Biophysics reviews最新文献_第5页

In vitro skeletal muscle models for type 2 diabetes. 2 型糖尿病的体外骨骼肌模型。

Biophysics reviews Pub Date : 2022-09-01 Epub Date: 2022-09-13 DOI: 10.1063/5.0096420

Christina Y Sheng, Young Hoon Son, Jeongin Jang, Sung-Jin Park

{"title":"In vitro skeletal muscle models for type 2 diabetes.","authors":"Christina Y Sheng, Young Hoon Son, Jeongin Jang, Sung-Jin Park","doi":"10.1063/5.0096420","DOIUrl":"10.1063/5.0096420","url":null,"abstract":"Type 2 diabetes mellitus, a metabolic disorder characterized by abnormally elevated blood sugar, poses a growing social, economic, and medical burden worldwide. The skeletal muscle is the largest metabolic organ responsible for glucose homeostasis in the body, and its inability to properly uptake sugar often precedes type 2 diabetes. Although exercise is known to have preventative and therapeutic effects on type 2 diabetes, the underlying mechanism of these beneficial effects is largely unknown. Animal studies have been conducted to better understand the pathophysiology of type 2 diabetes and the positive effects of exercise on type 2 diabetes. However, the complexity of in vivo systems and the inability of animal models to fully capture human type 2 diabetes genetics and pathophysiology are two major limitations in these animal studies. Fortunately, in vitro models capable of recapitulating human genetics and physiology provide promising avenues to overcome these obstacles. This review summarizes current in vitro type 2 diabetes models with focuses on the skeletal muscle, interorgan crosstalk, and exercise. We discuss diabetes, its pathophysiology, common in vitro type 2 diabetes skeletal muscle models, interorgan crosstalk type 2 diabetes models, exercise benefits on type 2 diabetes, and in vitro type 2 diabetes models with exercise.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"3 3","pages":"031306"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478902/pdf/BRIEIM-000003-031306_1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10632609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myocardial infarction from a tissue engineering and regenerative medicine point of view: A comprehensive review on models and treatments. 组织工程和再生医学视角下的心肌梗死:模型和治疗方法综述。

Biophysics reviews Pub Date : 2022-09-01 DOI: 10.1063/5.0093399

Gozde Basara, Gokhan Bahcecioglu, S Gulberk Ozcebe, Bradley W Ellis, George Ronan, Pinar Zorlutuna

{"title":"Myocardial infarction from a tissue engineering and regenerative medicine point of view: A comprehensive review on models and treatments.","authors":"Gozde Basara, Gokhan Bahcecioglu, S Gulberk Ozcebe, Bradley W Ellis, George Ronan, Pinar Zorlutuna","doi":"10.1063/5.0093399","DOIUrl":"https://doi.org/10.1063/5.0093399","url":null,"abstract":"In the modern world, myocardial infarction is one of the most common cardiovascular diseases, which are responsible for around 18 million deaths every year or almost 32% of all deaths. Due to the detrimental effects of COVID-19 on the cardiovascular system, this rate is expected to increase in the coming years. Although there has been some progress in myocardial infarction treatment, translating pre-clinical findings to the clinic remains a major challenge. One reason for this is the lack of reliable and human representative healthy and fibrotic cardiac tissue models that can be used to understand the fundamentals of ischemic/reperfusion injury caused by myocardial infarction and to test new drugs and therapeutic strategies. In this review, we first present an overview of the anatomy of the heart and the pathophysiology of myocardial infarction, and then discuss the recent developments on pre-clinical infarct models, focusing mainly on the engineered three-dimensional cardiac ischemic/reperfusion injury and fibrosis models developed using different engineering methods such as organoids, microfluidic devices, and bioprinted constructs. We also present the benefits and limitations of emerging and promising regenerative therapy treatments for myocardial infarction such as cell therapies, extracellular vesicles, and cardiac patches. This review aims to overview recent advances in three-dimensional engineered infarct models and current regenerative therapeutic options, which can be used as a guide for developing new models and treatment strategies.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"3 3","pages":"031305"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9447372/pdf/BRIEIM-000003-031305_1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10166535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Single molecule DNA origami nanoarrays with controlled protein orientation. 具有可控蛋白质定向的单分子 DNA 折纸纳米阵列。

IF 2.9

Biophysics reviews Pub Date : 2022-08-18 eCollection Date: 2022-09-01 DOI: 10.1063/5.0099294

K Cervantes-Salguero, M Freeley, R E A Gwyther, D D Jones, J L Chávez, M Palma

{"title":"Single molecule DNA origami nanoarrays with controlled protein orientation.","authors":"K Cervantes-Salguero, M Freeley, R E A Gwyther, D D Jones, J L Chávez, M Palma","doi":"10.1063/5.0099294","DOIUrl":"10.1063/5.0099294","url":null,"abstract":"The nanoscale organization of functional (bio)molecules on solid substrates with nanoscale spatial resolution and single-molecule control-in both position and orientation-is of great interest for the development of next-generation (bio)molecular devices and assays. Herein, we report the fabrication of nanoarrays of individual proteins (and dyes) via the selective organization of DNA origami on nanopatterned surfaces and with controlled protein orientation. Nanoapertures in metal-coated glass substrates were patterned using focused ion beam lithography; 88% of the nanoapertures allowed immobilization of functionalized DNA origami structures. Photobleaching experiments of dye-functionalized DNA nanostructures indicated that 85% of the nanoapertures contain a single origami unit, with only 3% exhibiting double occupancy. Using a reprogrammed genetic code to engineer into a protein new chemistry to allow residue-specific linkage to an addressable ssDNA unit, we assembled orientation-controlled proteins functionalized to DNA origami structures; these were then organized in the arrays and exhibited single molecule traces. This strategy is of general applicability for the investigation of biomolecular events with single-molecule resolution in defined nanoarrays configurations and with orientational control of the (bio)molecule of interest.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"3 3","pages":"031401"},"PeriodicalIF":2.9,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10903486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140177974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating mechanism-based modeling with biomedical imaging to build practical digital twins for clinical oncology. 将基于机制的建模与生物医学成像相结合，为临床肿瘤学构建实用的数字孪生体。

Biophysics reviews Pub Date : 2022-06-01 Epub Date: 2022-05-17 DOI: 10.1063/5.0086789

Chengyue Wu, Guillermo Lorenzo, David A Hormuth, Ernesto A B F Lima, Kalina P Slavkova, Julie C DiCarlo, John Virostko, Caleb M Phillips, Debra Patt, Caroline Chung, Thomas E Yankeelov

{"title":"Integrating mechanism-based modeling with biomedical imaging to build practical digital twins for clinical oncology.","authors":"Chengyue Wu, Guillermo Lorenzo, David A Hormuth, Ernesto A B F Lima, Kalina P Slavkova, Julie C DiCarlo, John Virostko, Caleb M Phillips, Debra Patt, Caroline Chung, Thomas E Yankeelov","doi":"10.1063/5.0086789","DOIUrl":"10.1063/5.0086789","url":null,"abstract":"Digital twins employ mathematical and computational models to virtually represent a physical object (e.g., planes and human organs), predict the behavior of the object, and enable decision-making to optimize the future behavior of the object. While digital twins have been widely used in engineering for decades, their applications to oncology are only just emerging. Due to advances in experimental techniques quantitatively characterizing cancer, as well as advances in the mathematical and computational sciences, the notion of building and applying digital twins to understand tumor dynamics and personalize the care of cancer patients has been increasingly appreciated. In this review, we present the opportunities and challenges of applying digital twins in clinical oncology, with a particular focus on integrating medical imaging with mechanism-based, tissue-scale mathematical modeling. Specifically, we first introduce the general digital twin framework and then illustrate existing applications of image-guided digital twins in healthcare. Next, we detail both the imaging and modeling techniques that provide practical opportunities to build patient-specific digital twins for oncology. We then describe the current challenges and limitations in developing image-guided, mechanism-based digital twins for oncology along with potential solutions. We conclude by outlining five fundamental questions that can serve as a roadmap when designing and building a practical digital twin for oncology and attempt to provide answers for a specific application to brain cancer. We hope that this contribution provides motivation for the imaging science, oncology, and computational communities to develop practical digital twin technologies to improve the care of patients battling cancer.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"3 2","pages":"021304"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119003/pdf/BRIEIM-000003-021304_1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9490272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cataloguing the proteome: Current developments in single-molecule protein sequencing. 蛋白质组编目：单分子蛋白质测序的最新发展。

Biophysics reviews Pub Date : 2022-02-08 eCollection Date: 2022-03-01 DOI: 10.1063/5.0065509

Morgan M Brady, Anne S Meyer

引用次数: 0

Graphene nanostructures for input-output bioelectronics. 用于输入输出生物电子学的石墨烯纳米结构。

Biophysics reviews Pub Date : 2021-12-01 DOI: 10.1063/5.0073870

Raghav Garg, Daniel San Roman, Yingqiao Wang, Devora Cohen-Karni, Tzahi Cohen-Karni

{"title":"Graphene nanostructures for input-output bioelectronics.","authors":"Raghav Garg, Daniel San Roman, Yingqiao Wang, Devora Cohen-Karni, Tzahi Cohen-Karni","doi":"10.1063/5.0073870","DOIUrl":"https://doi.org/10.1063/5.0073870","url":null,"abstract":"The ability to manipulate the electrophysiology of electrically active cells and tissues has enabled a deeper understanding of healthy and diseased tissue states. This has primarily been achieved via input/output (I/O) bioelectronics that interface engineered materials with biological entities. Stable long-term application of conventional I/O bioelectronics advances as materials and processing techniques develop. Recent advancements have facilitated the development of graphene-based I/O bioelectronics with a wide variety of functional characteristics. Engineering the structural, physical, and chemical properties of graphene nanostructures and integration with modern microelectronics have enabled breakthrough high-density electrophysiological investigations. Here, we review recent advancements in 2D and 3D graphene-based I/O bioelectronics and highlight electrophysiological studies facilitated by these emerging platforms. Challenges and present potential breakthroughs that can be addressed via graphene bioelectronics are discussed. We emphasize the need for a multidisciplinary approach across materials science, micro-fabrication, and bioengineering to develop the next generation of I/O bioelectronics.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"2 4","pages":"041304"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717360/pdf/BRIEIM-000002-041304_1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10820587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Should biophysics study nonphysical quantities of biological systems? Take Max Delbrück for inspiration. 生物物理学应该研究生物系统的非物理量吗?以Max delbr<e:1> ck为例。

Biophysics reviews Pub Date : 2021-12-01 Epub Date: 2021-12-21 DOI: 10.1063/5.0079700

Sui Huang

引用次数: 0

Coupling between cardiac cells-An important determinant of electrical impulse propagation and arrhythmogenesis. 心脏细胞间的耦合——电脉冲传播和心律失常发生的重要决定因素。

Biophysics reviews Pub Date : 2021-09-01 Epub Date: 2021-07-13 DOI: 10.1063/5.0050192

André G Kléber, Qianru Jin

{"title":"Coupling between cardiac cells-An important determinant of electrical impulse propagation and arrhythmogenesis.","authors":"André G Kléber, Qianru Jin","doi":"10.1063/5.0050192","DOIUrl":"https://doi.org/10.1063/5.0050192","url":null,"abstract":"Cardiac arrhythmias are an important cause of sudden cardiac death-a devastating manifestation of many underlying causes, such as heart failure and ischemic heart disease leading to ventricular tachyarrhythmias and ventricular fibrillation, and atrial fibrillation causing cerebral embolism. Cardiac electrical propagation is a main factor in the initiation and maintenance of cardiac arrhythmias. In the heart, gap junctions are the basic unit at the cellular level that host intercellular low-resistance channels for the diffusion of ions and small regulatory molecules. The dual voltage clamp technique enabled the direct measurement of electrical conductance between cells and recording of single gap junction channel openings. The rapid turnover of gap junction channels at the intercalated disk implicates a highly dynamic process of trafficking and internalization of gap junction connexons. Recently, non-canonical roles of gap junction proteins have been discovered in mitochondria function, cytoskeletal organization, trafficking, and cardiac rescue. At the tissue level, we explain the concepts of linear propagation and safety factor based on the model of linear cellular structure. Working myocardium is adequately represented as a discontinuous cellular network characterized by cellular anisotropy and connective tissue heterogeneity. Electrical propagation in discontinuous cellular networks reflects an interplay of three main factors: cell-to-cell electrical coupling, flow of electrical charge through the ion channels, and the microscopic tissue structure. This review provides a state-of-the-art update of the cardiac gap junction channels and their role in cardiac electrical impulse propagation and highlights a combined approach of genetics, cell biology, and physics in modern cardiac electrophysiology.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"2 3","pages":"031301"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281002/pdf/BRIEIM-000002-031301_1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39211245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Forgotten but not gone: Particulate matter as contaminations of mucosal systems. 被遗忘但未消失：作为粘膜系统污染物的微粒物质。

Biophysics reviews Pub Date : 2021-08-10 eCollection Date: 2021-09-01 DOI: 10.1063/5.0054075

Matthias Marczynski, Oliver Lieleg

{"title":"Forgotten but not gone: Particulate matter as contaminations of mucosal systems.","authors":"Matthias Marczynski, Oliver Lieleg","doi":"10.1063/5.0054075","DOIUrl":"https://doi.org/10.1063/5.0054075","url":null,"abstract":"A decade ago, environmental issues, such as air pollution and the contamination of the oceans with microplastic, were prominently communicated in the media. However, these days, political topics, as well as the ongoing COVID-19 pandemic, have clearly taken over. In spite of this shift in focus regarding media representation, researchers have made progress in evaluating the possible health risks associated with particulate contaminations present in water and air. In this review article, we summarize recent efforts that establish a clear link between the increasing occurrence of certain pathological conditions and the exposure of humans (or animals) to airborne or waterborne particulate matter. First, we give an overview of the physiological functions mucus has to fulfill in humans and animals, and we discuss different sources of particulate matter. We then highlight parameters that govern particle toxicity and summarize our current knowledge of how an exposure to particulate matter can be related to dysfunctions of mucosal systems. Last, we outline how biophysical tools and methods can help researchers to obtain a better understanding of how particulate matter may affect human health. As we discuss here, recent research has made it quite clear that the structure and functions of those mucosal systems are sensitive toward particulate contaminations. Yet, our mechanistic understanding of how (and which) nano- and microparticles can compromise human health via interacting with mucosal barriers is far from complete.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"2 3","pages":"031302"},"PeriodicalIF":0.0,"publicationDate":"2021-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10903497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140177978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bringing the interaction of silver nanoparticles with bacteria to light. 揭示纳米银粒子与细菌的相互作用。

Biophysics reviews Pub Date : 2021-06-22 eCollection Date: 2021-06-01 DOI: 10.1063/5.0048725

Simone Normani, Nicholas Dalla Vedova, Guglielmo Lanzani, Francesco Scotognella, Giuseppe Maria Paternò

{"title":"Bringing the interaction of silver nanoparticles with bacteria to light.","authors":"Simone Normani, Nicholas Dalla Vedova, Guglielmo Lanzani, Francesco Scotognella, Giuseppe Maria Paternò","doi":"10.1063/5.0048725","DOIUrl":"https://doi.org/10.1063/5.0048725","url":null,"abstract":"In past decades, the exploitation of silver nanoparticles in novel antibacterial and detection devices has risen to prominence, owing to the well-known specific interaction of silver with bacteria. The vast majority of the investigations focus on the investigation over the mechanism of action underpinning bacterial eradication, while few efforts have been devoted to the study of the modification of silver optical properties upon interaction with bacteria. Specifically, given the characteristic localized surface plasmon resonance of silver nanostructures, which is sensitive to changes in the charge carrier density or in the dielectric environment, these systems can offer a handle in the detection of bacteria pathogens. In this review, we present the state of art of the research activity on the interaction of silver nanoparticles with bacteria, with strong emphasis on the modification of their optical properties. This may indeed lead to easy color reading of bacterial tests and pave the way to the development of nanotechnologic silver-based bacterial detection systems and drug-screening platforms.","PeriodicalId":72405,"journal":{"name":"Biophysics reviews","volume":"2 2","pages":"021304"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10903507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140177977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0