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Acta biochimica et biophysica; Academiae Scientiarum Hungaricae最新文献

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Investigation of the active center and catalytic mechanism of porcine kidney aminoacylase: a model of the active center. 猪肾氨基酰化酶活性中心及催化机理的研究:一个活性中心模型。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
B Szajáni, A Kiss, L Boross
{"title":"Investigation of the active center and catalytic mechanism of porcine kidney aminoacylase: a model of the active center.","authors":"B Szajáni,&nbsp;A Kiss,&nbsp;L Boross","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Procine kidney aminoacylase (E.C. 3.5.1.14) is inhibited neither by phenylmethylsulfonylfluoride nor by alkylating agents (iodoacetate or iodoacetamide). Therefore reaction mechanisms including the formation of acylenzyme through seryl or cysteinyl side chains are ruled out. The enzyme is a metalloprotein that can be inactivated by ECTA and in which Co2+ is an equivalent substitute for the Zn2+ ion. The two SH groups/subunit of aminoacylase exhibit different reactivites to p-hydroxymercuribenzoate. Modification of the less reactive SH group reversibly inactivates the enzyme. We suggest that this cysteinyl side chain is situated in the active center or in its immediate vicinity. On the basis of our results we suppose a close similarity between aminoacylase and carboxypeptidase A with respect to their active center and catalytic mechanism.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17831448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of antibiotics influencing membrane function on the potassium transport of E. coli cells. 抗生素对大肠杆菌细胞钾转运膜功能的影响。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
M Szögyi, I Tarján, G Tamás
{"title":"Effect of antibiotics influencing membrane function on the potassium transport of E. coli cells.","authors":"M Szögyi,&nbsp;I Tarján,&nbsp;G Tamás","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of polymixin, nigericin, gramicidin on the 42K-efflux of E. coli cells was studied. The 42K-efflux of the bacteria decreases in time according to an exponential function. The slopes of the linearized functions characterizing the efflux increase with increasing concentration of antibiotics. The frequency of events of the 42K-release as a parameter of antibiotics membrane interaction was determined on the basis of a theoretical model developed for the evaluation of our experimental data. In this way a quantitative comparison of the effectiveness of antibiotics was possible. We found, that the most effective antibiotic was polymixin, followed by nigericin and gramicidin.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17226986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes in the aerobic and anaerobic metabolism of skeletal muscle subjected to plaster cast immobilization. 石膏固定对骨骼肌有氧和无氧代谢的影响。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
I Edes, I Sohár, H Mazareán, O Takács, F Guba
{"title":"Changes in the aerobic and anaerobic metabolism of skeletal muscle subjected to plaster cast immobilization.","authors":"I Edes,&nbsp;I Sohár,&nbsp;H Mazareán,&nbsp;O Takács,&nbsp;F Guba","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of immobilization for different periods (7, 14, 28 and 42 days) on the aerobic and anaerobic metabolism of rabbit muscles with different functions was studied by measuring the total activity of LDH and MDH as well as the distribution of their isoenzymes. The results showed that on plaster casting the characteristic aerobic metabolism of m. soleus declined with a concomitant increase in glycolytic processes. In m. gastrocnemius the anaerobic metabolism of the muscle was relatively decreased on immobilization, but glycolysis remained the prevailing energy yielding process. On immobilization the metabolism of m. soleus and that of m. gastrocnemius approached each other. This may indicate a certain dedifferentiation of the muscle tissue.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18295953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantitative determination of thromboxane synthesis in blood samples by video-densitometry. 视频密度法定量测定血液样品中血栓素的合成。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
G Jécsai, S Bagdány
{"title":"Quantitative determination of thromboxane synthesis in blood samples by video-densitometry.","authors":"G Jécsai,&nbsp;S Bagdány","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18295950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Steroid spectrum in human urine as revealed by gas chromatography. III. Identification and quantitation of pregnenediol at different stages of bodily development. 气相色谱法测定人尿液中的类固醇光谱。3孕二醇在身体发育不同阶段的鉴定和定量。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
L Kecskés, Z Juricskay, G Czira
{"title":"Steroid spectrum in human urine as revealed by gas chromatography. III. Identification and quantitation of pregnenediol at different stages of bodily development.","authors":"L Kecskés,&nbsp;Z Juricskay,&nbsp;G Czira","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Gas chromatographic analysis of steroids using Sp-2100 stationary phase revealed that all urine samples from 34 healthy children (aged 4-14 years) and from 35 adults contained 5-pregnene-3 beta, 20 alpha-diol, which was also identified by GC-MS. The extent of pregnenediol excretion continuously increased from prepuberty to adult age. The observed increase in pregnenediol excretion during prepuberty points to an enhanced biosynthetic activity of the adrenal gland in the years preceding sexual maturation.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18466355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes in the intramembrane viscosity of sheep erythrocytes in the presence of membrane bound proteins. 膜结合蛋白存在时绵羊红细胞膜内黏度的变化。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
I Rédai, M Balázs, B Somogyi, S Damjanovich
{"title":"Changes in the intramembrane viscosity of sheep erythrocytes in the presence of membrane bound proteins.","authors":"I Rédai,&nbsp;M Balázs,&nbsp;B Somogyi,&nbsp;S Damjanovich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Some dynamic properties of protein- and antibody-treated cell membranes were investigated. The membrane viscosity of sheep erythrocytes increased upon binding various proteins as determined from the fluorescence emission anisotropy of 1,6-diphenyl 1,3,5-hexatriene dissolved in the membranes. However, specific, IgM type immunoglobulins produced against the erythrocytes decreased the intramembrane viscosity of cells. The applicability of the above dye to follow the binding of specific and non-specific proteins to erythrocytes is also discussed.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18456052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The action of non-ionic detergents on the extraction of phospholipids and proteins from rat liver and rabbit heart membranes. 非离子型洗涤剂对大鼠肝脏和兔心膜磷脂和蛋白质提取的影响。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
N X Thang, T Farkas, M Wollemann
{"title":"The action of non-ionic detergents on the extraction of phospholipids and proteins from rat liver and rabbit heart membranes.","authors":"N X Thang,&nbsp;T Farkas,&nbsp;M Wollemann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Triton X-100 and Lubrol PX at similar concentrations extracted more phospholipid than protein from rat liver and rabbit heart membranes, as compared with their original protein and phospholipid contents, respectively. All the types of membrane phospholipid were solubilized from rat liver membrane with the used non ionic detergents, but less sphingomyelin was extracted than phosphatidylcholine and phosphatidylethanolamine.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18456055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation of gamma-L-glutamyl-taurine from the protein free aqueous extract of bovine parathyroid powder. 牛甲状旁腺粉无蛋白水提物中γ - l-谷氨酰基牛磺酸的分离。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
A Furka, F Sebestyén, L Feuer, A Horváth, J Hercsel, S Ormai, B Bányai
{"title":"Isolation of gamma-L-glutamyl-taurine from the protein free aqueous extract of bovine parathyroid powder.","authors":"A Furka,&nbsp;F Sebestyén,&nbsp;L Feuer,&nbsp;A Horváth,&nbsp;J Hercsel,&nbsp;S Ormai,&nbsp;B Bányai","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18466354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A kinetic study of the K+ activated ATPase of myosin subfragment-1. 肌球蛋白亚片段-1 K+活化atp酶的动力学研究。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
G S Kelemen, K Pintér
{"title":"A kinetic study of the K+ activated ATPase of myosin subfragment-1.","authors":"G S Kelemen,&nbsp;K Pintér","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The K+ activated ATPase activity of myosin subfragment-1 was measured under different conditions and enzyme kinetic parameters were calculated. The logarithm of Km varies linearly with ionic strength down to very low KCl concentrations, the logarithm of k2 vs. ionic strength, on the other hand, considerably deviates from linearity at low concentrations of KCl. ADP is a competitive inhibitor, like myosin ATPase, and with practically the same inhibitor constant. The energetical parameters of the decomposition (to products and enzyme) of the S-1--ATP complex are partically the same as those for myosin, the parameters of its formation, however, differ from the corresponding values for myosin: delta SI is considerably, delta HI significantly higher in the case of myosin. This may be the result of some kind of interaction of the two heads of myosin.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17509750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Specificity of pancreatic elastase with tripeptidyl-p-nitroanilide substrates. 胰腺弹性酶对三肽基对硝基苯胺底物的特异性。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
G C Szabó, M Pozsgay, R Gáspár, P Elödi
{"title":"Specificity of pancreatic elastase with tripeptidyl-p-nitroanilide substrates.","authors":"G C Szabó,&nbsp;M Pozsgay,&nbsp;R Gáspár,&nbsp;P Elödi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>1. The kinetic properties of pancreatic elastase (E.C. 3.4.21.11) were investigated with 33 tripeptidyl-p-nitroanilide substrates, and the Km, kcat and kcat/Km values were determined. 2. The individual contributions of the substrate side chains to the kinetic constants were evaluated by regression analysis. As a result of the additivity of the contributions, the kinetic parameters of any substrate constructed from the amino acid investigated can be predicted. 3. Suc-D-Phe-Pro-Ala-pNA was the best substrate, as far as the Km (Michaelis constant) is concerned. The compound was synthesized and assayed. An excellent correlation was observed between its calculated (14 microM) and experimentally determined (15 microM) Km values. The aldehyde derivatives of this substrate is a competitive inhibitor of elastase (Ki = 0.6 mM). 4. The contribution values of the best substrates permitted us to characterize the topography of subsites involved in the formation of the enzyme-substrate complex. This, in turn, led us to the conclusion that the S3-P3 interaction is relatively less important in the binding of good substrates.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17994443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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