Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe最新文献

{"title":"Identification and quantification of ionising radiation-induced oxysterol formation in membranes of lens fibre cells","authors":"Alice Uwineza ,&nbsp;Ian Cummins ,&nbsp;Miguel Jarrin ,&nbsp;Alexia A. Kalligeraki ,&nbsp;Stephen Barnard ,&nbsp;Marco Mol ,&nbsp;Genny Degani ,&nbsp;Alessandra A. Altomare ,&nbsp;Giancarlo Aldini ,&nbsp;An Schreurs ,&nbsp;Detlef Balschun ,&nbsp;Elizabeth A. Ainsbury ,&nbsp;Irundika HK Dias ,&nbsp;Roy A. Quinlan","doi":"10.1016/j.arres.2022.100057","DOIUrl":"10.1016/j.arres.2022.100057","url":null,"abstract":"<div><p>Ionising radiation (IR) is a cause of lipid peroxidation, and epidemiological data have revealed a correlation between exposure to IR and the development of eye lens cataracts. Cataracts remain the leading cause of blindness around the world. The plasma membranes of lens fibre cells are one of the most cholesterolrich membranes in the human body, forming lipid rafts and contributing to the biophysical properties of lens fibre plasma membrane. Liquid chromatography followed by mass spectrometry was used to analyse bovine eye lens lipid membrane fractions after exposure to 5 and 50 Gy and eye lenses taken from wholebody 2 Gy-irradiated mice. Although cholesterol levels do not change significantly, IR dose-dependant formation of the oxysterols 7β-hydroxycholesterol, 7-ketocholesterol and 5, 6-epoxycholesterol in bovine lens nucleus membrane extracts was observed. Whole-body X-ray exposure (2 Gy) of 12-week old mice resulted in an increase in 7β-hydroxycholesterol and 7-ketocholesterol in their eye lenses. Their increase regressed over 24 h in the living lens cortex after IR exposure. This study also demonstrated that the IR-induced fold increase in oxysterols was greater in the mouse lens cortex than the nucleus. Further work is required to elucidate the mechanistic link(s) between oxysterols and IR-induced cataract, but these data evidence for the first time that IR exposure of mice results in oxysterol formation in their eye lenses.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"7 ","pages":"Article 100057"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49395198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The fundamental contribution of prof. Stirpe (and his research group) to the broadening of the scientific perspective on xanthine oxidoreductase","authors":"Maria Giulia Battelli,&nbsp;Massimo Bortolotti,&nbsp;Letizia Polito,&nbsp;Andrea Bolognesi","doi":"10.1016/j.arres.2022.100055","DOIUrl":"10.1016/j.arres.2022.100055","url":null,"abstract":"<div><p>Human xanthine oxidoreductase (XOR) is a multilevel regulated enzyme, which has many physiological functions, but which is also involved in several pathological processes. The contributions of Stirpe and his research group at the University of Bologna, to the development of knowledge on the XOR enzyme and its implications in physiological and pathological processes made a breakthrough in this field. Furthermore, their pioneering results paved the way for many research lines that are still actual and that have relevant clinical implications.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"7 ","pages":"Article 100055"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46976067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular interactions of resveratrol with Aβ 42 peptide and fibril during in-vitro Aβ 42 aggregation","authors":"Sheetal Sharma ,&nbsp;Hemant Goyal ,&nbsp;Shubhi Joshi ,&nbsp;Bimla Nehru ,&nbsp;Avneet Saini","doi":"10.1016/j.arres.2023.100060","DOIUrl":"10.1016/j.arres.2023.100060","url":null,"abstract":"<div><p>Amyloid aggregates are responsible for the development of oxidative stress and neurotoxicity in the brain. Several amino acid residues of amyloid beta (Aβ) 42 establish different molecular interactions to form and stabilize these aggregates, which can be targeted to prevent the aggregation. Resveratrol (Res) has inhibitory properties against Aβ 42 aggregation but studies elucidating its interactions with different residues of Aβ 42 aggregates are scarce. In the present study, we have discerned the molecular interactions of Res with different amino acid residues of Aβ 42 peptide and fibril during <em>in-vitro</em> Aβ 42 aggregation. Inhibitory properties of Res against amyloid aggregation were established through ANS and Thioflavin-T fluorescence assay, congo red assay and CD spectroscopy. The molecular interactions were established through molecular docking and 100 ns molecular dynamics simulations. The hydrophobic regions of Aβ 42 peptide, responsible for the formation of aggregates, were better protected in the presence of Res as indicated by ANS assay. Th-T and congo red assay suggested that Res prevented the formation of cross β-sheet structures. CD spectra analysis revealed that in the presence of Res, the secondary structure content was significantly decreased. MD simulation analysis revealed that Res formed strong molecular interactions with hydrophobic and secondary structure forming amino acid residues, which are involved in the amyloid aggregation and stabilization of aggregates. In conclusion, these interactions might have led to the decline in secondary structure content, formation of unordered nontoxic aggregates and prevention of the formation of sufuranyl free radical.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"7 ","pages":"Article 100060"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42600108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can systemic parenteral ozone therapy generate biological ozone? A new hypothesis","authors":"Maritza F. Díaz-Gómez ,&nbsp;Frank Hernández-Rosales","doi":"10.1016/j.arres.2023.100063","DOIUrl":"10.1016/j.arres.2023.100063","url":null,"abstract":"<div><p>Since the first years of this century, evidence of the generation of singlet oxygen and endogenous ozone from antibodies and some amino acids has been reported. Different mechanisms of action have been proposed which provide appropriate pathways that scientifically justify their approval. On the other hand, parenteral systemic ozone therapy is known to produce reactive oxygen species (ROS) that by chemical interconversion could form singlet oxygen, which is the precursor to trigger the formation of endogenous ozone. This article analyzes the latest evidences reported on the generation of biological ozone, and highlights the hypothesis that ROS resulting from the application of parenteral ozone therapy can also lead to the endogenous formation of ozone or an oxidant like ozone. Future confirmation of this approach would be another justification for the usefulness of ozone therapy in the treatment of diseases such as viral ones and antibiotic resistance.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"7 ","pages":"Article 100063"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47450669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strong Dual Antipolymerase/Antiexonuclease Actions of Some Aminothiadiazole Antioxidants: A Promising <i>In-Silico</i>/<i>In-Vitro</i> Repurposing Research Study against the COVID-19 Omicron Virus (B.1.1.529.3 Lineage).","authors":"Amgad M Rabie, Wafa A Eltayb","doi":"10.1016/j.arres.2023.100064","DOIUrl":"10.1016/j.arres.2023.100064","url":null,"abstract":"<p><p>Currently, nitrogen-containing heterocyclic virucides take the lead as top options for treating the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections and their escorting disease, the coronavirus disease 2019 (COVID-19). But unfortunately, the sudden emergence of a new strain of SARS-CoV-2, the Omicron variant and its lineages, complicated matters in the incessant COVID-19 battle. Goaling the two paramount coronaviral-2 multiplication enzymes RNA-dependent RNA polymerase (RdRp) and 3'-to-5' exoribonuclease (ExoN) at synchronous times using single ligand is a quite effective new binary avenue to restrain SARS-CoV-2 reproduction and cease COVID-19 progression irrespective of the SARS-CoV-2 strain type, as RdRps and ExoNs are vastly conserved in all SARS-CoV-2 strains. The presented <i>in-silico</i>/<i>in-vitro</i> research winnowed our own small libraries of antioxidant nitrogenous heterocyclic compounds, inspecting for the utmost convenient drug candidates expectedly capable of effectively working through this dual tactic. Computational screening afforded three promising compounds of the antioxidant 1,3,4-thiadiazole class, which were named ChloViD2022, Taroxaz-26, and CoViTris2022. Subsequent biological examination, employing the <i>in-vitro</i> anti-RdRp/anti-ExoN and anti-SARS-CoV-2 assays, exclusively demonstrated that ChloViD2022, CoViTris2022, and Taroxaz-26 could efficiently block the replication of the new lineages of SARS-CoV-2 with considerably minute anti-RdRp and anti-SARS-CoV-2 EC₅₀ values of about 0.18 and 0.44 μM for ChloViD2022, 0.22 and 0.72 μM for CoViTris2022, and 0.25 and 0.78 μM for Taroxaz-26, in the order, overtaking the standard anti-SARS-CoV-2 drug molnupiravir. These biochemical findings were optimally presupported by the results of the prior <i>in-silico</i> screening, suggesting that the three compounds might potently hit the catalytic active sites of the virus's RdRp and ExoN enzymes. Furthermore, the perfect pharmacophoric features of ChloViD2022, Taroxaz-26, and CoViTris2022 molecules make them typical dual inhibitors of SARS-CoV-2 replication and proofreading, with their relatively flexible structures eligible for diverse forms of chemical modification. In sum, the current important results of this thorough research work exposed the interesting repurposing potential of the three 2-amino-1,3,4-thiadiazole ligands, ChloViD2022, Taroxaz-26, and CoViTris2022, to effectively conflict with the vital biointeractions between the coronavirus's polymerase/exoribonuclease and the four essential RNA nucleotides, and, accordingly, arrest COVID-19 disease, persuading the relevant investigators to quickly begin the three agents' comprehensive preclinical and clinical anti-COVID-19 assessments.</p>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":" ","pages":"100064"},"PeriodicalIF":0.0,"publicationDate":"2023-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9907022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9185391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico targeting of lipoxygenase, CYP2C9, and NAD(P)H oxidase by major green tea polyphenols to subvert OS","authors":"Prem Rajak, Abhratanu Ganguly, M. Mandi, Anik Dutta, Saurabh Sarkar, Sayantani Nanda, K. Das, Siddhartha Ghanty, G. Biswas","doi":"10.1016/j.arres.2023.100061","DOIUrl":"https://doi.org/10.1016/j.arres.2023.100061","url":null,"abstract":"","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44124631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endogenous ozone as a regular reactive oxygen species in (patho) physiology","authors":"Arnold N. Onyango","doi":"10.1016/j.arres.2023.100075","DOIUrl":"https://doi.org/10.1016/j.arres.2023.100075","url":null,"abstract":"","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"9 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49776018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations in thiol redox state and lipid peroxidation in the brain areas of male mice during aging","authors":"Konstantinos Grintzalis ,&nbsp;Nikolaos Patsoukis ,&nbsp;Ioannis Papapostolou ,&nbsp;George Zervoudakis ,&nbsp;Electra Kalaitzopoulou ,&nbsp;Christos D. Georgiou ,&nbsp;Nikolaos A. Matsokis ,&nbsp;Nikolaos T. Panagopoulos","doi":"10.1016/j.arres.2022.100043","DOIUrl":"10.1016/j.arres.2022.100043","url":null,"abstract":"<div><p>Aging is a natural process in organisms with its underlying mechanisms remaining unknown. Brain aging is accompanied by cognitive deficits and movement disorders, which signify the importance of elaborating its main mechanisms. In this study, oxidative stress biomarkers for lipid peroxidation and thiol redox state were assessed in different brain areas in male mice, categorised in respect to aging as young (three months), middle (eleven months) and elder aged (twenty-three months). Senescence was associated with an increase of lipid peroxidation and a decrease of reduced and oxidized glutathione. In some brain areas, reduced cysteine and oxidized protein thiols were increased with aging. Results support the theory that aging is associated with oxidative stress in the brain of mice and provide an insight in the biochemical aspect of aging in reference to thiol redox status as a potential marker for aging.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"6 ","pages":"Article 100043"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667137922000157/pdfft?md5=5a6e5c0a963ad4754a239e0cf344c58e&pid=1-s2.0-S2667137922000157-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54184921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term selection for faster development and early reproduction leads to up-regulation of genes involved in redox homeostasis","authors":"Nidhi Krishna Shrivastava,&nbsp;Abhishek Kumar Farand,&nbsp;Mallikarjun N. Shakarad","doi":"10.1016/j.arres.2022.100045","DOIUrl":"10.1016/j.arres.2022.100045","url":null,"abstract":"<div><p>ROS produced by Duox is necessary for normal host survival in response to commensal and or infectious microbes. However, failure in the homeostatic balance between synthesis and elimination of ROS leads to damage of major macromolecules and eventual death of the organism. The \"evolutionary option\" to use ROS to perform biological functions, in particular, is a double-edged sword. Rapid development is suggested to result in increased production of ROS due to increased metabolic demands as a consequence of sustained proliferation. We assessed redox homeostasis by measuring the transcript levels of genes involved in ROS production (<em>Duox</em> and <em>Nox</em>) and scavenging (<em>Irc, Sod1</em> and <em>Cat</em>) in three population types of <em>Drosophila melanogaster.</em> We discuss the role and interplay between ROS generating and ROS scavenging genes in maintaining developmental integrity and physiological homeostasis under rapid development.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"6 ","pages":"Article 100045"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667137922000170/pdfft?md5=a7ccdd70351a20ab8d7e1d81a3f0a201&pid=1-s2.0-S2667137922000170-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46504966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diisononyl phthalate inhibits cardiac glycolysis and oxidative phosphorylation by down-regulating cytosolic and mitochondrial energy metabolizing enzymes in murine model","authors":"S. A Kehinde ,&nbsp;A. Ore ,&nbsp;A. T Olajide ,&nbsp;I. E Ajagunna ,&nbsp;F. A Oloyede ,&nbsp;T. O Faniyi ,&nbsp;J. O Fatoki","doi":"10.1016/j.arres.2022.100041","DOIUrl":"10.1016/j.arres.2022.100041","url":null,"abstract":"<div><p>The recent increase of diisononyl phthalate (DiNP) applications in a wide range of plastic consumer products, as well as its relative environmental exposure and interactions, has compelled an investigation of its toxicity. Phthalate exposure has been associated with heart dysfunction in animals in a few studies, the bulk of which are linked to the high molecular weight phthalates. The effect of DiNP on cardiac energy transduction was assessed utilizing cellular respiration enzymes as indices. Eighteen wistar rats were divided into 3 groups of six rats each: Group A received Tween-80 (control), DiNP (20 mg/kg/BW) was given to Group B, and 200mg/kg DiNP was given to Group C orally (gavage) for 14 days. The activity of cardiac glycolytic, oxidative phosphorylation enzymes and histopathological changes were assessed. The glycolytic and tricarboxylic acid cycle enzymes studied were predominantly down-regulated in a dose-dependent manner, with the exception of cardiac citrate synthase, which showed no significant variation in activity when compared to the control. Furthermore, all respiratory chain complexes (Complex I-IV) decreased significantly relative to control, with the exception of complex IV activity at 20mg/kg/BW which showed no significant difference <em>(P&lt;0.05)</em>. Cardiac histopathological alterations confirmed the aforementioned metabolic disturbances. Finally, DiNP exposure impairs cardiac energy transduction enzymes, implying cardiac cells produced insufficient energy (ATP) to carry out its morphological and physiological functions efficiently as the heart requires a constant supply of energy in the form of ATP to support contraction, relaxation, and prevent cardiomyopathies.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"6 ","pages":"Article 100041"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667137922000133/pdfft?md5=83080e2b30576955f45c4486d88b5dc8&pid=1-s2.0-S2667137922000133-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45413984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}