GEN biotechnology最新文献

筛选
英文 中文
Simple Low-Cost Production of DNA MS2 Virus-Like Particles As Molecular Diagnostic Controls. 简单低成本生产DNA MS2病毒样颗粒作为分子诊断控制。
GEN biotechnology Pub Date : 2022-12-01 DOI: 10.1089/genbio.2022.0033
Michael A Crone, Paul S Freemont
{"title":"Simple Low-Cost Production of DNA MS2 Virus-Like Particles As Molecular Diagnostic Controls.","authors":"Michael A Crone,&nbsp;Paul S Freemont","doi":"10.1089/genbio.2022.0033","DOIUrl":"https://doi.org/10.1089/genbio.2022.0033","url":null,"abstract":"<p><p>Suitable controls are integral for the validation and continued quality assurance of diagnostic workflows. Plasmids, DNA, or <i>in vitro</i> transcribed RNA are often used to validate novel diagnostic workflows, however, they are poorly representative of clinical samples. RNA phage virus-like particles (VLPs) packaged with exogenous RNA have been used in clinical diagnostics as workflow controls, serving as surrogates for infectious viral particles. Comparable controls for DNA viruses are more challenging to produce, with analogous DNA phages being infectious and packaging of DNA within RNA phages requiring complex purification procedures and expensive chemical linkers. We present a simple and inexpensive method to produce <i>Emesvirus zinderi</i> (MS2) VLPs, packaged with DNA, that makes use of affinity chromatography for purification and enzymatic production of exogenous DNA suitable for packaging. The produced VLPs were packaged with hepatitis B virus DNA and were then quantified using droplet digital PCR and calibrated against the WHO international standard using a commercial assay in an accredited clinical laboratory.</p>","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"1 6","pages":"496-503"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9814128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10590110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Made in Britain: Venture Fund Boosts Synthetic Biology 英国制造:风险基金促进合成生物学
GEN biotechnology Pub Date : 2022-12-01 DOI: 10.1089/genbio.2022.29068.aph
A. Philippidis
{"title":"Made in Britain: Venture Fund Boosts Synthetic Biology","authors":"A. Philippidis","doi":"10.1089/genbio.2022.29068.aph","DOIUrl":"https://doi.org/10.1089/genbio.2022.29068.aph","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42358706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vanquishing the Virus 战胜病毒
GEN biotechnology Pub Date : 2022-12-01 DOI: 10.1089/genbio.2022.29074.jle
Julianna LeMieux
{"title":"Vanquishing the Virus","authors":"Julianna LeMieux","doi":"10.1089/genbio.2022.29074.jle","DOIUrl":"https://doi.org/10.1089/genbio.2022.29074.jle","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41434503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PacBio Seeks to Maroon the Competition in the Crowded Sequencing Space PacBio寻求在拥挤的测序空间中竞争
GEN biotechnology Pub Date : 2022-12-01 DOI: 10.1089/genbio.2022.29066.jle
Julianna LeMieux
{"title":"PacBio Seeks to Maroon the Competition in the Crowded Sequencing Space","authors":"Julianna LeMieux","doi":"10.1089/genbio.2022.29066.jle","DOIUrl":"https://doi.org/10.1089/genbio.2022.29066.jle","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44200846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
We Don't Need No Education Misinformation 我们不需要教育错误信息
GEN biotechnology Pub Date : 2022-12-01 DOI: 10.1089/genbio.2022.29071.editorial
Hana El-Samad
{"title":"We Don't Need No Education Misinformation","authors":"Hana El-Samad","doi":"10.1089/genbio.2022.29071.editorial","DOIUrl":"https://doi.org/10.1089/genbio.2022.29071.editorial","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44143881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Capturing Carbon with CRISPR Crops 利用CRISPR作物捕获碳
GEN biotechnology Pub Date : 2022-12-01 DOI: 10.1089/genbio.2022.29064.jdg
Jonathan D. Grinstein
{"title":"Capturing Carbon with CRISPR Crops","authors":"Jonathan D. Grinstein","doi":"10.1089/genbio.2022.29064.jdg","DOIUrl":"https://doi.org/10.1089/genbio.2022.29064.jdg","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41898627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CRISPR's Second Decade: Jennifer Doudna Looks Forward and Back CRISPR的第二个十年:Jennifer Doudna展望未来
GEN biotechnology Pub Date : 2022-10-01 DOI: 10.1089/genbio.2022.29059.jdo
J. Doudna, Julianna LeMieux
{"title":"CRISPR's Second Decade: Jennifer Doudna Looks Forward and Back","authors":"J. Doudna, Julianna LeMieux","doi":"10.1089/genbio.2022.29059.jdo","DOIUrl":"https://doi.org/10.1089/genbio.2022.29059.jdo","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42502563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Billionaire's Guide to Pandemic Prevention 《亿万富翁流行病预防指南
GEN biotechnology Pub Date : 2022-10-01 DOI: 10.1089/genbio.2022.29051.sek
S. Ekins
{"title":"The Billionaire's Guide to Pandemic Prevention","authors":"S. Ekins","doi":"10.1089/genbio.2022.29051.sek","DOIUrl":"https://doi.org/10.1089/genbio.2022.29051.sek","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43800215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The View from ARK Invest ARK Invest的观点
GEN biotechnology Pub Date : 2022-10-01 DOI: 10.1089/genbio.2022.29061.sba
S. Barnett, Ali Urman
{"title":"The View from ARK Invest","authors":"S. Barnett, Ali Urman","doi":"10.1089/genbio.2022.29061.sba","DOIUrl":"https://doi.org/10.1089/genbio.2022.29061.sba","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44701310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Counting Genes: A Human Genome Conundrum 基因计数:人类基因组难题
GEN biotechnology Pub Date : 2022-10-01 DOI: 10.1089/genbio.2022.29062.jqu
J. Quackenbush
{"title":"Counting Genes: A Human Genome Conundrum","authors":"J. Quackenbush","doi":"10.1089/genbio.2022.29062.jqu","DOIUrl":"https://doi.org/10.1089/genbio.2022.29062.jqu","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45655614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信
小红书