European journal of biomedical research最新文献

{"title":"A Synchronous Blind Test and Methodological Comparison Between Colloidal Gold Rapid Test vs RT-PCR For Sars-Cov-2 Detection","authors":"A. Mourtzikou, D. Tsiftsis, Maria Agrogianni, Nikolaos Kasimatis, Anthoula Dritsa, M. Stamouli, Panagiotis Koumpouros, Christina Seitopoulou, Elpida Toka","doi":"10.24018/ejbiomed.2022.1.5.26","DOIUrl":"https://doi.org/10.24018/ejbiomed.2022.1.5.26","url":null,"abstract":"Background: The current pandemic due to novel SARS-CoV-2 virus dramatically affected health care systems and public health worldwide. The present study aimed to evaluate two analytical methods, colloidal gold antigen rapid test vs reference PCR for the detection of SARS-CoV-2. The patients enrolled in the trial were admitted at the emergency department of a tertiary care hospital, with symptoms of suspected COVID-19 disease.\u0000Methods: A total of 300 patients participated in the study. Patients’ age, gender and result from the Colloidal antigen rapid test were recorded. PCR detection for SARS-CoV-2 was then applied, according to the manufacturer’s instructions. Statistical analysis of all collected data was performed for sensitivity, specificity, ROC curves, PV+, PV- and Cohen’s kappa coefficient. McNemar’s chi-squared test and p-values were also tested.\u0000Results: A p-value=0.045 from McNemar’s chi-squared test for CI 95% was observed, so H0 marginally is not rejected. The sensitivity of colloidal gold antigen rapid test was 79%, the specificity 96%, PV+89%, PV-91% and the kappa coefficient=0.79 (>0.5) that correlates to substantial agreement according to Cohen’s Kappa interpretation.\u0000Conclusions: Through the methodological comparisons and according to WHO guidelines for the sensitivity, specificity and kappa coefficient that correlates to substantial agreement the Colloidal Gold Antigen Rapid Test for SARS-CoV-2 meet the needs of clinical test in the emergency unit playing an important role in the context of mass patient screening and screening in remote areas.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84800849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Ethanol Extract of Tangse Liberica Coffee (Coffea liberica) Inhibited the Reduction of Leydig Cell Number and Testosterone Levels in Male Wistar Rats (Rattus norvegicus) Exposed to Ultraviolet B Light","authors":"Dhea Frisilla, W. Pangkahila, A. E. Darwinata","doi":"10.24018/ejbiomed.2022.1.5.24","DOIUrl":"https://doi.org/10.24018/ejbiomed.2022.1.5.24","url":null,"abstract":"Background: Aging is a complex mechanism related to various factors including free radicals. Free radicals that occur due to exposure to ultraviolet light can cause oxidative stress and damage the spermatogenesis process, including Leydig cells and testosterone. Therefore, antioxidants play a very important role in inhibiting these reactions, which are abundant in coffee. This study aimed to analyze the effect of the ethanol extract of Tangse liberica coffee (Coffea liberica) in inhibiting the decrease in Leydig cell number and testosterone levels in male Wistar (Rattus norvegicus) rats exposed to Ultraviolet B light.\u0000Methods: This study includes a post-test-only control group type of laboratory experimental study. The research group was divided into three groups, namely positive control (without giving coffee), treatment 1 (giving coffee extract 216 mg/kgBW), and treatment 2 (giving coffee extract 432 mg/kg BW). A total of 30 rats were used in this study and exposed to UV-B 0.225 MW/cm² 8 hours per day for 28 days. Testosterone assessment was carried out by examining blood specimens and Leydig cell counts were carried out histologically with Hematoxylin-Eosin staining. One-way ANOVA analysis and Kruskal Wallis test followed by post hoc test were performed in this study with a significance level of 95%.\u0000Results: The result showed a significant difference in the number of Leydig cells between the study groups (p = 0.016) with the highest mean in group 1, 48.27 9.12 cells/LFoV. The same thing also happened to the difference in testosterone levels between groups (p < 0.001) with the most dominant mean in treatment group 2 being 76.42 nmol/L.\u0000Conclusion: The conclusion of this research is the administration of the ethanol extract of Tangse liberica coffee (Coffea liberica) can inhibit the decrease in Leydig cells number and testosterone levels in male Wistar (Rattus norvegicus) rats exposed to Ultraviolet B light.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80162798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of HER2/neu among Female with Invasive Ductal Breast Carcinoma, Using Manual Tissue Microarray Technology in Khartoum State, Sudan","authors":"R. Sidahmed, A. Babiker, E. A. Ahmed, Alaa E. Abbas","doi":"10.24018/ejbiomed.2022.1.4.20","DOIUrl":"https://doi.org/10.24018/ejbiomed.2022.1.4.20","url":null,"abstract":"The human epidermal growth factor receptor-2 (HER2), which is expressed in large amount in 20%-30% of breast cancer patients, is associated with disease recurrences, shortened disease survival, and bad prognosis. The aim of this study is to evaluate the expression of (Her2/neu) receptor as a diagnostic and prognostic marker in Invasive Ductal Breast cancer in Sudanese female patients. The study was performed on 35 archival paraffin blocks previously diagnosed as invasive ductal breast cancer (IDC), then a second diagnosis was done using IHC staining (Her2/neu). The obtained information is arranged in a standard master sheet, then analyzed using a statistical package for social science (SPSS) version 20 computer program. The age 30-45years was the most frequent age group 48.6% (17/35), followed by 46-60 years 40% (14/35). The majority of samples were grade III 74.3% (26/35) and the samples of grade II were 25.7% (9/35). the majority of TMA results were positive for Her2/neu receptor 65.7% (23/35) and the negative results were 34.3% (12/35) the majority of TMA score were (+) 31.4% (11/35), followed by (++) 17.2% (6/35), then (+++) 11.4% (4/35), then (++++) 5.7% (2/35), and the negative result were, 34.3% (12/35). There was a significant relation between H&E diagnosis with TMA result for Her2/ neu diagnosis that the P-value (0.0002). There was an insignificance relation between H&E diagnosis of grades with TMA results for Her2/ neu diagnosis that the P-value (0.08). In conclusion, HER2 is over-expressed in 65.7% of the patient while internationally it is over-expressed in 15-30%. This provides valuable prognostic and predictive implications in order to choose the suitable treatment for the patients.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74845648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Assessment of Knowledge, Practice and Barriers of Voluntary Blood Donation among Staff and Patients of a Tertiary Hospital in Nigeria","authors":"Aondona David Daniel, N. Akwaras, Matthew Ngbede Ocheifa, Chukwuemeka Nwaeze, Laadi T. Swende, Bamidele Ohiozoje Ornguga, Rufus IfechukwuIzeji","doi":"10.24018/ejbiomed.2022.1.4.17","DOIUrl":"https://doi.org/10.24018/ejbiomed.2022.1.4.17","url":null,"abstract":"\u0000\u0000\u0000\u0000Background: Blood and blood products are essential resources in the management of many health conditions. It has been tasking to achieve complete reliance on voluntary unpaid blood donors. Hence, assessing the knowledge, practices and barriers of voluntary blood donation can impact on improvement of the number of voluntary blood donors.\u0000Aim: To assess the knowledge, practice, and barriers of voluntary blood donation among the participants.\u0000Method: A cross sectional descriptive study was carried out using pre-tested interviewer administered questionnaire administered to 288 participants selected by systematic random sampling technique. The data was analysed using SPSS version 20.\u0000\u0000\u0000\u0000\u0000Results: The mean age of the respondents was 38.81 ± 11.67. Males were slightly more (51.70%) than females (n=149, 51.70%). Most were married (n=204, 70.80%), of the Tiv tribe (n=160, 55.60%) and were Christians (n=263, 91.30). Over half had tertiary education (n=113, 55.20%), were civil servants (n=159, 55.20%) and worked in non-health related disciplines (n=168, 58.30%) respectively. Over two-third reside in rural areas (n=206, 71.50%). 0nly 30.56% had good knowledge scores. Those who had ever voluntarily donated blood made up 11.1%. Females were less likely to donate blood (aOR=0.19, CI 0.08 – 0.48, p=0.00). Those with tertiary education were nearly six times more likely to have donated blood (aOR=5.92, CI 1.66 – 21.10, p=0.01). Those with non-health related jobs (aOR=0.00, CI 0.06 – 0.46, p=0.00) were less likely to donate blood. The most common reason for deferral was viral infections (37.50%) such as hepatitis B, hepatitis C and HIV infection. The commonest barriers of voluntary blood donation fear of blood being sold for rituals (22.6%), fear (22.9) fear of needle prick (16.7%).","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77151174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling Approaches for Fluidic Mass Transport in Next Generation Micro and Nano Biomedical Sensors","authors":"Siyua Cao, Naveen Weerasekera, Dawa Ram Shingdan","doi":"10.24018/ejbiomed.2022.1.3.12","DOIUrl":"https://doi.org/10.24018/ejbiomed.2022.1.3.12","url":null,"abstract":"This review discusses on current methodologies and trends in modeling fluidic mass transport phenomena in micro and nano scale biomedical devices. We have presented the governing equations for species transport in micro and nano scales and provided analytical as well as computational approaches that can aid in obtaining solutions for complex flow problems. We have also reviewed novel methodologies that modern research community utilized for simulating species transport in micro and nano biomedical sensing devices.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86168161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suspected COVID-19 Cases Admitted in a Tertiary Care Hospital. Correlation of Demographic and Clinical Characteristics with Viral Load Results and Hospitalization","authors":"A. Mourtzikou, Antonia K Korre, M. Stamouli, Christina Seitopoulou, Ioanna Petraki, Georgia Kalliora, Panagiotis Koumpouros, Paraskevi Karle, Maria Kimouli","doi":"10.24018/ejbiomed.2022.1.2.6","DOIUrl":"https://doi.org/10.24018/ejbiomed.2022.1.2.6","url":null,"abstract":"Background: The current pandemic outbreak of COVID-19 due to the SARS-CoV-2 virus affected the health care systems, health services and economy globally. It also affected the health of the population worldwide, with high mortality and morbidity rates. The present study aimed to study the patients that were admitted to a tertiary care hospital and to investigate the potential correlation between hospitalization and RT-PCR for SARS-CoV-2 results with demographic characteristics and clinical characteristics. Moreover, it aimed to examine a mathematical formula that might describe the correlation of the aforementioned parameters. \u0000Methods: The study population included 1244 patients admitted to the Nikea General Hospital \"Agios Panteleimon\", Piraeus, Greece. Patient age, gender, underlying diseases, travel history, symptoms, etiology for hospital admission and contact with confirmed cases were recorded. Potential correlation of hospitalization and RT-PCR for SARS-CoV-2 results with the aforementioned characteristics were identified by chi-square test of independence and logistic regression analysis. \u0000Results: We observed significant correlation of hospitalization with fever, cough, dyspnea, pneumonia, travel history and etiology for hospital admission. We observed significant correlation of RT-PCR for SARS-CoV-2 results with rapid antigen test result, hospitalization etiology for hospital admission and contact with confirmed COVI-19 case. \u0000Conclusions: According to the logistic regression model, RT-PCR for SARS-CoV-2 result, fever, dyspnea, pneumonia, and underlying disease are the most important predictors for hospitalization in the population under study. Contact with confirmed COVID-19 case is the most important predictor for RT-PCR for SARS-CoV-2 result.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89927512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Biochemical Test Results in Patients with COVID-19 Infection","authors":"M. Stamouli, Sofia Kougioumtzidou, A. Mourtzikou, Antonia K Korre, Georgia Kalliora, Panagiotis Koumpouros, Maria Tsesmeli, Vasiliki Mpourtsala, Anastasios Skliris, C. Stergiou","doi":"10.24018/ejbiomed.2022.1.1.5","DOIUrl":"https://doi.org/10.24018/ejbiomed.2022.1.1.5","url":null,"abstract":"Background: The current pandemic outbreak of COVID-19 due to SARS-CoV-2 virus, affected the health care systems, health services and economy globally. Moreover, it significantly affected the health of the population worldwide. Mortality and morbidity rates are still increasing. According to WHO, as of September 2021 there have been 224180869 confirmed cases of COVID-19, including 4621173 deaths. USA, India, and Brazil are the three world's worst-hit countries. In Greece the mortality rate is at 3%. \u0000Methods: Study population included 565 patients, who were admitted at the Emergency Department and the Pathology Department of Naval and Veterans Hospital, Athens, Greece, during a period of 3,5 months. Patients’ demographic characteristics, underlying diseases, travel history, symptoms, aetiology of admission and history of contact with confirmed cases were recorded. All patients included to the study were positive for SARS-CoV-2 and characterized as COVID-19 patients. All statistical analyses were conducted using MINITAB 17. \u0000Results: Statistically significant differences in the results of albumin (marginal p-value), urea, creatinine, AST, ALT, and LDH between hospitalized and non-hospitalized patients were detected. Also, we observed statistically significant differences in the results of albumin, urea, creatinine, and ALT, between male and female patients. Moreover, patient age was statistically significant between male and female patients. The Logistic regression model of hospitalization show that statistically significant variables are ALT, LDH, age and gender. \u0000Conclusions: The rapid spreading of the new COVID-19 pandemic due to SARS-CoV-2 increased the need for the measurement of biochemical tests and the evaluation of their correlation with patient hospitalization. Biochemical monitoring of COVID-19 patients is critical for assessing disease severity and progression as well as monitoring therapeutic intervention. Several common biochemical tests have been implicated in COVID-19 infection progression, providing important prognostic information. In the present study we evaluated the test results of albumin, urea, creatinine, AST, ALT, LDH and total bilirubin in patients with COVID-19 infection.","PeriodicalId":72970,"journal":{"name":"European journal of biomedical research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72991807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}