":2.8,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Direct Compression Property of a Novel Pregelatinized Starch in Paracetamol Tablets.","authors":"Tamrat Balcha Balla,&nbsp;Nisha Mary Joseph,&nbsp;Anteneh Belete","doi":"10.1155/2023/5573176","DOIUrl":"10.1155/2023/5573176","url":null,"abstract":"<p><strong>Background: </strong>Among all the pharmaceutical dosage forms, tablets are still the most preferred and the most commonly used option because of their advantages. The direct compression method of tablet preparation exempts several steps needed in the granulation method. Therefore, the pursuit of better direct compression tablet excipients is evident in contemporary research endeavors. Pregelatinized Taro Boloso-I starch has comparable flow properties and higher compressibility and compactibility than Starch 1500®. However, there is no evidence in the literature regarding the lubricant sensitivity and dilution potential of pregelatinized Taro Boloso-I starch. This study was aimed at performing the <i>in vitro</i> evaluation of paracetamol tablets prepared using pregelatinized Taro Boloso-I starch as a direct compression excipient using paracetamol as a model drug.</p><p><strong>Methods: </strong>Taro Boloso-I starch was pregelatinized, and its properties including amylose to amylopectin ratio, densities, flow properties, swelling power, water solubility index, particle morphology, moisture content, and moisture sorption profile were evaluated. Furthermore, the lubricant sensitivity test, dilution potential study, and compatibility test with the paracetamol drug using ATR spectroscopy were performed. The properties of the directly compressed tablets prepared accordingly were evaluated. The majority of evaluations were performed in comparison with Starch 1500®. <i>Results and Discussion</i>. PGTBIS had a significantly lower amount of amylose than Starch 1500®. In the ATR-IR spectra of the mixture of the paracetamol and pregelatinized PGTBIS, all the major absorbance peaks of the drug were maintained indicating the absence of chemical modifications. PGTBIS showed better flow properties than Starch 1500®. The modified starch was shown to withstand magnesium stearate up to 0.5% concentration.</p><p><strong>Conclusion: </strong>PGTBIS could accommodate higher drug cargo than Starch 1500® with acceptable tablet properties. Accordingly, PGTBIS starch could be taken as a potential direct compression excipient.</p>","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"2023 ","pages":"5573176"},"PeriodicalIF":2.8,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10567489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41188025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of <i>G. applanatum</i> Crude Polysaccharide Extract on Proinflammatory Cytokines and Proapoptotic Caspases in HeLa Cell Line: An <i>In Vitro</i> Study.","authors":"Qurrotu A'yun,&nbsp;Raden Joko Kuncoroningrat Susilo,&nbsp;Suhailah Hayaza,&nbsp;Nur'aini Fikriyah,&nbsp;Fina Syifa'una Musthoza,&nbsp;Ufairanisa Islamatasya,&nbsp;Aulia Umi Rohmatika,&nbsp;Dwi Winarni,&nbsp;Sri Puji Astuti Wahyuningsih,&nbsp;Ruey-An Doong,&nbsp;Deya Karsari,&nbsp;Aristika Dinar Yanti,&nbsp;Mochammad Zakki Fahmi,&nbsp;Win Darmanto","doi":"10.1155/2023/3593295","DOIUrl":"https://doi.org/10.1155/2023/3593295","url":null,"abstract":"<p><p>Polysaccharide extracts exhibit promise as potential anticancer agents. Among the fungi rich in polysaccharide content, <i>G. applanatum</i> stands out; however, its anticancer activity necessitates further investigation. This study aims to explore the impact of <i>G. applanatum</i> crude polysaccharide (GACP) extract by assessing its effects on cell viability, levels of proinflammatory cytokines such as TNF-<i>α</i>, IFN-<i>γ</i>, IL-2, and IL-12, and levels of proapoptotic markers including caspase-3 and caspase-9, as well as the percentages of necrosis and apoptosis in the HeLa cell line. Employing the HeLa cell line as a research model, four groups were studied: KN (media and DMSO), <i>K</i>+ (doxorubicin 10 <i>μ</i>g/mL), P1 (<i>G. applanatum</i> extract 200 <i>μ</i>g/mL), and P2 (<i>G. applanatum</i> extract 400 <i>μ</i>g/mL). The <i>G. applanatum</i> extract was obtained via boiling distilled water. Anticancer activity was evaluated through the MTT test (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) conducted over three treatment durations (24, 48, and 72 hours). Cytokine levels and caspase-3 and caspase-9 levels were assessed using the ELISA test. Cell apoptosis was determined using the Annexin V-PI biomarker and analyzed through flow cytometry. The MTT test exhibited optimal results at the 48-hour treatment mark. Cytokine level analysis revealed significant reductions in TNF-<i>α</i>, IFN-<i>γ</i>, IL-2, and IL-12 levels (<i>p</i> < 0.005). Concurrently, caspase-3 and caspase-9 levels exhibited substantial increases (<i>p</i> < 0.005). Flow cytometry highlighted the highest percentage of apoptosis in HeLa cells. In conclusion, <i>G. applanatum</i>'s polysaccharide extract demonstrates potential as an anticancer and therapeutic agent for cancer treatment.</p>","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"2023 ","pages":"3593295"},"PeriodicalIF":2.8,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41107097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Analysis, Antimalarial Properties, and Acute Toxicity of Aqueous Extracts of Trisamo and Jatu-Phala-Tiga Recipes.","authors":"Arisara Phuwajaroanpong,&nbsp;Prapaporn Chaniad,&nbsp;Walaiporn Plirat,&nbsp;Atthaphon Konyanee,&nbsp;Abdi Wira Septama,&nbsp;Chuchard Punsawad","doi":"10.1155/2023/6624040","DOIUrl":"https://doi.org/10.1155/2023/6624040","url":null,"abstract":"<p><p>Drug resistance remains a significant problem that threatens antimalarial drug treatment. Hence, the challenge is to find new effective antimalarial drugs. Based on our previous study, aqueous extracts of trisamo (TSM) and jatu-phala-tiga (JPT) had good <i>in vitro</i> antimalarial activities, and these recipes contain multiple beneficial pharmacological effects that could be useful for malaria therapy. Therefore, this study aimed to investigate the antimalarial activity and toxicity of the aqueous extracts of TSM and JPT in mouse models. The aqueous extractions were carried out using the decoction method. Compound identification was conducted using LC-QTOF-MS analysis. The antimalarial activities of TSM and JPT at doses 200, 400, and 600 mg/kg were evaluated against <i>Plasmodium berghei</i> ANKA infection using a four-day suppressive test. The toxic effects of oral administration of the extracts at 2 g/kg dose were determined using an acute toxicity test. The chemical constituents of TSM contained 83 compounds, whereas JPT contained 84 compounds. All doses of the extracts exhibited a significant suppression (<i>p</i> < 0.05) of the parasite compared to the negative control in a four-day test. The maximum activities were observed at 600 mg/kg dose with 67.02% suppression for TSM and 79.34% for JPT, followed by 400 mg/kg dose (57.63% for TSM and 64.79% for JPT) and then 200 mg/kg dose (52.35% for TSM and 54.46% for JPT). In addition, there were no significant differences (<i>p</i> < 0.05) in the RBC, MCV, and MCH levels of mice receiving JPT extract compared to the uninfected control. The WBC level of mice receiving 400 and 600 mg/kg of TSM, and 200 and 400 mg/kg of JPT, was significantly (<i>p</i> < 0.05) lower than the infected control, and the extracts did not significantly prevent the loss of platelets. For the acute toxicity test, there were no signs of toxicity or deaths in mice, and there were no differences in the histology, weight, or enzyme biochemistry of the liver and kidney between the extract and vehicle groups. However, the platelet count in the extract-treated mice was significantly higher than that in the control group. In conclusion, this study suggests that aqueous extracts of TSM and JPT have potent antimalarial activities and could be promising as new candidates for antimalarial drug development.</p>","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"2023 ","pages":"6624040"},"PeriodicalIF":2.8,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41098086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review on the Antimutagenic and Anticancer Effects of Cysteamine.","authors":"Chun-Man Lee","doi":"10.1155/2023/2419444","DOIUrl":"https://doi.org/10.1155/2023/2419444","url":null,"abstract":"<p><p>Cancer is one of the leading causes of death worldwide. First-line treatments usually include surgery, radiotherapy, and/or systemic therapy. These methods can be associated with serious adverse events and can be toxic to healthy cells. Despite the new advances in cancer therapies, there is still a continuous need for safe and effective therapeutic agents. Cysteamine is an aminothiol endogenously synthetized by human cells during the degradation of coenzyme-A. It has been safely used in humans for the treatment of several pathologies including cystinosis and neurodegenerative diseases. Cysteamine has been shown to be a potent antimutagenic, anticarcinogenic, and antimelanoma in various <i>in vitro</i> and <i>in vivo</i> studies, but a review on these aspects of cysteamine's use in medicine is lacking in the current literature. The efficacy of cysteamine has been shown <i>in vitro</i> and <i>in vivo</i> for the treatment of different types of cancer, such as gastrointestinal cancer, pancreatic cancer, sarcomas, hepatocellular carcinoma, and melanoma, leading to the significant reduction of lesions and/or the increase of survival time. Although the mechanisms of action are not fully understood, possible explanations are (i) free radical scavenging, (ii) alteration of the tumor cell proliferation by affecting nucleic acid and protein synthesis or inhibition of DNA synthesis, and (iii) hormone regulation. In conclusion, regarding the high safety profile of cysteamine and the current literature data presented in this article, cysteamine might be considered as an interesting molecule for the prevention and the treatment of cancer. Further clinical studies should be performed to support these data in humans.</p>","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"2023 ","pages":"2419444"},"PeriodicalIF":2.8,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41094793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ebselen, Iron Uptake Inhibitor, Alleviates Iron Overload-Induced Senescence-Like Neuronal Cells SH-SY5Y via Suppressing the mTORC1 Signaling Pathway.","authors":"Sirirak Mukem,&nbsp;Ibrahim Sayoh,&nbsp;Saowanee Maungchanburi,&nbsp;Tipsuda Thongbuakaew","doi":"10.1155/2023/6641347","DOIUrl":"https://doi.org/10.1155/2023/6641347","url":null,"abstract":"<p><p>Increasing evidence highlights that excessive iron accumulation in the brain plays a vital role in neuronal senescence and is implicated in the pathogenesis of age-related neurodegenerative diseases, including Alzheimer's disease (AD) and Parkinson's disease (PD). Therefore, the chemical compounds that eliminate an iron overload may provide better protection against oxidative stress conditions that cause the accumulation of senescent cells during brain aging. Ebselen has been identified as a strongly useful compound in the research on redox biology mechanisms. We hypothesized that ebselen could alleviate an iron overload-induced oxidative stress and consequently reverses the senescence-like phenotypes in the neuronal cells. In the present study, SH-SY5Y cells were treated with ferric ammonium citrate (FAC) before ebselen, and the evaluation of the cellular iron homeostasis, the indicators of oxidative stress, and the onset of senescence phenotypes and mechanisms were carried out accordingly. Our findings showed that ebselen ameliorated the FAC-mediated iron overload by decreasing the expression of divalent metal transporter 1 (DMT1) and ferritin light chain (FT-L) proteins. In contrast, it increased the expression of ferroportin 1 (FPN1) protein and its correlation led to a decrease in the expression of the cytosolic labile iron pool (LIP). Furthermore, ebselen significantly reduced reactive oxygen species (ROS) and rescued the mitochondrial membrane potential (ΔΨm). Notably, ebselen restored the biomarkers of cellular senescence by reducing the number of senescence-associated <i>β</i>-galactosidase (SA-<i>β</i>-gal) positive cells and senescence-associated secretory phenotypes (SASP). This also suppressed the expression of p53 protein targeting DNA damage response (DDR)/p21 cyclin-dependent kinase (CDK) inhibitor through a mTORC1 signaling pathway. Potentially, ebselen could be a therapeutic agent for treating brain aging and AD by mitigating iron accumulation and restoring senescence in SH-SY5Y cells.</p>","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"2023 ","pages":"6641347"},"PeriodicalIF":2.8,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10509000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41127490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Wound-Healing Activity of Hydrogel Extract of <i>Sansevieria trifasciata</i> Leaves (Asparagaceae).","authors":"Nia Yuniarsih,&nbsp;Himyatul Hidayah,&nbsp;Neni Sri Gunarti,&nbsp;Anggun Hari Kusumawati,&nbsp;Farhamzah Farhamzah,&nbsp;Asman Sadino,&nbsp;Maulana Yusuf Alkandahri","doi":"10.1155/2023/7680518","DOIUrl":"10.1155/2023/7680518","url":null,"abstract":"<p><p>For centuries, communities have used medicinal plants to treat various diseases, such as <i>Sansevieria trifasciata</i> (Asparagaceae), for wound healing. However, a study on the wound-healing activity of this plant has not been conducted. Therefore, this study aimed to evaluate the hydrogel formulations of <i>S. trifasciata</i> extract (HESt) and its activity in wound healing. The HESt formulations were subjected to physical examination, pH measurement, spreading coefficient, rheological study, stability test, and wound-healing activity. Furthermore, the HPMC and carbopol 940 gel-forming agents were used to obtain this formulation. In the incision wound model, the experiment was divided into 5 groups, each consisting of 4 mice. Groups 1 and 2 served as a negative and positive control (octenidine gel), while 3, 4, and 5 were given HESt formulations of 15%, 20%, and 25% (w/w), respectively, for 15 days. Based on the wound healing activity test, HESt 20% and 25% (w/w) groups showed significant (<i>p</i> <i><</i> 0.05) wound closure area on day 4 and from day 2 to 16. However, the HESt 15% (w/w) group showed no significant difference in wound-healing activity but had a higher closure than the negative control. Based on the evaluation of the hydrogel, all HESt formulations were reported to have fulfilled the standard requirements. The HESt formulations were also reported to be stable at various temperatures in the stability test. Therefore, <i>S. trifasciata</i> leaves extract has the potential to be developed as a wound-healing drug derived from herbal plants formulated into hydrogel preparations.</p>","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"2023 ","pages":"7680518"},"PeriodicalIF":2.8,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10480028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10179953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination of Chemically-Characterized Essential Oils from <i>Eucalyptus polybractea</i>, <i>Ormenis mixta,</i> and <i>Lavandula burnatii</i>: Optimization of a New Complete Antibacterial Formulation Using Simplex-Centroid Mixture Design.","authors":"Mohamed Jeddi,&nbsp;Naoufal El Hachlafi,&nbsp;Mouhcine Fadil,&nbsp;Nesrine Benkhaira,&nbsp;Samir Jeddi,&nbsp;Zineb Benziane Ouaritini,&nbsp;Kawtar Fikri-Benbrahim","doi":"10.1155/2023/5593350","DOIUrl":"10.1155/2023/5593350","url":null,"abstract":"<p><p>This study aims to identify the volatile profile of three essential oils obtained from <i>Eucalyptus polybractea cryptonifera</i> (EPEO), <i>Ormenis mixta</i> (OMEO), and <i>Lavandula burnatii briquet</i> (LBEO) and to examine their combined antibacterial activity that affords the optimal inhibitory ability against <i>S. aureus</i> and <i>E. coli</i> using simplex-centroid mixture design and checkerboard assay. Essential oils (EOs) were isolated by hydrodistillation and characterized using gas chromatography-mass spectrometry (GC-MS) and gas chromatography coupled with flame-ionization detector (GC-FID). The antibacterial activity was performed using disc diffusion and microdilution assays. The chemical analysis revealed that 1,8-cineole (23.75%), p-cymene (22.47%), and <i>α</i>-pinene (11.20%) and p-menthane-1,8-diol (18.19%), <i>α</i>-pinene (10.81%), and D-germacrene (9.17%) were the main components detected in <i>E. polybractea</i> and <i>O. mixta</i> EOs, respectively. However, <i>L. burnatii</i> EO was mainly represented by linalool (24.40%) and linalyl acetate (18.68%). The EPEO, LBEO, and OMEO had a strong antibacterial effect on <i>S. aureus</i> with minimal inhibitory concentrations (MICs) values ranging from 0.25 to 0.5% (v/v). Furthermore, the combination of 1/2048 MIC_EPEO + 1/4 MIC_LBEO showed a synergistic antibacterial effect on <i>S. aureus</i> with a FIC index of 0.25, while the formulation of 1/4 MIC_EPEO + 1/4 MIC_OMEO demonstrated an antibacterial synergistic activity on <i>E. coli</i> with a FIC index of 0.5. Moreover, the simplex-centroid mixture design reported that the most effective combinations on <i>E. coli</i> and <i>S. aureus</i> correspond to 32%/28%/40% and 35%/30%/35% of <i>E. polybractea</i>, <i>O. mixta,</i> and <i>L. burnatii,</i> respectively. Presented information highlights the action of antibacterial formulations of these EOs and suggests their potential applications as alternatives to commercialized drugs to contract the development of bacteria causing serious infections and food deterioration.</p>","PeriodicalId":7369,"journal":{"name":"Advances in Pharmacological and Pharmaceutical Sciences","volume":"2023 ","pages":"5593350"},"PeriodicalIF":2.8,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10120243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}