{"title":"Discovery of MicroRNAs in Cardamom (Elettaria cardamomum Maton) under Drought Stress","authors":"N. Anjali, F. Nadiya, Jinu Thomas, K. Sabu","doi":"10.1155/2017/9507485","DOIUrl":"https://doi.org/10.1155/2017/9507485","url":null,"abstract":"MicroRNAs (miRNAs) are endogenous, 19–24-nucleotide-long, noncoding RNAs found in animals, plants, and some viruses. Drought is one of the major limiting factors that negatively affect productivity of plants. To date, nothing is known about the regulatory roles of miRNAs in response to drought stress in cardamom. Cardamom collected from the natural forest area in Therakkudi in the Idamalayar forest range was selected for the study. Total RNA was isolated from leaves and stems of control and drought treated cardamom plant, which was used for library construction. Ion torrent sequencing of the two small RNA libraries prepared from plants raised under well irrigated and drought stressed treatments, respectively, created 3,938,342 and 4,083,181 primary reads. A total of 150 conserved and 20 novel microRNAs were identified from both the control and the treated libraries. Discovery of 17 differentially expressed miRNAs under drought stress suggests that these might have been involved in various biological processes to improve plant tolerance to water stress. This study is the first report of drought responsive miRNAs and their targets in cardamom. The outcome of this research could provide insights into the miRNA based regulatory mechanisms in response to drought stress in monocot plants.","PeriodicalId":7288,"journal":{"name":"Adv. Artif. Neural Syst.","volume":"46 1","pages":"9507485:1-9507485:4"},"PeriodicalIF":0.0,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77327505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cody J. Champion, Phanidhar Kukutla, Elizabeth K. K. Glennon, Bo Wang, S. Luckhart, Jiannong Xu
{"title":"Anopheles gambiae: Metabolomic Profiles in Sugar-Fed, Blood-Fed, and Plasmodium falciparum-Infected Midgut","authors":"Cody J. Champion, Phanidhar Kukutla, Elizabeth K. K. Glennon, Bo Wang, S. Luckhart, Jiannong Xu","doi":"10.1155/2017/8091749","DOIUrl":"https://doi.org/10.1155/2017/8091749","url":null,"abstract":"The mosquito midgut is a physiological organ essential for nutrient acquisition as well as an interface that encounters various mosquito-borne pathogens. Metabolomic characterization would reveal biochemical fingerprints that are generated by various cellular processes. The metabolite profiles of the mosquito midgut will provide an overview of the biochemical events in both physiological states and the dynamic responses to pathogen infections. In this study, the midgut metabolic profiles of Anopheles gambiae mosquitoes following feeding with sugar, human blood, mouse blood, and Plasmodium falciparum-infected human blood were examined. A mass spectrometry system coupled to liquid and gas chromatography produced a time series of metabolites in the midgut at discrete conditions (sugar feeding, 24 h and 48 h post-normal blood and P. falciparum-infected blood feeding). Triplicates were included to ensure system validity. A total of 512 individual compounds were identified; 511 were assigned to 8 superpathways and 75 subpathways. The dataset can be used for further inquiry into the metabolic dynamics of sugar and blood digestion and of malaria parasite infection. The dataset is accessible at the repository Dryad.","PeriodicalId":7288,"journal":{"name":"Adv. Artif. Neural Syst.","volume":"63 1","pages":"8091749:1-8091749:49"},"PeriodicalIF":0.0,"publicationDate":"2017-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80785516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhi‐Qiang Liu, Yoke Mooi Ng, P. Tiong, Ruwaida Asyikin Abu Talip, Nornadia Jasin, V. Jong, M. Tay
{"title":"Five-Coordinate Zinc(II) Complex: Synthesis, Characterization, Molecular Structure, and Antibacterial Activities of Bis-[(E)-2-hydroxy-N′- {1-(4-methoxyphenyl)ethylidene}benzohydrazido]dimethylsulfoxidezinc(II) Complex","authors":"Zhi‐Qiang Liu, Yoke Mooi Ng, P. Tiong, Ruwaida Asyikin Abu Talip, Nornadia Jasin, V. Jong, M. Tay","doi":"10.1155/2017/7520640","DOIUrl":"https://doi.org/10.1155/2017/7520640","url":null,"abstract":"The titled Zn(II) complex was synthesized by reacting the compound (E)-2-hydroxy-N′- 1-(4-methoxyphenyl)ethylidene benzohydrazide with zinc(II) acetate dihydrate in alkaline DMSO and ethanol solution under reflux condition for 28 hours. The resulting solid was filtered and recrystallized from the mixture of ethanol and DMSO. The hydrazone Schiff base and its Zn(II) complex were characterized using 1H, 13C NMR, FTIR, UV-Vis spectroscopy, and single crystal X-ray diffraction analysis. Meanwhile, their antibacterial activities were examined using disc diffusion method. The spectral studies showed that the hydrazone Schiff base underwent keto-enol tautomerization, forming a bidentate ligand (N,O) towards Zn(II) ion. Surprisingly, on top of the two hydrazone Schiff base molecules which coordinated to the Zn metal center, an additional DMSO molecule was found attached to the Zn metal center in the crystal data, resulting in a 5-coordinate distorted trigonal bipyramidal Zn(II) complex. Both hydrazone Schiff base and its Zn(II) complexes were found to exhibit low antibacterial activity even when the concentrations were increased to 800 ppm.","PeriodicalId":7288,"journal":{"name":"Adv. Artif. Neural Syst.","volume":"10 1","pages":"7520640:1-7520640:8"},"PeriodicalIF":0.0,"publicationDate":"2017-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83411239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Glyphosate and Mancozeb on the Rhizobia Isolated from Nodules of Vicia faba L. and on Their N2-Fixation, North Showa, Amhara Regional State, Ethiopia","authors":"Birhan Aynalem, F. Assefa","doi":"10.1155/2017/5864598","DOIUrl":"https://doi.org/10.1155/2017/5864598","url":null,"abstract":"This study was designed to assess the effect of glyphosate and mancozeb on growth of Vicia faba rhizobia isolates in vitro and on their N2-fixation performance. Hence, ten isolates were isolated using plant-soil trap method from soil samples collected from farm lands. Those isolates were morphologically characterized using YEMA medium and authenticated as nodulating rhizobia using sand culture. These isolates were treated with 100, 150, and 200 μg a.e. L−1 glyphosate, 100, 150, and 200 mg L−1 mancozeb, and their combinations. The result showed that almost all isolates were affected (only 4–10% survival) at lower (100 mg L−1) concentration of mancozeb. However, 80% of isolates treated with higher concentration (200 μg a.e. L−1) of glyphosate for 72 h formed colonies on YEMA medium. Moderate (40%) isolates also showed better (31–50% and 17–45%) survival within 100 : 100 and 150 : 150 combinations of glyphosate and mancozeb, respectively. For in vivo experiment, faba bean seedlings in sand culture were inoculated with four relatively in vitro test resistant and one sensitive isolates. The inoculated isolates were treated with field recommended concentration of glyphosate, mancozeb, and combinations. Thus, experimental plants almost all showed normal (61–124 nodule plant−1) nodulation and N2-fixation (90–109%) performance as compared to the control.","PeriodicalId":7288,"journal":{"name":"Adv. Artif. Neural Syst.","volume":"29 1","pages":"5864598:1-5864598:7"},"PeriodicalIF":0.0,"publicationDate":"2017-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83800597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Balancing African Elephant Conservation with Human Well-Being in Rombo Area, Tanzania","authors":"N. Mmbaga, L. Munishi, A. Treydte","doi":"10.1155/2017/4184261","DOIUrl":"https://doi.org/10.1155/2017/4184261","url":null,"abstract":"The critical assessment of the nature and extent of human-elephant conflict (HEC) and its impact on conservation efforts are essential if we are to meet the challenges related to extinction of local population, as well as loss biodiversity. Conservationists need detailed information on HEC in areas where these challenges prevail to improve intervention in the face of limited funds/resources. We assessed the status of HEC at Rombo area over the last six years. Data based on household surveys, focus group discussions, spatiotemporal analysis of site observations, and reported incidents of damage within the last six years were mapped. Out of all HEC cases analyzed, the most were crop damage which took place at night and the damage was severe between May and July, when cereal crops were mature. In upland areas of Rombo, HEC hotspots were observed inside the protected forest plantation where local people cultivated their annual crops. Cold spots concentrated in upland areas outside the protected forest plantation dominated by settlement and agroforestry less preferred by elephants. In lowland areas, HEC hotspots were observed in village lands close to the PAs, within settlement and farmland dominated by seasonal crops. This suggests that HEC management efforts such as establishment of buffer zones should be directed around the areas adjacent to PAs and prevention should focus on cultivation of the alternative crops and farming systems that are less preferred by elephants. Our study highlights the importance of using a combination of data collection techniques to pinpoint fine-scale HEC hotspots in a highly conflict-prone location of Tanzania.","PeriodicalId":7288,"journal":{"name":"Adv. Artif. Neural Syst.","volume":"47 1","pages":"4184261:1-4184261:9"},"PeriodicalIF":0.0,"publicationDate":"2017-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86160981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Kishcha, B. Starobinets, I. Gertman, T. Ozer, P. Alpert
{"title":"Observations of Unexpected Short-Term Heating in the Uppermost Layer of the Dead Sea after a Sharp Decrease in Solar Radiation","authors":"P. Kishcha, B. Starobinets, I. Gertman, T. Ozer, P. Alpert","doi":"10.1155/2017/5810575","DOIUrl":"https://doi.org/10.1155/2017/5810575","url":null,"abstract":"The Dead Sea is one of the saltiest bodies of water in the world. Observational evidence has been obtained of unexpected short-term water heating in the 2 m uppermost layer of this hypersaline lake, following a sharp drop in solar radiation under weak winds. This was carried out using Dead Sea buoy measurements. Passing frontal cloudiness mixed with significant dust pollution over the Judean Mountains and the Dead Sea, which occurred on March 22, 2013, led to a dramatic drop in noon solar radiation from 860 W m−2 to 50 W m−2. This drop in solar radiation caused a short-term (1-hour) pronounced temperature rise in the uppermost layer of the sea down to 2 m depth. After the sharp drop in noon solar radiation, in the absence of water mixing, buoy measurements showed that the temperature rise in the uppermost layer of the Dead Sea took place for a shorter time and was more pronounced than the temperature rise under the regular diurnal solar cycle. The water heating could be explained by gravitational instability in the skin-surface layer, when the warm surface water with the increased salinity and density submerged, thereby increasing temperature in the layers below.","PeriodicalId":7288,"journal":{"name":"Adv. Artif. Neural Syst.","volume":"72 1","pages":"5810575:1-5810575:12"},"PeriodicalIF":0.0,"publicationDate":"2017-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79583988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Dai, Dong-po Wang, Ting Wang, Q. Feng, Xinqi Yang
{"title":"Analysis and Comparison of Long-Distance Pipeline Failures","authors":"L. Dai, Dong-po Wang, Ting Wang, Q. Feng, Xinqi Yang","doi":"10.1155/2017/3174636","DOIUrl":"https://doi.org/10.1155/2017/3174636","url":null,"abstract":"The analysis results of long-distance oil and gas pipeline failures are important for the industry and can be the basis of risk analysis, integrity assessment, and management improvement for pipeline operators. Through analysis and comparison of the statistical results of the United States, Europe, the UK, and PetroChina in pipeline failure frequencies, causes, consequences, similarities, and differences of pipeline management, focusing points and management effectiveness are given. Suggestions on long-distance pipeline safety technology and management in China are proposed.","PeriodicalId":7288,"journal":{"name":"Adv. Artif. Neural Syst.","volume":"4 1","pages":"3174636:1-3174636:7"},"PeriodicalIF":0.0,"publicationDate":"2017-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84437796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of a Sauce Using Gymnema sylvestre Leaves","authors":"O. Perera, P. Pavitha","doi":"10.1155/2017/5196524","DOIUrl":"https://doi.org/10.1155/2017/5196524","url":null,"abstract":"Plant derived products have attracted enormous attention due to their diverse range of biological and therapeutic properties. Gymnema sylvestre is an underutilized medicinal herb that is widely distributed in different parts of the world. It is rich in phytochemicals with antidiabetic and antiobesity properties; however, it is less popular due to its bitter taste. This study was focused on developing a sauce using leaves of Gymnema sylvestre. The most acceptable composition (17.2% water, 4.8% vinegar, 3.2% sweet potato, 2.6% chili, 2.0% sugar, 2.0% cinnamon, 2.0% cardamom, 1.8% ginger, 1.8% garlic, 1.2% onion, 1.2% salt, 0.1% citric acid, and 0.1% sodium benzoate) of the sauce was with 60% leaf extract. Final product contains 73.54% ± 1.68 moisture, 19.92% ± 2.59 carbohydrate, 2.98% ± 0.44 ash, 2.61% ± 0.58 protein, 0.88% ± 0.05 fiber, and 0.05% ± 0.01 fat. The total flavonoid content was 243.67 ± 8.57 µ moles of rutin equivalent, total phenolic content was 65.44 ± 0.51 µ moles of gallic acid equivalent, and % of inhibition of DPPH of total antioxidant capacity was 43.70 ± 0.72. Sauce formulated with 1000 ppm sodium benzoate had six weeks of shelf life. The results showed that development of a sauce from G. sylvestre leaves is an effective way of delivering the health benefits to the potential consumers.","PeriodicalId":7288,"journal":{"name":"Adv. Artif. Neural Syst.","volume":"46 1","pages":"5196524:1-5196524:5"},"PeriodicalIF":0.0,"publicationDate":"2017-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76325082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Kana, A. Fomethe, H. Fotsin, E. T. Wembe, A. Moukengue
{"title":"Complex Dynamics and Synchronization in a System of Magnetically Coupled Colpitts Oscillators","authors":"L. Kana, A. Fomethe, H. Fotsin, E. T. Wembe, A. Moukengue","doi":"10.1155/2017/5483956","DOIUrl":"https://doi.org/10.1155/2017/5483956","url":null,"abstract":"We propose the use of a simple, cheap, and easy technique for the study of dynamic and synchronization of the coupled systems: effects of the magnetic coupling on the dynamics and of synchronization of two Colpitts oscillators (wireless interaction). We derive a smooth mathematical model to describe the dynamic system. The stability of the equilibrium states is investigated. The coupled system exhibits spectral characteristics such as chaos and hyperchaos in some parameter ranges of the coupling. The numerical exploration of the dynamics system reveals various bifurcations scenarios including period-doubling and interior crisis transitions to chaos. Moreover, various interesting dynamical phenomena such as transient chaos, coexistence of solution, and multistability (hysteresis) are observed when the magnetic coupling factor varies. Theoretical reasons for such phenomena are provided and experimentally confirmed with practical measurements in a wireless transfer.","PeriodicalId":7288,"journal":{"name":"Adv. Artif. Neural Syst.","volume":"283 1","pages":"5483956:1-5483956:13"},"PeriodicalIF":0.0,"publicationDate":"2017-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80195921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ruting Jia, V. Nandikolla, G. Haggart, C. Volk, D. Tazartes
{"title":"System Performance of an Inertially Stabilized Gimbal Platform with Friction, Resonance, and Vibration Effects","authors":"Ruting Jia, V. Nandikolla, G. Haggart, C. Volk, D. Tazartes","doi":"10.1155/2017/6594861","DOIUrl":"https://doi.org/10.1155/2017/6594861","url":null,"abstract":"The research work evaluates the quality of the sensor to perform measurements and documents its effects on the performance of the system. It also evaluates if this performance changes due to the environments and other system parameters. These environments and parameters include vibration, system friction, structural resonance, and dynamic system input. The analysis is done by modeling a gimbal camera system that requires angular measurements from inertial sensors and gyros for stabilization. Overall, modeling includes models for four different types of gyros, the gimbal camera system, the drive motor, the motor rate control system, and the angle position control system. Models for friction, structural resonance, and vibration are analyzed, respectively. The system is simulated, for an ideal system, and then includes the more realistic environmental and system parameters. These simulations are run with each of the four types of gyros. The performance analysis depicts that for the ideal system; increasing gyro quality provides better system performance. However, when environmental and system parameters are introduced, this is no longer the case. There are even cases when lower quality sensors provide better performance than higher quality sensors.","PeriodicalId":7288,"journal":{"name":"Adv. Artif. Neural Syst.","volume":"11 1","pages":"6594861:1-6594861:20"},"PeriodicalIF":0.0,"publicationDate":"2017-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84158277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}