Victoire Nsabatien, Josue Zanga, Fiacre Agossa, Nono Mvuama, Maxwell Bamba, Osée Mansiangi, Leon Mbashi, Vanessa Mvudi, Glodie Diza, Dorcas Kantin, Narcisse Basosila, Hyacinthe Lukoki, Arsene Bokulu, Christelle Bosulu, Erick Bukaka, Jonas Nagahuedi, Jean Claude Palata, Emery Metelo
{"title":"Data from Entomological Collections of Aedes (Diptera: Culicidae) in a post-epidemic area of Chikungunya, City of Kinshasa, Democratic Republic of Congo","authors":"Victoire Nsabatien, Josue Zanga, Fiacre Agossa, Nono Mvuama, Maxwell Bamba, Osée Mansiangi, Leon Mbashi, Vanessa Mvudi, Glodie Diza, Dorcas Kantin, Narcisse Basosila, Hyacinthe Lukoki, Arsene Bokulu, Christelle Bosulu, Erick Bukaka, Jonas Nagahuedi, Jean Claude Palata, Emery Metelo","doi":"10.46471/gigabyte.96","DOIUrl":"https://doi.org/10.46471/gigabyte.96","url":null,"abstract":"Arbovirus epidemics (chikungunya, dengue, West Nile fever, yellow fever and zika) are a growing threat in African areas where Aedes (Stegomyia) aegypti (Linnaeus, 1762) and Aedes albopictus (Skuse, 1895) are present. The lack of comprehensive sampling of these two vectors limits our understanding of their propagation dynamics in areas at risk of arboviruses. Here, we collected 6,943 observations (both larval and human capture) of Ae. aegypti and Ae. albopictus between 2020 and 2022. The study was carried out in the Vallee de la Funa, a post-epidemic zone in the city of Kinshasa, Democratic Republic of Congo. Our results provide important information for future basic and advanced studies on the ecology and phenology of these vectors, as well as on vector dynamics after a post-epidemic period. The data from this study are published in the public domain as the Darwin Core Archive in the Global Biodiversity Information Facility.","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"8 14","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135391259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genome assembly and annotation of the Brown-Spotted Pit viper Protobothrops mucrosquamatus","authors":"Xiaotong Niu, Haorong Lu, Minhui Shi, Shiqing Wang, Yajie Zhou, Huan Liu","doi":"10.46471/gigabyte.97","DOIUrl":"https://doi.org/10.46471/gigabyte.97","url":null,"abstract":"The Brown-Spotted Pit viper (Protobothrops mucrosquamatus), also known as the Chinese habu, is a widespread and highly venomous snake distributed from Northeastern India to Eastern China. Genomics research can contribute to our understanding of venom components and natural selection in vipers. Here, we collected, sequenced and assembled the genome of a male P. mucrosquamatus individual from China. We generated a highly continuous reference genome, with a length of 1.53 Gb and 41.18% of repeat elements content. Using this genome, we identified 24,799 genes, 97.97% of which could be annotated. We verified the validity of our genome assembly and annotation process by generating a phylogenetic tree based on the nuclear genome single-copy genes of six other reptile species. The results of our research will contribute to future studies on Protobothrops biology and the genetic basis of snake venom.","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"28 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135480022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cristina Sánchez Gutierrez, Erika Santamaría, Carlos Andrés Morales, María Camila Lesmes, Horacio Cadena, Alvaro Avila-Diaz, Patricia Fuya, Catalina Marceló-Díaz
{"title":"Spatial patterns associated with the distribution of immature stages of <i>Aedes aegypti</i> in three dengue high-risk municipalities of Southwestern Colombia.","authors":"Cristina Sánchez Gutierrez, Erika Santamaría, Carlos Andrés Morales, María Camila Lesmes, Horacio Cadena, Alvaro Avila-Diaz, Patricia Fuya, Catalina Marceló-Díaz","doi":"10.46471/gigabyte.95","DOIUrl":"10.46471/gigabyte.95","url":null,"abstract":"<p><p><i>Aedes aegypti</i> mosquitoes are the main vector of human arbovirosis in tropical and subtropical areas. Their adaptation to urban and rural environments generates infestations inside households. Therefore, entomological surveillance associated with spatio-temporal analysis is an innovative approach for vector control and dengue management. Here, our main aim was to inspect immature pupal stages in households belonging to municipalities at high risk of dengue in Cauca, Colombia, by implementing entomological indices and relating how they influence adult mosquitos' density. We provide novel data for the geographical distribution of 3,806 immature pupal stages of <i>Ae. aegypti</i>. We also report entomological indices and spatial characterization. Our results suggest that, for <i>Ae. aegypti</i> species, pupal productivity generates high densities of adult mosquitos in neighbouring households, evidencing seasonal behaviour. Our dataset is essential as it provides an innovative strategy for mitigating vector-borne diseases using vector spatial patterns. It also delineates the association between these vector spatial patterns, entomological indicators, and breeding sites in high-risk neighbourhoods.</p>","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte95"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10620433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71489644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Robert E Bruccoleri, Edward J Oakeley, Ann Marie E Faust, Marc Altorfer, Sophie Dessus-Babus, David Burckhardt, Mevion Oertli, Ulrike Naumann, Frank Petersen, Joanne Wong
{"title":"Genome assembly of the bearded iris, <i>Iris pallida</i> Lam.","authors":"Robert E Bruccoleri, Edward J Oakeley, Ann Marie E Faust, Marc Altorfer, Sophie Dessus-Babus, David Burckhardt, Mevion Oertli, Ulrike Naumann, Frank Petersen, Joanne Wong","doi":"10.46471/gigabyte.94","DOIUrl":"10.46471/gigabyte.94","url":null,"abstract":"<p><p>Irises are perennial plants, representing a large genus with hundreds of species. While cultivated extensively for their ornamental value, commercial interest in irises lies in the secondary metabolites present in their rhizomes. The Dalmatian Iris (<i>Iris pallida</i> Lam.) is an ornamental plant that also produces secondary metabolites with potential value to the fragrance and pharmaceutical industries. In addition to providing base notes for the fragrance industry, iris tissues and extracts possess antioxidant, anti-inflammatory and immunomodulatory effects. However, study of these secondary metabolites has been hampered by a lack of genomic information, requiring difficult extraction and analysis techniques. Here, we report the genome sequence of <i>Iris pallida</i> Lam., generated with Pacific Bioscience long-read sequencing, resulting in a 10.04-Gbp assembly with a scaffold N50 of 14.34 Mbp and 91.8% complete BUSCOs. This reference genome will allow researchers to study the biosynthesis of these secondary metabolites in much greater detail, opening new avenues of investigation for drug discovery and fragrance formulations.</p>","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte94"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10565908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41222110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiangang Wang, Shiqing Wang, Song Huang, Qing Wang, Tianming Lan, Ming Jiang, Haitao Wu, Yuxiang Yuan
{"title":"The genome assembly and annotation of the Oriental rat snake <i>Ptyas mucosa</i>.","authors":"Jiangang Wang, Shiqing Wang, Song Huang, Qing Wang, Tianming Lan, Ming Jiang, Haitao Wu, Yuxiang Yuan","doi":"10.46471/gigabyte.92","DOIUrl":"https://doi.org/10.46471/gigabyte.92","url":null,"abstract":"<p><p>The Oriental rat snake <i>Ptyas mucosa</i> is a common non-venomous snake of the colubrid family, spanning most of South and Southeast Asia. <i>P. mucosa</i> is widely bred for its uses in traditional medicine, scientific research, and handicrafts. Therefore, genome resources of <i>P. mucosa</i> could play an important role in the efficacy of traditional medicine and the analysis of the living environment of this species. Here, we present a highly continuous <i>P. mucosa</i> genome with a size of 1.74 Gb. Its scaffold N50 length is 9.57 Mb, and the maximal scaffold length is 78.3 Mb. Its CG content is 37.9%, and its gene integrity reaches 86.6%. Assembled using long-reads, the total length of the repeat sequences in the genome reaches 735 Mb, and its repeat content is 42.19%. Finally, 24,869 functional genes were annotated in this genome. This study may assist in understanding <i>P. mucosa</i> and supporting medicinal research.</p>","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte92"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41171012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eamon Winden, Alejandro Vasquez-Echeverri, Susana Calle-Castañeda, Yumin Lian, Juan Pablo Hernandez Ortiz, David C Schwartz
{"title":"A database of restriction maps to expand the utility of bacterial artificial chromosomes.","authors":"Eamon Winden, Alejandro Vasquez-Echeverri, Susana Calle-Castañeda, Yumin Lian, Juan Pablo Hernandez Ortiz, David C Schwartz","doi":"10.46471/gigabyte.93","DOIUrl":"10.46471/gigabyte.93","url":null,"abstract":"<p><p>While Bacterial Artificial Chromosomes libraries were once a key resource for the genomic community, they have been obviated, for sequencing purposes, by long-read technologies. Such libraries may now serve as a valuable resource for manipulating and assembling large genomic constructs. To enhance accessibility and comparison, we have developed a BAC restriction map database. Using information from the National Center for Biotechnology Information's cloneDB FTP site, we constructed a database containing the restriction maps for both uniquely placed and insert-sequenced BACs from 11 libraries covering the recognition sequences of the available restriction enzymes. Along with the database, we generated a set of Python functions to reconstruct the database and more easily access the information within. This data is valuable for researchers simply using BACs, as well as those working with larger sections of the genome in terms of synthetic genes, large-scale editing, and mapping.</p>","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte93"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41164956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aine Fairbrother-Browne, Sonia García-Ruiz, Regina Hertfelder Reynolds, Mina Ryten, Alan Hodgkinson
{"title":"ensemblQueryR: fast, flexible and high-throughput querying of Ensembl LD API endpoints in R.","authors":"Aine Fairbrother-Browne, Sonia García-Ruiz, Regina Hertfelder Reynolds, Mina Ryten, Alan Hodgkinson","doi":"10.46471/gigabyte.91","DOIUrl":"10.46471/gigabyte.91","url":null,"abstract":"<p><p>We present ensemblQueryR, an R package for querying Ensembl linkage disequilibrium (LD) endpoints. This package is flexible, fast and user-friendly, and optimised for high-throughput querying. ensemblQueryR uses functions that are intuitive and amenable to custom code integration, familiar R object types as inputs and outputs as well as providing parallelisation functionality. For each Ensembl LD endpoint, ensemblQueryR provides two functions, permitting both single- and multi-query modes of operation. The multi-query functions are optimised for large query sizes and provide optional parallelisation to leverage available computational resources and minimise processing time. We demonstrate improved computational performance of ensemblQueryR over an exisiting tool in terms of random access memory (RAM) usage and speed, delivering a 10-fold speed increase whilst using a third of the RAM. Finally, ensemblQueryR is near-agnostic to operating system and computational architecture through Docker and singularity images, making this tool widely accessible to the scientific community.</p>","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41153439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xinyu Wang, Lirong Liu, Wenbiao Zhu, Shiqing Wang, Minhui Shi, Shuhui Yang, Haorong Lu, Jun Cao
{"title":"Genome assembly and annotation of the Sharp-nosed Pit Viper <i>Deinagkistrodon acutus</i> based on next-generation sequencing data.","authors":"Xinyu Wang, Lirong Liu, Wenbiao Zhu, Shiqing Wang, Minhui Shi, Shuhui Yang, Haorong Lu, Jun Cao","doi":"10.46471/gigabyte.88","DOIUrl":"10.46471/gigabyte.88","url":null,"abstract":"<p><p>The study of the currently known >3,000 species of snakes can provide valuable insights into the evolution of their genomes. <i>Deinagkistrodon acutus</i>, also known as Sharp-nosed Pit Viper, one hundred-pacer viper or five-pacer viper, is a venomous snake with significant economic, medicinal and scientific importance. Widely distributed in southeastern China and South-East Asia, <i>D. acutus</i> has been primarily studied for its venom. Here, we employed next-generation sequencing to assemble and annotate a highly continuous genome of <i>D. acutus</i>. The genome size is 1.46 Gb; its scaffold N50 length is 6.21 Mb, the repeat content is 42.81%, and 24,402 functional genes were annotated. This study helps to further understand and utilize <i>D. acutus</i> and its venom at the genetic level.</p>","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte88"},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10498098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10268545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lucia Corte, Lathan Liou, Paul F O'Reilly, Judit García-González
{"title":"Trumpet plots: visualizing the relationship between allele frequency and effect size in genetic association studies.","authors":"Lucia Corte, Lathan Liou, Paul F O'Reilly, Judit García-González","doi":"10.46471/gigabyte.89","DOIUrl":"10.46471/gigabyte.89","url":null,"abstract":"<p><p>Recent advances in genome-wide association and sequencing studies have shown that the genetic architecture of complex traits and diseases involves a combination of rare and common genetic variants distributed throughout the genome. One way to better understand this architecture is to visualize genetic associations across a wide range of allele frequencies. However, there is currently no standardized or consistent graphical representation for effectively illustrating these results. Here we propose a standardized approach for visualizing the effect size of risk variants across the allele frequency spectrum. The proposed plots have a distinctive trumpet shape: with the majority of variants having high frequency and small effects, and a small number of variants having lower frequency and larger effects. To demonstrate the utility of trumpet plots in illustrating the relationship between the number of variants, their frequency, and the magnitude of their effects in shaping the genetic architecture of complex traits and diseases, we generated trumpet plots for more than one hundred traits in the UK Biobank. To facilitate their broader use, we developed an R package, 'TrumpetPlots' (available at the Comprehensive R Archive Network) and R Shiny application, 'Shiny Trumpets' (available at https://juditgg.shinyapps.io/shinytrumpets/) that allows users to explore these results and submit their own data.</p>","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte89"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10498096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10268544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sonia García-Ruiz, Regina Hertfelder Reynolds, Melissa Grant-Peters, Emil Karl Gustavsson, Aine Fairbrother-Browne, Zhongbo Chen, Jonathan William Brenton, Mina Ryten
{"title":"aws-s3-integrity-check: an open-source bash tool to verify the integrity of a dataset stored on Amazon S3.","authors":"Sonia García-Ruiz, Regina Hertfelder Reynolds, Melissa Grant-Peters, Emil Karl Gustavsson, Aine Fairbrother-Browne, Zhongbo Chen, Jonathan William Brenton, Mina Ryten","doi":"10.46471/gigabyte.87","DOIUrl":"10.46471/gigabyte.87","url":null,"abstract":"<p><p>Amazon Simple Storage Service (Amazon S3) is a widely used platform for storing large biomedical datasets. Unintended data alterations can occur during data writing and transmission, altering the original content and generating unexpected results. However, no open-source and easy-to-use tool exists to verify end-to-end data integrity. Here, we present <i>aws-s3-integrity-check</i>, a user-friendly, lightweight, and reliable bash tool to verify the integrity of a dataset stored in an Amazon S3 bucket. Using this tool, we only needed ∼114 min to verify the integrity of 1,045 records ranging between 5 bytes and 10 gigabytes and occupying ∼935 gigabytes of the Amazon S3 cloud. Our <i>aws-s3-integrity-check</i> tool also provides file-by-file on-screen and log-file-based information about the status of each integrity check. To our knowledge, this tool is the only open-source one that allows verifying the integrity of a dataset uploaded to the Amazon S3 Storage quickly, reliably, and efficiently. The tool is freely available for download and use at https://github.com/SoniaRuiz/aws-s3-integrity-check and https://hub.docker.com/r/soniaruiz/aws-s3-integrity-check.</p>","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte87"},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10165035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}