{"title":"Simple Low-Cost Production of DNA MS2 Virus-Like Particles As Molecular Diagnostic Controls.","authors":"Michael A Crone, Paul S Freemont","doi":"10.1089/genbio.2022.0033","DOIUrl":"https://doi.org/10.1089/genbio.2022.0033","url":null,"abstract":"<p><p>Suitable controls are integral for the validation and continued quality assurance of diagnostic workflows. Plasmids, DNA, or <i>in vitro</i> transcribed RNA are often used to validate novel diagnostic workflows, however, they are poorly representative of clinical samples. RNA phage virus-like particles (VLPs) packaged with exogenous RNA have been used in clinical diagnostics as workflow controls, serving as surrogates for infectious viral particles. Comparable controls for DNA viruses are more challenging to produce, with analogous DNA phages being infectious and packaging of DNA within RNA phages requiring complex purification procedures and expensive chemical linkers. We present a simple and inexpensive method to produce <i>Emesvirus zinderi</i> (MS2) VLPs, packaged with DNA, that makes use of affinity chromatography for purification and enzymatic production of exogenous DNA suitable for packaging. The produced VLPs were packaged with hepatitis B virus DNA and were then quantified using droplet digital PCR and calibrated against the WHO international standard using a commercial assay in an accredited clinical laboratory.</p>","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"1 6","pages":"496-503"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10590110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GEN biotechnologyPub Date : 2022-12-01DOI: 10.1089/genbio.2022.29066.jle
Julianna LeMieux
{"title":"PacBio Seeks to Maroon the Competition in the Crowded Sequencing Space","authors":"Julianna LeMieux","doi":"10.1089/genbio.2022.29066.jle","DOIUrl":"https://doi.org/10.1089/genbio.2022.29066.jle","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44200846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GEN biotechnologyPub Date : 2022-12-01DOI: 10.1089/genbio.2022.29071.editorial
Hana El-Samad
{"title":"We Don't Need No Education Misinformation","authors":"Hana El-Samad","doi":"10.1089/genbio.2022.29071.editorial","DOIUrl":"https://doi.org/10.1089/genbio.2022.29071.editorial","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44143881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GEN biotechnologyPub Date : 2022-12-01DOI: 10.1089/genbio.2022.29064.jdg
Jonathan D. Grinstein
{"title":"Capturing Carbon with CRISPR Crops","authors":"Jonathan D. Grinstein","doi":"10.1089/genbio.2022.29064.jdg","DOIUrl":"https://doi.org/10.1089/genbio.2022.29064.jdg","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41898627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GEN biotechnologyPub Date : 2022-10-01DOI: 10.1089/genbio.2022.29059.jdo
J. Doudna, Julianna LeMieux
{"title":"CRISPR's Second Decade: Jennifer Doudna Looks Forward and Back","authors":"J. Doudna, Julianna LeMieux","doi":"10.1089/genbio.2022.29059.jdo","DOIUrl":"https://doi.org/10.1089/genbio.2022.29059.jdo","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42502563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GEN biotechnologyPub Date : 2022-10-01DOI: 10.1089/genbio.2022.29062.jqu
J. Quackenbush
{"title":"Counting Genes: A Human Genome Conundrum","authors":"J. Quackenbush","doi":"10.1089/genbio.2022.29062.jqu","DOIUrl":"https://doi.org/10.1089/genbio.2022.29062.jqu","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45655614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}