Extracellular vesicles and circulating nucleic acids最新文献_第6页

Leveraging biomimetic synthesis strategy for next-generation dendritic cell nanovaccines 利用仿生合成策略制备下一代树突状细胞纳米疫苗

Extracellular vesicles and circulating nucleic acids Pub Date : 2022-01-01 DOI: 10.20517/evcna.2022.35

Yutian Xia, Jianzhong Zhang

引用次数: 0

Towards extracellular vesicle delivery systems for tissue regeneration: material design at the molecular level 组织再生的细胞外囊泡输送系统:分子水平上的材料设计

Extracellular vesicles and circulating nucleic acids Pub Date : 2022-01-01 DOI: 10.20517/evcna.2022.37

A. Chen, Heng-Song Tian, Nana Yang, Zhi-jun Zhang, Guo-Yuan Yang, W. Cui, Yaohui Tang

{"title":"Towards extracellular vesicle delivery systems for tissue regeneration: material design at the molecular level","authors":"A. Chen, Heng-Song Tian, Nana Yang, Zhi-jun Zhang, Guo-Yuan Yang, W. Cui, Yaohui Tang","doi":"10.20517/evcna.2022.37","DOIUrl":"https://doi.org/10.20517/evcna.2022.37","url":null,"abstract":"The discovery and development of extracellular vesicles in tissue engineering have shown great potential for tissue regenerative therapies. However, their vesicle nature requires dosage-dependent administration and efficient interactions with recipient cells. Researchers have resorted to biomaterials for localized and sustained delivery of extracellular vesicles to the targeted cells, but not much emphasis has been paid on the design of the materials, which deeply impacts their molecular interactions with the loaded extracellular vesicles and subsequent delivery. Therefore, we present in this review a comprehensive survey of extracellular vesicle delivery systems from the viewpoint of material design at the molecular level. We start with general requirements of the materials and delve into different properties of delivery systems as a result of different designs, from material selections to processing strategies. Based on these differences, we analyzed the performance of extracellular vesicle delivery and tissue regeneration in representative studies. In light of the current missing links within the relationship of material structures, physicochemical properties and delivery performances, we provide perspectives on the interactions of materials and extracellular vesicles and the possible extension of materials. This review aims to be a strategic enlightenment for the future design of extracellular vesicle delivery systems to facilitate their translation from basic science to clinical applications.","PeriodicalId":73008,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81245111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Exploring extracellular vesicles as mediators of clinical disease and vehicles for viral therapeutics: Insights from the COVID-19 pandemic. 探索细胞外囊泡作为临床疾病的介质和病毒治疗的载体:来自COVID-19大流行的见解。

Extracellular vesicles and circulating nucleic acids Pub Date : 2022-01-01 Epub Date: 2022-07-19 DOI: 10.20517/evcna.2022.19

Vaughn D Craddock, Christine M Cook, Navneet K Dhillon

引用次数: 1

Extracellular vesicles in reproduction and pregnancy 生殖和妊娠中的细胞外囊泡

Extracellular vesicles and circulating nucleic acids Pub Date : 2022-01-01 DOI: 10.20517/evcna.2022.27

Tahlia I. Smith, A. Russell

{"title":"Extracellular vesicles in reproduction and pregnancy","authors":"Tahlia I. Smith, A. Russell","doi":"10.20517/evcna.2022.27","DOIUrl":"https://doi.org/10.20517/evcna.2022.27","url":null,"abstract":"Extracellular vesicles (EVs) are small, lipid-bound packages that are secreted by all cell types and have been implicated in many diseases, such as cancer and neurodegenerative disorders. Though limited, an exciting new area of EV research focuses on their role in the reproductive system and pregnancy. In males, EVs have been implicated in sperm production and maturation. In females, EVs play a vital role in maintaining reproductive organ homeostasis and pregnancy, including the regulation of folliculogenesis, ovulation, and embryo implantation. During the development and maintenance of a pregnancy, the placenta is the main form of communication between the mother and the developing fetus. To support the developing fetus, the placenta will act as numerous vital organs until birth, and release EVs into the maternal and fetal bloodstream. EVs play an important role in cell-to-cell communication and may mediate the pathophysiology of pregnancy-related disorders such as preeclampsia, gestational diabetes mellitus, preterm birth, and intrauterine growth restriction, and potentially serve as noninvasive biomarkers for these conditions. In addition, EVs may also mediate processes involved in both male and female infertility. Together, the EVs secreted by both the male and female reproductive tracts work to promote reproductive fertility and play vital roles in mediating maternal-fetal crosstalk and pregnancy maintenance.","PeriodicalId":73008,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89789591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A kaleidoscopic view of extracellular vesicles in lysosomal storage disorders 溶酶体贮积症中细胞外囊泡的万花筒观察

Extracellular vesicles and circulating nucleic acids Pub Date : 2022-01-01 DOI: 10.20517/evcna.2022.41

Charlotte V Hegeman, Olivier G. de Jong, M. Lorenowicz

{"title":"A kaleidoscopic view of extracellular vesicles in lysosomal storage disorders","authors":"Charlotte V Hegeman, Olivier G. de Jong, M. Lorenowicz","doi":"10.20517/evcna.2022.41","DOIUrl":"https://doi.org/10.20517/evcna.2022.41","url":null,"abstract":"Extracellular vesicles (EVs) are a heterogeneous population of stable lipid membrane particles that play a critical role in the regulation of numerous physiological and pathological processes. EV cargo, which includes lipids, proteins, and RNAs including miRNAs, is affected by the metabolic status of the parental cell. Concordantly, abnormalities in the autophagic-endolysosomal pathway, as seen in lysosomal storage disorders (LSDs), can affect EV release as well as EV cargo. LSDs are a group of over 70 inheritable diseases, characterized by lysosomal dysfunction and gradual accumulation of undigested molecules. LSDs are caused by single gene mutations that lead to a deficiency of a lysosomal protein or lipid. Lysosomal dysfunction sets off a cascade of alterations in the endolysosomal pathway that can affect autophagy and alter calcium homeostasis, leading to energy imbalance, oxidative stress, and apoptosis. The pathophysiology of these diseases is very heterogenous, complex, and currently incompletely understood. LSDs lead to progressive multisystemic symptoms that often include neurological deficits. In this review, a kaleidoscopic overview will be given on the roles of EVs in LSDs, from their contribution to pathology and diagnostics to their role as drug delivery vehicles. Furthermore, EV cargo and surface engineering strategies will be discussed to show the potential of EVs in future LSD treatment, both in the context of enzyme replacement therapy, as well as future gene editing strategies like CRISPR/Cas. The use of engineered EVs as drug delivery vehicles may mask therapeutic cargo from the immune system and protect it from degradation, improving circulation time and targeted delivery.","PeriodicalId":73008,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75121118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liquid biopsy of extracellular vesicle biomarkers for prostate cancer personalized treatment decision 细胞外囊泡液体活检生物标志物对前列腺癌个性化治疗决策的影响

Extracellular vesicles and circulating nucleic acids Pub Date : 2022-01-01 DOI: 10.20517/evcna.2021.20

Meng Han, Bairen Pang, Cheng Zhou, Xin Li, Qi Wang, Junhui Jiang, Yong Li

引用次数: 5

Acknowledgement to reviewers of Extracellular Vesicles and Circulating Nucleic Acids in 2021 感谢2021年《细胞外囊泡和循环核酸》的审稿人

Extracellular vesicles and circulating nucleic acids Pub Date : 2022-01-01 DOI: 10.20517/evcna.2022.01

引用次数: 0

Liquid biopsy for monitoring medulloblastoma 液体活检监测成神经管细胞瘤

Extracellular vesicles and circulating nucleic acids Pub Date : 2022-01-01 DOI: 10.20517/evcna.2022.36

R. Eibl, M. Schneemann

{"title":"Liquid biopsy for monitoring medulloblastoma","authors":"R. Eibl, M. Schneemann","doi":"10.20517/evcna.2022.36","DOIUrl":"https://doi.org/10.20517/evcna.2022.36","url":null,"abstract":"Despite recent progress in molecular diagnostics defining four distinct medulloblastoma groups, the clinical management of these malignant childhood tumors of the cerebellum remains challenging. After surgical removal of the tumor, both cytotoxic chemotherapy and irradiation can offer additional curative benefits, but they also include a significant risk of long-term damage. Early molecular profiling aims to predict the outcome of such aggressive therapies. This prevents unnecessary damage to patients who may not need it and helps to identify those patients with remaining tumor cells who may benefit from more aggressive treatment with the intent to cure. Monitoring tumor evolution in real time allows personalized precision medicine with an immediate clinical response resulting in a better outcome. Liquid biopsy includes various methodologies already applied in numerous studies and clinical trials for common cancers including brain tumors, but information on medulloblastomas is limited. This review summarizes the recent developments of how liquid biopsy can support or even replace the standard monitoring of medulloblastomas by medical imaging or cytology and discusses what will be needed to make liquid biopsy a new gold standard in diagnosis, therapy, and follow-up of medulloblastomas for the benefit of the patients.","PeriodicalId":73008,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75645476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Emerging frontiers of cell-free DNA fragmentomics 无细胞DNA片段组学的新兴前沿

Extracellular vesicles and circulating nucleic acids Pub Date : 2022-01-01 DOI: 10.20517/evcna.2022.34

Xi Hu, Spencer C. Ding, P. Jiang

引用次数: 0

Genome-wide Analysis Reflects Novel 5-Hydroxymethylcytosines Implicated in Diabetic Nephropathy and the Biomarker Potential. 全基因组分析反映了与糖尿病肾病有关的新型5-羟甲基胞嘧啶和生物标志物潜力。

Extracellular vesicles and circulating nucleic acids Pub Date : 2022-01-01 DOI: 10.20517/evcna.2022.03

Ying Yang, C. Zeng, Kun Yang, Shaohua Xu, Zhou Zhang, Qinyun Cai, Chuan He, Wei Zhang, Song-Mei Liu

{"title":"Genome-wide Analysis Reflects Novel 5-Hydroxymethylcytosines Implicated in Diabetic Nephropathy and the Biomarker Potential.","authors":"Ying Yang, C. Zeng, Kun Yang, Shaohua Xu, Zhou Zhang, Qinyun Cai, Chuan He, Wei Zhang, Song-Mei Liu","doi":"10.20517/evcna.2022.03","DOIUrl":"https://doi.org/10.20517/evcna.2022.03","url":null,"abstract":"Aim Diabetic nephropathy (DN) has become the most common cause of end-stage renal disease (ESRD) in most countries. Elucidating novel epigenetic contributors to DN can not only enhance our understanding of this complex disorder, but also lay the foundation for developing more effective monitoring tools and preventive interventions in the future, thus contributing to our ultimate goal of improving patient care. Methods The 5hmC-Seal, a highly selective, chemical labeling technique, was used to profile genome-wide 5-hydroxymethylcytosines (5hmC), a stable cytosine modification type marking gene activation, in circulating cell-free DNA (cfDNA) samples from a cohort of patients recruited at Zhongnan Hospital, including T2D patients with nephropathy (DN, n = 12), T2D patients with non-DN vascular complications (non-DN, n = 29), and T2D patients without any complication (controls, n = 14). Differentially analysis was performed to find DN-associated 5hmC features, followed by the exploration of biomarker potential of 5hmC in cfDNA for DN using a machine learning approach. Results Genome-wide analyses of 5hmC in cfDNA detected 427 and 336 differential 5hmC modifications associated with DN, compared with non-DN individuals and controls, and suggested relevant pathways such as NOD-like receptor signaling pathway and tyrosine metabolism. Our exploration using a machine learning approach revealed an exploratory model comprised of ten 5hmC genes showing the possibility to distinguish DN from non-DN individuals or controls. Conclusion Genome-wide analysis suggests the possibility of exploiting novel 5hmC in patient-derived cfDNA as a non-invasive tool for monitoring DN in high risk T2D patients in the future.","PeriodicalId":73008,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74925619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3