F&S science最新文献

{"title":"Engineered uterine primary myometrial cells with high-mobility group AT-hook 2 overexpression display a leiomyoma-like transcriptional and epigenomic phenotype","authors":"Priyanka Saini Ph.D. ,&nbsp;Austin G. Holmes Ph.D. ,&nbsp;Jian-Jun Wei M.D. ,&nbsp;J. Brandon Parker Ph.D. ,&nbsp;Debabrata Chakravarti Ph.D.","doi":"10.1016/j.xfss.2024.07.008","DOIUrl":"10.1016/j.xfss.2024.07.008","url":null,"abstract":"<div><h3>Objective</h3><div>To determine if engineered high-mobility group AT-hook 2 (HMGA2) overexpressing uterine primary myometrial cells recapitulate the transcriptional and epigenomic features of HMGA2-subtype leiomyomas.</div></div><div><h3>Design</h3><div>Isolated primary, “normal” myometrial cells from three patients were engineered to overexpress HMGA2 to determine how HMGA2 establishes transcriptomic and epigenomic features of HMGA2-overexpressing leiomyoma.</div></div><div><h3>Setting</h3><div>Academic research laboratory.</div></div><div><h3>Patient(s)</h3><div>Primary myometrial cells were isolated from normal myometrium obtained from three patients undergoing hysterectomy.</div></div><div><h3>Intervention(s)</h3><div>Not applicable.</div></div><div><h3>Main Outcome Measure(s)</h3><div>Determined genome-wide transcriptomic and epigenomic features of engineered HMGA2-overexpressing uterine primary myometrial cells.</div></div><div><h3>Result(s)</h3><div>Engineered HMGA2-V5-overexpressing primary myometrial cells approximated the HMGA2 expression level observed in HMGA2-overexpression subtype leiomyoma. High-mobility group AT-hook 2-V5 expression resulted in differential expression of 1,612 genes (false discovery rate [FDR] &lt; 0.05) that were found to be enriched in pathways associated with leiomyoma formation, including extracellular matrix organization. Comparative gene expression analysis between HMGA2-V5 engineered primary cells and HMGA2-overexpression subtype leiomyoma revealed significant overlap of differentially expressed genes. Mechanistically, HMGA2-V5 overexpression resulted in 41,323 regions with differential H3K27ac deposition (FDR &lt; 0.05) and 205,605 regions of altered chromatin accessibility (FDR &lt; 0.05). Transcription factor binding site analysis implicated the AP-1 family of transcription factors.</div></div><div><h3>Conclusion(s)</h3><div>High-mobility group AT-hook 2 overexpression induces leiomyoma-like transcriptomic and epigenomic modulations in myometrial cells.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"5 4","pages":"Pages 352-368"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141794185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiling of the oocyte-cumulus-granulosa cell complex from estrogen receptor β knockout mice","authors":"Virpi Töhönen Ph.D. ,&nbsp;Per Antonson Ph.D. ,&nbsp;Nageswara Rao Boggavarapu Ph.D. ,&nbsp;Heba Ali M.Sc. ,&nbsp;Leticia Apolinario Motaholi Ph.D. ,&nbsp;Jan-Åke Gustafsson Ph.D. ,&nbsp;Mukesh Varshney Ph.D. ,&nbsp;Kenny A. Rodriguez-Wallberg Ph.D. ,&nbsp;Shintaro Katayama Ph.D. ,&nbsp;Ivan Nalvarte Ph.D. ,&nbsp;Jose Inzunza Ph.D.","doi":"10.1016/j.xfss.2024.08.004","DOIUrl":"10.1016/j.xfss.2024.08.004","url":null,"abstract":"<div><h3>Objective</h3><div>To study the role of estrogen receptor β in follicle development and maturation and the response to gonadotropin stimulation aiming at superovulation.</div></div><div><h3>Design</h3><div>Experimental study and transcriptomic analysis.</div></div><div><h3>Setting</h3><div>Karolinka Institutet, medical university.</div></div><div><h3>Animal(s)</h3><div>Healthy wild-type (WT) and estrogen receptor β knockout (<em>Esr</em>2-KO) female mice undergoing superovulation at 4 weeks, 7 weeks, and 6 months of age.</div></div><div><h3>Intervention(s)</h3><div>Not applicable.</div></div><div><h3>Main Outcome Measure(s)</h3><div>Oocyte yield after superovulation, transcriptomic profiling of cumulus-granulosa cell complexes and oocytes, and immunohistochemical analyses.</div></div><div><h3>Result(s)</h3><div>Superovulation of estrogen receptor β (ERβ) knockout mice resulted in reduced oocyte yield at 6 months of age compared with WT mice, but younger mice had similar yields. RNA-seq analysis of cumulus cells from superovulated WT and <em>Esr</em>2-KO mice identified genes and pathways associated with among others adhesion, proliferation, Wnt-signaling, and placed ERβ in bipotential granulosa cell cluster. Loss of ERβ increased expression of the other estrogen receptors <em>Esr1</em> and <em>Gper1</em>.</div></div><div><h3>Conclusion(s)</h3><div>Our results show that ERβ has an important role in regulating ovulation in response to exogenous gonadotropins in 6-month-old mice, but not in younger mice. Our transcriptomic and immunohistochemical observations suggest a dysregulation of the granulosa cell communication and lack of tight coordination between granulosa cell replication and antrum expansion. A significant upregulation of other estrogen receptors may support a compensatory mechanism sustaining fertility during younger age in <em>Esr2</em>-KO mice.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"5 4","pages":"Pages 306-317"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tertiary lymphoid structures in endometriosis","authors":"Katherine B. Zutautas M.Sc. ,&nbsp;Priyanka Yolmo B.Tech. ,&nbsp;Minqi Xu M.D. ,&nbsp;Timothy Childs M.D. ,&nbsp;Madhuri Koti D.V.M., Ph.D. ,&nbsp;Chandrakant Tayade D.V.M., Ph.D.","doi":"10.1016/j.xfss.2024.10.001","DOIUrl":"10.1016/j.xfss.2024.10.001","url":null,"abstract":"<div><h3>Objective</h3><div>To determine whether tertiary lymphoid structures (TLSs), which reflect organized immune cell aggregates present in non-lymphoid tissues, are consistent features of endometriosis lesions.</div></div><div><h3>Design</h3><div>Detailed histopathological analysis of endometrial and lesion tissue from patients with endometriosis and controls was performed. Multiplex immunofluorescence on select samples was then conducted to identify canonical cell populations present within TLSs: CD3⁺ and CD8⁺ T-cells, CD79a⁺ B-cells, CD208⁺ dendritic cells, CD21⁺ follicular dendritic cells, and PNAd⁺ high endothelial venules.</div></div><div><h3>Patient(s)</h3><div>Patients with histologically confirmed endometriosis (N = 113; 44.3 ± 6.0) and control individuals (N = 110; 44.6 ± 7.1).</div></div><div><h3>Intervention</h3><div>Not applicable.</div></div><div><h3>Main Outcome Measure(s)</h3><div>Detection of TLSs as characterized by the presence of all canonical cell types that constitute TLS and structure morphology.</div></div><div><h3>Result(s)</h3><div>Of the selected samples (N = 18; 6 ectopic/eutopic/control), mature TLSs were identified in 3 ectopic tissue samples present on the ovary and fallopian tube, with immature TLSs (lacking follicular dendritic cell networks and high endothelial venules) present throughout eutopic and control endometrial samples.</div></div><div><h3>Conclusion</h3><div>These findings demonstrate the presence of TLSs across various endometriosis phenotypes, prompting further research into their significance within disease pathophysiology and the prognostic implications for patients.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"5 4","pages":"Pages 335-341"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142382619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From the Editor-in-Chief","authors":"William H. Catherino M.D., Ph.D.","doi":"10.1016/j.xfss.2024.10.006","DOIUrl":"10.1016/j.xfss.2024.10.006","url":null,"abstract":"","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"5 4","pages":"Pages 301-302"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Refining endometrial assembloids: a novel approach to 3-dimensional culture of the endometrium","authors":"Chloé Beaussart M.D. ,&nbsp;Margherita Rossi M.Sc. ,&nbsp;Christina Anna Stratopoulou Ph.D. ,&nbsp;Margherita Zipponi M.Sc. ,&nbsp;Luciana Cacciottola M.D., Ph.D. ,&nbsp;Jacques Donnez M.D., Ph.D. ,&nbsp;Marie-Madeleine Dolmans M.D., Ph.D.","doi":"10.1016/j.xfss.2024.08.005","DOIUrl":"10.1016/j.xfss.2024.08.005","url":null,"abstract":"","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"5 4","pages":"Pages 331-334"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum for Bhatt S, Butola A, Acuña S, Hansen DH, Tinguely JC, Nystad M, et al. Characterizing the consistency of motion of spermatozoa through nanoscale motion tracing. F S Sci 2024;5:215–24.","authors":"","doi":"10.1016/j.xfss.2024.09.001","DOIUrl":"10.1016/j.xfss.2024.09.001","url":null,"abstract":"","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"5 4","pages":"Page 404"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HMGA2 overexpression induces plasticity in myometrial cells and a transcriptomic profile more similar to that of uterine fibroids","authors":"Emmanuel N. Paul Ph.D.,&nbsp;Tyler J. Carpenter B.S.,&nbsp;Laura A. Pavliscak B.S.,&nbsp;Abigail Z. Bennett B.S.,&nbsp;Maria Ariadna Ochoa-Bernal Ph.D.,&nbsp;Asgerally T. Fazleabas Ph.D.,&nbsp;Jose M. Teixeira Ph.D.","doi":"10.1016/j.xfss.2024.07.006","DOIUrl":"10.1016/j.xfss.2024.07.006","url":null,"abstract":"<div><h3>Objective</h3><div>To study the possible role for <em>HMGA2</em> overexpression in differentiated myometrial cells and its potential to induce a stem cell-like or dedifferentiating phenotype and drive fibroid development.</div></div><div><h3>Design</h3><div>Myometrial cells were immortalized and transduced with an <em>HMGA2</em> lentivirus to produce HMGA2hi cells. In vitro stem cell assays were conducted, and ribonucleic acid from HMGA2hi and control cells as well as fibroid-free myometrial and <em>HMGA2</em> fibroid (HMGA2F) tissues were submitted for ribonucleic acid sequencing.</div></div><div><h3>Setting</h3><div>University research laboratory.</div></div><div><h3>Patient(s)</h3><div>Women who underwent hysterectomy for symptomatic uterine fibroids or other gynecological conditions.</div></div><div><h3>Intervention(s)</h3><div>Not applicable.</div></div><div><h3>Main Outcome Measure(s)</h3><div>In vitro stem cell-like properties from myometrial cell lines. Ribonucleic acid sequencing and collagen production of <em>HMGA2</em>-overexpressing primary leiomyoma tissue and cell lines.</div></div><div><h3>Result(s)</h3><div>HMGA2hi cells had enhanced self-renewal capacity, decreased proliferation, and a greater ability to differentiate into other mesenchymal cell types. HMGA2hi cells exhibited a stem cell-like signature and shared transcriptomic similarities with HMGA2F. Moreover, dysregulated extracellular matrix pathways were observed in both HMGA2hi cells and HMGA2F.</div></div><div><h3>Conclusion(s)</h3><div>Our findings show that <em>HMGA2</em> overexpression may drive myometrial cells to dedifferentiate into a more plastic phenotype and provide evidence for an alternative mechanism for fibroid etiology, suggesting that fibroids arise not only from a mutated stem cell but also from a mutated differentiated myometrial cell.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"5 4","pages":"Pages 369-378"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141705225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coenzyme Q-10 reduced the aberrant production of extracellular matrix proteins in uterine leiomyomas through transforming growth factor beta 3","authors":"Charlene Echague D.O. ,&nbsp;Minnie Malik Ph.D. ,&nbsp;Paul Driggers Ph.D. ,&nbsp;William H. Catherino M.D., Ph.D.","doi":"10.1016/j.xfss.2024.07.004","DOIUrl":"10.1016/j.xfss.2024.07.004","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the impact of coenzyme Q-10 (CoQ-10) on the dysregulated synthesis of extracellular matrix proteins mediated by transforming growth factor beta 3 (TGF-β3) in uterine leiomyomas.</div></div><div><h3>Design</h3><div>Laboratory study.</div></div><div><h3>Setting</h3><div>University.</div></div><div><h3>Patients</h3><div>None.</div></div><div><h3>Interventions</h3><div>Treatment of immortalized uterine myometrial and leiomyoma cells to TGF-β3 and CoQ-10.</div></div><div><h3>Main Outcome Measures</h3><div>The protein concentrations of collagen 1A1 (COL1A1), collagen 3A1 (COL3A1), collagen 11A1 (COL11A1), and fibronectin (FN1) were assessed through western blot analysis after treatment of immortalized uterine myometrial and leiomyoma cells with both transforming growth factor beta (TGF-β) 3 and concentrations of CoQ-10 at 10, 50, and 100 μM concurrently for 24 hours.</div></div><div><h3>Results</h3><div>Immortalized uterine leiomyoma and myometrial cells exposed to TGF-β3 for 24 hours demonstrated a significant up-regulation of COL1A1, COL3A1, COL11A1, and FN1 compared with untreated cells. In leiomyoma cells, concurrent treatment with CoQ-10 over the same timeframe revealed a dose-dependent decrease in these protein concentrations compared with those in cells treated with TGF-β3 alone. At the highest concentration of 100 μM of CoQ-10, significant decreases in the amounts of COL1A1 (0.59 ± 0.10-fold), COL3A1 (0.46 ± 0.09-fold), COL11A1 (0.53 ± 0.09-fold), and FN1 (0.56 ± 0.09-fold) were observed. Similarly, myometrial cells exposed to both TGF-β3 and CoQ-10 demonstrated a dose-responsive decline in the amount of extracellular matrix protein compared with cells exposed to TGF-β3 alone. Significant reductions in the amounts of COL1A1 (0.75 ± 0.03-fold), COL3A1 (0.48 ± 0.06-fold), COL11A1 (0.38 ± 0.06), and FN1 (0.69 ± 0.04-fold) were appreciated at 100-μM CoQ-10.</div></div><div><h3>Conclusion</h3><div>Coenzyme Q-10 mitigated the aberrant production of key biomarkers of the extracellular matrix mediated by TGF-β3 in uterine leiomyomas. Our findings highlight a promising nonhormonal compound that can counteract the fibroproliferative process inherent to leiomyomas.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"5 4","pages":"Pages 342-351"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skoochies and its component substances induced testicular damage and impaired sperm function via increased generation of reactive oxygen species and impairment of the glutathione system in rats","authors":"Ayodeji Folorunsho Ajayi Ph.D. ,&nbsp;Olufemi Ogundeji Ogundipe M.Tech. ,&nbsp;Moses Agbomhere Hamed B.M.L.S. ,&nbsp;David Tolulope Oluwole M.Phil.","doi":"10.1016/j.xfss.2024.07.005","DOIUrl":"10.1016/j.xfss.2024.07.005","url":null,"abstract":"<div><h3>Objective</h3><div>To examine the effect of skoochies, an illicit cocktail drink, on testicular and sperm function in male rats.</div></div><div><h3>Design</h3><div>Twenty-five adult male Wistar rats were assigned randomly into five groups (n = 5) as follows: normal saline; skoochies; <em>Cannabis sativa</em>; codeine; and tramadol. The cocktail (skoochies) used in this study was formulated with the following composition: codeine (5 mg/kg); tramadol (20 mg/kg); and cannabis extract (2 mg/kg). These doses are as previously reported. Administration was performed once daily for 28 days.</div></div><div><h3>Setting</h3><div>University.</div></div><div><h3>Animal(s)</h3><div>Twenty-five (25) male Wistar rats.</div></div><div><h3>Intervention(s)</h3><div>Skoochies, tramadol, Codeiene, Cannabis.</div></div><div><h3>Main Outcome Measure(s)</h3><div>Skoochies and its components induced testicular and sperm damage via increased generation of reactive oxygen species and impairment of glutathione system in rats.</div></div><div><h3>Result(s)</h3><div>Skoochies increased reactive oxygen species generation and impaired the antioxidant system resulting in inflammation that eventually damaged the testicular tissue. Skoochies caused oxidoinflammatory injury to this tissue, leading to impaired testicular function. This was evident by the distorted cytoarchitecture, reduced sperm count and motility, and impaired testicular deoxyribonucleic acid integrity.</div></div><div><h3>Conclusion(s)</h3><div>Thus, our results infer that skoochies impaired the testicular and sperm function through the increased generation of reactive oxygen species and impairment of the glutathione system.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"5 4","pages":"Pages 318-330"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prostaglandin E2 regulates the plasminogen activator pathway in human endometrial endothelial cells: a new in vitro model to investigate heavy menstrual bleeding","authors":"Seifeldin Sadek M.D. ,&nbsp;Terry A. Jacot Ph.D. ,&nbsp;Diane M. Duffy Ph.D. ,&nbsp;David F. Archer M.D.","doi":"10.1016/j.xfss.2024.07.007","DOIUrl":"10.1016/j.xfss.2024.07.007","url":null,"abstract":"<div><h3>Objective</h3><div>To study the role of PGE₂ in regulating plasminogen activator inhibitor-1 (PAI-1) and tissue plasminogen activator (tPA) in human primary endometrial endothelial cells (HEECs) from women with normal menstrual bleeding (NMB) and heavy menstrual bleeding (HMB).</div></div><div><h3>Design</h3><div>In vitro study using endometrial endothelial cells.</div></div><div><h3>Setting</h3><div>Research laboratory setting.</div></div><div><h3>Patients</h3><div>Women with NMB and HMB provided endometrial biopsy samples.</div></div><div><h3>Interventions</h3><div>Prostaglandin E₂ and PGE₂ receptor-selective agonists were administered to cultured HEECs.</div></div><div><h3>Main Outcome Measures</h3><div>Levels of PAI-1 and tPA in NMB-HEECs and HMB-HEECs after treatment with PGE₂ and receptor-selective agonists.</div></div><div><h3>Results</h3><div>Prostaglandin E₂ increased total PAI-1 levels in NMB-HEECs, but not in HMB-HEECs, which had higher baseline PAI-1 levels. PGE₂ receptors (PTGER)1 and PTGER2 agonists increased PAI-1 in NMB-HEECs, whereas PTGER3 and PTGER4 did not. Prostaglandin E₂ had no effect on tPA levels in either NMB-HEECs or HMB-HEECs.</div></div><div><h3>Conclusions</h3><div>Prostaglandin E₂, through PTGER1 and PTGER2, regulates the plasminogen activator system in NMB-HEECs, suggesting a role in reducing fibrinolytic activity during normal menstrual cycles. The lack of PGE₂ effect and elevated baseline PAI-1 in HMB-HEECs support using this in vitro model to further understand prostaglandin pathways in NMB and HMB.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"5 4","pages":"Pages 379-385"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141749911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}