bioRxiv : the preprint server for biology最新文献_第9页

Direct detection of deformation modes on varying length scales in active biopolymer networks. 直接检测活性生物聚合物网络中不同长度尺度上的变形模式。

bioRxiv : the preprint server for biology Pub Date : 2025-01-26 DOI: 10.1101/2023.05.15.540780

Samantha Stam, Margaret L Gardel, Kimberly L Weirich

{"title":"Direct detection of deformation modes on varying length scales in active biopolymer networks.","authors":"Samantha Stam, Margaret L Gardel, Kimberly L Weirich","doi":"10.1101/2023.05.15.540780","DOIUrl":"10.1101/2023.05.15.540780","url":null,"abstract":"Correlated flows and forces that emerge from active matter orchestrate complex processes such as shape regulation and deformations in biological cells and tissues. The active materials central to cellular mechanics are cytoskeletal networks, where molecular motor activity drives deformations and remodeling. Here, we investigate deformation modes in contractile actin networks driven by the molecular motor myosin II through quantitative fluorescence microscopy. We examine the deformation anisotropy at different length scales in networks of sparsely cross-linked and bundled actin. In sparsely cross-linked networks, we find myosin-dependent biaxial buckling modes across length scales. Interestingly, both long and short-wavelength buckling may contribute to network contractility. In cross-linked bundled networks, uniaxial contraction predominates on long length scales, while the uniaxial or biaxial nature of the deformation depends on bundle microstructure at shorter length scales. The anisotropy of deformations may provide insight to the mechanical origins of contractility in actin networks and regulation of collective behavior in a variety of active materials.","PeriodicalId":72407,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/55/eb/nihpp-2023.05.15.540780v1.PMC10245561.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9638838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial periodicity in grid cell firing is explained by a neural sequence code of 2-D trajectories. 网格单元发射的空间周期性由二维轨迹的神经序列代码来解释。

bioRxiv : the preprint server for biology Pub Date : 2025-01-25 DOI: 10.1101/2023.05.30.542747

R G Rebecca, Giorgio A Ascoli, Nate M Sutton, Holger Dannenberg

引用次数: 0

QPPLab: A generally applicable software package for detecting, analyzing, and visualizing large-scale quasiperiodic spatiotemporal patterns (QPPs) of brain activity. QPPLab：一个普遍适用的软件包，用于检测、分析和可视化大脑活动的大规模准周期时空模式（QPP）。

bioRxiv : the preprint server for biology Pub Date : 2025-01-24 DOI: 10.1101/2023.09.25.559086

Nan Xu, Behnaz Yousefi, Nmachi Anumba, Theodore J LaGrow, Xiaodi Zhang, Shella Keilholz

{"title":"QPPLab: A generally applicable software package for detecting, analyzing, and visualizing large-scale quasiperiodic spatiotemporal patterns (QPPs) of brain activity.","authors":"Nan Xu, Behnaz Yousefi, Nmachi Anumba, Theodore J LaGrow, Xiaodi Zhang, Shella Keilholz","doi":"10.1101/2023.09.25.559086","DOIUrl":"10.1101/2023.09.25.559086","url":null,"abstract":"Quasi-periodic patterns (QPPs) are prominent spatiotemporal brain dynamics observed in functional neuroimaging data, reflecting the alternation of high and low activity across brain regions and their propagation along cortical gradients. QPPs have been linked to neural processes such as attention, arousal fluctuations, and cognitive function. Despite their significance, existing QPP analysis tools are limited by study-specific parameters and complex workflows. To address these challenges, we present QPPLab , an open-source MATLAB-based toolbox for detecting, analyzing, and visualizing QPPs from fMRI time series. QPPLab integrates correlation-based iterative algorithms, supports customizable parameter settings, and features automated workflows to simplify analysis. Processing times vary depending on dataset size and the selected mode, with the fast detection mode completing analyses that can be 4-6 times faster than the robust detection mode. Results include spatiotemporal templates of QPPs, sliding correlation time courses, and functional connectivity maps. By reducing manual parameter adjustments and providing user-friendly tools, QPPLab enables researchers to efficiently study QPPs across diverse datasets and species, advancing our understanding of intrinsic brain dynamics.","PeriodicalId":72407,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10557593/pdf/nihpp-2023.09.25.559086v1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41172029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stratification of enterochromaffin cells by single-cell expression analysis. 通过单细胞表达分析对肠嗜铬细胞进行分层。

bioRxiv : the preprint server for biology Pub Date : 2025-01-23 DOI: 10.1101/2023.08.24.554649

Yan Song, Linda J Fothergill, Kari S Lee, Brandon Y Liu, Ada Koo, Mark Perelis, Shanti Diwakarla, Brid Callaghan, Jie Huang, Jill Wykosky, John B Furness, Gene W Yeo

{"title":"Stratification of enterochromaffin cells by single-cell expression analysis.","authors":"Yan Song, Linda J Fothergill, Kari S Lee, Brandon Y Liu, Ada Koo, Mark Perelis, Shanti Diwakarla, Brid Callaghan, Jie Huang, Jill Wykosky, John B Furness, Gene W Yeo","doi":"10.1101/2023.08.24.554649","DOIUrl":"10.1101/2023.08.24.554649","url":null,"abstract":"Dynamic interactions between gut mucosal cells and the external environment are essential to maintain gut homeostasis. Enterochromaffin (EC) cells transduce both chemical and mechanical signals and produce 5-hydroxytryptamine (5-HT) to mediate disparate physiological responses. However, the molecular and cellular basis for functional diversity of ECs remains to be adequately defined. Here, we integrated single-cell transcriptomics with spatial image analysis to identify fourteen EC clusters that are topographically organized along the gut. Subtypes predicted to be sensitive to the chemical environment and mechanical forces were identified that express distinct transcription factors and hormones. A Piezo2 + population in the distal colon was endowed with a distinctive neuronal signature. Using a combination of genetic, chemogenetic and pharmacological approaches, we demonstrated Piezo2 + ECs are required for normal colon motility. Our study constructs a molecular map for ECs and offers a framework for deconvoluting EC cells with pleiotropic functions.","PeriodicalId":72407,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/67/34/nihpp-2023.08.24.554649v1.PMC10473706.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10178778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kiwa is a bacterial membrane-embedded defence supercomplex activated by phage-induced membrane changes. Kiwa是一种由噬菌体诱导的膜变化激活的细菌膜嵌入防御超复合体。

bioRxiv : the preprint server for biology Pub Date : 2025-01-23 DOI: 10.1101/2023.02.26.530102

Zhiying Zhang, Thomas C Todeschini, Yi Wu, Roman Kogay, Ameena Naji, Joaquin Cardenas Rodriguez, Rupavidhya Mondi, Daniel Kaganovich, David W Taylor, Jack P K Bravo, Marianna Teplova, Triana Amen, Eugene V Koonin, Dinshaw J Patel, Franklin L Nobrega

{"title":"Kiwa is a bacterial membrane-embedded defence supercomplex activated by phage-induced membrane changes.","authors":"Zhiying Zhang, Thomas C Todeschini, Yi Wu, Roman Kogay, Ameena Naji, Joaquin Cardenas Rodriguez, Rupavidhya Mondi, Daniel Kaganovich, David W Taylor, Jack P K Bravo, Marianna Teplova, Triana Amen, Eugene V Koonin, Dinshaw J Patel, Franklin L Nobrega","doi":"10.1101/2023.02.26.530102","DOIUrl":"10.1101/2023.02.26.530102","url":null,"abstract":"Bacteria and archaea deploy diverse, sophisticated defence systems to counter virus infection, yet many immunity mechanisms remain poorly understood. Here, we characterise the Kiwa defence system as a membrane-associated supercomplex that senses changes in the membrane induced by phage infection and plasmid conjugation. This supercomplex, comprising KwaA tetramers bound to KwaB dimers, as its basic repeating unit, detects structural stress via KwaA, activating KwaB, which binds ejected phage DNA through its DUF4868 domain, stalling phage DNA replication forks and thus disrupting replication and late transcription. We show that phage-encoded DNA mimic protein Gam, which inhibits RecBCD, also targets Kiwa through KwaB recognition. However, Gam binding to one defence system precludes its inhibition of the other. These findings reveal a distinct mechanism of bacterial immune coordination, where sensing of membrane disruptions and inhibitor partitioning enhance protection against phages and plasmids.","PeriodicalId":72407,"journal":{"name":"bioRxiv : the preprint server for biology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90459837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular Lactate Dynamics in Drosophila Glutamatergic Neurons. 细胞内乳酸动态揭示果蝇谷氨酸能神经元代谢的多样性

bioRxiv : the preprint server for biology Pub Date : 2025-01-23 DOI: 10.1101/2024.02.26.582095

Matthew S Price, Elham Rastegari, Richa Gupta, Katie Vo, Travis I Moore, Kartik Venkatachalam

{"title":"Intracellular Lactate Dynamics in Drosophila Glutamatergic Neurons.","authors":"Matthew S Price, Elham Rastegari, Richa Gupta, Katie Vo, Travis I Moore, Kartik Venkatachalam","doi":"10.1101/2024.02.26.582095","DOIUrl":"10.1101/2024.02.26.582095","url":null,"abstract":"Rates of lactate production and consumption reflect the metabolic state of many cell types, including neurons. Here, we investigate the effects of nutrient deprivation on lactate dynamics in Drosophila glutamatergic neurons by leveraging the limiting effects of the diffusion barrier surrounding cells in culture. We found that neurons constitutively consume lactate when availability of trehalose, the glucose disaccharide preferred by insects, is limited by the diffusion barrier. Acute mechanical disruption of the barrier reduced this reliance on lactate. Through kinetic modeling and experimental validation, we demonstrate that neuronal lactate consumption rates correlate inversely with their mitochondrial density. Further, we found that lactate levels in neurons exhibited temporal correlations that allowed prediction of cytosolic lactate dynamics after the disruption of the diffusion barrier from pre-perturbation lactate fluctuations. Collectively, our findings reveal the influence of diffusion barriers on neuronal metabolic preferences, and demonstrate the existence of temporal correlations between lactate dynamics under conditions of nutrient deprivation and those evoked by the subsequent restoration of nutrient availability.","PeriodicalId":72407,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10925175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolating Small Extracellular Vesicles from Small Volumes of Blood Plasma using size exclusion chromatography and density gradient ultracentrifugation: A Comparative Study. 用大小排斥色谱法和密度梯度超离心分离法从小体积血浆中分离细胞外小泡:一项比较研究。

bioRxiv : the preprint server for biology Pub Date : 2025-01-22 DOI: 10.1101/2023.10.30.564707

Fang Kong, Megha Upadya, Andrew See Weng Wong, Rinkoo Dalan, Ming Dao

{"title":"Isolating Small Extracellular Vesicles from Small Volumes of Blood Plasma using size exclusion chromatography and density gradient ultracentrifugation: A Comparative Study.","authors":"Fang Kong, Megha Upadya, Andrew See Weng Wong, Rinkoo Dalan, Ming Dao","doi":"10.1101/2023.10.30.564707","DOIUrl":"10.1101/2023.10.30.564707","url":null,"abstract":"Small extracellular vesicles (sEVs) are heterogeneous biological vesicles released by cells under both physiological and pathological conditions. Due to their potential as valuable diagnostic and prognostic biomarkers in human blood, there is a pressing need to develop effective methods for isolating high-purity sEVs from the complex milieu of blood plasma, which contains abundant plasma proteins and lipoproteins. Size exclusion chromatography (SEC) and density gradient ultracentrifugation (DGUC) are two commonly employed isolation techniques that have shown promise in addressing this challenge. In this study, we aimed to determine the optimal combination and sequence of SEC and DGUC for isolating sEVs from small plasma volumes, in order to enhance both the efficiency and purity of the resulting isolates. To achieve this, we compared sEV isolation using two combinations: SEC-DGUC and DGUC-SEC, from unit volumes of 500 μl plasma. Both protocols successfully isolated high-purity sEVs; however, the SEC-DGUC combination yielded higher sEV protein and RNA content. We further characterized the isolated sEVs obtained from the SEC-DGUC protocol using flow cytometry and mass spectrometry to assess their quality and purity. In conclusion, the optimized SEC-DGUC protocol is efficient, highly reproducible, and well-suited for isolating high-purity sEVs from small blood volumes.","PeriodicalId":72407,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10634961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92157784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excess neonatal testosterone causes male-specific social and fear memory deficits in wild-type mice. 在野生型小鼠中，过量的新生儿睾酮会导致雄性特定的社交和恐惧记忆缺陷。

bioRxiv : the preprint server for biology Pub Date : 2025-01-21 DOI: 10.1101/2023.10.18.562939

Pravda Quiñones-Labernik, Kelsey L Blocklinger, Matthew R Bruce, Sarah L Ferri

{"title":"Excess neonatal testosterone causes male-specific social and fear memory deficits in wild-type mice.","authors":"Pravda Quiñones-Labernik, Kelsey L Blocklinger, Matthew R Bruce, Sarah L Ferri","doi":"10.1101/2023.10.18.562939","DOIUrl":"10.1101/2023.10.18.562939","url":null,"abstract":"Neurodevelopmental disorders disproportionately affect males compared to females. The biological mechanisms of this male susceptibility or female protection have not been identified. There is evidence that fetal/neonatal gonadal hormones, which play a pivotal role in many aspects of development, may contribute. Here, we investigate the effects of excess testosterone during a critical period of sex-specific brain organization on social approach and fear learning behaviors in C57BL/6J wild-type mice. Male, but not female, mice treated with testosterone on the day of birth (PN0) exhibited decreased social approach as juveniles and decreased contextual fear memory as adults, compared to vehicle-treated controls. These deficits were not driven by anxiety-like behavior or changes in locomotion or body weight. Mice treated with the same dose of testosterone on postnatal day 18 (PN18), which is outside of the critical period of brain masculinization, did not demonstrate impairments compared to the vehicle group. These findings indicate that excess testosterone during a critical period of early development, but not shortly after, induces long-term deficits relevant to the male sex bias in neurodevelopmental disorders.","PeriodicalId":72407,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71415623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The metabolic cofactor Coenzyme A enhances alternative macrophage activation via MyD88-linked signaling. 代谢辅因子辅酶 A 可通过与 MyD88 相关的信号传导增强巨噬细胞的替代性活化。

bioRxiv : the preprint server for biology Pub Date : 2025-01-20 DOI: 10.1101/2024.03.28.587096

Anthony E Jones, Amy Rios, Neira Ibrahimovic, Carolina Chavez, Nicholas A Bayley, Andréa B Ball, Wei Yuan Hsieh, Alessandro Sammarco, Amber R Bianchi, Angel A Cortez, Thomas G Graeber, Alexander Hoffmann, Steven J Bensinger, Ajit S Divakaruni

{"title":"The metabolic cofactor Coenzyme A enhances alternative macrophage activation via MyD88-linked signaling.","authors":"Anthony E Jones, Amy Rios, Neira Ibrahimovic, Carolina Chavez, Nicholas A Bayley, Andréa B Ball, Wei Yuan Hsieh, Alessandro Sammarco, Amber R Bianchi, Angel A Cortez, Thomas G Graeber, Alexander Hoffmann, Steven J Bensinger, Ajit S Divakaruni","doi":"10.1101/2024.03.28.587096","DOIUrl":"10.1101/2024.03.28.587096","url":null,"abstract":"Metabolites and metabolic co-factors can shape the innate immune response, though the pathways by which these molecules adjust inflammation remain incompletely understood. Here we show that the metabolic cofactor Coenzyme A (CoA) enhances IL-4 driven alternative macrophage activation [m(IL-4)] in vitro and in vivo. Unexpectedly, we found that perturbations in intracellular CoA metabolism did not influence m(IL-4) differentiation. Rather, we discovered that exogenous CoA provides a weak TLR4 signal which primes macrophages for increased receptivity to IL-4 signals and resolution of inflammation via MyD88. Mechanistic studies revealed MyD88-linked signals prime for IL-4 responsiveness, in part, by reshaping chromatin accessibility to enhance transcription of IL-4-linked genes. The results identify CoA as a host metabolic co-factor that influences macrophage function through an extrinsic TLR4-dependent mechanism, and suggests that damage-associated molecular patterns (DAMPs) can prime macrophages for alternative activation and resolution of inflammation.","PeriodicalId":72407,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10996702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140859283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conditioning electrical stimulation fails to enhance sympathetic axon regeneration. 调节电刺激不能增强交感轴突的再生。

bioRxiv : the preprint server for biology Pub Date : 2025-01-20 DOI: 10.1101/2023.02.03.527071

Tina Tian, Kevin Patel, David Kim, HaoMin SiMa, Alandrea R Harris, Jordan N Owyoung, Patricia J Ward

{"title":"Conditioning electrical stimulation fails to enhance sympathetic axon regeneration.","authors":"Tina Tian, Kevin Patel, David Kim, HaoMin SiMa, Alandrea R Harris, Jordan N Owyoung, Patricia J Ward","doi":"10.1101/2023.02.03.527071","DOIUrl":"10.1101/2023.02.03.527071","url":null,"abstract":"Peripheral nerve injuries are common, and there is a critical need for the development of novel treatments to complement surgical repair. Conditioning electrical stimulation (CES) is a novel variation of the well-studied perioperative electrical stimulation treatment paradigm. CES is a clinically attractive alternative because of its ability to be performed at the bedside prior to a scheduled nerve repair surgery. Although 60 minutes of CES has been shown to enhance motor and sensory axon regeneration, the effects of CES on sympathetic regeneration are unknown. We investigated how two clinically relevant CES paradigms (10 minutes and 60 minutes) impact sympathetic axon regeneration and distal target reinnervation. Our results indicate that the growth of sympathetic axons is inhibited by CES at acute time points, and at a longer survival time point post-injury, there is no difference between sham CES and the CES groups. We conclude sympathetic axons may retain some regenerative ability, but no enhancement is exhibited after CES, which may be accounted for by the inability of the electrical stimulation paradigm to recruit the small-caliber sympathetic axons into activity. Furthermore, 10-minute CES did not enhance motor and sensory regeneration with a direct repair, and neither 60-minute nor 10-minute CES enhanced motor and sensory regeneration through a graft. Further studies will be needed to optimize electrical stimulation parameters to enhance the regeneration of all neuron types.","PeriodicalId":72407,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9915730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10692145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0