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Acta biochimica et biophysica; Academiae Scientiarum Hungaricae最新文献

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Rotational dynamics of proteins in glycerinated muscle fibres. 甘油肌肉纤维中蛋白质的旋转动力学。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1984-01-01
J Belágyi, P Gróf
{"title":"Rotational dynamics of proteins in glycerinated muscle fibres.","authors":"J Belágyi, P Gróf","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17167889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescence polarization analysis of antigen determinant peptides of human fibrinogen. 人纤维蛋白原抗原决定肽的荧光极化分析。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1984-01-01
T Köszegi, M Kellermayer, K Jobst
{"title":"Fluorescence polarization analysis of antigen determinant peptides of human fibrinogen.","authors":"T Köszegi,&nbsp;M Kellermayer,&nbsp;K Jobst","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fluorescamine-labelled human fibrinogen was degraded with trypsin. Using fluorescence detection, the degradation products were fractionated by Sephadex G 50 gel filtration. Fragments of 3000-10 000 molecular weight were isolated for the fluorescence polarization studies. Specific antifibrinogen antibody increased the polarization of the isolated low molecular weight fragments. These results suggest that even the smallest proteolytic fragments of fibrinogen contain antigen determinant sequences. The fluorescence polarization method offers a sensitive and valuable approach for the analysis of the small antigenic peptides of human fibrinogen.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17152851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interaction of GGCC sequences of DNA with anticancer dianhydrogalacticol, detected by inhibition of restriction enzyme BspI. 抑制限制性内切酶BspI检测DNA GGCC序列与抗癌二氢半乳糖的相互作用。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1984-01-01
I Financsek, J Guczi, E J Hidvégi
{"title":"Interaction of GGCC sequences of DNA with anticancer dianhydrogalacticol, detected by inhibition of restriction enzyme BspI.","authors":"I Financsek,&nbsp;J Guczi,&nbsp;E J Hidvégi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Interaction of DNA with 1,2-5,6-dianhydro-galactitol (DAG, NSC 132 313), a bifunctional alkylating agent used in cancer therapy was studied. Treatment of lambda phage DNA with DAG in vitro protected some of the specific cleavage sites against the restriction enzyme BspI. The extent of protection depended on the concentration and time of DAG treatment but complete protection was not observed. Since the inactivation of the enzyme by DAG was excluded experimentally, the protection can be attributed to the binding of DAG to GGCC sequences of DNA. These experiments support the finding that guanine is the target of DNA alkylation by DAG (Institóris and Tamás, 1980). DAG treatment in vitro induced single strand breaks on DNA and this effect was also found to be concentration and time dependent.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17167887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of adenosine diphosphate on the interaction of actin-myosin-adenosine triphosphate. 二磷酸腺苷对肌动蛋白-肌球蛋白-三磷酸腺苷相互作用的影响。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1984-01-01
A Szöör, L Kónya, S Csabina
{"title":"The effect of adenosine diphosphate on the interaction of actin-myosin-adenosine triphosphate.","authors":"A Szöör,&nbsp;L Kónya,&nbsp;S Csabina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It was shown in experiments made on natural actomyosin of rabbit skeletal muscle that ADP possesses direct and indirect effects on superprecipitation. The presence of ADP increases the turbidity of the actomyosin suspension. An optimal ATP/ADP ratio may help the development and existence of a PC conformer of actomyosin corresponding to a myosin-ADP-actin-myosin-ATP complex. In this complex the extent of actin-activation is controlled by the cooperativity between the two myosin heads which, on the other side, may regulate in vivo the optimal transformation of the chemical energy of ATP for mechanical work.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17601987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Flow cytometric analysis of viability of bull sperm cells. 牛精细胞活力的流式细胞术分析。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1984-01-01
L Mátyus, G Szabó, I Resli, R Gáspár, S Damjanovich
{"title":"Flow cytometric analysis of viability of bull sperm cells.","authors":"L Mátyus,&nbsp;G Szabó,&nbsp;I Resli,&nbsp;R Gáspár,&nbsp;S Damjanovich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fluorescein diacetate and propidium iodide double fluorescence labeling of animal sperm cells in combination with 55 degrees C treatment for 15 min was used to monitor the viability and heat tolerance of sperm cells by flow cytometry. The applicability of the elaborated test to detect fertility differences has been shown on sperm samples obtained from bulls of known fertility values. According to preliminary examinations the method seems to be suitable to test the fertility of other male animals, too. The simplicity of the relatively quick procedure may provide a new screening method to qualify male animals before using their semen for large scale artificial insemination.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17602136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of external sodium on ouabain-insensitive K influx in fresh human red blood cells. 外用钠对新鲜人红细胞中乌阿巴因不敏感钾内流的影响。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1984-01-01
G Pfliegler, E Kelemen, B Szabó
{"title":"The effect of external sodium on ouabain-insensitive K influx in fresh human red blood cells.","authors":"G Pfliegler,&nbsp;E Kelemen,&nbsp;B Szabó","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The rate of 42K influx was investigated at various external sodium and ouabain concentrations in human red blood cells. In agreement with earlier reports, in red blood cells not treated with ouabain, Na did not affect K influx when [K]0 was 5.0 mM while it reduced the influx at [K]0 = 0.15 mM. However, Na stimulated 42K influx at both 5.0 mM and 0.15 mM in cells treated with ouabain (1 X 10(-5) M). When external Na concentration was raised from 0 to 72 mM the rate of 42K influx increased at [K]0 = 0.15 mM and at ouabain = 1 X 10(-5) M. The effect of external Na at different ouabain concentrations showed that K influx was inhibited by Na without or with ouabain in less than 5 X 10(-6) M while an increased K influx could be observed with higher ouabain concentrations in the incubation media. The results suggest that in the case of the complete inhibition of ouabain-sensitive K influx the electrochemical gradient of the Na ions may serve as a driving force for the inward movement of potassium.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17602143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Abstracts: 23rd meeting of the Hungarian Biochemical Society. 26-29 August, 1984, Pécs. 摘要:第23届匈牙利生化学会会议。1984年8月26-29日，psamacs。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1984-01-01
{"title":"Abstracts: 23rd meeting of the Hungarian Biochemical Society. 26-29 August, 1984, Pécs.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17566137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Long time stability of purple membranes from Halobacterium halobium. 嗜盐菌紫色膜的长时间稳定性。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1984-01-01
S G Hristova, G Váró, A Dér
{"title":"Long time stability of purple membranes from Halobacterium halobium.","authors":"S G Hristova,&nbsp;G Váró,&nbsp;A Dér","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The fast component of the electrical signal and the light absorption signals at different resolution times (at lambda = 408 nm) in an untreated purple membrane suspension were measured in order to study the stability of purple membrane fragments. It has been established that there is no change in the rate constants of L----M and M----O transitions of the photocycle in either the electrical or the light absorption signals of purple membrane suspension kept at 4 degrees C for up to 40 days. Moreover, also the amplitudes of the I (negative) and II (positive) components of the electrical signal remained unchanged during this period.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17602137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intermediate and zigzag (electronic) states (IS and ZZS) and quantum size effects (QSE) in finite crystals and biopolymers: proposals for experiments (short communication). 有限晶体和生物聚合物中的中间态和之形(电子)态(IS和ZZS)和量子尺寸效应(QSE):实验建议(短通信)。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1984-01-01
G Biczó
{"title":"Intermediate and zigzag (electronic) states (IS and ZZS) and quantum size effects (QSE) in finite crystals and biopolymers: proposals for experiments (short communication).","authors":"G Biczó","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17602142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
On the effect of the discrete nature of the photoelectric emission in the thermoluminescence measurements (pulse counting method). 热释光测量(脉冲计数法)中光电发射离散性的影响。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1984-01-01
P Zaránd, I Polgár
{"title":"On the effect of the discrete nature of the photoelectric emission in the thermoluminescence measurements (pulse counting method).","authors":"P Zaránd,&nbsp;I Polgár","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The discrete nature of the photoelectric emission cannot be neglected at low reading levels. The effect of A/D conversion after photomultiplication were both calculated and measured. The reading and its standard deviation were considerably different from those expected on the basis of a simple Poisson distribution and this should be taken into account at low reading levels. The authors describe a simple method which permits the experimental determination of photoelectron/digit conversion factor necessary for the prediction of the variation coefficient of TL measurements.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17602135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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