{"title":"小鼠基因组编辑及其在精子发生研究中的应用","authors":"Seiya Oura , Hideto Mori , Masahito Ikawa","doi":"10.1016/j.ggedit.2022.100014","DOIUrl":null,"url":null,"abstract":"<div><p>Gene modification technology has long been beneficial for unraveling the mystery of biological phenomena. Thus, the advent of the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated (Cas) 9 system was a game-changer in biological research because of its efficiency and simplicity of design. This review article first describes a brief guideline of gRNA design and the methods to incorporate the CRISPR/Cas9 system into zygotes. Then, we will also discuss the application of this technology to the study of male reproductive biology, including knock-out (KO) phenotypical screening of genes expressed in the male reproductive tissues.</p></div>","PeriodicalId":73137,"journal":{"name":"Gene and genome editing","volume":"3 ","pages":"Article 100014"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666388022000041/pdfft?md5=c8b36d0f294a2356a30167622ac7c601&pid=1-s2.0-S2666388022000041-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Genome editing in mice and its application to the study of spermatogenesis\",\"authors\":\"Seiya Oura , Hideto Mori , Masahito Ikawa\",\"doi\":\"10.1016/j.ggedit.2022.100014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Gene modification technology has long been beneficial for unraveling the mystery of biological phenomena. Thus, the advent of the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated (Cas) 9 system was a game-changer in biological research because of its efficiency and simplicity of design. This review article first describes a brief guideline of gRNA design and the methods to incorporate the CRISPR/Cas9 system into zygotes. Then, we will also discuss the application of this technology to the study of male reproductive biology, including knock-out (KO) phenotypical screening of genes expressed in the male reproductive tissues.</p></div>\",\"PeriodicalId\":73137,\"journal\":{\"name\":\"Gene and genome editing\",\"volume\":\"3 \",\"pages\":\"Article 100014\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2666388022000041/pdfft?md5=c8b36d0f294a2356a30167622ac7c601&pid=1-s2.0-S2666388022000041-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene and genome editing\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666388022000041\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene and genome editing","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666388022000041","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Genome editing in mice and its application to the study of spermatogenesis
Gene modification technology has long been beneficial for unraveling the mystery of biological phenomena. Thus, the advent of the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated (Cas) 9 system was a game-changer in biological research because of its efficiency and simplicity of design. This review article first describes a brief guideline of gRNA design and the methods to incorporate the CRISPR/Cas9 system into zygotes. Then, we will also discuss the application of this technology to the study of male reproductive biology, including knock-out (KO) phenotypical screening of genes expressed in the male reproductive tissues.
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