Gene and genome editing最新文献_第3页

Genome editing technology and applications with the type I CRISPR system 基因组编辑技术及其与I型CRISPR系统的应用

Gene and genome editing Pub Date : 2022-12-01 DOI: 10.1016/j.ggedit.2022.100013

Kazuto Yoshimi , Tomoji Mashimo

{"title":"Genome editing technology and applications with the type I CRISPR system","authors":"Kazuto Yoshimi , Tomoji Mashimo","doi":"10.1016/j.ggedit.2022.100013","DOIUrl":"10.1016/j.ggedit.2022.100013","url":null,"abstract":"<div><p>Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems, which are representative genome editing technologies, are classified into class 1 and class 2 in terms of evolutionary biology and are further classified into several subtypes. Class 2 CRISPR systems, including type II Cas9 and type V Cas12a, are the most commonly used for genome editing in eukaryotic cells, while type I CRISPR systems within Class 1 are also becoming available. Type I CRISPR recognizes longer target sequences than CRISPR-Cas9 and can induce large deletion mutations of several kilobases. These features demonstrate its potential as a novel and unique genome editing tool that can induce genetic disruption safely and reliably. Thus, it is expected to be utilized for gene therapy and industrial applications. Recently, the DNA cleavage mechanism of type I CRISPR has also revealed details from protein-complex analyses with X-ray crystallography, cryo-electron microscopy, and high-speed atomic force microscopy. The single-strand DNA trans-cleavage activity of type I CRISPR, called collateral activity, has broadened the potential application for CRISPR diagnostics, especially in the development of point-of-care testing methods for COVID-19. In this review, we present an overview of the type I CRISPR system, its application to genome editing, and genetic diagnosis using CRISPR-Cas3.</p></div>","PeriodicalId":73137,"journal":{"name":"Gene and genome editing","volume":"3 ","pages":"Article 100013"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266638802200003X/pdfft?md5=8eab8b309522c5e7bbddba69bc0770e0&pid=1-s2.0-S266638802200003X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43940303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}