抑制限制性内切酶BspI检测DNA GGCC序列与抗癌二氢半乳糖的相互作用。

I Financsek, J Guczi, E J Hidvégi
{"title":"抑制限制性内切酶BspI检测DNA GGCC序列与抗癌二氢半乳糖的相互作用。","authors":"I Financsek,&nbsp;J Guczi,&nbsp;E J Hidvégi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Interaction of DNA with 1,2-5,6-dianhydro-galactitol (DAG, NSC 132 313), a bifunctional alkylating agent used in cancer therapy was studied. Treatment of lambda phage DNA with DAG in vitro protected some of the specific cleavage sites against the restriction enzyme BspI. The extent of protection depended on the concentration and time of DAG treatment but complete protection was not observed. Since the inactivation of the enzyme by DAG was excluded experimentally, the protection can be attributed to the binding of DAG to GGCC sequences of DNA. These experiments support the finding that guanine is the target of DNA alkylation by DAG (Institóris and Tamás, 1980). DAG treatment in vitro induced single strand breaks on DNA and this effect was also found to be concentration and time dependent.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Interaction of GGCC sequences of DNA with anticancer dianhydrogalacticol, detected by inhibition of restriction enzyme BspI.\",\"authors\":\"I Financsek,&nbsp;J Guczi,&nbsp;E J Hidvégi\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Interaction of DNA with 1,2-5,6-dianhydro-galactitol (DAG, NSC 132 313), a bifunctional alkylating agent used in cancer therapy was studied. Treatment of lambda phage DNA with DAG in vitro protected some of the specific cleavage sites against the restriction enzyme BspI. The extent of protection depended on the concentration and time of DAG treatment but complete protection was not observed. Since the inactivation of the enzyme by DAG was excluded experimentally, the protection can be attributed to the binding of DAG to GGCC sequences of DNA. These experiments support the finding that guanine is the target of DNA alkylation by DAG (Institóris and Tamás, 1980). DAG treatment in vitro induced single strand breaks on DNA and this effect was also found to be concentration and time dependent.</p>\",\"PeriodicalId\":7308,\"journal\":{\"name\":\"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

研究了DNA与用于肿瘤治疗的双功能烷基化剂1,2,5,6 -二氢半乳糖醇(DAG, NSC 132 313)的相互作用。在体外用DAG处理lambda噬菌体DNA可以保护一些特定的裂解位点免受限制性内切酶BspI的影响。保护程度取决于DAG处理的浓度和时间,但未观察到完全保护。由于实验排除了DAG对酶的失活作用,因此这种保护作用可归因于DAG与DNA GGCC序列的结合。这些实验支持了鸟嘌呤是DAG DNA烷基化的目标的发现(Institóris and Tamás, 1980)。体外DAG处理诱导DNA单链断裂,这种效应也被发现是浓度和时间依赖的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Interaction of GGCC sequences of DNA with anticancer dianhydrogalacticol, detected by inhibition of restriction enzyme BspI.

Interaction of DNA with 1,2-5,6-dianhydro-galactitol (DAG, NSC 132 313), a bifunctional alkylating agent used in cancer therapy was studied. Treatment of lambda phage DNA with DAG in vitro protected some of the specific cleavage sites against the restriction enzyme BspI. The extent of protection depended on the concentration and time of DAG treatment but complete protection was not observed. Since the inactivation of the enzyme by DAG was excluded experimentally, the protection can be attributed to the binding of DAG to GGCC sequences of DNA. These experiments support the finding that guanine is the target of DNA alkylation by DAG (Institóris and Tamás, 1980). DAG treatment in vitro induced single strand breaks on DNA and this effect was also found to be concentration and time dependent.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信