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Real-Time Monitoring in Biomanufacturing with Graphene Field-Effect Transistor Sensors: Detection of pH, Glucose, and Antibodies. 石墨烯场效应晶体管传感器在生物制造中的实时监测：pH值、葡萄糖和抗体的检测。
IF 2
GEN biotechnology Pub Date : 2024-12-13 eCollection Date: 2024-12-01 DOI: 10.1089/genbio.2024.0043
Deependra K Ban, Reza Hajian, Matthew Chan, Shiva Abdolrahimi, Francie Barron, Saurav Datta, Kiana Aran
{"title":"Real-Time Monitoring in Biomanufacturing with Graphene Field-Effect Transistor Sensors: Detection of pH, Glucose, and Antibodies.","authors":"Deependra K Ban, Reza Hajian, Matthew Chan, Shiva Abdolrahimi, Francie Barron, Saurav Datta, Kiana Aran","doi":"10.1089/genbio.2024.0043","DOIUrl":"10.1089/genbio.2024.0043","url":null,"abstract":"<p><p>In high-efficiency smart biomanufacturing, continuous monitoring of products, byproducts, and reagents is crucial for optimizing production processes, improving yield, and ensuring product quality. This monitoring maintains optimal conditions, reduces waste, and enables swift corrective actions, minimizing the risk of producing out-of-specification products. With advancements in cell-free biomanufacturing, the importance of in-line sensing technologies has increased, as they provide real-time tracking of biochemical processes, allowing immediate adjustments to keep manufacturing efficient and consistent. In this study, we successfully developed a graphene field-effect transistor (gFET) sensor to monitor key parameters such as glucose, pH, and immunoglobulin G antibody levels in cell culture media from a CHO cells bioreactor. The gFET sensor accurately detected pH levels between 6.8 and 8.2, glucose concentrations from 5 to 30 mM, and antibody levels ranging from 25 to 100 µg/mL, highlighting the potential of graphene sensors for inline sensing in advanced biomanufacturing.</p>","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"3 6","pages":"341-349"},"PeriodicalIF":2.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Revolutionizing Postdoctoral Training Using the Social Ecological Model: Insights and Experiences from the Propel Scholars. 利用社会生态模式革新博士后培养：推进学者的见解与经验。
IF 2
GEN biotechnology Pub Date : 2024-08-01 Epub Date: 2024-08-16 DOI: 10.1089/genbio.2024.0014
Cellas A Hayes, Colwyn A Headley, Amy R Nava, Edward A Vizcarra, Keith C Garcia, McKay M S Mullen, Josefina Flores Morales, Anthony C Venida, Shawna Follis
{"title":"Revolutionizing Postdoctoral Training Using the Social Ecological Model: Insights and Experiences from the Propel Scholars.","authors":"Cellas A Hayes, Colwyn A Headley, Amy R Nava, Edward A Vizcarra, Keith C Garcia, McKay M S Mullen, Josefina Flores Morales, Anthony C Venida, Shawna Follis","doi":"10.1089/genbio.2024.0014","DOIUrl":"https://doi.org/10.1089/genbio.2024.0014","url":null,"abstract":"<p><p>The dissatisfaction within the postdoctoral training phase has led to the drastic reduction in the number of U.S. citizens pursuing postdoctoral positions within the biological and biomedical sciences fields. Even more so, there is an obvious disparity in not only the recruitment but the retention among underrepresented groups to pursue careers as academic scientists. The proposed social-ecological model and National Institute of Health advisory committee suggests reforming the postdoctoral training phase to overcome these downward trends and disparities. Importantly, some programs like the Stanford Propel Postdoctoral Program were integrating this framework and recommendations without knowledge that they would be released 2 years later. The goal of the Propel Program is to provide social, cohort, financial, and institutional support to diverse cohorts of postdoctoral trainee to diversify the professoriate. Within this piece, several of the Propel scholars come together to provide their perspectives on how the Propel Program has benefited their postdoctoral training experience.</p>","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"3 4","pages":"196-206"},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12288851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144710065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Fluorescent Sex-Sorting Technique for Insects with the Demonstration in Drosophila melanogaster. 以黑腹果蝇为示范的昆虫荧光性别分类技术。
IF 2
GEN biotechnology Pub Date : 2024-02-01 Epub Date: 2024-02-15 DOI: 10.1089/genbio.2023.0041
Junru Liu, Danny Rayes, Omar S Akbari
{"title":"A Fluorescent Sex-Sorting Technique for Insects with the Demonstration in <i>Drosophila melanogaster</i>.","authors":"Junru Liu, Danny Rayes, Omar S Akbari","doi":"10.1089/genbio.2023.0041","DOIUrl":"10.1089/genbio.2023.0041","url":null,"abstract":"<p><p>Recent advances in insect genetic engineering offer alternative genetic biocontrol solutions to control populations of pests and disease vectors. While success has been achieved, sex-sorting remains problematic for scaling many genetic biocontrol interventions. Here, we describe the development of a genetically stable sex-sorting technique for female and male selection with a proof of concept in <i>Drosophila melanogaster</i> termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter). This elegant approach utilizes dominantly expressed fluorescent proteins and differentially spliced introns to ensure sex-specific expression. The system has the potential for adaptability to various insect species and application for high-throughput insect sex-sorting.</p>","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"3 1","pages":"35-44"},"PeriodicalIF":2.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10895710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139984733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Physical View of the Origin of Genetic Engineering 基因工程起源的物理学观点
GEN biotechnology Pub Date : 2023-12-07 DOI: 10.1089/genbio.2023.0037
Anna Yakimova, Denis Baranovskii, Sergei A. Ivanov, Peter Shegay, A. D. Kaprin, Ilya D. Klabukov
{"title":"The Physical View of the Origin of Genetic Engineering","authors":"Anna Yakimova, Denis Baranovskii, Sergei A. Ivanov, Peter Shegay, A. D. Kaprin, Ilya D. Klabukov","doi":"10.1089/genbio.2023.0037","DOIUrl":"https://doi.org/10.1089/genbio.2023.0037","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"17 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138591361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vivo Prime Editing by Lipid Nanoparticle Co-Delivery of Chemically Modified pegRNA and Prime Editor mRNA 通过脂质纳米颗粒共同递送化学修饰的 pegRNA 和 Prime Editor mRNA 进行体内 Prime 编辑
GEN biotechnology Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.0045
Zexiang Chen, Karen Kelly, Hao-Feng Cheng, Xiaolong Dong, Adam K. Hedger, Li Li, E. Sontheimer, Jonathan K. Watts
{"title":"In Vivo Prime Editing by Lipid Nanoparticle Co-Delivery of Chemically Modified pegRNA and Prime Editor mRNA","authors":"Zexiang Chen, Karen Kelly, Hao-Feng Cheng, Xiaolong Dong, Adam K. Hedger, Li Li, E. Sontheimer, Jonathan K. Watts","doi":"10.1089/genbio.2023.0045","DOIUrl":"https://doi.org/10.1089/genbio.2023.0045","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"177 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139023046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Point and Click: DNA Polymerase Prime Editors Upgrade Gene Rewriting Arsenal 指点江山DNA 聚合酶主编辑器升级基因改写武器库
GEN biotechnology Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.29120.jdg
Jonathan D. Grinstein
{"title":"Point and Click: DNA Polymerase Prime Editors Upgrade Gene Rewriting Arsenal","authors":"Jonathan D. Grinstein","doi":"10.1089/genbio.2023.29120.jdg","DOIUrl":"https://doi.org/10.1089/genbio.2023.29120.jdg","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"213 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139020034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved Cell-Type Identification and Comprehensive Mapping of Regulatory Features with Spatial Epigenomics 96-Channel Microfluidic Platform 利用空间表观组学 96 通道微流控平台改进细胞类型鉴定并全面绘制调控特征图
GEN biotechnology Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.0044
Zev Kartiganer, Gumaro Rojas, Machele Riccio, Abigail Tyree, Katelyn Noronha, Molly Wetzel, Joshua Barnett, James McGann, Jennifer Garbarino, Daniel Massucci, Nouroddin Sotoudeh Chafi, Silas Decker, Arianna McDaniels, Jeffrey Sabina, David Levchenko, Jose Perez, Colin Ng, Kenneth Wang
{"title":"Improved Cell-Type Identification and Comprehensive Mapping of Regulatory Features with Spatial Epigenomics 96-Channel Microfluidic Platform","authors":"Zev Kartiganer, Gumaro Rojas, Machele Riccio, Abigail Tyree, Katelyn Noronha, Molly Wetzel, Joshua Barnett, James McGann, Jennifer Garbarino, Daniel Massucci, Nouroddin Sotoudeh Chafi, Silas Decker, Arianna McDaniels, Jeffrey Sabina, David Levchenko, Jose Perez, Colin Ng, Kenneth Wang","doi":"10.1089/genbio.2023.0044","DOIUrl":"https://doi.org/10.1089/genbio.2023.0044","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"194 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139021568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Defining the Rules of Biology in the Age of Genome Writing 在基因组写作时代定义生物学规则
GEN biotechnology Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.29125.awo
A. Woolfson
{"title":"Defining the Rules of Biology in the Age of Genome Writing","authors":"A. Woolfson","doi":"10.1089/genbio.2023.29125.awo","DOIUrl":"https://doi.org/10.1089/genbio.2023.29125.awo","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"894 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139022633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Drug Pricing Versus Precision Medicine 药物定价与精准医疗
GEN biotechnology Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.29124.kda
Kevin Davies
{"title":"Drug Pricing Versus Precision Medicine","authors":"Kevin Davies","doi":"10.1089/genbio.2023.29124.kda","DOIUrl":"https://doi.org/10.1089/genbio.2023.29124.kda","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"129 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138988989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Revelry and Reminiscence: 50 Years of Recombinant DNA at Cold Spring Harbor Laboratory 狂欢与回忆：冷泉港实验室 DNA 重组 50 周年
GEN biotechnology Pub Date : 2023-12-01 DOI: 10.1089/genbio.2023.29122.fli
Fay Lin
{"title":"Revelry and Reminiscence: 50 Years of Recombinant DNA at Cold Spring Harbor Laboratory","authors":"Fay Lin","doi":"10.1089/genbio.2023.29122.fli","DOIUrl":"https://doi.org/10.1089/genbio.2023.29122.fli","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"65 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139013095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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