F&S science最新文献

{"title":"Reviewer of the Year 2024. F&S Science celebrates excellence in our world-class reviewers.","authors":"","doi":"10.1016/j.xfss.2025.07.001","DOIUrl":"https://doi.org/10.1016/j.xfss.2025.07.001","url":null,"abstract":"","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144710109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compressive Force Induces Differential Gene and Protein Expression in Uterine Fibroids.","authors":"Carolyn A Nietupski, Megan R Sax, Rose Dean, Andreja Moset Zupan, Emily G Hurley, Stacey C Schutte","doi":"10.1016/j.xfss.2025.07.004","DOIUrl":"https://doi.org/10.1016/j.xfss.2025.07.004","url":null,"abstract":"<p><strong>Objective: </strong>To study how compressive forces influence fibroid and myometrial cells. Our work aimed to identify proteins and signaling pathways that are altered in fibroids in response to compressive forces.</p><p><strong>Design: </strong>Laboratory-based SUBJECTS: Patient-matched fibroid and myometrial cells were isolated from 5 women undergoing hysterectomy or myomectomy for the treatment of uterine fibroids. Only samples from women who had not had hormonal modulation within three months of surgery were used for this study. An embedded spheroid model was developed to model the fibroid tissue and provide a cushion that would help with the distribution of compressive force.</p><p><strong>Exposure: </strong>Weights, 0 or 6.4 mmHg, were added on top of an agarose cushion. Spheroids were cultured for seven days.</p><p><strong>Main outcome measures: </strong>Histological evaluation, RNA-sequencing (n=5), and proteomics characterization (n=3). Paired multi-test t-tests were performed for statistical analysis. Differentially expressed genes (DEGs) were considered clinically relevant if the same genes were also significantly differentially expressed in at least one of the four existing fibroid and myometrium RNA-sequencing datasets.</p><p><strong>Results: </strong>A total of 61 clinically relevant DEGs were identified between cell types that were only differentially expressed when the spheroids were under compression. This included EPHB1 which encodes ephrin signaling receptor EphB1; it was upregulated log2 fold-change of 2.81 in fibroid cells (q=5.35×10^-3). Compression led to the enrichment of genes involved in extracellular matrix (ECM) organization; however, the genes varied between the cell types. At the protein level, myometrial spheroids had alterations in proteins associated with uterine fibroids (q=1.00x10^-33). There were alterations in collagen abundance in fibroid spheroids, but not collagen 1 although the collagenase MMP-1 was significantly lower in fibroid spheroids. Enrichment analysis identified ECM-receptor interactions as enriched in compression-induced changes between the cell types.</p><p><strong>Conclusions: </strong>Compressive forces must be considered to study some of the important differences between fibroids and myometrium, including ephrin signaling. Enrichment analysis of the proteins with different abundances suggests that compression may also be involved in fibroid tumor initiation.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144710108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence-driven oocyte assessment for predicting blastulation and high-quality blastocyst formation in severe male factor infertility.","authors":"Edson Borges, Daniela Braga, Maite Del Collado, Assumpto Iaconelli, Jullin Fjeldstad, Natalie Mercuri, Parisa Mojiri, Amanda Setti","doi":"10.1016/j.xfss.2025.07.003","DOIUrl":"https://doi.org/10.1016/j.xfss.2025.07.003","url":null,"abstract":"<p><strong>Objective: </strong>To study whether AI-driven oocyte evaluation is associated with blastocyst development and quality in couples with severe male factor infertility (SMF) undergoing intracytoplasmic sperm injection (ICSI) cycles.</p><p><strong>Design: </strong>Cohort study SUBJECTS: 14,602 oocyte images from 2,156 ICSI cycles performed between January 2020 and May 2024 in a private university-affiliated in vitro fertilization center. Cycles were categorized into two groups: SMF (n=200 cycles, 1,478 embryos) and non-SMF (n=1,956 cycles, 13,124 embryos). SMF was defined as <5 million sperm in the ejaculate.</p><p><strong>Exposure: </strong>Oocyte images were captured before ICSI and scored using the AI tool MAGENTA™. The predictive value of Magenta Scores (MS) on embryonic development was assessed. The association between MS and oocyte fertilization and blastocyst formation was analyzed.</p><p><strong>Main outcome measure(s): </strong>Oocyte fertilization, blastulation rate, blastocyst quality.</p><p><strong>Results: </strong>MS were significantly lower in oocytes that failed to fertilize compared to those that successfully fertilized (5.00 ± 0.04 vs. 6.44 ± 0.03, p<0.001). Blastulation rate was lower in the SMF group (46.61% vs. 50.80%, p=0.003), and blastocysts exhibited higher MS than non-blastocysts (5.12 ± 0.3 vs. 6.69 ± 0.3, p<0.001). The top-quality blastocyst rate was lower in SMF (56.6% vs. 65.2%, p<0.001), and high-quality blastocysts had higher MS than lower-quality ones (7.2 ± 0.6 vs. 6.8 ± 0.5, p<0.001). Among SMF cycles, MS were lower in oocytes that failed to fertilize (4.91 ± 0.12 vs. 6.34 ± 0.10, p<0.001). MS also differed between embryos that reached the blastocyst stage and those that did not (6.70 ± 0.11 vs. 4.96 ± 0.10, p<0.001). Top-quality blastocysts had significantly higher MS than others (7.00 ± 0.21 vs. 6.39 ± 0.19, p<0.001). Paternal age negatively correlated with fertilization, blastulation, and blastocyst quality; however, differences remained significant after adjusting for paternal age.</p><p><strong>Conclusion: </strong>AI-based oocyte evaluation is associated with fertilization, blastulation, and blastocyst quality in SMF couples undergoing ICSI cycles. MS values were consistently higher for blastocysts than non-blastocysts, demonstrating the AI tool's utility in identifying oocytes with greater developmental potential, regardless of male infertility factors. However, the absence of sperm-specific factors in the MAGENTA™ algorithm may limit its ability to fully account for male infertility.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144661170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From the Editor-in-Chief.","authors":"William H Catherino","doi":"10.1016/j.xfss.2025.07.002","DOIUrl":"10.1016/j.xfss.2025.07.002","url":null,"abstract":"","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144661171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between early and mid-lateal phase progesterone levels and the DNA methylation of WOI-related genes in cervical secretions in fresh and frozen IVF cycles.","authors":"Cemil Kaya, Kübra Nur Kaplan İlhan, Bala Gur Dedeoglu, Mehmet Erdem, Ahmet Erdem","doi":"10.1016/j.xfss.2025.06.001","DOIUrl":"https://doi.org/10.1016/j.xfss.2025.06.001","url":null,"abstract":"<p><strong>Objective: </strong>To study the association between serum progesterone levels on the day of embryo transfer and the DNA methylation of window of implantation (WOI)-related genes in cervical secretions, in both fresh and frozen embryo transfer cycles among women undergoing in vitro fertilization (IVF) treatment.</p><p><strong>Design: </strong>Single-centre retrospective cohort study SUBJECTS: Women undergoing embryo transfer cyles.</p><p><strong>Exposure: </strong>Cervicovaginal materials have been used as a noninvasive surrogate in investigations of endometrial conditions and progesteron levels on day embryo transfer.</p><p><strong>Main outcome measure(s): </strong>Progesterone levels, methylation changes, RESULTS: A total of 40 eligible women undergoing in vitro fertilization (IVF) embryo transfer cycles were evaluated. Based on methylation analyses of CXCL14, EMCN, RARRES1, PAEP, THBS2, and GPX3, no statistically significant correlation was found with median serum progesterone (P4) levels on the day of embryo transfer (p > 0.05). When participants were grouped into quartiles based on P4 levels on the day of embryo transfer, no statistically significant differences were observed in the methylation levels of CXCL14, SERPING1, EMCN, RARRES1, PAEP, THBS2, and GPX3 (p > 0.05 for all comparisons). Median P4 levels were significantly lower in the thawed embryo transfer group (median: 21.4 ng/mL; interquartile range [IQR]: 13.3-24.0) compared to the fresh transfer group (median: 24.0 ng/mL; IQR: 18.0-45.0) (p = 0.039). No significant differences in gene methylation levels were observed between fresh and thawed transfer types, except for THBS2, which showed significantly lower methylation levels in the thawed group compared to the fresh group (p = 0.013). Although median P4 levels were lower on day 3 transfers (16.6 ng/mL) compared to day 5 transfers (22.4 ng/mL), the difference was not statistically significant (p > 0.05). Similarly, no significant differences in methylation levels were detected when comparing day 3 vs. day 5 embryo transfer groups.</p><p><strong>Conclusions: </strong>There is no association was found between early and mid-luteal phase progesterone levels and methylation changes of implantation-related genes in cervical secretions during both fresh and frozen IVF cycles.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome landscapes induced by estetrol, estradiol, and ethinylestradiol in primary human endometriotic stromal cells.","authors":"Marie Nakajima, Gentaro Izumi, Kaori Koga, Vita Silvana, Mohammed Elsherbini, Atsushi Okumura, Michihito Wada, Satoru Yamanaka, Yasushi Hirota, Yutaka Osuga","doi":"10.1016/j.xfss.2025.06.002","DOIUrl":"10.1016/j.xfss.2025.06.002","url":null,"abstract":"<p><strong>Objective: </strong>To compare the transcriptomes induced by estrogens used in combined oral contraceptives (COCs): estetrol (E4), ethinylestradiol (EE), and estradiol (E2) in human primary endometriotic stromal cells.</p><p><strong>Design: </strong>Endometriotic stromal cells were cultured in the presence of E4 (10^-6 mol/L), E2 (10^-8 mol/L) or EE (10^-9 mol/L) for 24 hours. Transcriptomes induced by E4 were compared with those induced by E2 and EE.</p><p><strong>Subjects: </strong>Endometriotic stromal cells were obtained from patients with ovarian endometriomas.</p><p><strong>Main outcome measures: </strong>Transcriptomes were examined using RNA sequencing analysis, and messenger RNA levels were measured using reverse transcription-quantitative polymerase chain reaction.</p><p><strong>Results: </strong>Estetrol treatment resulted in 114 differentially expressed genes compared with the control (|log2fold change| >0.2). The number was higher than those differentially expressed between E4 and E2 (82 genes) and between E4 and EE (75 genes). Of the differentially expressed genes between E4 and the control, 79% (90 genes) changed in the same manner as in EE and E2. In contrast, 21% (24 genes) of the genes changed in an E4-preferential manner.</p><p><strong>Conclusion: </strong>The present study elucidated that although E4 induces the expression of specific unique genes in endometriotic stromal cells distinct from those regulated by other estrogens, the overall gene expression profile is largely analogous to that induced by other estrogens. When differences in progestin are not considered, it is suggested that the COC containing E4 exhibits effects on endometriosis comparable with those of other COCs.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of a microfluidic-based selection device on sperm deoxyribonucleic acid fragmentation and intracytoplasmic sperm injection cycles.","authors":"Maria Luisa Pardiñas, Rocío Rivera-Egea, Jose Maria de Los Santos, Carmen Vidal, Juan Giles, David Ortega-Jaen, Julia Gil, Angel Martin, Thamara Viloria, Maria Jose de Los Santos","doi":"10.1016/j.xfss.2025.05.002","DOIUrl":"10.1016/j.xfss.2025.05.002","url":null,"abstract":"<p><strong>Objective: </strong>To determine the impact of a microfluidic-based sperm selection device on sperm parameters and embryo variables compared with the conventional swim-up method in sibling oocytes.</p><p><strong>Design: </strong>Prospective observational study.</p><p><strong>Subjects: </strong>A total of 345 oocytes were recruited from 27 couples undergoing intracytoplasmic sperm injection, including both own (n = 195) and donation (n = 150) cycles. None of the patients presented severe male factor.</p><p><strong>Intervention/exposure: </strong>Semen sample was divided into 2 groups and processed using swim-up or the microfluidic sperm selection device (MSSD) ZyMōt. Half of the oocytes were inseminated with swim-up-selected spermatozoa, and the rest were inseminated with MSSD-selected spermatozoa. Embryo development was followed by time-lapse. Sperm deoxyribonucleic acid (DNA) fragmentation was measured using the sperm chromatin dispersion test, analyzed with ImageJ. A Mann-Whitney U test was performed for statistical analysis.</p><p><strong>Main outcome measures: </strong>Sperm DNA fragmentation levels, sperm parameters, fertilization rates, embryo morphokinetics, and rate of usable blastocysts.</p><p><strong>Results: </strong>Sperm DNA fragmentation was significantly lower in the MSSD group than in the swim-up group (20.3% vs. 11%), indicating a better selection. Analyzing separately the oocytes from the patient's own cycles, MSSD showed a significantly higher rate of usable blastocysts per fertilized oocyte. However, this difference was not observed using donated oocytes or when both cycles were combined. Embryos from the swim-up group showed a significant delay in time of pronuclear appearance and morula formation compared with those from the MSSD group, being more marked in donated oocytes. No significant differences were observed in the fertilization rate and the remaining morphokinetic times.</p><p><strong>Conclusion: </strong>This study provides valuable information on the use of MSSD for noninvasive sperm selection. When MSSD was used, we observed a reduction in sperm DNA fragmentation and an enhancement in the number of usable embryos in our own cycles. These findings could be compatible with a reduced capacity to repair sperm damage due to poorer oocyte quality caused by advanced age.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic expression of endometrial adhesion G protein-coupled receptors during the menstrual cycle and early mouse pregnancy: modulation by ovarian stimulation.","authors":"Nischelle Kalakota, Alexander Lemenze, Lea George, Qingshi Zhao, Tracy Wu, Sara S Morelli, Andy V Babwah, Nataki C Douglas","doi":"10.1016/j.xfss.2025.05.001","DOIUrl":"10.1016/j.xfss.2025.05.001","url":null,"abstract":"<p><strong>Objective: </strong>To characterize the expression of adhesion G protein-coupled receptors (ADGR) in the human endometrium and early mouse pregnancy.</p><p><strong>Design: </strong>An in silico analysis was performed using a retrospective data set comprised endometrial samples across normo-ovulatory menstrual cycles. Gene expression was then validated using quantitative reverse transcription polymerase chain reaction and mRNA sequencing (mRNA-seq) in prospectively collected endometrial biopsies in the periovulatory and midsecretory stages of natural cycles. Gene expression was also investigated under ovarian stimulation (OS) conditions using mRNA-seq. Early pregnancy mouse models were used to investigate whether trends of dynamic ADGR expression are also conserved in the mouse.</p><p><strong>Subjects: </strong>Twenty-four women aged 21-42 years.</p><p><strong>Exposure: </strong>Ovulatory menstrual cycle or OS cycle.</p><p><strong>Main outcome measures: </strong>Gene expression in endometrial biopsies and pregnant mouse uterus.</p><p><strong>Results: </strong>Fifteen women, aged 21-33 years, were recruited in natural cycles during the proliferative phase (cycle days 10-13; n = 4), periovulatory (luteinizing hormone + 12-24 hours; n = 6) period, and midsecretory (luteinizing hormone + 8-9 days; n = 5) phase. Nine women aged 31-42 years old undergoing in vitro fertilization (without fresh embryo transfer) or oocyte cryopreservation using a gonadotropin releasing hormone antagonist protocol were recruited for the OS cohort in either the periovulatory phase (human chorionic gonadotropin + 2; n = 5) or midsecretory phase (human chorionic gonadotropin + 9; n = 4). The in silico analysis revealed dynamic expression for many ADGRs across the menstrual cycle. Differential gene expression was also seen in the prospective analysis within the menstrual cycle phases and between natural cycle and OS conditions. Within early mouse pregnancy, expression was also found to be altered across several Adgr subfamilies.</p><p><strong>Conclusion: </strong>The differential gene expression observed between the proliferative and secretory phases of the menstrual cycle, along with changes in expression seen in OS and early mouse pregnancy suggest that ADGR expression is hormonally regulated by estradiol and progesterone.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A sound approach for ova denudation","authors":"Amir Mokhtare Ph.D. ,&nbsp;Amirhossein Favakeh M.S. ,&nbsp;Philip Xie B.Sc. ,&nbsp;Zev Rosenwaks M.D. ,&nbsp;Alireza Abbaspourrad Ph.D. ,&nbsp;Gianpiero Palermo M.D., Ph.D.","doi":"10.1016/j.xfss.2024.12.005","DOIUrl":"10.1016/j.xfss.2024.12.005","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;div&gt;To introduce an innovative noncontact method for denudation process of cumulus-oocyte complexes (COCs) for intracytoplasmic sperm injection (ICSI).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Design&lt;/h3&gt;&lt;div&gt;We designed and fabricated novel acoustohydrodynamic tweezers (AHTs) to perform contactless denudation and tested them in a mouse model. Cumulus removal efficiency, preimplantation development, and live birth were assessed and compared with those in conventional manual pipetting (MP) denudation.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Subjects&lt;/h3&gt;&lt;div&gt;Fourteen female B6D2F1/J mice (approximately 4 weeks of age), nine male B6D2F1/J mice (6–12 weeks of age), and 28 CD-1 female mice (approximately 6 weeks of age) were used for experiment.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Exposure&lt;/h3&gt;&lt;div&gt;We designed a contactless platform for oocyte denudation on the basis of the principle of focalized acoustic waves. We first investigated the acoustic intensity and thermal variability by measuring the surface displacement and temperature with a thermal camera to ensure a safe operation. Cumulus-oocyte complexes were denuded by conventional MP with 40 IU/mL of hyaluronidase serving as control or by AHTs with a reduced amount of hyaluronidase (15 IU/mL). Piezo-ICSI was performed on both experimental and control groups. A triplicate of denudation and insemination experiments was performed. All embryos were monitored in a time-lapse incubator. Embryo developmental rates were compared by the chi-square test. Embryo morphokinetic timing as a continuous variable was compared by 1-way analysis of variance. Embryo transfers were performed on some blastocysts.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Main Outcome Measures&lt;/h3&gt;&lt;div&gt;The procedural time for each denudation method was measured and compared. Fertilization, embryo development and morphokinetics, pregnancy, and live birth rate were compared between the control and experimental cohorts.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Facile noncontact denudation was achieved without any damage to oocyte. Acoustic induced fluidic shear was the main contributor to COC denudation. The average denudation time per oocyte decreased by 46% (15 seconds per oocyte for control vs. 8 seconds per oocyte for AHT) while using a lower concentration of hyaluronidase. Piezo-ICSI on oocytes processed by MP and AHTs resulted in comparable rates of survival (86.1% vs. 85.3%), fertilization (96.7% vs. 94.1%), and blastocyst (88.0% vs. 81.3%). Embryo morphokinetics for both experimental and control cohorts were comparable, showing no impact of sound waves on the embryo development. Eventual delivery rates were also comparable between the MP and AHT cohorts (51.3% vs. 55.4%).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;Acoustohydrodynamic tweezers are used for contactless removal of the cumulus cells from the COCs before ICSI in an expedited, safe, and reliable manner. Embryo development outcomes confirm their safety and validate their potential for a comprehensive ICSI-on-chip device.&lt;/div&gt;&lt;/div","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"6 2","pages":"Pages 118-125"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luteinizing hormone receptor deficiency in immature cumulus-oocyte complexes retrieved for assisted reproduction","authors":"Maíra Casalechi Ph.D.,&nbsp;Maria Thereza V. Pereira M.Sc.,&nbsp;Wiviane A. Assis Ph.D.,&nbsp;Cynthia Dela Cruz Ph.D.,&nbsp;Tays F. Guedes B.Sc.,&nbsp;Ines Katerina Cavallo M.D., Ph.D.,&nbsp;Fernando M. Reis M.D., Ph.D.","doi":"10.1016/j.xfss.2024.12.006","DOIUrl":"10.1016/j.xfss.2024.12.006","url":null,"abstract":"<div><div>This study investigated whether luteinizing hormone receptor (LHR) expression varies in the granulosa cells of individual follicles according to the maturation stage of the oocytes harvested for assisted reproductive technology treatment. We observed minimal to no LHR messenger ribonucleic acid and protein expression in cumulus cells surrounding oocytes arrested in the germinal vesicle stage. Interestingly, their ability to mature was confirmed by rescue in vitro maturation, suggesting somatic cell LHR deficiency as a key factor for the retrieval of germinal vesicle oocytes in assisted reproductive technology procedures.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"6 2","pages":"Pages 126-129"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}