Advances in experimental medicine and biology最新文献

{"title":"Transformative Therapies for Wound Care: Insights into Tissue Engineering and Regenerative Medicine.","authors":"Mrunal Damle, Vaishali Gaikwad, Meghnad G Joshi","doi":"10.1007/5584_2025_879","DOIUrl":"https://doi.org/10.1007/5584_2025_879","url":null,"abstract":"<p><p>Wound healing is a dynamic and complex process that consists of four interconnected phases: hemostasis, inflammation, proliferation, and remodeling. This complex process is based on the coordinated actions of growth factors, cytokines, and other cellular interactions. However, conditions such as diabetes and chronic illnesses can disrupt this process and lead to nonhealing wounds or chronic ulcers. This chapter addresses the molecular and cellular mechanisms that control both normal and impaired wound healing, with emphasis on diabetic ulcers, burns, and surgical wounds. Growth factors play a critical role in wound modulation and the potential of therapeutic interventions to restore balanced healing. Advances in tissue engineering and regenerative medicine, including hydrogel-based therapies and synthetic polymers, have produced promising solutions for wound management. In addition, 3D bioprinting offers the possibility of producing personalized skin grafts and wound dressings that closely resemble the natural skin structure. Clinical trials are currently evaluating these innovative approaches' effectiveness and highlighting their potential to transform therapeutic outcomes in the treatment of chronic and complex wounds.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative Dentistry: Applications of Bioactive Materials in Dentin-Pulp Complex.","authors":"Soner Sismanoglu, Vasfiye Işık, Pınar Ercal","doi":"10.1007/5584_2025_876","DOIUrl":"https://doi.org/10.1007/5584_2025_876","url":null,"abstract":"<p><p>Tissue engineering in dentistry is revolutionizing the regeneration of dental pulp. The dental pulp is a specialized connective tissue that plays an important role in maintaining tooth health and supporting healing processes. However, exposure of the pulp to harmful factors, such as infections or trauma, can negatively impact its function, leading to inflammation, tissue necrosis, and ultimately pulp loss. As a solution to these challenges, tissue-engineered vital pulp therapies (VPTs) are emerging as an alternative to conventional root canal treatments. These therapies aim to preserve the vitality of the pulp, stimulate natural healing processes, and restore the dentin-pulp structure. Regenerative dentistry is also exploring tissue repair through innovations such as three-dimensional (3D) bioprinting, exosome-based therapies, and novel scaffold structures.This chapter explores the potential of tissue engineering in dental pulp regeneration, focusing on the role of stem cells, growth factors, scaffolds, and bioactive materials. In particular, stem cells derived from dental pulp are critical to this process due to their ability to differentiate into odontoblast-like cells and promote dentin production. The combination of these stem cells with bioactive scaffolds that release growth factors can significantly enhance the healing of pulp tissue. Furthermore, innovative materials, such as calcium silicate-based materials and bioactive glasses, have shown promising results in pulp regeneration and restorative dentin formation. While the future of these therapies is promising, challenges such as clinical application, long-term efficacy, and cost-effectiveness remain. As research advances, the importance of interdisciplinary collaboration and clinical trials will grow in overcoming these barriers.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insect Lipidomics: Advances, Applications, and Physiological Insights.","authors":"Laura Palanker Musselman, Doga Cedden, Gözde Güney, Umut Toprak","doi":"10.1007/5584_2025_878","DOIUrl":"https://doi.org/10.1007/5584_2025_878","url":null,"abstract":"<p><p>Lipidomics, a specialized branch of metabolomics, investigates the diversity and functionality of lipids in biological systems. Lipids serve crucial roles in energy storage, membrane composition, and environmental acclimation in insects, underpinning processes such as development and stress responses. Advances in analytical technologies, such as liquid chromatography-mass spectrometry (LC-MS), have enabled precise identification and quantification of lipid species, providing unprecedented insights into lipid metabolism and dynamics. Key lipid classes, including triacylglycerols and phospholipids, exhibit structural and functional versatility, adapting to environmental pressures through mechanisms like homeoviscous adaptation. These dynamic lipid responses are essential for maintaining cellular and cuticular integrity and functionality under stress. By exploring lipid diversity and adaptations, lipidomics offers valuable perspectives on insect physiology, survival strategies, and evolutionary ecology. This chapter summarizes methods used to study insect lipidomes and highlights comparative lipidomic studies that have advanced our understanding of insect biology.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative Strategies in Pediatric Dentistry: A Comprehensive Overview of Tissue Engineering from Past to Future.","authors":"Belen Şirinoğlu Çapan, Sinem Birant, Burak Gümüştaş, Pınar Ercal, Soner Sismanoglu","doi":"10.1007/5584_2025_877","DOIUrl":"https://doi.org/10.1007/5584_2025_877","url":null,"abstract":"<p><p>Tissue engineering in pediatric dentistry focuses on regenerating damaged dental tissues in children, aiming to restore their natural functions and address challenges associated with immature teeth. This field applies interdisciplinary biological principles to repair and regenerate tissues, using biomaterials, stem cells, and growth factors. Current advancements emphasize the regeneration of the dentin-pulp complex and tooth development through the use of biomaterials such as collagen, which offers structural support for cell growth and favorable interactions in tissue engineering. Additionally, various growth factors like FGF, TGF-β, BMP, and VEGF play vital roles in tissue regeneration by regulating signaling mechanisms in the dentin-pulp complex. Stem cells, particularly dental-derived ones such as DPSCs, SHEDs, and PDLSCs, have shown great potential in regenerating dental tissues in pediatric patients. These multipotent cells are capable of differentiating into various lineages, including odontoblasts, and are essential for the regeneration of both soft and hard dental tissues. While gene therapy, scaffolding techniques, and advanced technologies like three-dimensional (3D) bioprinting hold promise for tissue engineering, challenges remain in clinical implementation due to high costs and the need for further research. However, tissue engineering has already begun revolutionizing pediatric dental treatments, particularly in regenerative endodontics, and offers a minimally invasive alternative to traditional procedures like root canal treatments for young immature teeth. The future of regenerative dentistry in pediatric care lies in improving the application of stem cell-based therapies and bioactive materials to achieve complete tissue regeneration and provide more effective, personalized care for young patients.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustained Drug Delivery Systems for Age-Related Macular Degeneration: Advancements, Challenges, and Future Perspectives.","authors":"Ece Seber, Seher Yaylacı","doi":"10.1007/5584_2025_871","DOIUrl":"https://doi.org/10.1007/5584_2025_871","url":null,"abstract":"<p><p>Age-related macular degeneration (AMD) is a leading cause of vision loss among the elderly and remains a major public health challenge due to its chronic progression and high recurrence rate. Current standard treatment relies heavily on repeated intravitreal injections of anti-vascular endothelial growth factor (VEGF) agents, which impose significant burdens on patients and healthcare systems. In recent years, sustained-release drug delivery systems (SR-DDS) have emerged as promising alternatives to overcome the limitations of frequent injections and to improve therapeutic outcomes in AMD.This chapter explores the rationale, mechanisms, and current advancements in hydrogel-based platforms and implantable devices designed for the long-term delivery of anti-VEGF agents. We provide a comparative analysis of these two delivery modalities, focusing on their materials, biocompatibility, release kinetics, clinical utility, and translational potential. Furthermore, we discuss the critical role of SR-DDS in supporting personalized medicine through patient-specific dosing strategies and their ability to maintain therapeutic drug levels with minimal invasiveness.Challenges in the development and regulatory approval of SR-DDS, including manufacturing complexity, long-term safety, and economic viability, are also addressed. Finally, we highlight emerging trends in biomaterials science, precision engineering, and combination therapy strategies that may shape the next generation of drug delivery platforms for AMD.Together, these insights underscore the transformative potential of SR-DDS in redefining the clinical management of AMD by offering sustained, localized, and patient-tailored therapeutic delivery.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Complexities of Bone Implants and Stents: Factors Influencing Successful Tissue Integration.","authors":"Yelda Yüregir, Seher Yaylacı, Hakan Ceylan","doi":"10.1007/5584_2025_872","DOIUrl":"https://doi.org/10.1007/5584_2025_872","url":null,"abstract":"<p><p>Bone implants and stents are medical devices that are commonly used to treat bone and cardiovascular diseases, respectively. Both require successful integration with the surrounding tissue to achieve long-term success. Osteointegration, the process by which the implant becomes integrated with the surrounding bone, is critical to the success of bone implants, while the stent healing process involves endothelialization, re-endothelialization, and neointimal formation. The healing process of bone is complex and influenced by various factors, including the properties of the implant material, the surgical technique, and patient factors such as age and overall health. Several materials have been developed for bone implants, including metals, ceramics, and polymers. The choice of material depends on the specific application, as each material has unique properties that affect its suitability for a particular use. For example, titanium is commonly used in orthopedic implants due to its biocompatibility, strength, and ability to promote osteointegration. The healing process of stents is influenced by the materials used and the stent design. Drug-eluting stents, which release drugs to reduce restenosis, have been developed to improve the healing process. Endothelialization, the formation of a layer of endothelial cells over the stent, is critical to the prevention of restenosis. Neointimal formation, the formation of new tissue over the stent, can cause restenosis and has been a major concern with bare-metal stents. Factors that affect osteointegration and stent healing process include implant surface properties, such as roughness and topography, as well as the size, shape, and placement of the implant. In addition, patient factors such as age, overall health, and medication use can also affect the healing process. In conclusion, successful integration with the surrounding tissue is critical to the long-term success of bone implants and stents. The choice of implant material, surgical technique, and patient factors all play a role in the healing process, and ongoing research is needed to improve the design and performance of these medical devices.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Ovarian Stem Cells in Postnatal Oogenesis and Implications in Fertility Preservation.","authors":"C Benlioglu, V Turan, Ozgur Oktem","doi":"10.1007/5584_2025_869","DOIUrl":"https://doi.org/10.1007/5584_2025_869","url":null,"abstract":"<p><p>It is a long-held dogma in reproductive biology that females are born with a set nonrenewable number of oocytes in the ovary. While some animal studies challenged this dogma by demonstrating allegedly postnatal oogenesis and the presence of certain stem cell factor expression germ cell markers, their biological roles are yet to be defined. In this chapter, we revisited this issue revisit this dogma in light of most recent data. The chapter outlines the characteristics of these putative OSCs, including their morphological features, expression of germline markers (e.g., DDX4, DAZL), telomerase activity, and methods for their isolation and culture, acknowledging the ongoing debate and methodological controversies surrounding their identification, particularly the reliability of DDX4-based sorting. We critically evaluate the evidence for their capacity to differentiate into oocyte-like cells in vitro and in vivo, including experiments involving transplantation into animal models. Finally, the chapter explores the profound implications these OSCs hold for fertility preservation, especially for women facing premature ovarian insufficiency, age-related infertility, or gonadotoxic treatments. We discuss potential future applications, such as autologous OSC transplantation to restore ovarian function or in vitro generation of mature oocytes for fertilization. We highlight the significant technical and safety hurdles that must be overcome, including optimizing culture systems, ensuring genomic stability, and validating human functional competency. While the physiological role and definitive existence of functional OSCs in adult human ovaries remain contentious subjects requiring further rigorous investigation using advanced techniques like single-cell analysis and lineage tracing, the potential therapeutic promise continues to drive research in this exciting, albeit controversial, area of reproductive medicine.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Fat Body: Structure and Functions.","authors":"Patrycja Staniszewska, Aneta Strachecka","doi":"10.1007/5584_2025_874","DOIUrl":"https://doi.org/10.1007/5584_2025_874","url":null,"abstract":"<p><p>The fat body plays a vital role in the proper functioning of invertebrates, contributing to their metabolic processes and resilience. It serves as the central tissue for metabolism integrating signals, regulating molting and metamorphosis and producing hormones that govern the overall body function and immune system protein synthesis. Thanks to this multifunctionality, the fat body is responsible for the metabolism of basic compounds: lipids, carbohydrates and proteins, storing them in the form of reserves (protecting against weather conditions, starving, etc.) and \"post-trials\" metabolites. In this tissue the remodeling process takes place, which enables the metamorphosis of insects. Also, the fat body is the place of the synthesis of immune proteins, some hormones, pheromones and vitellogenin. Understanding its physiology has, therefore, become an important element of research on insects in the context of general health.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global Research Trends and Collaborative Networks in Cellular Therapy for Crohn's Disease: A Systematic Review and Bibliographic Analysis.","authors":"Tunç Akkoç","doi":"10.1007/5584_2025_866","DOIUrl":"https://doi.org/10.1007/5584_2025_866","url":null,"abstract":"<p><strong>Introduction: </strong>Crohn's disease (CD) is a chronic inflammatory condition of the gastrointestinal system characterized by periods of remission and relapse. This study aims to provide a comprehensive bibliometric analysis of CD cellular therapy research over the past 30 years.</p><p><strong>Methods: </strong>Data was accessed and downloaded from the Web of Science (WoS) database on June 18, 2024, using specific search terms related to CDs and cellular therapy, covering the years 1992-2023. The search was limited to articles and review articles in English. The data was analyzed using R Studio Bibliometric to evaluate metrics such as global and local citations, source journals, and fractionalized author contributions.</p><p><strong>Results: </strong>The analysis identified 559 documents from 258 sources, with an annual growth rate of 13.45%. The dataset included 309 original research articles and 234 review articles, contributed by 3,181 authors. A significant publication increase was observed from 2009 onward, peaking in 2019. The mean total citations per year showed notable fluctuations, with peaks in 2000 and 2007. The Cleveland Clinic Foundation, Sun Yat-Sen University, and Mayo Clinic were prominent institutions contributing to the research. The USA led publication output and citations, followed by Spain, China, Italy, and Canada.</p><p><strong>Conclusion: </strong>The management of CD has evolved significantly with advancements in cellular therapy and therapeutic strategies. Despite the growth in research and available treatment options, there remains a lack of broad consensus in clinical practice. This bibliometric analysis highlights the progress in cellular therapy for CD.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vıtro Productıon of Bovıne Blastocysts and Cryopreservatıon Technıques.","authors":"Tolga Akkoç","doi":"10.1007/5584_2025_868","DOIUrl":"https://doi.org/10.1007/5584_2025_868","url":null,"abstract":"<p><p>Recent advancements in agricultural biotechnology have opened new avenues for improving genetic gain and reproductive efficiency in livestock. In this context, in vitro embryo production (IVP) and cryopreservation technologies have emerged as strategic tools in modern cattle breeding programs. The ability to produce high-quality bovine blastocysts under laboratory conditions offers considerable advantages, including the preservation of elite genetics, increased fertility outcomes, and enhanced sustainability in animal production systems.This chapter presents a comprehensive overview of the IVP process, beginning with the collection of bovine ovaries, followed by in vitro maturation (IVM), fertilization (IVF), embryo culture (IVC), and blastocyst cryopreservation. Particular emphasis is given to the use of advanced culture media enriched with growth factors and hormones, as well as to vitrification techniques such as solid surface vitrification (SSV) and conventional methods. Comparative evaluations of post-thaw viability, expansion, and zona hatching rates demonstrate the effectiveness of optimized protocols in preserving embryo integrity. The integration of IVP with genomic selection is also highlighted as a means of accelerating genetic improvement, particularly through early oocyte recovery from prepubertal donors.Overall, this study underscores the critical role of bovine blastocyst production in the future of sustainable animal agriculture, offering both scientific and practical insights into its application in reproductive biotechnology.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}