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Acta biochimica et biophysica; Academiae Scientiarum Hungaricae最新文献

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Physicochemical characterization of the cytosol glucocorticoid receptors in various lymphoid tissues. 不同淋巴组织中胞浆糖皮质激素受体的理化性质。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
P Arányi, A Náray
{"title":"Physicochemical characterization of the cytosol glucocorticoid receptors in various lymphoid tissues.","authors":"P Arányi,&nbsp;A Náray","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cytosol glucocorticoid receptors of five different lymphoid tissues of chicken and rats were characterized and compared by physicochemical parameters. Binding specificity of receptors was found to be similar, except that cytosol receptors of chick tissues appeared slightly more specific for dexamethasone than those from rats. Association rate constants of the dexamethasone-receptor complex were higher by about one order of magnitude in the case of chicken thymus and bursa than in other cytosols. Dissociation of dexamethasone-receptor complex occurred in one step in rat tissues, whereas in case of chicken tissues two phases of dissociation were discernible. Differences between Ka and the ratio of the association and dissociation rate constants are explained by non-equilibrium conditions. The stability of receptors from the two species differed significantly, the chick receptor being more stable. Hormone-receptor complex from each tissue could be separated into two fractions by DE-52 chromatography. The peak eluted at the lower KCl concentration in the case of chick corresponded to the fast dissociating hormone-receptor complex. Kinetic analyses proved to be just as useful as ion-exchange chromatography for the characterization of, and distinguishing between, glucocorticoid receptors.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18456053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescence energy transfer studies on normal and leukemic mouse lymphocytes. 正常和白血病小鼠淋巴细胞荧光能量转移研究。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
B Somogyi, P Kertai, S Damjanovich
{"title":"Fluorescence energy transfer studies on normal and leukemic mouse lymphocytes.","authors":"B Somogyi,&nbsp;P Kertai,&nbsp;S Damjanovich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Control and acute leukemic cells of AKR:Lac C3H mg:Lac/F1 hybride mice were labeled either with ethidium bromide or ethidium bromide plus fluorescamine. Theoretical and experimental evidence shows that the ethidium bromide binding ability of cells and the average efficiency of Förster-type fluorescence energy transfer between fluorescamine and ethidium bromide inside the cells depend on both the cell and dye concentrations. It is shown further that fluorescamine treatment enhances the uptake of ethidium bromide by both normal and leukemic cells. A sevenfold difference was found in ethidium bromide binding ability between normal and leukemic cells at optimal dye concentration.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17986299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new approach to studying cell-cell communication by the use of flow fluorimetry (short communication). 利用流动荧光法研究细胞间通讯的新方法(短通讯)。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
G Szabó, J Szöllösi, R Gáspár
{"title":"A new approach to studying cell-cell communication by the use of flow fluorimetry (short communication).","authors":"G Szabó,&nbsp;J Szöllösi,&nbsp;R Gáspár","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18456056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metal content of rat liver cell organelles. 大鼠肝细胞器的金属含量。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
A Ludány, M Kellermayer, K Jobst
{"title":"Metal content of rat liver cell organelles.","authors":"A Ludány,&nbsp;M Kellermayer,&nbsp;K Jobst","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Zn2+, Fe2+, Cu2+, Ca2+, and Mg2+ were determined by means of atomic absorption techniques in tetramethylammonium hydroxide digests of cell organelles isolated from normal rat liver tissue. The metal content of the cell organelles was expressed with reference to their protein content. The results showed that digestion with tetramethy la ammonium hydroxide can be applied for the determination of metal to protein ratios in the cell organelles and that these ratios appear to be suitable for the characterization of cell organelles.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18456059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
On the phase diagram of the dipalmitoyl phosphatidylcholine single-bilayer. Comparison with the multilamellar system. 双棕榈酰磷脂酰胆碱单双分子层的相图。与多层体系的比较。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
I P Sugár
{"title":"On the phase diagram of the dipalmitoyl phosphatidylcholine single-bilayer. Comparison with the multilamellar system.","authors":"I P Sugár","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17942433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Video-fluorimetric evaluation of human blood serum electrophoretograms (short communication). 人血清电泳图的视频荧光评价(短通信)。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
A Dán, R Gáspár, J Matkó, S Damjanovich
{"title":"Video-fluorimetric evaluation of human blood serum electrophoretograms (short communication).","authors":"A Dán,&nbsp;R Gáspár,&nbsp;J Matkó,&nbsp;S Damjanovich","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18456054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantitative evaluation of ion-exchange thin-layer chromatograms by video-densitometry (IV.) Determination of Diaminopimelic acid. 离子交换薄层色谱的定量评价(四)二氨基苯甲酸的测定。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
S Pongor, K Baintner
{"title":"Quantitative evaluation of ion-exchange thin-layer chromatograms by video-densitometry (IV.) Determination of Diaminopimelic acid.","authors":"S Pongor,&nbsp;K Baintner","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18466353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chromatographic study of the UV photoproducts in uracil solid phase systems. 紫外光产物在尿嘧啶固相体系中的色谱研究。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
J Fidy, S Gáspár, K Raksányi
{"title":"Chromatographic study of the UV photoproducts in uracil solid phase systems.","authors":"J Fidy,&nbsp;S Gáspár,&nbsp;K Raksányi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>UV photoproducts were induced in two types of uracil solid phase systems: in evaporated and recrystallized thin layers and in dried aquaeous solution respectively. The nature of the photochemical reactions in the two systems was compared by gradient elution column chromatography. In the case of dried solution two types of cyclobutane dimers were isolated, but in recrystallized thin layers only one type was found. The efficiency of the reaction in evaporated layers was much higher than in those produced by drying. Based on the crystal structure of uracil and previous absorption spectroscopic studies of the photochemistry of thin crystalline layers it was stated, that the single type of photodimers produced in evaporated thin layers is characteristic for the photochemical reaction proceeding in crystal matrix; and its structure was proposed to be identical with that of the cis-syn stereoisomer dimer.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18466356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Studies on the heat inactivation of porcine kidney aminoacylase. 猪肾氨基酰化酶热失活的研究。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
B Szajáni
{"title":"Studies on the heat inactivation of porcine kidney aminoacylase.","authors":"B Szajáni","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Heat inactivation of porcine kidney aminoacylase (E.C.3.5.1.14) was investigated under different conditions i.e. at different temperatures, protein concentrations and pH values). Kinetic analysis of the inactivation process suggests that aminoacylase is a dissociable enzyme: the dimeric form is dissociated to enzymatically active monomers and the heat stability of the dimer is higher than that of the monomers. High temperatures, dilution and pH values other than neutral, all promote dissociation. The KDapp at 60 degrees C, pH 7.0 is of the order of 10(-6) M.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18456058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
K and Ca in muscle mechanics, II. K-contracture. 肌肉力学中的K和Ca, 2。K-contracture。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1980-01-01
R Varga-Mányi
{"title":"K and Ca in muscle mechanics, II. K-contracture.","authors":"R Varga-Mányi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>K-rich solution, the isotony of which corresponds to normal Ringer's solution, was used in our experiments: 4.59 g KCl + 40.5 g saccharose, 1000 ml bidistilled water. Freshly prepared living muscles of Rana esculenta (m. sartorius, m. iliofibularis, m. peroneus, m. tibialis, m. gastrocnemius, m. semimembranosus) got into K-contracture in this solution, but they relaxed within some minutes. In muscles of dead frog (which canno tbe stimulated with 110 V a.c.) the K-rich solution did not elicit contracture, i.e. on the one hand K-contracture could be observed only in living muscle and on the other hand, the muscles did not lose their excitability after the K-contracture had ceased (a.c. 2V).</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18019100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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