F&S science最新文献

{"title":"Comparison of hysterosalpingo-foam sonography vs. hysterosalpingography: a prospective observational study.","authors":"Pablo Luque González, Ángela Morales Bueno, Inmaculada Del Río Romero, José Luis Dueñas Díez, María Del Pilar Guadix Martín","doi":"10.1016/j.xfss.2026.02.003","DOIUrl":"10.1016/j.xfss.2026.02.003","url":null,"abstract":"<p><strong>Objective: </strong>To study the diagnostic concordance and tolerability of hysterosalpingo-foam sonography (HyFoSy) compared with hysterosalpingography (HSG) in the evaluation of tubal patency.</p><p><strong>Design: </strong>Prospective observational study conducted between 2023 and 2024 in a Spanish hospital, using a within-subject comparative design.</p><p><strong>Subjects: </strong>A total of 117 women undergoing fertility evaluation.</p><p><strong>Exposure: </strong>All participants underwent both HyFoSy and HSG for assessment of tubal patency.</p><p><strong>Main outcome measures: </strong>Diagnostic concordance between HyFoSy and HSG (Cohen's kappa coefficient), pain assessment using the visual analogue scale (VAS), and procedure-related complications.</p><p><strong>Results: </strong>Complete concordance between HyFoSy and hysterosalpingography was obtained in 100 patients (85.4%), of whom 85 had normal patency, and 15 had some patency defect (moderate agreement with a kappa coefficient of 0.575). In 12 patients (10.2%), there were discordant diagnoses, and in five patients (4.3%), concordance could not be assessed. Hysterosalpingo-foam sonography was better tolerated in terms of reported pain, while HSG had a significantly higher median pain score. Only minor complications were recorded during the performance of both techniques, mainly slight vaginal bleeding and vasovagal discomfort.</p><p><strong>Conclusions: </strong>Our study shows HyFoSy is an effective, safe and better-tolerated technique than HSG, with a similar diagnostic rate for the study of tubal patency defects.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147370825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stereological assessment of resveratrol's impact on folliculogenesis and sex hormone levels in busulfan-treated rats.","authors":"Negin Rafati Nia, Morteza Golbashirzadeh, Atousa Moradzadegan","doi":"10.1016/j.xfss.2026.02.002","DOIUrl":"10.1016/j.xfss.2026.02.002","url":null,"abstract":"<p><strong>Objective: </strong>To study the stereological effects of resveratrol on folliculogenesis and sex hormone concentrations in busulfan-treated rats.</p><p><strong>Design: </strong>Experimental animal study.</p><p><strong>Subjects: </strong>Twenty-four adult female Sprague-Dawley rats.</p><p><strong>Exposure: </strong>Animals were randomly assigned to 4 groups: control, busulfan (10 mg/kg, single intraperitoneal dose), busulfan plus resveratrol (10 mg/kg), and busulfan plus resveratrol (20 mg/kg). Resveratrol was administered orally for 35 days.</p><p><strong>Main outcome measures: </strong>Serum concentrations of luteinizing hormone, follicle-stimulating hormone, estradiol, and progesterone were measured. Ovarian stereological analysis assessed follicle counts and corpus luteum volume.</p><p><strong>Results: </strong>Busulfan exposure caused severe ovarian damage, including reduced estradiol and progesterone levels, diminished corpus luteum volume, and increased gonadotropins and atretic follicles. High-dose resveratrol (20 mg/kg) partially improved estradiol and progesterone concentrations, increased healthy follicle counts, and enhanced corpus luteum volume, while reducing gonadotropins and atretic follicles (P<.05). These findings indicate attenuation but not complete restoration of busulfan-induced ovarian dysfunction.</p><p><strong>Conclusion: </strong>Busulfan disrupts ovarian function and impairs folliculogenesis. Resveratrol partially mitigates these adverse effects, suggesting its potential as a protective agent against chemotherapy-associated reproductive toxicity.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147357828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of oxidative stress parameters in follicular fluids between patients with unexplained infertility or diminished ovarian reserve: a prospective cohort study","authors":"Elif Sagban Gedik M.D. ,&nbsp;Mustafa Can Sivas M.D. ,&nbsp;Ibrahim Polat M.D.","doi":"10.1016/j.xfss.2025.12.004","DOIUrl":"10.1016/j.xfss.2025.12.004","url":null,"abstract":"<div><h3>Objective</h3><div>To study the levels of oxidant and antioxidant parameters, including total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI), in the follicular fluid of patients with diminished ovarian reserve (DOR) or unexplained infertility (UEI), to compare these groups, and to analyze the relationship between these parameters and antimüllerian hormone (AMH) levels.</div></div><div><h3>Design</h3><div>The study was planned and completed as a prospective cohort study.</div></div><div><h3>Subjects</h3><div>A total of 90 patients (n [DOR] = 45 and n [UEI] = 45) aged 23–38 years, who applied to undergo in vitro fertilization treatment between February 2024 and August 2024 and were diagnosed with DOR or UEI, were included in the study. Total oxidant status and TAS levels were determined in follicle fluid obtained during oocyte retrieval via double-antibody sandwich enzyme-linked immunosorbent assay.</div></div><div><h3>Exposure</h3><div>Patients categorized as having DOR or UEI.</div></div><div><h3>Main Outcome Measures</h3><div>Oocyte retrieval was performed on all patients, and follicular fluid was obtained simultaneously. Total antioxidant status and TOS values were measured in the follicular fluid, and the OSI value was calculated. Total antioxidant status, TOS, and OSI values were compared between the groups. The relationship between the three parameters and AMH levels was analyzed separately in each group. To analyze the relationship between low OSI values and AMH levels, mean OSI values within the group were determined. The AMH levels of the populations above and below the mean OSI value within the same group were compared.</div></div><div><h3>Results</h3><div>No significant differences in TAS, TOS, and OSI values were observed between groups. A significant positive correlation was found between TOS values and AMH levels in the DOR group. A statistically significant difference was found between AMH levels of the populations with OSI values above or below the mean in the UEI group. Antimüllerian hormone levels of the population with lower OSI values were higher. A high TOS value may not indicate that the AMH level is negatively affected. The AMH level may be negatively affected at high OSI values, indicating increased oxidative stress levels.</div></div><div><h3>Conclusion</h3><div>In evaluating the relationship between the oxidant-antioxidant system and infertility, it may not be appropriate to comment on the TOS or TAS value alone. The OSI value may significantly influence the AMH level.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"7 2","pages":"Pages 163-171"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145727590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANA-12 restores testicular health by local neurochemicals in passive smoking/nicotine-exposed preclinical adult rats","authors":"Deepsi Rathore M.Sc. ,&nbsp;Vishakha Shrimali M.Sc. ,&nbsp;Nibedita Naha Ph.D. ,&nbsp;Vijay Kumar Shivgotra Ph.D.","doi":"10.1016/j.xfss.2025.11.003","DOIUrl":"10.1016/j.xfss.2025.11.003","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the therapeutic potential of ANA-12 in restoring testicular health by modulating local neurochemical balance in adult rats of passive smoking, secondhand smoking or environmental tobacco smoke/nicotine exposure.</div></div><div><h3>Design</h3><div>Preclinical rodent models mimicking a real-life human smoking scenario.</div></div><div><h3>Subjects</h3><div>Post-acclimatization, adult male rats of 250–300 g body weight were randomized into passive smoking, oral nicotine, ANA-12 pre-treatment, and healthy unexposed controls.</div></div><div><h3>Exposure</h3><div>Rats were exposed to passive smoking through a whole-body inhalation chamber and oral nicotine through gavage with/without intraperitoneal administration of ANA-12 at differential dosages prior to passive smoking/nicotine exposure for a period of 4- and 12-week studies.</div></div><div><h3>Main Outcome Measure(s)</h3><div>Testes histopathology, DNA damage potency, and fertility indices alongside redox homeostasis and expressions of local neurotransmitter dopamine and its receptors D1 and D2, and neurotrophic factor, brain-derived neurotrophic factor (BDNF) and its receptor, tyrosine kinase beta (Trk-β) in testes, comet assay in whole blood, and serum cotinine levels.</div></div><div><h3>Result(s)</h3><div>Compared with the healthy unexposed controls, passive smoking and oral nicotine exposure dose- and time-dependently disrupt developing spermatogonia, spermatocytes, and spermatids of the seminiferous tubules and basement membrane; reduce sperm count with concomitant higher deoxyribonucleic acid (DNA) damage; and upregulate BDNF/Trk-β and dopamine/D1 expressions, as well as oxidative stress with subsequent antioxidant depletion in testes. In contrast, regardless of the duration of exposure, ANA-12 pre-treatment significantly restores germ cells of the seminiferous epithelium, improves spermatogenesis, downregulates aberrant local neurochemical systems, and maintains redox homeostasis in the testicular milieu. These results are in accordance with the whole blood DNA damage and serum cotinine levels, a biomarker of nicotine exposure.</div></div><div><h3>Conclusion(s)</h3><div>The results offer a novel insight into therapeutic interventions for passive or secondhand smoking- or environmental tobacco smoke-induced male reproductive impairment via regulation of BDNF-dopaminergic signaling and antioxidant balance in testes by ANA-12, leading to improved fertility potential and overall testicular health, which could establish the modulation of the brain-testes axis, implicated in nicotine addiction. This study holds translational potential of ANA-12 for the management of smoking- or nicotine-related male infertility of individuals unable or unwilling to quit smoking.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"7 2","pages":"Pages 124-137"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146013527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Angiotensin II drives proliferation and extracellular matrix deposition in human uterine fibroid cells in vitro","authors":"Abigail Lepsch Combs M.D. ,&nbsp;Gregory W. Kirschen M.D., Ph.D. ,&nbsp;Malak El Sabeh M.D. ,&nbsp;Sadia Afrin Ph.D. ,&nbsp;Mariko Miyashita-Ishiwata M.D., Ph.D. ,&nbsp;Sruthi Tatavarthi B.S. ,&nbsp;Jasmine Diallo B.A. ,&nbsp;Rachel Michel M.S.P.H. ,&nbsp;M. Soriful Islam Ph.D. ,&nbsp;Mostafa A. Borahay M.D., Ph.D.","doi":"10.1016/j.xfss.2025.12.005","DOIUrl":"10.1016/j.xfss.2025.12.005","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the effect of angiotensin II (Ang II) on proliferation and extracellular matrix (ECM) deposition in human uterine leiomyoma cells and normal myometrial cells.</div></div><div><h3>Design</h3><div>Experimental in vitro study using immortalized human leiomyoma (HuLM) cells, immortalized human uterine smooth muscle (UTSM) cells, and patient-derived primary fibroid and myometrial cells.</div></div><div><h3>Subjects</h3><div>Women with uterine fibroids who underwent hysterectomy.</div></div><div><h3>Exposure</h3><div>Administration of physiological and supraphysiological levels of Ang II (to mimic essential hypertension) to cultured HuLM, UTSM, primary fibroid, and myometrial cells to assess effects on cellular proliferation and ECM deposition.</div></div><div><h3>Main Outcome Measures</h3><div>We evaluated HuLM, UTSM, primary fibroid, and UTSM cells for the presence of the Ang II type 1 receptor. Angiotensin II-induced proliferation and ECM deposition was assessed through MTS assay, Western blot analysis, immunofluorescence, and real-time polymerase chain reaction.</div></div><div><h3>Results</h3><div>Immortalized and primary leiomyoma and myometrial cells expressed Ang II type 1 receptor. Leiomyoma cells responded to Ang II with increased cellular proliferation measured by MTS assay, proliferating cell nuclear antigen protein levels, and Ki67 staining. The Ang II treatment of fibroid cells showed increased expression of Collagen 1A1, the predominant fibroid and myometrial collagen. Integrin β1, an upstream regulator of fibrosis, also showed an increase in protein and messenger ribonucleic acid expression in fibroid cells treated with Ang II. No difference in proliferation or ECM production was observed in myometrial cell controls.</div></div><div><h3>Conclusion</h3><div>Angiotensin II promoted growth and matrix accumulation in fibroid cells, highlighting a potential link between fibroids and cardiovascular disease.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"7 2","pages":"Pages 172-183"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145758552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiles of human parthenogenotes: contribution of the maternal genome across the early embryo development","authors":"Xavier Vendrell Ph.D. ,&nbsp;Pedro De Castro M.S. ,&nbsp;Laura Escrich Ph.D. ,&nbsp;Noelia Grau Ph.D. ,&nbsp;Roberto González-Martín Ph.D. ,&nbsp;Alicia Quiñonero M.S. ,&nbsp;Arancha Galán Ph.D. ,&nbsp;Francisco Domínguez Ph.D. ,&nbsp;María-José Escribà Ph.D.","doi":"10.1016/j.xfss.2025.12.003","DOIUrl":"10.1016/j.xfss.2025.12.003","url":null,"abstract":"<div><h3>Objective</h3><div>To study the maternal contribution to early human embryogenesis by describing the transcriptional dynamics and regulatory roles of maternal effect genes (MEGs) and transcription factors (TFs) throughout the first four cell cycles. This will be achieved using parthenogenotes, such as the human uniparental bioconstruct model.</div></div><div><h3>Design</h3><div>Descriptive observational study based on single-cell transcriptomic analysis.</div></div><div><h3>Subjects</h3><div>A total of 19 single human parthenocytes were derived from six parthenogenotes at the first (n = 2), third (n = 2), and fourth (n = 2) cell cycles.</div></div><div><h3>Exposure</h3><div>Transcriptomic changes occurring during early embryonic development in the absence of paternal genomic input.</div></div><div><h3>Main Outcome Measures</h3><div>Transcript abundance of MEGs, expression levels of key TFs, number and identity of differentially expressed genes (DEGs), pathway enrichment associated with DEGs, transcriptional complexity, temporal patterns of MEG transcript decay and persistence and timing of embryonic genome activation (EGA).</div></div><div><h3>Results</h3><div>Transcriptomic analysis revealed progressive increases in transcriptional complexity, with major shifts between the third and fourth cell cycles coinciding with EGA. A total of 212 and 1,515 DEGs were identified in the third and fourth cycles, respectively (fold change ≥|2| vs. first cycle), predominantly involved in ribonucleic acid biosynthesis and cell proliferation pathways. Principal component and hierarchical clustering analyses showed distinct transcriptomic profiles by cell cycle and oocyte origin. Key TFs (<em>DUXA, DUX4, Elk-1, E2F-1, Sp1</em>) were implicated in cell cycle regulation. The MEG analysis revealed decay of transcripts associated with messenger ribonucleic acid clearance, alongside sustained expression of MEGs linked to cell cycle progression and spindle assembly, suggesting a nonrandom, structured maternal regulatory program.</div></div><div><h3>Conclusion</h3><div>This study provides the first comprehensive single-cell transcriptomic characterization of early human parthenogenotes, suggesting a structured, genome-driven maternal program that governs early embryonic development in the absence of paternal input. The identification of key TFs and MEG signature highlights the pivotal regulatory role of the maternal genome before EGA and may inform strategies to improve outcomes in assisted reproductive technologies.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"7 2","pages":"Pages 150-162"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145727605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phase-dependent cytokine dynamics in multicellular in vitro models of the human endometrium","authors":"Mark Gavriel B.Sc., M.B.A., M.D. ,&nbsp;Ariel J. Jaffa M.D. ,&nbsp;David Elad D.Sc. ,&nbsp;Dan Grisaru M.D., Ph.D.","doi":"10.1016/j.xfss.2025.12.001","DOIUrl":"10.1016/j.xfss.2025.12.001","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;div&gt;To characterize the dynamic secretion patterns of extracellular cytokines in 3 in vitro human endometrial culture models subjected to a hormonally simulated 28-day menstrual cycle and to assess the influence of cellular composition on paracrine signaling relevant to endometrial receptivity.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Design&lt;/h3&gt;&lt;div&gt;Experimental in vitro study.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Subjects&lt;/h3&gt;&lt;div&gt;Three endometrial culture models were developed using combinations of endometrial epithelial cells (RL95-2), stromal cells (T0533), and primary myometrial smooth muscle cells.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Exposures&lt;/h3&gt;&lt;div&gt;The 3 multicellular models were subjected to a sequential hormonal treatment protocol simulating the proliferative, ovulatory, and secretory phases of the human menstrual cycle.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Main Outcome Measures&lt;/h3&gt;&lt;div&gt;Quantitative profiling of 105 extracellular cytokines across 4 hormonal phases (control, proliferative, ovulatory, and secretory) using a cytokine array platform. We conducted a comparative analysis of cytokine expression patterns, correlation matrices, and hierarchical clustering to identify model-specific and hormone-dependent regulatory networks.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;We identified 12 highly expressed cytokines with distinct phase and model-specific expression profiles. Extracellular matrix metalloproteinase inducer, macrophage migration inhibitory factor, and interleukin 8 levels increased consistently across all phases, peaking during the window of implantation. Vascular endothelial growth factor exhibited biphasic expression patterns; epidermal growth factor level was up-regulated by estradiol and down-regulated by progesterone. Dickkopf-related protein 1 was up-regulated in progesterone-dominant phases. Serpin E1 and Dickkopf-related protein 1 showed phase-specific regulation and were also influenced by cellular composition. Insulin-like growth factor-binding protein 3 down-regulated the proliferative and ovulatory phases. Clustering analyses revealed coregulatory modules (e.g., extracellular matrix metalloproteinase inducer/macrophage migration inhibitory factor and growth-regulated oncogen-α /lipocalin-2) and distinct cytokine networks modulated by stromal and myometrial components. Notably, model C (endometrial epithelial cells [EEC] + endometrial stromal cells + myometrial smooth muscle cells) demonstrated profiles more similar to model A (EEC alone) than to model B (EEC + endometrial stromal cells), suggesting myometrial smooth muscle cells may attenuate certain epithelial-stromal paracrine interactions.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;The phase-specific and model-dependent cytokine secretion patterns highlight the complexity of endometrial paracrine signaling and underscore the importance of multicellular in vitro models. These findings advance our understanding of cytokine dynamics during the menstrual cycle and provide a platform for future studies on endome","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"7 2","pages":"Pages 138-149"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145710183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial oocytes through haploidization of mouse endometrial stromal cells and bone marrow-derived mesenchymal stem cells","authors":"Shelun Tsai M.D. ,&nbsp;Philip Xie B.S. ,&nbsp;Stephanie Cheung M.Sc. ,&nbsp;Zev Rosenwaks M.D. ,&nbsp;Gianpiero D. Palermo M.D., Ph.D.","doi":"10.1016/j.xfss.2025.12.002","DOIUrl":"10.1016/j.xfss.2025.12.002","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the feasibility of using endometrial stromal cells (EmSCs) and bone marrow-derived mesenchymal stem cells (BM-MSCs) as alternative sources of donor nuclei for somatic cell haploidization.</div></div><div><h3>Design</h3><div>To perform somatic cell haploidization, mouse metaphase II oocytes were enucleated and injected with the nucleus of cumulus cells (CCs), EmSCs or BM-MSCs. Intact metaphase II oocytes served as controls. Oocytes after haploidization and controls were inseminated and cultured up to 96 hours in a time-lapse incubator to assess embryo development and morphokinesis. Blastocysts were either cryopreserved for future genetic analysis or transferred to surrogate pseudo-pregnant mice.</div></div><div><h3>Subjects</h3><div>Female B2D2F1 mice (ooplasm donor), male B6-EGFP mice (spermatozoa donor), CD-1 female mice (surrogate)</div></div><div><h3>Exposure</h3><div>Enucleated oocytes underwent somatic cell nuclear transfer using the nucleus of CCs, EmSCs or BM-MSCs to generate functional oocytes. These oocytes are fertilized to generate conceptuses.</div></div><div><h3>Main Outcome Measures</h3><div>The primary outcome compared embryo development in the experimental groups vs. control. The secondary outcome was embryo morphokinetics evaluated with time-lapse microscopy.</div></div><div><h3>Results</h3><div>A total of 811 oocytes were enucleated, with a survival rate of 97.5%. Somatic cell nuclear transfer (SCNT) was performed using CCs (n = 90), EmSCs (n = 394), or BM-MSCs (n = 327), resulting in comparable somatic cell fusion rates of 95.2%, 96.0%, and 96.5%, respectively. Comparing the different SCNT groups, the CC cohort had a higher fertilization rate (45.6%) than the EmSC (30.8%) and BM-MSC (26.5%) cohorts. However, subsequent embryo development showed significant attrition in the CC cohort, where only 14.4% of CC embryos developed into blastocysts compared with 25.6% of EmSC embryos and 19.6% of BM-MSC embryos. In terms of embryo morphokinetics, all SCNT groups had slower embryo progression than the control group. However, EmSC and BM-MSC embryo development was faster than CC embryos from syngamy to the 8-cell stage (48.8 vs. 48.5 vs. 58.5 hours, respectively). All EmSC embryos and eight out of nine BM-MSC embryos displayed heterozygosity, confirming biparental contribution from the somatic cell and sperm genome. In the EmSC cohort, 42 blastocysts were transferred, yielding three healthy mouse pups (2 male, 1 female). All three pups displayed biparental inheritance, grew to adulthood and produced three healthy first-generation litters.</div></div><div><h3>Conclusion</h3><div>Somatic cell haploidization of EmSCs and BM-MSCs generated oocytes capable of full preimplantation development and yielded healthy offspring. The utilization of genotyped donor nuclei for neogametogenesis may represent a feasible fertility treatment option for individuals with complete absence of oocytes.</div></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"7 2","pages":"Pages 113-123"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145727641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of triclosan on ovarian reserve and fecundity in a mouse model.","authors":"Katherine M Baker, Julia N McAdams, Morgan F Woodman-Sousa, Payton De La Cruz, Kathryn J Grive","doi":"10.1016/j.xfss.2026.02.001","DOIUrl":"10.1016/j.xfss.2026.02.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the acute and chronic effects of triclosan (TCS) exposure on ovarian reserve dynamics and reproductive potential in a mouse model.</p><p><strong>Design: </strong>Female C57BL/6 mice were given a diet containing TCS 10 mg/kg per day or a control diet starting on postnatal day (PND) 30. Ovaries were collected at PND 37, 60, 90, 210, 300, and 360, reflecting 1 week, 1 month, 2 month, 6 month, 9 month, and 12 month periods of exposure. Ovarian follicular compositions were analyzed in both groups at all exposure time points. Animals from both groups at the same exposure time groups were bred with age-matched control C57BL/6 male mice and the number of litters, pups per litter, interlitter interval, and number of deceased pups were recorded.</p><p><strong>Subjects: </strong>C57BL/6 mice.</p><p><strong>Exposure: </strong>Triclosan 10 mg/kg per day diet or control diet.</p><p><strong>Main outcome measures: </strong>Ovarian follicular compositions were analyzed in TCS-treated mice compared with controls at both acute and chronic exposure time points. Animals from both exposure groups were bred and the number of litters, pups per litter, interlitter time interval, and number of deceased pups were analyzed.</p><p><strong>Results: </strong>Primordial follicle density decreases with age in control and TCS-exposed mice with a trend toward an accelerated decline in the PND 90, 300, and 360 TCS-exposed groups. The TCS-exposed mice at PND 37 and PND 60 both exhibit a trend toward decreased growing follicle (primary and secondary) densities. At PND 300 and 360, there are decreased follicular densities across all follicle types in the TCS-exposed compared with control groups with lower antral follicle densities (0.27 vs. 0.04 at PND 300 and 0.22 vs. 0.06 at PND 360). Litter sizes were similar between groups across all time points. The length of time between first and fifth litters is increased in the TCS-exposed group at PND 37 (116 days vs. 135.2 days), with similar trends seen in the PND 60 (114.7 days vs. 122.8 days) and PND 90 (140.8 vs. 158.8 days) groups, which may suggest longer cycles and reduced rates of ovulation in midreproductive life. No increase in stillbirth was seen in the TCS-exposure groups compared with controls.</p><p><strong>Conclusions: </strong>Our study demonstrates that TCS exposure may accelerate normal age-related ovarian follicular loss with decreased antral follicle density at chronic exposure timepoints. Triclosan exposure may also impact estrous cycles at midreproductive age.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146222379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The metabolic landscape of the maternal-fetal interface in missed miscarriage: a cross-sectional pilot multiomics study.","authors":"Haoyue Hu, Qiqi Liu, Yingyu Liu, Jiao Cheng, Shuwei Zheng, Jing Ruan, Simin Liu, Yanfang Wang, Yuanfang Zhu, Xiaoyan Chen","doi":"10.1016/j.xfss.2026.01.001","DOIUrl":"10.1016/j.xfss.2026.01.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the metabolic alterations at the maternal-fetal interface in missed miscarriage (MM) using untargeted metabolomic and lipidomic profiling of paired villous and decidual tissues.</p><p><strong>Design: </strong>Observational study utilizing multiomics integration to analyze metabolic and lipidomic profiles.</p><p><strong>Subjects: </strong>A total of 10 women were recruited in this study, including 5 women with MM and 5 healthy controls. All cases included in the MM group were euploid, excluding chromosomal abnormalities.</p><p><strong>Exposure: </strong>The exposure in this study was the condition of MM, with tissue samples collected from both villous and decidual tissues.</p><p><strong>Main outcome measures: </strong>Differentially abundant metabolites and lipids between MM and control groups, focusing on metabolic pathways related to glycerophospholipid metabolism, sphingolipid signaling, and amino acid metabolism.</p><p><strong>Results: </strong>We identified significant metabolic alterations in both villous and decidual tissues from MM pregnancies compared with healthy controls. Key findings included the downregulation of amino acids and organic acids, such as lactic acid, suggesting impaired energy metabolism. Lipidomic analysis revealed alterations in glycerophospholipids and sphingolipids, indicating disrupted cell signaling and inflammatory pathways in MM.</p><p><strong>Conclusion: </strong>This multiomics study highlights specific metabolic and lipidomic disruptions in MM, suggesting early metabolic disturbances at the maternal-fetal interface correlate with miscarriage. These findings may guide future therapeutic strategies targeting metabolic pathways to improve pregnancy outcomes in MM.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146041959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}