Advances in experimental medicine and biology最新文献

{"title":"Global Research Trends and Collaborative Networks in Cellular Therapy for Crohn's Disease: A Systematic Review and Bibliographic Analysis.","authors":"Tunç Akkoç","doi":"10.1007/5584_2025_866","DOIUrl":"https://doi.org/10.1007/5584_2025_866","url":null,"abstract":"<p><strong>Introduction: </strong>Crohn's disease (CD) is a chronic inflammatory condition of the gastrointestinal system characterized by periods of remission and relapse. This study aims to provide a comprehensive bibliometric analysis of CD cellular therapy research over the past 30 years.</p><p><strong>Methods: </strong>Data was accessed and downloaded from the Web of Science (WoS) database on June 18, 2024, using specific search terms related to CDs and cellular therapy, covering the years 1992-2023. The search was limited to articles and review articles in English. The data was analyzed using R Studio Bibliometric to evaluate metrics such as global and local citations, source journals, and fractionalized author contributions.</p><p><strong>Results: </strong>The analysis identified 559 documents from 258 sources, with an annual growth rate of 13.45%. The dataset included 309 original research articles and 234 review articles, contributed by 3,181 authors. A significant publication increase was observed from 2009 onward, peaking in 2019. The mean total citations per year showed notable fluctuations, with peaks in 2000 and 2007. The Cleveland Clinic Foundation, Sun Yat-Sen University, and Mayo Clinic were prominent institutions contributing to the research. The USA led publication output and citations, followed by Spain, China, Italy, and Canada.</p><p><strong>Conclusion: </strong>The management of CD has evolved significantly with advancements in cellular therapy and therapeutic strategies. Despite the growth in research and available treatment options, there remains a lack of broad consensus in clinical practice. This bibliometric analysis highlights the progress in cellular therapy for CD.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vıtro Productıon of Bovıne Blastocysts and Cryopreservatıon Technıques.","authors":"Tolga Akkoç","doi":"10.1007/5584_2025_868","DOIUrl":"https://doi.org/10.1007/5584_2025_868","url":null,"abstract":"<p><p>Recent advancements in agricultural biotechnology have opened new avenues for improving genetic gain and reproductive efficiency in livestock. In this context, in vitro embryo production (IVP) and cryopreservation technologies have emerged as strategic tools in modern cattle breeding programs. The ability to produce high-quality bovine blastocysts under laboratory conditions offers considerable advantages, including the preservation of elite genetics, increased fertility outcomes, and enhanced sustainability in animal production systems.This chapter presents a comprehensive overview of the IVP process, beginning with the collection of bovine ovaries, followed by in vitro maturation (IVM), fertilization (IVF), embryo culture (IVC), and blastocyst cryopreservation. Particular emphasis is given to the use of advanced culture media enriched with growth factors and hormones, as well as to vitrification techniques such as solid surface vitrification (SSV) and conventional methods. Comparative evaluations of post-thaw viability, expansion, and zona hatching rates demonstrate the effectiveness of optimized protocols in preserving embryo integrity. The integration of IVP with genomic selection is also highlighted as a means of accelerating genetic improvement, particularly through early oocyte recovery from prepubertal donors.Overall, this study underscores the critical role of bovine blastocyst production in the future of sustainable animal agriculture, offering both scientific and practical insights into its application in reproductive biotechnology.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell Communication in Endometrium: Understanding and Improving Endometrial Biomarkers.","authors":"Jeevitaa Kshersagar, Mrunal N Damle, Rakesh Sharma, Meghnad G Joshi","doi":"10.1007/5584_2025_867","DOIUrl":"https://doi.org/10.1007/5584_2025_867","url":null,"abstract":"<p><p>Endometrial remodeling is a unique sequence of factors that make it receptive to implantation. Despite extensive research on several molecular components that characterize endometrial receptivity, implantation failures occur in assisted reproductive technology (ART). Therefore, implantation failure remains the \"final frontier\" of infertility. The great interest is explained by the desire to find out the etiology of implantation failure. The endometrium undergoes changes to regulate paracellular permeability across the epithelium. These transformations are associated with and regulated by associated junctional protein complexes. These multiple factors of tight junctions (TJ), adherent junctions (AJ), and gap junctions (GJ) control communication between the endometrium during implantation, highlighting how molecular architectures and interactions can regulate receptivity. In our previous study, we quantified the expression of zona occludin-1 (ZO-1), E-cadherin (E-cad), claudin-1 (Cla-1), and vascular angiogenic precursor (VAP) by immunohistochemical (IHC) staining. An overview of the current knowledge of protein expression in the endometrium during the pretreatment implantation window is provided. It then explains how protein molecules or cell-cell compounds help counteract endometrial events. A deeper understanding of the connecting molecules will be the basis for new strategies that advance implantation.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid Metabolism in Relation to Calcium Homeostasis.","authors":"Umut Toprak","doi":"10.1007/5584_2025_875","DOIUrl":"https://doi.org/10.1007/5584_2025_875","url":null,"abstract":"<p><p>Calcium (Ca²⁺) homeostasis is a critical regulator of insect cellular functions, influencing neurotransmission, muscle contraction, hormone signaling, and lipid metabolism. This chapter explores the intricate relationship between Ca²⁺ signaling and lipid metabolism, emphasizing key molecular components that mediate this interaction. Store-operated calcium entry (SOCE) mechanisms, involving sarco/endoplasmic reticulum Ca²⁺-ATPase (SERCA), inositol 1,4,5-trisphosphate receptor (IP₃R), ryanodine receptor (RyR), stromal interaction molecule (STIM), and Orai1, coordinate intracellular Ca²⁺ fluxes that regulate lipid storage, mobilization, and utilization. Other Ca²⁺-binding proteins, such as calmodulin (CaM), calcineurin (CaN), regucalcin (RgN), calreticulin (CrT), and calnexin (CnX), further modulate Ca²⁺ homeostasis and impact lipid metabolism by influencing lipolysis, lipogenesis, and lipid droplet dynamics. This chapter also highlights the role of hepatocyte-like oenocytes in lipid metabolism. These cells, analogous to mammalian hepatocytes, regulate lipid processing and mobilization during fasting, forming a metabolic axis with fat body adipocytes. While Ca²⁺ signaling is well characterized in adipocytes, its role in oenocyte lipid metabolism remains largely unexplored. However, Ca²⁺-dependent regulation of lipid metabolism in mammalian hepatocytes suggests a similar involvement in insect oenocytes. A central theme is the bidirectional relationship between Ca²⁺ homeostasis and lipid metabolism. While Ca²⁺ signaling regulates lipid accumulation and hydrolysis, impaired lipid metabolism can disrupt Ca²⁺ homeostasis. For instance, Drosophila melanogaster seipin mutants with defective lipid storage exhibit reduced SERCA activity, leading to lower ER and mitochondrial Ca²⁺ levels, which impair lipogenesis. Additionally, CaN promotes lipogenesis, whereas STIM and IP₃R serve as lipolytic regulators. This metabolic feedback loop is essential for maintaining energy balance. Understanding the Ca²⁺-lipid interplay in insects provides insights into metabolic regulation, with implications for pest management and metabolic disease research. Future studies should further investigate Ca²⁺-dependent mechanisms governing oenocyte function and systemic lipid homeostasis.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redefining GVHD Prophylaxis: The Expanding Horizon of Posttransplant Cyclophosphamide.","authors":"Taner Tan, Zehra Narlı Özdemir, Sinem Civriz Bozdağ","doi":"10.1007/5584_2025_873","DOIUrl":"https://doi.org/10.1007/5584_2025_873","url":null,"abstract":"<p><p>Posttransplant cyclophosphamide (PTCy) represents a paradigm shift in the prevention of graft-versus-host disease (GVHD) following allogeneic hematopoietic stem cell transplantation (HSCT). As a high-dose, posttransplant immunomodulatory agent, PTCy offers a unique mechanism to facilitate graft tolerance, mitigate GVHD, and preserve graft-versus-leukemia (GVL) effects. The beneficial effects of PTCy on GVHD appears to be independent of donor type, graft source, or conditioning regimen intensity. This chapter provides an in-depth analysis of the mechanisms underlying PTCy and its clinical applications in haploidentical, HLA-matched, and -mismatched HSCT, highlighting its transformative impact in transplantation medicine. PTCy is emerging as a new standard GVHD prophylaxis for all HSCT settings.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Clinical Implications of 3D Printing for Osteochondral Regeneration.","authors":"Gozde Enguven, Aysegul Tiryaki, Ayse Ceren Calikoglu Koyuncu, Oguzhan Gunduz","doi":"10.1007/5584_2025_864","DOIUrl":"https://doi.org/10.1007/5584_2025_864","url":null,"abstract":"<p><p>Osteochondral (OC) diseases, which usually occur as a result of the sedentary life brought by today's conditions, lead to a painful and uncomfortable life, especially in later ages. Although there are many non-invasive and invasive methods for the elimination of osteochondral damage, none of them provide full recovery. For this reason, approaches that include advanced tissue engineering techniques are being put forward for the permanent treatment of damage by mimicking the natural tissue architecture. This chapter highlights preclinical and clinical advancements in OC tissue regeneration mainly focused on 3D printing. First, the cellular structure and composition of OC tissue are explained. Next, current strategies for the fabrication of 3D-printed scaffold OC tissue are discussed. Finally, the clinical applications of 3D-printed scaffolds are presented.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Epigenetic Modifications in Enhancing Bone Marrow Transplantation Efficacy in Cancer.","authors":"Babak Arjmand, Fatemeh Shahrahmani, Alieh Mirzaei, Arian Emamifar, Sepideh Alavi-Moghadam","doi":"10.1007/5584_2025_870","DOIUrl":"https://doi.org/10.1007/5584_2025_870","url":null,"abstract":"<p><p>Bone marrow transplantation is an important therapeutic modality in the treatment of hematological malignancies and other hematological diseases. It is typically marred by complications like graft-versus-host disease, engraftment failure of donor cells, and recurrence of the original disease. Epigenetic modifications like DNA methylation, histone modification, and noncoding RNAs play a pivotal role in regulating gene expression and cellular function. Current advances in epigenetics studies have heightened the expectation that they have the potential to enhance the efficacy of transplantation by regulating immune response, improving donor cell engraftment, and manipulating the tumor microenvironment. The current chapter discusses the implication of epigenetic modification for the solution of the issues in bone marrow transplantation, with a special focus on how they can impact graft-versus-host disease, hematopoietic reconstitution, and relapse of cancer. By integrating epigenetic therapies and biomarkers into transplant regimens, the possibility exists to improve patient outcomes and advance personalized medicine in oncology.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome Innovations in Ophthalmology and Sjögren's Syndrome.","authors":"Kevin Y Wu, Archan Dave, Gurleen K Nirwal, Michel Giunta, Vincent D H Nguyen, Simon D Tran","doi":"10.1007/5584_2025_865","DOIUrl":"https://doi.org/10.1007/5584_2025_865","url":null,"abstract":"<p><p>Exosomes, a subset of extracellular vesicles, have emerged as potential therapeutic agents in ophthalmology due to their ability to modulate immune responses, facilitate cellular communication, and promote tissue repair. This chapter explores the potential applications of exosome-based therapies in corneal and anterior segment disorders, retinal diseases, glaucoma, and Sjögren's syndrome. In corneal disorders, mesenchymal stem cell (MSC)-derived secretomes have shown promise in accelerating wound healing, reducing fibrosis, and modulating inflammation, with hydrogel encapsulation strategies potentially enhancing their efficacy. In retinal diseases, exosomes may provide neuroprotective effects in age-related macular degeneration, diabetic retinopathy, and retinitis pigmentosa by modulating oxidative stress and inflammation. In glaucoma, secretome-based therapies could support retinal ganglion cell survival and optic nerve regeneration, though their impact on intraocular pressure via the trabecular meshwork remains uncertain. Additionally, exosomal biomarkers in aqueous humor are being investigated as potential diagnostic tools. In Sjögren's syndrome, exosomal biomarkers may facilitate earlier detection, while stem cell-derived exosomes hold promise in modulating immune responses and restoring glandular function. Despite encouraging preclinical and early clinical findings, standardization, scalability, and long-term safety must be addressed before clinical translation. Future research will focus on optimizing exosome-based therapies and exploring their feasibility for ophthalmic applications.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging the Gap Between Bone Marrow Transplantation and CAR-T Cell Therapy in Hematological Cancers.","authors":"Babak Arjmand, Fatemeh Shahrahmani, Alieh Mirzaei, Sepideh Alavi-Moghadam, Rasta Arjmand, Mostafa Rezaei-Tavirani, Seyed Mohammadamin Nejati-Niyaki, Bagher Larijani, Ghasem Janbabaei, Seied Asadollah Mousavi, Abbas Hajifathali","doi":"10.1007/5584_2025_862","DOIUrl":"https://doi.org/10.1007/5584_2025_862","url":null,"abstract":"<p><p>Bone marrow transplantation and chimeric antigen receptor T cell therapy have become two important treatment modalities for hematological malignancies, each having different mechanisms of action and therapeutic roles. This chapter focuses on the chronological development and obstacles of bone marrow transplantation, the paradigm-shifting impact of chimeric antigen receptor T cell therapy, and further integrative approaches. The complementary roles of these modalities are emphasized given their optimal timing, selection of patients, and the development of novel strategies to improve therapeutic outcomes. Challenges such as cytokine release syndrome, graft-versus-host disease, and immune suppression are critically scrutinized. The future perspectives highlight the development of synergistic approaches, refining patient-centered treatments, and addressing long-term quality-of-life concerns. It is with this aim that the discussion below might help bridge these modalities through interdisciplinary efforts toward more effective, personalized, and sustainable treatments for cancer.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination Therapies in Drug Repurposing: Personalized Approaches to Combatting Leukaemia and Multiple Myeloma.","authors":"B Monchusi, P Dube, M M Takundwa, V L Kenmogne, T Malise, D B Thimiri Govinda Raj","doi":"10.1007/5584_2025_863","DOIUrl":"https://doi.org/10.1007/5584_2025_863","url":null,"abstract":"<p><p>Despite advances in cancer research, treating malignancies remains challenging due to issues like drug resistance, disease heterogeneity, and the limited efficacy of current therapies, particularly in relapsed or refractory cases. In recent years, several drugs originally approved for non-cancer indications have shown potential in cancer treatment, demonstrating anti-proliferative, anti-metastatic, and immunomodulatory effects. Drug repurposing has shown immense promise due to well-established safety profiles and mechanisms of action of the compounds. However, the implementation is fraught with clinical, logistical, regulatory, and ethical challenges, especially in diseases such as leukaemia and multiple myeloma. This chapter examines the treatment challenges in leukaemia and multiple myeloma, focusing on the role of drug repurposing in addressing therapeutic resistance and disease variability. It highlights the potential of personalized, tailored combination therapies, using repurposed drug components, to offer more effective, targeted, and cost-efficient treatment strategies, overcoming resistance and improving patient outcomes.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}