Acta Crystallographica Section F-structural Biology and Crystallization Communications最新文献_第7页

Structural insights into ChpT, an essential dimeric histidine phosphotransferase regulating the cell cycle in Caulobacter crescentus. 新月杆菌中调控细胞周期的重要二聚组氨酸磷酸转移酶 ChpT 的结构研究。

IF 0.9 4区生物学

Acta Crystallographica Section F-structural Biology and Crystallization Communications Pub Date : 2012-09-01 Epub Date: 2012-08-29 DOI: 10.1107/S1744309112033064

Antonella Fioravanti, Bernard Clantin, Frédérique Dewitte, Zoé Lens, Alexis Verger, Emanuele G Biondi, Vincent Villeret

引用次数: 0

Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus. 美洲小龙虾重组甲壳蛋白亚基的结构特征。

IF 0.9 4区生物学

Acta Crystallographica Section F-structural Biology and Crystallization Communications Pub Date : 2012-08-01 Epub Date: 2012-07-26 DOI: 10.1107/S1744309112026103

Michele Ferrari, Claudia Folli, Elisa Pincolini, Timothy S McClintock, Manfred Rössle, Rodolfo Berni, Michele Cianci

{"title":"Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus.","authors":"Michele Ferrari, Claudia Folli, Elisa Pincolini, Timothy S McClintock, Manfred Rössle, Rodolfo Berni, Michele Cianci","doi":"10.1107/S1744309112026103","DOIUrl":"10.1107/S1744309112026103","url":null,"abstract":"Crustacean crustacyanin proteins are linked to the production and modification of carapace colour, with direct implications for fitness and survival. Here, the structural and functional properties of the two recombinant crustacyanin subunits H(1) and H(2) from the American lobster Homarus americanus are reported. The two subunits are structurally highly similar to the corresponding natural apo crustacyanin CRTC and CRTA subunits from the European lobster H. gammarus. Reconstitution studies of the recombinant crustacyanin proteins H(1) and H(2) with astaxanthin reproduced the bathochromic shift of 85-95 nm typical of the natural crustacyanin subunits from H. gammarus in complex with astaxanthin. Moreover, correlations between the presence of crustacyanin genes in crustacean species and the resulting carapace colours with the spectral properties of the subunits in complex with astaxanthin confirmed this genotype-phenotype linkage.","PeriodicalId":7310,"journal":{"name":"Acta Crystallographica Section F-structural Biology and Crystallization Communications","volume":"68 Pt 8","pages":"846-53"},"PeriodicalIF":0.9,"publicationDate":"2012-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1107/S1744309112026103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30815443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline. 使用微聚焦光束线从含有多个微结构域的蛔虫suum As-p18脂肪酸结合蛋白晶体中获得可用的衍射数据。

IF 0.9 4区生物学

Acta Crystallographica Section F-structural Biology and Crystallization Communications Pub Date : 2012-08-01 Epub Date: 2012-07-31 DOI: 10.1107/S1744309112026553

Mads Gabrielsen, Alan Riboldi-Tunnicliffe, Marina Ibáñez-Shimabukuro, Kate Griffiths, Andrew J Roe, Alan Cooper, Brian O Smith, Betina Córsico, Malcolm W Kennedy

引用次数: 2

Binding of the unreactive substrate analog L-2-amino-3-guanidinopropionic acid (dinor-L-arginine) to human arginase I. 非活性底物类似物 L-2-氨基-3-胍基丙酸（dinor-L-精氨酸）与人类精氨酸酶 I 的结合。

IF 0.9 4区生物学

Acta Crystallographica Section F-structural Biology and Crystallization Communications Pub Date : 2012-08-01 Epub Date: 2012-07-27 DOI: 10.1107/S1744309112027820

Edward L D'Antonio, David W Christianson

引用次数: 0

A new crystal form of human histidine triad nucleotide-binding protein 1 (hHINT1) in complex with adenosine 5'-monophosphate at 1.38 Å resolution. 分辨率为 1.38 Å 的人组氨酸三核苷酸结合蛋白 1 (hHINT1) 与 5'- 磷酸腺苷复合物的新晶体形态。

IF 0.9 4区生物学

Acta Crystallographica Section F-structural Biology and Crystallization Communications Pub Date : 2012-08-01 Epub Date: 2012-07-27 DOI: 10.1107/S1744309112029491

Rafał Dolot, Magdalena Ozga, Artur Włodarczyk, Agnieszka Krakowiak, Barbara Nawrot

引用次数: 0

Expression, crystallization and preliminary crystallographic data analysis of PigF, an O-methyltransferase from the prodigiosin-synthetic pathway in Serratia. 沙雷氏菌中prodigiosin合成途径的o -甲基转移酶PigF的表达、结晶和初步晶体学数据分析。

IF 0.9 4区生物学

Acta Crystallographica Section F-structural Biology and Crystallization Communications Pub Date : 2012-08-01 Epub Date: 2012-07-31 DOI: 10.1107/S1744309112024001

Shaowei Liu, Tingting Ran, Xiang Shen, Langlai Xu, Weiwu Wang, Dongqing Xu

{"title":"Expression, crystallization and preliminary crystallographic data analysis of PigF, an O-methyltransferase from the prodigiosin-synthetic pathway in Serratia.","authors":"Shaowei Liu, Tingting Ran, Xiang Shen, Langlai Xu, Weiwu Wang, Dongqing Xu","doi":"10.1107/S1744309112024001","DOIUrl":"https://doi.org/10.1107/S1744309112024001","url":null,"abstract":"Prodigiosin, which is a member of the prodiginines, is a red linear tripyrrole compound. A gene cluster for the biosynthesis of prodigiosin has been identified in Serratia and most genes in the cluster have been functionally assigned. A bifurcated biosynthetic pathway for prodigiosin has previously been determined. The last step in the biosynthetic pathway of 4-methoxy-2,2'-bipyrrole-5-carbaldehyde (MBC) is catalyzed by PigF, which transfers a methyl group to 4-hydroxy-2,2'-bipyrrole-5-carbaldehyde (HBC) to form the terminal product MBC, but its catalytic mechanism is not known. To elucidate its mechanism, recombinant PigF was purified and crystallized. The crystals belonged to space group P2(1), with unit-cell parameters a = 69.4, b = 52.4, c = 279.2 Å, β = 96.8°. The native crystals may contain six molecules in the asymmetric unit, with a V(M) of 2.17 Å(3) Da(-1) and a solvent content of 43.43%. A full data set was collected at 2.6 Å resolution using synchrotron radiation on beamline BL17U of Shanghai Synchrotron Radiation Facility (SSRF), People's Republic of China. Molecular replacement was unsuccessful. To solve the structure of PigF by experimental phasing, selenomethionine-derivativized protein crystals were prepared from a condition with 0.01 M spermidine as an additive. One crystal diffracted to 1.9 Å resolution and a full data set was collected on beamline BL17U at SSRF. The crystal belonged to space group P2(1), with unit-cell parameters a = 69.0, b = 52.9, c = 93.4 Å, β = 97.3°. Heavy-atom substructure determination and phasing by SAD clearly showed that the crystal contains two molecules in the asymmetric unit, with a V(M) of 2.19 Å(3) Da(-1) and a solvent content of 43.82%.","PeriodicalId":7310,"journal":{"name":"Acta Crystallographica Section F-structural Biology and Crystallization Communications","volume":"68 Pt 8","pages":"898-901"},"PeriodicalIF":0.9,"publicationDate":"2012-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1107/S1744309112024001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30814869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Crystallization and preliminary X-ray studies of ferric uptake regulator from Magnetospirillum gryphiswaldense. Magnetospirillum gryphiswaldense 的铁吸收调节剂的结晶和初步 X 射线研究。

IF 0.9 4区生物学

Acta Crystallographica Section F-structural Biology and Crystallization Communications Pub Date : 2012-08-01 Epub Date: 2012-07-31 DOI: 10.1107/S1744309112024311

Zhao Liu, Zhongzhou Chen, Wei Wu

引用次数: 0

Crystallization and preliminary crystallographic studies of the YafN-YafO complex from Escherichia coli. 大肠杆菌YafN-YafO络合物的结晶及初步结晶学研究。

IF 0.9 4区生物学

Acta Crystallographica Section F-structural Biology and Crystallization Communications Pub Date : 2012-08-01 Epub Date: 2012-07-31 DOI: 10.1107/S1744309112024037

Fan Zhang, Li Xing, Maikun Teng, Xu Li

{"title":"Crystallization and preliminary crystallographic studies of the YafN-YafO complex from Escherichia coli.","authors":"Fan Zhang, Li Xing, Maikun Teng, Xu Li","doi":"10.1107/S1744309112024037","DOIUrl":"https://doi.org/10.1107/S1744309112024037","url":null,"abstract":"The ribosome-dependent mRNA interferase YafO from Escherichia coli belongs to a type II toxin-antitoxin (TA) system and its cognate antitoxin YafN neutralizes cell toxicity by forming a stable YafN-YafO complex. The YafN-YafO TA system is upregulated by the SOS response (a global response to DNA damage in which the cell cycle is arrested and mutagenesis is induced) and may then inhibit protein synthesis by endoribonuclease activity of YafO with the 50S ribosome subunit. Structural information on the YafN-YafO complex and related complexes would be helpful in order to understand the structural basis of the mechanism of mRNA recognition and cleavage, and the assembly of these complexes. Here, the YafN-YafO complex was expressed and crystallized. Crystals grown by the hanging-drop vapour-diffusion method diffracted to 3.50 Å resolution and belonged to the hexagonal space group P622, with unit-cell parameters a = 86.14, b = 86.14, c = 173.11 Å, α = β = 90, γ = 120°. Both Matthews coefficient analysis and the self-rotation function suggested the presence of one molecule per asymmetric unit in the crystal, with a solvent content of 65.69% (V(M) = 3.58 Å(3) Da(-1)).","PeriodicalId":7310,"journal":{"name":"Acta Crystallographica Section F-structural Biology and Crystallization Communications","volume":"68 Pt 8","pages":"894-7"},"PeriodicalIF":0.9,"publicationDate":"2012-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1107/S1744309112024037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30814868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Anomalous dispersion analysis of inhibitor flexibility: a case study of the kinase inhibitor H-89. 抑制剂灵活性的异常分散分析：激酶抑制剂 H-89 的案例研究。

IF 0.9 4区生物学

Acta Crystallographica Section F-structural Biology and Crystallization Communications Pub Date : 2012-08-01 Epub Date: 2012-07-26 DOI: 10.1107/S1744309112028655

Alexander Pflug, Kenneth A Johnson, Richard A Engh

{"title":"Anomalous dispersion analysis of inhibitor flexibility: a case study of the kinase inhibitor H-89.","authors":"Alexander Pflug, Kenneth A Johnson, Richard A Engh","doi":"10.1107/S1744309112028655","DOIUrl":"10.1107/S1744309112028655","url":null,"abstract":"With its ability to show the interactions between drug-target proteins and small-molecule ligands, X-ray crystallography is an essential tool in drug-discovery programmes. However, its usefulness can be limited by crystallization artifacts or by the data resolution, and in particular when assumptions of unimodal binding (and isotropic motion) do not apply. Discrepancies between the modelled crystal structure and the physiological range of structures generally prevent quantitative estimation of binding energies. Improved crystal structure resolution will often not aid energy estimation because the conditions which provide the highest rigidity and resolution are not likely to reflect physiological conditions. Instead, strategies must be employed to measure and model flexibility and multiple binding modes to supplement crystallographic information. One useful tool is the use of anomalous dispersion for small molecules that contain suitable atoms. Here, an analysis of the binding of the kinase inhibitor H-89 to protein kinase A (PKA) is presented. H-89 contains a bromobenzene moiety that apparently binds with multiple conformations in the kinase ATP pocket. Using anomalous dispersion methods, it was possible to resolve these conformations into two distinct binding geometries.","PeriodicalId":7310,"journal":{"name":"Acta Crystallographica Section F-structural Biology and Crystallization Communications","volume":"68 Pt 8","pages":"873-7"},"PeriodicalIF":0.9,"publicationDate":"2012-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30814864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystallization studies of the murine c-di-GMP sensor protein STING. 小鼠 c-di-GMP 传感蛋白 STING 的结晶研究。

IF 0.9 4区生物学

Acta Crystallographica Section F-structural Biology and Crystallization Communications Pub Date : 2012-08-01 Epub Date: 2012-07-31 DOI: 10.1107/S1744309112024372

Yi-Che Su, Zhi-Le Tu, Chao-Yu Yang, Ko-Hsin Chin, Mary Lay-Cheng Chuah, Zhao-Xun Liang, Shan-Ho Chou

{"title":"Crystallization studies of the murine c-di-GMP sensor protein STING.","authors":"Yi-Che Su, Zhi-Le Tu, Chao-Yu Yang, Ko-Hsin Chin, Mary Lay-Cheng Chuah, Zhao-Xun Liang, Shan-Ho Chou","doi":"10.1107/S1744309112024372","DOIUrl":"10.1107/S1744309112024372","url":null,"abstract":"The innate immune response is the first defence system against pathogenic microorganisms, and cytosolic detection of pathogen-derived DNA is believed to be one of the major mechanisms of interferon production. Recently, the mammalian ER membrane protein STING (stimulator of IFN genes; also known as MITA, ERIS, MPYS and TMEM173) has been found to be the master regulator linking the detection of cytosolic DNA to TANK-binding kinase 1 (TBK1) and its downstream transcription factor IFN regulatory factor 3 (IRF3). In addition, STING itself was soon discovered to be a direct sensor of bacterial cyclic dinucleotides such as c-di-GMP or c-di-AMP. However, structural studies of apo STING and its complexes with these cyclic dinucleotides and with other cognate binding proteins are essential in order to fully understand the roles played by STING in these crucial signalling pathways. In this manuscript, the successful crystallization of the C-terminal domain of murine STING (STING-CTD; residues 138-344) is reported. Native and SeMet-labelled crystals were obtained and diffracted to moderate resolutions of 2.39 and 2.2 Å, respectively.","PeriodicalId":7310,"journal":{"name":"Acta Crystallographica Section F-structural Biology and Crystallization Communications","volume":"68 Pt 8","pages":"906-10"},"PeriodicalIF":0.9,"publicationDate":"2012-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412770/pdf/f-68-00906.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30815941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0