GEN biotechnology最新文献_第4页

A Prime Example of Dual Deception 双重欺骗的典型例子

GEN biotechnology Pub Date : 2023-08-01 DOI: 10.1089/genbio.2023.0036

M. A. Mahmood, Shahida Mansoor

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Fanzor: First CRISPR-Like System Found in Eukaryotes Fanzor:在真核生物中发现的第一个crispr样系统

GEN biotechnology Pub Date : 2023-08-01 DOI: 10.1089/genbio.2023.29110.gsw

{"title":"Fanzor: First CRISPR-Like System Found in Eukaryotes","authors":"","doi":"10.1089/genbio.2023.29110.gsw","DOIUrl":"https://doi.org/10.1089/genbio.2023.29110.gsw","url":null,"abstract":"GEN BiotechnologyVol. 2, No. 4 News FeaturesFree AccessFanzor: First CRISPR-Like System Found in EukaryotesGEN Staff WriterGEN Staff WriterPublished Online:17 Aug 2023https://doi.org/10.1089/genbio.2023.29110.gswAboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail Feng Zhang's team at the Broad Institute discovers RNA-guided Fanzor enzymes from fungi and amoeba that show promise for genome editing applications.A cryo-EM map of a Fanzor protein (gray, yellow, light blue, and pink) in complex with ωRNA (purple) and its target DNA (red). Non-target DNA strand in blue.(Credit: Zhang lab, Broad Institute of MIT and Harvard/McGovern Institute for Brain Research at MIT).Another potentially game-changing discovery in the world of genome editing has come out of the Broad Institute lab of Feng Zhang. In a study published in Nature, Zhang and colleagues have uncovered the first programmable RNA-guided system in eukaryotes.1 Earlier this year, the Zhang lab adapted a contractile injection system, found naturally in bacteria, that delivers protein payloads to target human cells.2In the latest study, Zhang's team describes how the eukaryotic system—based on the Fanzor protein—uses RNA as a guide to target DNA precisely and shows that Fanzor can be reprogrammed to edit the genome of human cells. In addition, the compact Fanzor systems have the potential to be more easily delivered to cells and tissues as therapeutics than CRISPR-Cas systems. Further refinements to improve their targeting efficiency could make them a valuable new technology for human genome editing.Scientists have long wondered whether systems similar to the well-known bacterial CRISPR system exist in eukaryotes. In a recent analysis published in The CRISPR Journal (a sister journal to GEN Biotechnology), Blake Wiedenheft's group at Montana State University studied repetitive elements that had previously been identified as putative CRISPR loci in the human genome. They showed that these repeats do not contain the repeat-spacer-repeat architecture nor the cas nuclease genes characteristic of functional CRISPR systems.3“Overall, the evidence for functional CRISPR-Cas systems in the human genome and other eukaryotic genomes remains unsupported,” the authors wrote. “Although the phylogenetic and functional diversity of CRISPRs continue to expand, (to date) CRISPR-Cas adaptive immune systems remain restricted to prokaryotes.”3Present and CorrectNevertheless, the new Zhang lab study demonstrates that RNA-guided DNA-cutting mechanisms are present across all kingdoms of life.“CRISPR-based systems are widely used and powerful because they can be easily reprogrammed to target different sites in the genome,” said Zhang, a core institute member at the Broad Institute. “This new system is another way to make precise changes in human cells, complementing the genome editing tools we already","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136221685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR-Cas9-Mediated Mutagenesis of the Asian Citrus Psyllid, Diaphorina citri crispr - cas9介导的亚洲柑橘木虱的诱变

GEN biotechnology Pub Date : 2023-08-01 DOI: 10.1089/genbio.2023.0022

Duverney Chaverra-Rodriguez, Michelle Bui, Cody L. Gilleland, Jason L. Rasgon, Omar S. Akbari

{"title":"CRISPR-Cas9-Mediated Mutagenesis of the Asian Citrus Psyllid, <i>Diaphorina citri</i>","authors":"Duverney Chaverra-Rodriguez, Michelle Bui, Cody L. Gilleland, Jason L. Rasgon, Omar S. Akbari","doi":"10.1089/genbio.2023.0022","DOIUrl":"https://doi.org/10.1089/genbio.2023.0022","url":null,"abstract":"The most devastating disease affecting the global citrus industry is Huanglongbing (HLB), caused by the pathogen Candidatus Liberibacter asiaticus. HLB is primarily spread by the insect vector Diaphorina citri (Asian Citrus Psyllid). To counteract the rapid spread of HLB by D. citri, traditional vector control strategies such as insecticide sprays, the release of natural predators, and mass introductions of natural parasitoids are used. However, these methods alone have not managed to contain the spread of disease. To further expand the available tools for D. citri control through generating specific modifications of the D. citri genome, we have developed protocols for CRISPR-Cas9-based genetic modification. Until now, genome editing in D. citri has been challenging due to the general fragility and size of D. citri eggs. Here we present optimized methods for collecting and preparing eggs to introduce the Cas9 ribonucleoprotein (RNP) into early embryos and alternative methods of injecting RNP into the hemocoel of adult females for ovarian transduction. Using these methods, we have generated visible somatic mutations, indicating their suitability for gene editing in D. citri. These methods represent the first steps toward advancing D. citri research in preparation for future genetic-based systems for controlling HLB.","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136221684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Creating Self-Organizing Systems with Synthetic Multicellularity 用合成多细胞创造自组织系统

GEN biotechnology Pub Date : 2023-08-01 DOI: 10.1089/genbio.2023.29112.sro

Sachin Rawat

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From the Boardroom to BIO: An Interview with Rachel King 从董事会会议室到BIO：Rachel King访谈录

GEN biotechnology Pub Date : 2023-08-01 DOI: 10.1089/genbio.2023.29104.rki

Rachel King, A. Philippidis

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Seeing the Action of Lipid Droplets in Aging and Longevity 脂滴在衰老和长寿中的作用

GEN biotechnology Pub Date : 2023-08-01 DOI: 10.1089/genbio.2023.29106.bta

Bo Tao, Hongyuan Yang, Rong Fan