Frontiers in fungal biology最新文献

{"title":"Integrating beneficial microorganisms and soil amendment for grapevine health: toward eco-friendly seasonal fungal disease management and soil improvement.","authors":"Lobna Hajji-Hedfi, Takwa Wannassi, Samar Dali, Wissem Hamdi, Boulbaba L'taief, Ahmed M Abdel-Azeem","doi":"10.3389/ffunb.2025.1713132","DOIUrl":"10.3389/ffunb.2025.1713132","url":null,"abstract":"<p><p>This study evaluates the effectiveness of two biological agents, <i>Trichoderma longibrachiatum</i> and <i>Pseudomonas yamanorum</i>, together with compost addition. The results show that combining compost with the microbial consortium enhances the physicochemical properties of the soil by increasing nitrogen, carbon, and organic matter while reducing bulk density and nitrate levels. Furthermore, this combination stimulates plant defense mechanisms, leading to increased antioxidant enzyme activity and higher phenolic compound levels. The amendments also improved critical soil properties, increasing organic matter (up to 4.14%), organic carbon (up to 2.40%), and total nitrogen (up to 1.47 mg/g), suggesting that these effects may be linked to the richness of microorganisms in the compost. The combined treatments also reduced the symptoms of fungal diseases: the severity of <i>Botrytis cinerea</i> decreased from 82%-92% to 4.97%-7.71%; <i>Erysiphe necator</i> from 89%-95% to 2.34%-8.03%; and <i>Plasmopara viticola</i> from 70%-95% to 2.84%-5.66%. In conclusion, the use of compost and beneficial microorganisms as bio-stimulants could offer an effective and sustainable solution for improving grapevine soil quality and managing fungal diseases.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"6 ","pages":"1713132"},"PeriodicalIF":3.8,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12728585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145835554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and genomic characterization of azole resistance in Portuguese <i>Candida parapsilosis</i> isolates.","authors":"Alexandru-Marian Papuc, Cristina Veríssimo, Helena Simões, Cristina Toscano, João Paulo Gomes, Verónica Mixão, Raquel Sabino","doi":"10.3389/ffunb.2025.1713961","DOIUrl":"10.3389/ffunb.2025.1713961","url":null,"abstract":"<p><strong>Introduction: </strong><i>Candida parapsilosis</i> is a clinically important etiological agent of systemic infections associated with hospital outbreaks, which prevalence has been increasing in the last decade. Moreover, in recent years, fluconazole resistance in this species has been emerging in different countries, being a subject of significant interest and concern. In this context, the present study aims to determine the frequency of fluconazole resistance in <i>C. parapsilosis</i> sensu stricto isolates collected in Portugal (2003-2007 and 2017-2024), understand its associated molecular mechanisms, and relate it all with worldwide genomic data.</p><p><strong>Methods: </strong>To this end, we performed phenotypic assays of 145 isolates of <i>C. parapsilosis</i> collected from different biological and environmental products in Portugal (majority from the Lisbon Metropolitan area), and explored the genomic features of the fluconazole-resistant ones.</p><p><strong>Results: </strong>We found eight <i>C. parapsilosis</i> fluconazole-resistant isolates between 2017 and 2024, corresponding to a frequency of 8.5% in this period, and contrasting with the absence of fluconazole-resistant isolates collected before 2007. Sequencing of the <i>ERG11</i> gene showed that all fluconazole-resistant isolates had the Y132F and R398I mutations.</p><p><strong>Discussion: </strong>A phylogenomic analysis including publicly available isolates from other countries revealed that our Portuguese isolates are more closely related to those from the USA and Germany than to the isolates sequenced thus far from the neighbor country, Spain. Furthermore, although three distinct <i>C. parapsilosis</i> genetic clades were found in our dataset, all the fluconazole-resistant isolates detected in this study cluster together, raising the question of whether the increased fluconazole-resistance in the country could possibly be associated with the emergence or introduction of this particular lineage. Altogether, these results provide valuable insights on fluconazole resistance in a set of Portuguese <i>C. parapsilosis</i> isolates and their associated mechanisms, representing an important step towards a better understanding of the increasing <i>C. parapsilosis</i> fluconazole resistance in Southern Europe.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"6 ","pages":"1713961"},"PeriodicalIF":3.8,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145835517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-resolved tracking of <i>Penicillium commune</i> in a bakery facility highlights long-term environmental persistence.","authors":"Annette Fagerlund, Charlotte Kummen, Anette Wold Åsli, Cathrine Kure Finne","doi":"10.3389/ffunb.2025.1712444","DOIUrl":"10.3389/ffunb.2025.1712444","url":null,"abstract":"<p><p>Mould spoilage is a major challenge in bakery production, yet the sources and persistence of contaminating strains remain poorly understood. We applied whole-genome sequencing (WGS) to 68 isolates from potato-cereal wraps and their production environment in a Norwegian bakery. Barcode-based identification using ITS, <i>BenA</i>, <i>CaM</i>, and <i>RPB2</i> confirmed that 65 isolates belonged to the <i>Penicillium commune</i>/<i>Penicillium fuscoglaucum</i> lineage but could not fully resolve species status or resolve strain-level differences. Genome-wide comparison using Mash placed these isolates in a single clade within series <i>Camembertiorum</i>, distinct from cheese-associated taxa. SNP analysis revealed extremely low diversity within the main cluster (up to 60 SNPs after recombination filtering) and demonstrated that genetically similar strains persisted in the facility for 15 months, spanning multiple products and environmental samples. No consistent association with potato suppliers or production dates was detected, indicating that long-term environmental reservoirs were the main source of contamination. These findings show that persistent clonal lineages can survive routine cleaning in dry bakery environments, enabling recurrent contamination. WGS provided the strain-level resolution needed to uncover this persistence and clarify phylogenetic placement, underscoring its value for monitoring and controlling mould spoilage in food production.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"6 ","pages":"1712444"},"PeriodicalIF":3.8,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12714999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145806306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beauvericin production by endophytic and epiphytic <i>Beauveria bassiana</i> in peach (<i>Prunus persica</i>) and implications for insect biocontrol.","authors":"Sabrina A Elgar, Caterina Villari, Conor G Fair, David I Shapiro-Ilan, Dario Chavez, Brett R Blaauw","doi":"10.3389/ffunb.2025.1714008","DOIUrl":"10.3389/ffunb.2025.1714008","url":null,"abstract":"<p><p><i>Beauveria bassiana</i> (Balsamo) Vuillemin is a well-known entomopathogenic fungus that occupies diverse ecological niches, including soilborne, epiphytic, and endophytic habitats. Its capacity to function as an endophyte has received growing interest in potential applications for sustainable pest management, particularly in woody perennial systems where delivery and persistence of biological control agents are challenging. This study investigated endophytic colonization of peach (<i>Prunus persica</i> Batsch) seedlings by <i>B. bassiana</i> and quantified production of the insecticidal secondary metabolite beauvericin (BEA) in and on plant tissues. Seedlings were inoculated via foliar spray or soil drench. Fungal recovery was assessed from leaf, stem, and root tissues. Colonization patterns indicated systemic movement, however foliar spray increased recovery from leaf tissues and soil drench increased recovery from roots over time. BEA concentrations varied significantly by tissue type, inoculation method, and surface sterilization status. The highest levels were detected in non-surface-sterilized leaves of foliar-sprayed plants, measured two weeks post-inoculation. Surface sterilization prior to extraction significantly reduced detected concentrations, suggesting that BEA is primarily produced by epiphytic fungal growth. Larval bioassays with <i>Tenebrio molitor</i> L. revealed increased mortality associated with foliar-sprayed tissues, aligning with observed BEA levels and suggesting localized insecticidal activity. These findings demonstrate that the spatial dynamics of fungal colonization and metabolite localization are critical considerations for the effective deployment of <i>B. bassiana</i> in biocontrol strategies. Further research is needed to determine how environmental factors, host physiology, fungal strain, and time influence secondary metabolite production in and on plants treated with <i>B. bassiana</i>.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"6 ","pages":"1714008"},"PeriodicalIF":3.8,"publicationDate":"2025-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12695560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145758513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LED light spectra influence the stimulation of mycelial growth and anticancer activity in <i>Hericium erinaceus</i> mycelium.","authors":"Preuk Chutimanukul, Siripong Sukdee, Pawarisa Phetkaew, Ornprapa Thepsilvisut, Onmanee Prajuabjinda","doi":"10.3389/ffunb.2025.1684852","DOIUrl":"10.3389/ffunb.2025.1684852","url":null,"abstract":"<p><strong>Introduction: </strong><i>Hericium erinaceus</i> is a medicinal mushroom known for producing diverse bioactive metabolites with therapeutic potential. However, cultivation strategies aimed at enhancing both fungal yield and metabolite bioactivity, particularly through light-mediated physiological modulation, remain insufficiently investigated. This study explored the influence of different LED light spectra on the growth performance and cytotoxic potential of <i>H. erinaceus</i> mycelia cultivated on a nutrient-rich red sorghum substrate.</p><p><strong>Methods: </strong>Mycelia were cultivated for 30 days under four LED light spectra-blue, red, green, and RGB, compared to a control treatment (which was kept in darkness). Growth parameters, including radial growth rate, colonization speed, fresh weight, biomass increase, and mycelial density, were recorded at harvest. Ethanol extracts prepared from the mycelia of each treatment were tested for cytotoxic activity against SW480 colorectal cancer cells, HepG2 liver cancer cells, and normal colon epithelial cells (CCD-841 CoN), and IC₅₀ values were determined.</p><p><strong>Results and discussion: </strong>Blue light produced the most pronounced enhancement in growth performance, yielding the highest mycelial density (0.344 g/cm²), fresh weight (6.75 g), and biomass increase (12.28%), along with the fastest radial expansion and substrate colonization. Extracts from blue light-treated mycelia showed the strongest cytotoxic effects against SW480 (IC₅₀ = 133.71 μg/mL) and HepG2 cells (IC₅₀ = 114.84 μg/mL), while exerting minimal effects on normal CCD-841 CoN cells. These findings suggest that targeted light spectra can modulate fungal physiology, likely via photoreceptor-mediated pathways, to enhance both agronomic performance and production of cytotoxic metabolites. This study provides a framework for optimizing <i>H. erinaceus</i> cultivation for functional food and therapeutic applications.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"6 ","pages":"1684852"},"PeriodicalIF":3.8,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12689372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145745918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrophobins in <i>Bipolaris maydis</i> do not contribute to colony hydrophobicity, but their heterologous expressions alter colony hydrophobicity in <i>Aspergillus nidulans</i>.","authors":"Kenya Tsuji, Hiroshi Yoshida, Masafumi Saba, Yuki Terauchi, Moriyuki Kawauchi, Yoichi Honda, Chihiro Tanaka, Akira Yoshimi","doi":"10.3389/ffunb.2025.1604903","DOIUrl":"10.3389/ffunb.2025.1604903","url":null,"abstract":"<p><p>Hydrophobins are small amphiphilic proteins secreted by filamentous fungi. These proteins confer hydrophobic properties to the hyphae and conidia. <i>Bipolaris maydis</i> is the causal agent of southern corn leaf blight; the biological function of its hydrophobins is not clear. In the present study, we focused on the broad function of hydrophobins in the life cycle of this fungus. We found that the <i>B. maydis</i> genome encodes four hydrophobins-Hyp1 of class I, and Hyp2, Hyp3 and Hyp4 of class II-and all of them are expressed. We generated single disruptants of each gene, as well as triple and quadruple disruptants. No differences were detected between the wild type and any of disruptants in mycelial growth, conidiation, stress tolerance, virulence, or sexual reproduction. The colony hydrophobicity of all disruptant strains was similar to that of the wild-type strain. Complementation of a null <i>Aspergillus nidulans</i> mutant of <i>dewA</i>, which showed a significantly reduced colony hydrophobicity, with each of the four <i>B. maydis</i> hydrophobin genes restored the hydrophobic phenotype, although the degree of hydrophobicity varied among them. Despite the absence of any significant phenotypic changes in the <i>B. maydis</i> mutants generated, results strongly suggest that all four hydrophobins have retained their function in hydrophobicity. Furthermore, the results of this study suggest that the role of hydrophobins might change depending on the fungal species.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"6 ","pages":"1604903"},"PeriodicalIF":3.8,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12675215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145703058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential gene expression in ripe mango fruit (<i>Mangifera indica</i> L. cv. Azúcar) that favors the pathogenicity of the endophyte <i>Colletotrichum tropicale</i>.","authors":"Andrés F Quintero-Mercado, Sebastián Rojas, Yordan J Romero-Contreras, Luis F Lozano, Mario Serrano, Celsa García","doi":"10.3389/ffunb.2025.1699983","DOIUrl":"10.3389/ffunb.2025.1699983","url":null,"abstract":"<p><p><i>Colletotrichum tropicale</i> is an endophyte that has been reported as a pathogen in ripe mango fruits (Mangifera indica L. cv. Azúcar) in Magdalena, Colombia, causing anthracnose. However, gene expression in the host that promotes its lifestyle transition remains unknown. This study aimed to analyze gene expression during the interaction between ripe mango fruit cv. Azúcar and <i>C. tropicale</i> to identify differentially expressed host genes that facilitate the pathogen's infection process. RNA sequencing (RNA-seq) analysis was conducted at 0 and 12 h post inoculation (hpi), including <i>de novo</i> assembly and bioinformatic functional annotation using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). A total of 5,435 differentially expressed genes (DEGs) were identified in the interaction, of which only 421 DEGs were detected in ripe mango fruits. Among these, 379 were upregulated and 42 were downregulated (T0 vs. T12 hpi). GO functional annotation of downregulated fruit genes revealed that the molecular functions affected at 12 hpi were related to the plant's defensive oxidative burst mediated by reactive oxygen species (ROS)-including NADPH oxidase activity, hydrogen peroxide formation, and the action of peroxidases and oxidoreductase enzymes-whereas upregulated genes were associated with stress response, defense, transferase activity, and kinase activity. KEGG analysis identified pathways related to mitogen-activated protein kinase (MAPK) signaling, pathogen-associated molecular pattern (PAMP)-triggered immunity, and phenylalanine metabolism. In conclusion, ripe mango fruit cv. Azúcar activates a defense response against <i>C. tropicale</i> at 12 hpi that does not overcome the pathogen's initial quiescent phase but instead facilitates conditions for its establishment by suppressing oxidative burst pathways, which may later contribute to oxidative stress during the necrotrophic phase.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"6 ","pages":"1699983"},"PeriodicalIF":3.8,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12631287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145589956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel and advanced MNP molecular markers accurately identify the genetic similarity of <i>Hypsizygus marmoreus</i> strains: a comparative evaluation with ISSR and antagonistic methods.","authors":"Honglei Zhao, Chuanzheng Wei, Tuo Zhang, Yihao Zhao, Xinyue Chen, Zhiwen Lv, Menghan Nie, Jian Li, Yuanzhen Liu, Baogui Xie, Xinrui Liu","doi":"10.3389/ffunb.2025.1647126","DOIUrl":"10.3389/ffunb.2025.1647126","url":null,"abstract":"<p><p><i>Hypsizygus marmoreus</i> is a wood-rotting fungus of significant medicinal value, extensively cultivated industrially. As the scale of production expands, the issue of \"same strains with different names\" has become increasingly important, necessitating an accurate, efficient, and rapid method for variety identification. In this study, we resequenced 79 strains of <i>H. marmoreus</i> and selected 32 strains to construct a database comprised of 369 multiple nucleotide polymorphism (MNP) molecular markers and subsequently analyzed the genetic similarity among these strains. The results revealed that none of the 32 selected strains exhibited 100% genetic similarity. Specifically, the genetic similarity of the 369 MNP markers among the white strains ranged from 11.92% to 88.62%, while that of the gray strains ranged from 2.71% to 74.53%, indicating that the gray strains exhibited greater genetic diversity than their white counterparts. Furthermore, we compared the identification results of MNP molecular markers with those obtained from cross-plating experiments and ISSR molecular markers. This comparison highlighted the advantages of the MNP molecular marker method in terms of stability, accuracy, and high efficiency, thereby significantly contributing to the advancement of <i>H. marmoreus</i> strains identification and creation.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"6 ","pages":"1647126"},"PeriodicalIF":3.8,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12593490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145483950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological advancements enabling cannabinoid biosynthesis in engineered fungi: a mini review.","authors":"Madira Coutlyne Manganyi, Christ Donald Kaptchouang Tchatchouang","doi":"10.3389/ffunb.2025.1660661","DOIUrl":"10.3389/ffunb.2025.1660661","url":null,"abstract":"<p><p>Cannabinoids, such as Δ⁹tetrahydrocannabinol (THC) and cannabidiol (CBD), are bioactive compounds with well-documented therapeutic potential, including applications in pain relief, neuroprotection, anti-inflammatory treatments, and seizure control. Traditionally sourced from <i>Cannabis</i> plants, their production remains limited by agricultural constraints, regulatory hurdles, and environmental concerns. In response, recent advances in biotechnology have enabled the microbial biosynthesis of cannabinoids, offering a scalable and sustainable alternative. Engineered fungi, in particular, have gained attention as promising production platforms due to their metabolic flexibility, ease of genetic manipulation, and capacity for synthesizing complex secondary metabolites. This mini-review explores key innovations in synthetic biology and metabolic engineering that have enabled fungal cannabinoid biosynthesis. It highlights strategies such as pathway reconstruction, enzyme optimization, host strain engineering, and the application of CRISPR-Cas9 genome editing. In addition, it examines ongoing challenges, including product toxicity, metabolic burden, and regulatory considerations. Finally, the review outlines future directions in systems biology, the production of rare cannabinoids, and bioprocess optimization. Overall, the development of engineered fungi for cannabinoid biosynthesis represents a major conceptual advance in microbial biotechnology, with far-reaching implications for the pharmaceutical, nutraceutical, and industrial sectors.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"6 ","pages":"1660661"},"PeriodicalIF":4.3,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12592125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145483998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in nomenclature, virulence factors, and antifungal resistance of the genus <i>Candida</i>.","authors":"Maria Alyce Albuquerque Fernandes, Francisca Lidiane Linhares de Aguiar, Maria Gleiciane Soares Coutinho, Erika Helena Salles de Brito, Camila Gomes Virginio Coelho, Raquel Oliveira Dos Santos Fontenelle","doi":"10.3389/ffunb.2025.1677892","DOIUrl":"10.3389/ffunb.2025.1677892","url":null,"abstract":"<p><p>Some <i>Candida</i> species of clinical interest have undergone recent nomenclature changes. These yeasts have a high capacity to adhere to and infect host tissues, driven by their virulence factors, as well as by the incidence of antifungal resistance. This review aimed to analyze the taxonomic changes of the main species of clinical interest within the <i>Candida</i> genus, considering the clinical implications of their virulence factors and the main mechanisms of antifungal resistance. The research results allowed us to understand that the updated nomenclature of <i>Candida</i> species is essential to maintain the criteria that define a genus, organizing the species according to their phylogenetic and evolutionary characteristics. Understanding the virulence factors and resistance mechanisms of the different species of clinical interest helps us understand how infections are initiated and established, as well as how these same species behave to neutralize the action of antifungals. Therefore, integrating knowledge of taxonomy, virulence, and resistance profiles is crucial for effective strategies to control and treat fungal infections.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"6 ","pages":"1677892"},"PeriodicalIF":3.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12569811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145410977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}