Advances in experimental medicine and biology最新文献

{"title":"Lipid Properties and Metabolism in Response to Cold.","authors":"Thomas Enriquez, Nicholas M Teets","doi":"10.1007/5584_2024_848","DOIUrl":"https://doi.org/10.1007/5584_2024_848","url":null,"abstract":"<p><p>Temperature directly shapes insect physiology and has a preponderant effect on life history traits. Winter conditions in temperate and polar regions are especially challenging for insects. Extremely low temperatures can indeed compromise insect survival by promoting freezing of body fluids, but mild cold temperatures above 0 °C (i.e., chilling) can also lead to complex and severe physiological dysregulations. Among physiological damages due to freezing and chilling, insect lipids are one of the primary targets. As low temperatures tend to rigidify phospholipid bilayers, membrane functions are compromised in the cold. Lipid rigidification due to cold also decreases the accessibility of fat stores for metabolic enzymes, and therefore their availability for basal metabolism. These deleterious effects, combined with low food availability in winter, result in substantial nutritional challenges for overwintering insects. Consequently, lipid modifications such as homeoviscous adaptation of cell membranes, fluidity maintenance of fat reserves, cuticular lipid accumulation, and production of antifreeze glycolipids are essential components of the physiological response to cold stress. The aim of the present chapter is to present the physiological challenges caused by low temperatures, the lipid modifications linked with cold tolerance in insects, and the molecular regulation of lipid metabolism during cold exposure.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Niche-Based Perspective to Stem and Cancer Stem Cells of the Lung.","authors":"Özge Boyacıoğlu, Berfin Deniz Kalali, Ege Tongün, Petek Korkusuz","doi":"10.1007/5584_2025_858","DOIUrl":"https://doi.org/10.1007/5584_2025_858","url":null,"abstract":"<p><p>Lungs carry the principle function for the conduction and exchange of air through the primary, secondary, tertiary bronchi, bronchioles, and alveoli, resulting in the exchange of oxygen to carbon dioxide within the human tissues. Lung stem and progenitor cells enable differentiation of parenchymal and stromal elements and provide homeostasis and regeneration in the microenvironment against pulmonary diseases. Tumor-initiating cancer cells (TICs) refer to a subpopulation named as cancer stem cells (CSCs) of lung cancer exhibiting high self-renewal and proliferation capacity by Notch, Hippo, Hedgehog, and Wnt signaling pathways that leads to tumor development or recurrence. Lung cancer stem cells (LCSCs) are characterized by distinct genotypic or phenotypic alterations compared to healthy lung stem cells (LSCs) that provide a potential target to treat lung cancer. Therefore, understanding the cascades responsible for the transformation of healthy to CSCs is essential to develop new targeted therapy approaches. In this chapter, we precisely highlight the latest researches on LSCs and CSCs, key signaling mechanisms within the perspective of novel targeted therapy strategies.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small Molecules and Epigenetic Modifiers in Facilitating Pancreatic β-cell Formation: A Comprehensive Insight.","authors":"Naveen Raj, Asmita Karmakar, Gloria Narayan, Rajkumar P Thummer","doi":"10.1007/5584_2025_859","DOIUrl":"https://doi.org/10.1007/5584_2025_859","url":null,"abstract":"<p><p>Diabetes mellitus, arising due to inadequate insulin release or insulin resistance, can be addressed through β-cell replacement therapy. Given the limited availability of islet cadaveric donors, alternative strategies such as differentiation of stem cells into pancreatic β-cells or direct reprogramming of somatic cells into pancreatic β-cells are emerging as viable options. This chapter elucidates the pivotal role of small molecules and associated signaling pathways in in vivo pancreatic organogenesis, allowing their emulation in vitro to facilitate pancreatic development. Small molecules exhibit distinct advantages, such as cell-permeability and non-immunogenic properties, thereby generating efficient functional β-like cells. Recent investigations highlight alterations in epigenetic marks unique to pancreatic β-cells during cellular reprogramming and diabetes pathogenesis. The study further delineates the distinctive histone modifications and DNA methylation within pancreatic β-cells, underscoring their contributions to pancreas development.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Art of Neuroregeneration De Novo and In Situ.","authors":"Eumorphia Remboutsika","doi":"10.1007/5584_2025_856","DOIUrl":"https://doi.org/10.1007/5584_2025_856","url":null,"abstract":"<p><p>Neuroregeneration refers to the ability of the nervous system to repair or regenerate neural components subsequently to spinal cord and traumatic brain injuries, peripheral nerve damage, and neurodegenerative diseases. Here, we discuss lead effectors of the healing process, neural stem cells, and non-invasive physical methods, for neuroregeneration de novo and in situ.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Regenerative Medicine, Cell Therapy, and 3D Bioprinting for Glaucoma and Retinal Diseases.","authors":"Kevin Y Wu, Rahma M Osman, Obinna Esomchukwu, Michael Marchand, Bich H Nguyen, Simon D Tran","doi":"10.1007/5584_2025_854","DOIUrl":"https://doi.org/10.1007/5584_2025_854","url":null,"abstract":"<p><p>Regenerative medicine, cell therapy, and 3D bioprinting represent promising advancements in addressing retinal and glaucomatous diseases. These conditions, including diabetic retinopathy (DR), age-related macular degeneration (AMD), inherited retinal degenerations (IRDs), and glaucomatous optic neuropathy, have complex pathophysiologies that involve neurodegeneration, oxidative stress, and vascular dysfunction. Despite significant progress in conventional therapies, including anti-VEGF injections, laser photocoagulation, and intraocular pressure (IOP)-lowering interventions, these approaches remain limited in reversing disease progression and restoring lost visual function.This chapter explores the potential of emerging regenerative therapies to fill these critical gaps. For retinal diseases, cell replacement strategies using human embryonic stem cells (hESCs), induced pluripotent stem cells (iPSCs), and mesenchymal stem cells (MSCs) have demonstrated encouraging outcomes in clinical trials, though challenges in delivery and long-term integration persist. Similarly, neuroprotective strategies and the use of retinal progenitor cells hold promise for preserving and restoring vision in degenerative retinal conditions. Advances in 3D bioprinting and retinal organoids further augment these efforts, offering innovative tools for disease modeling and therapy development.In glaucoma, regenerative approaches targeting trabecular meshwork (TM) dysfunction and retinal ganglion cell (RGC) loss are gaining traction. Stem cell-based therapies have shown potential in restoring TM functionality and providing neuroprotection, while innovative delivery systems and bioengineered platforms aim to enhance therapeutic efficacy and safety.This chapter provides an overview of the evolving landscape of regenerative therapies for retinal and glaucomatous diseases, highlighting current advancements, ongoing challenges, and future directions in the field. These approaches, while still emerging, hold the potential to transform the management of these complex ocular diseases.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Regenerative Medicine, Cell Therapy, and 3D Bioprinting for Corneal, Oculoplastic, and Orbital Surgery.","authors":"Kevin Y Wu, Natalie Kearn, Doanh Truong, Mazen Y Choulakian, Simon D Tran","doi":"10.1007/5584_2025_855","DOIUrl":"https://doi.org/10.1007/5584_2025_855","url":null,"abstract":"<p><p>Advances in regenerative medicine, cell therapy, and 3D bioprinting are reshaping the landscape of ocular surgery, offering innovative approaches to address complex conditions affecting the cornea, ocular adnexal structures, and the orbit. These technologies hold the potential to enhance treatment precision, improve functional outcomes, and address limitations in traditional surgical and therapeutic interventions.The cornea, as the eye's primary refractive and protective barrier, is particularly well-suited for regenerative approaches due to its avascular and immune-privileged nature. Cell-based therapies, including limbal stem cell transplantation as well as stromal keratocyte and corneal endothelial cell regeneration, are being investigated for their potential to restore corneal clarity and function in conditions such as limbal stem cell deficiency, keratoconus, and endothelial dysfunction. Simultaneously, 3D bioprinting technologies are enabling the development of biomimetic corneal constructs, potentially addressing the global shortage of donor tissues and facilitating personalized surgical solutions.In oculoplastic and orbital surgery, regenerative strategies and cell therapies are emerging as possible alternatives to conventional approaches for conditions such as eyelid defects, meibomian gland dysfunction, and Graves' orbitopathy. Stem cell-based therapies and bioengineered scaffolds are showing potential in restoring lacrimal glands' function as well as reconstructing complex ocular adnexal and orbital structures. Moreover, 3D-printed orbital implants and scaffolds offer innovative solutions for repairing traumatic, post-tumor resection, and congenital defects, with the potential for improved biocompatibility and precision.Molecular and gene-based therapies, including exosome delivery systems, nanoparticle-based interventions, and gene-editing techniques, are expanding the therapeutic arsenal for ophthalmic disorders. These approaches aim to enhance the efficacy of regenerative treatments by addressing underlying pathophysiological mechanisms of diseases. This chapter provides an overview of these advancements and the challenges of translating laboratory discoveries into effective therapies in clinical practice.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organoids as 3D Models for Studying Exogenous Mitochondrial Transplantation.","authors":"Ismail Eş, Oner Ulger","doi":"10.1007/5584_2025_857","DOIUrl":"https://doi.org/10.1007/5584_2025_857","url":null,"abstract":"<p><p>Mitochondria play a critical role in cellular communication, cell proliferation, and apoptosis, which make them essential to maintaining cellular health. Recently, mitochondrial transplantation has emerged as a promising therapeutic approach to treat conditions such as ischemia, neurodegenerative diseases, and cardiovascular disorders by restoring mitochondrial function in damaged cells. Despite its potential, understanding mitochondrial behavior in vivo remains challenging; however, organoid models, which are three-dimensional structures derived from stem cells that mimic human tissues, offer a solution to study mitochondrial function and transplantation strategies under controlled conditions. These models are particularly necessary in studies, as they can replicate disease conditions and consequently enable researchers to investigate mitochondrial dynamics and therapeutic integration. Developing organoid systems optimized for mitochondrial transplantation requires exploring factors that influence mitochondrial uptake, refining transplantation strategies, and understanding their role in cellular regeneration in order to advance in the field of mitochondrial research.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid Metabolism in Diapause.","authors":"Umut Toprak, Nicholas M Teets, Doga Cedden, Gözde Güney","doi":"10.1007/5584_2025_850","DOIUrl":"https://doi.org/10.1007/5584_2025_850","url":null,"abstract":"<p><p>Organisms living in temperate and polar environments encounter seasonal fluctuations that entail changes in temperature, resource availability, and biotic interactions. Thus, adaptations for synchronizing the life cycle with essential resources and persisting through unfavorable conditions are critical. Diapause, a programmed period of developmental arrest and metabolic depression, is widely used by insects to survive winter and synchronize the life cycle. In some cases, insects spend over half the year (or in some cases, multiple years) in a nonfeeding diapause state. Thus, diapause is energetically challenging, and insects accumulate surplus energy stores and/or suppress metabolism to make it through the winter. As the most energy-dense, and often most abundant, energy reserve in insects, lipids play a central role in diapause energetics. In this chapter, we provide an overview of lipid metabolism in the context of diapause. First, as this is the only chapter in this book that covers diapause, we present some of the general features of diapause. We then discuss the role of lipids as an essential energy store during diapause, focusing on patterns of lipid accumulation before diapause and patterns of utilization during diapause. In the next section, we outline some other roles of lipids during diapause in addition to their role as an energy store. Finally, we end the chapter by discussing the molecular regulation of lipid metabolism in diapause, which has received increased attention in recent years.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insect Flight and Lipid Metabolism: Beyond the Classic Knowledge.","authors":"Leonardo L Fruttero, Jimena Leyria, Lilián E Canavoso","doi":"10.1007/5584_2024_849","DOIUrl":"https://doi.org/10.1007/5584_2024_849","url":null,"abstract":"<p><p>Insects are the most successful animal group by various ecological and evolutionary metrics, including species count, adaptation diversity, biomass, and environmental influence. This book delves into the underlying reasons behind insects' dominance on Earth. Lipids play pivotal roles in insect biology, serving as fuel for physiological processes, signaling molecules, and structural components of biomembranes and providing waterproofing against dehydration, among other functions. The study of insect flight has been instrumental in advancing our understanding of insect metabolism, with the migratory locust (Locusta migratoria) and the tobacco hornworm (Manduca sexta) serving as prominent models. Throughout the 1980s and 1990s, numerous studies shed light on the role of adipokinetic hormone (AKH), a crucial neuropeptide in lipid mobilization, to support the extraordinary energy demands of insect flight. Remarkably, AKH was the first identified peptide hormone in insects. These pioneering works linking lipids and flight laid the groundwork for subsequent research characterizing the physiological roles of other neuroendocrine factors in energy substrate mobilization across diverse insect species. However, in the omics era, one may be surprised by the limited understanding of the complex cascade of events governing lipid supply to insect flight muscles. Thus, this chapter aims to provide a concise overview of the evolutionary significance of insect flight, emphasizing key advancements that expand our classical knowledge in this field. Ultimately, we hope this chapter serves as a modest tribute to the pioneering researchers of one of the most captivating areas in insect biology, inspiring further exploration into the myriad roles of lipids in insect biology.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genes as Genome Stabilizers in Pluripotent Stem Cells.","authors":"Asmita Karmakar, Allan Blessing Harison Raj Augustine, Rajkumar P Thummer","doi":"10.1007/5584_2025_853","DOIUrl":"https://doi.org/10.1007/5584_2025_853","url":null,"abstract":"<p><p>Pluripotent stem cells, comprising embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are characterized by their self-renewal capacity and the ability to differentiate into cells of all three germ layers of an adult animal. Out of the two, iPSCs are generated through the reprogramming of somatic cells by inducing a pluripotency-specific transcriptional program. This process requires a resetting of the somatic cell genome to a pluripotent cell-specific genome, resulting in cellular stress at genomic, epigenetic, and transcriptional levels. Notably, in contrast to the predominant compact and inactive organization of chromatin in somatic cells, the chromatin in ESCs and iPSCs is open. Furthermore, maintaining a pluripotent state needs a plethora of changes in the genetic landscape of the cells. Here, we attempt to elucidate how certain genes safeguard genomic stability in ESCs and iPSCs, aiding in the complex cellular mechanisms that regulate self-renewal, pluripotency, and somatic reprogramming.</p>","PeriodicalId":7270,"journal":{"name":"Advances in experimental medicine and biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}