Acta Scientiarum. Biological Sciences最新文献_第8页

Cell culture and variations in the comet assay do not affect the genomic integrity of adipose-derived stem cells 细胞培养和彗星试验中的变异不影响脂肪来源干细胞的基因组完整性

Acta Scientiarum. Biological Sciences Pub Date : 2022-07-28 DOI: 10.4025/actascibiolsci.v44i1.60714

L. Bernardi, Laynna de Carvalho Schweich-Adami, Edwin José Torres de Oliveira, A. Baranoski, R. Oliveira, Andreia Conceição Milan Brochado Antoniolli da Silva

{"title":"Cell culture and variations in the comet assay do not affect the genomic integrity of adipose-derived stem cells","authors":"L. Bernardi, Laynna de Carvalho Schweich-Adami, Edwin José Torres de Oliveira, A. Baranoski, R. Oliveira, Andreia Conceição Milan Brochado Antoniolli da Silva","doi":"10.4025/actascibiolsci.v44i1.60714","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.60714","url":null,"abstract":"The application of quality control tests, such as the comet assay, are essential when adipose-derived stem cells are cultured for therapeutic purposes. However, the steps involved in the development of this assay should be investigated, in order to reduce their influence on genomic damage in cells. The study aimed to evaluate if the cell culture process causes DNA damage, and if variations in the lysis time and pH of the electrophoresis buffer interfere in the genotoxicity results. Four different comet assay protocols were evaluated, and the effects of lysis time and pH conditions of the electrophoresis buffer solution were stated as follows: 2 hours and pH 12; 24 hours and pH 12; 2 hours and pH ≥ 13 and 24 hours and pH ≥ 13. The tail moment was analyzed, and results indicated that at the time cells were detached from the flasks, there was little damage to the DNA in the adipose-derived stem cells, which was confirmed by evaluation of the expression of mRNA genes involved in damage and repair processes of genetic material. Also, the tail moment values did not show significant differences among the four evaluated protocols (p < 0.05), with no indication of damage when compared to the positive control (p < 0.05). Thus, any of the tested protocols can be applied in genotoxicity tests with adipose-derived stem cells, without causing damage to them.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86659855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Avifauna of the Serra de Itabaiana National Park revisited: additions and deletions in a period of 15 years 重新参观了Serra de Itabaiana国家公园的鸟类:在15年的时间里增加和删除

Acta Scientiarum. Biological Sciences Pub Date : 2022-07-28 DOI: 10.4025/actascibiolsci.v44i1.59923

C. Silva, Juan Ruiz-Esparza, C. Azevedo, Adauto de Souza Ribeiro

{"title":"Avifauna of the Serra de Itabaiana National Park revisited: additions and deletions in a period of 15 years","authors":"C. Silva, Juan Ruiz-Esparza, C. Azevedo, Adauto de Souza Ribeiro","doi":"10.4025/actascibiolsci.v44i1.59923","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.59923","url":null,"abstract":"The Serra de Itabaiana National Park (PARNASI) is known as an important conservation area in the ecotone between the Atlantic Forest and Caatinga in Northeast Brazil and is one of the main areas of fauna and flora studies in Sergipe. For this reason, there are some studies on its avifauna, which date back to its creation in 2005. However, after 15 years of establishment of PARNASI, an update of the inventory of birds is necessary due to adjustments in the management of the park. The present study aimed to update the bird inventory of the Serra de Itabaiana National Park, chronologically comparing the new ornithological records obtained, aiming to contribute to the knowledge and conservation of Sergipe's avifauna. The data were compiled from scientific publications, personal files and from documented records on a specialized website. The resulting list includes 227 bird species belonging to 49 families, including Thraupidae (28 species; 12.4%), Tyrannidae (25 species; 11.1%) and Accipitridae (17 species; 7.5%) as the most representative. The most representative guilds were insectivorous (87 species; 38.3%) and omnivorous (60 species; 26.4%), and by species independent of the forest environment (87 species; 38.3%). Fourteen bird species are threatened with extinction. Nine species are endemic to the Caatinga biome, eight to the Atlantic Forest and one to the Cerrado. We added 104 species to the first checklist produced for the PARNASI created in 2005, including Nyctibius grandis (Nyctibiidae), a new record for the state of Sergipe. PARNASI's updated list of bird species allows for a better assessment of its effectiveness in the conservation of the local avifauna, facilitating management actions that allow the coexistence and perpetuation of species over time.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89972649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Seed germination of Hesperozygis ringens (Benth.) Epling, an endemic and threatened species 桃皮草种子萌发的研究一种特有的濒危物种

Acta Scientiarum. Biological Sciences Pub Date : 2022-07-27 DOI: 10.4025/actascibiolsci.v44i1.58431

Leandro Gonçalves Leite, Raquel Stefanello, L. Essi

{"title":"Seed germination of Hesperozygis ringens (Benth.) Epling, an endemic and threatened species","authors":"Leandro Gonçalves Leite, Raquel Stefanello, L. Essi","doi":"10.4025/actascibiolsci.v44i1.58431","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.58431","url":null,"abstract":"Hesperozygis ringens (Lamiaceae), popularly known as espanta-pulga, is a threatened species endemic to rocky and sandy regions of the Pampa biome. One factor that can influence the low number of individuals of a species is a low seed germination rate influenced by temperature and/or light. Thus, the objective of this study was to evaluate the influence of light and temperature on the seed germination of H. ringens. The seeds of two lots were sown on a paper substrate and maintained in BOD chambers at temperatures of 15, 20, 25 and 30ºC in the presence and absence of light. The germination speed rate was evaluated every 3 days for 21 days. The experiment was completely randomized with treatments that had a 4 x 2 factorial design. The first visible sign (protrusion of the primary root) of germination was observed seven days after sowing. Germination occurred both in the presence and absence of light and the lowest temperatures significantly influenced the germination process and germination speed. For germinating the species, 15°C was the most favorable temperature compared to 20, 25 and 30°C. It can be concluded that a temperature of 15ºC favors the germination process of H. ringens seeds, which are insensitive to light.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89343147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breeding ecology of the Bare-Faced Ibis (Phimosus infuscatus) in Southern Brazil 巴西南部赤面朱鹮的繁殖生态学

Acta Scientiarum. Biological Sciences Pub Date : 2022-07-27 DOI: 10.4025/actascibiolsci.v44i1.58697

A. Ferreira, D. C. Melo, J. Branco

{"title":"Breeding ecology of the Bare-Faced Ibis (Phimosus infuscatus) in Southern Brazil","authors":"A. Ferreira, D. C. Melo, J. Branco","doi":"10.4025/actascibiolsci.v44i1.58697","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.58697","url":null,"abstract":"The Bare-faced Ibis is a gregarious bird found in South America. In the face of a rapid expansion in a southern state in Brazil and a lack of detailed information about the species' reproduction, two breeding areas were studied weekly for two consecutive seasons. We registered numbers and characteristics of adults, nests, eggs, and chicks, calculated success estimates, and assessed nest installation preferences. Chick morphology, age, and measurements served to define five growth stages. The species used the margins of two close rivers in Santa Catarina to build nests, lay eggs, and develop parental care between August and January, with success estimates as expected for the family. We observed a preference for the eastern margin of the rivers and proximity to bridges for nest installation, where the muddy margin provides resources for juvenile feeding. Two colony models were defined based on the near or far installation of the nests. Variations in egg and chick dimensions indicated higher adult investment in the second season, with negative allometric growth of the bill and tarsus of chicks related to weight. The results presented suggest favorable conditions for the species’ reproduction in the region, a considerable factor in understanding its expansion in recent years.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80202834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foraging Oxaea flavescens bees as a function of the dynamics of abiotic factors and food resource availability from Styrax camporum flowers 非生物因子和食物资源可得性对黄草蜜蜂觅食的影响

Acta Scientiarum. Biological Sciences Pub Date : 2022-07-27 DOI: 10.4025/actascibiolsci.v44i1.58568

L. P. Polatto, V. V. Alves Junior

{"title":"Foraging Oxaea flavescens bees as a function of the dynamics of abiotic factors and food resource availability from Styrax camporum flowers","authors":"L. P. Polatto, V. V. Alves Junior","doi":"10.4025/actascibiolsci.v44i1.58568","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.58568","url":null,"abstract":"We examined the abiotic factors and co-specific competition for food resources that regulate the foraging activity of Oxaea flavescens bees on Styrax camporum flowers. Foraging records were gathered during 20 min. periods at the beginning of each hour between 05:00h and 18:00h during three nonconsecutive days. Pearson correlation and linear regression tests indicated that the foraging activity of O. flavescens was associated with abiotic factors during the day. O. flavescens represented 89.9% of the observed foraging visits to S. camporum flowers. On the first day of sampling, when environmental conditions were stressful, the foraging activity of O. flavescens was significantly negatively correlated with light intensity, wind speed, and temperature, and positively correlated with relative humidity. Under those conditions, optimal foraging was little affected by the availability of floral resources. On the second and third days, however, when environmental conditions were more favorable, the principal limiting factor of O. flavescens foraging activity was nectar depletion. The maximum peak of foraging under those conditions occurred before the abiotic conditions were fully favorable, however, as the eventual depletion of floral resources resulted in unfavorable cost/benefit implications for foraging during the otherwise most adequate daylight period.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90665626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Color polymorphism and variation in microhabitat selection: a defensive strategy in males of Boana pulchella (Anura: Hylidae)? 色型多态性和微生境选择的变异:一种雄性白眉猴的防御策略

Acta Scientiarum. Biological Sciences Pub Date : 2022-07-27 DOI: 10.4025/actascibiolsci.v44i1.58763

L. N. Lermen, Raíssa Furtado, S. Hartz

{"title":"Color polymorphism and variation in microhabitat selection: a defensive strategy in males of Boana pulchella (Anura: Hylidae)?","authors":"L. N. Lermen, Raíssa Furtado, S. Hartz","doi":"10.4025/actascibiolsci.v44i1.58763","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.58763","url":null,"abstract":"Anurans vary in body coloration and frequently exhibit color polymorphism, with selection by predation pressure favoring more cryptic animals. Spatial differentiation may also favor color polymorphism in some species, since cryptic morphotypes can use substrates that better match their coloration. Boana pulchella is a polymorphic species with green and brown morphotypes, but its polymorphism remains poorly understood. The aim of the present study was to determine the functionality of color polymorphism in B. pulchella as a defense strategy. Artificial green and brown clay models were placed in environments with green and brown background to determine if the two morphotypes of B. pulchella experience different frequencies of attacks. We expected that more conspicuous morphs are attacked more frequently. The attack rate, which suggests predation intent, on the models was 9.57%. The marks observed in the artificial models were left by both mammals and birds. The number of damaged models did not significantly differ between the different background environments. The results indicate that B. pulchella morphotypes are equally cryptic to predators in the studied environments. In conclusion, probably color polymorphism in B. pulchella is not operating as a defensive strategy, and other selective forces may be acting in the maintenance of color polymorphism in this species.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"08 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86500337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient, fast and low-cost strategies for DNA extraction from different nucleated sheep cells 高效、快速、低成本提取不同有核绵羊细胞DNA的方法

Acta Scientiarum. Biological Sciences Pub Date : 2022-07-27 DOI: 10.4025/actascibiolsci.v44i1.59272

A. Santos, Wilson Romão Toledo da Silva, H. N. Ferreira, Antonio Matos Fraga Junior, M. Batista, J. Velarde

{"title":"Efficient, fast and low-cost strategies for DNA extraction from different nucleated sheep cells","authors":"A. Santos, Wilson Romão Toledo da Silva, H. N. Ferreira, Antonio Matos Fraga Junior, M. Batista, J. Velarde","doi":"10.4025/actascibiolsci.v44i1.59272","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.59272","url":null,"abstract":"DNA extraction is usually the first step to perform molecular studies. This process can be nonviable due to genomic DNA (gDNA) extraction commercial kits prices. Furthermore, available DNA extraction protocols generally have high specificity, limiting their use to specific sources of biological material. In order to reduce costs, optimize time and laboratory logistics, besides to demonstrate a versatile protocol, the present study worked on an efficient DNA extraction protocol from somatic and non-somatic cells, using biological material from sheep as a model. For that, gDNA was extracted from whole blood, spermatozoa, and hair bulb cells, collected from three adult sheep, transported at 5ºC and stored at -20ºC until lab procedures. After extraction, gDNA concentration and purity were evaluated in a nano spectrophotometer. gDNA concentration from whole blood was greater (p < 0.05) than extracted from hair bulb cells, which in turn was superior (p < 0.05) than in spermatozoa. Also, gDNA from whole blood and, followed by, sperm showed greater (p < 0.05) purity when compared to gDNA of hair bulb cells. Adapting a gDNA extraction protocol, originally developed for bovine whole blood, enabled to obtain and isolate gDNA in different nucleated sheep cells","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72516333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Socio-environmental assessment of the impact of the African snail, Lissachatina fulica (Férussac), 1821, on the population of Alto Paraíso de Goiás, Brazil 1821年非洲蜗牛Lissachatina fulica (fsamurussac)对巴西Alto Paraíso de Goiás人口影响的社会环境评估

Acta Scientiarum. Biological Sciences Pub Date : 2022-06-01 DOI: 10.4025/actascibiolsci.v44i1.59212

Lígia Cristina Cazarin-Oliveira, L. P. Fraga, Manoela Volkweis Lombardi, Fabiana Sperb Volkweis, Maria Júlia Martins Silva

{"title":"Socio-environmental assessment of the impact of the African snail, Lissachatina fulica (Férussac), 1821, on the population of Alto Paraíso de Goiás, Brazil","authors":"Lígia Cristina Cazarin-Oliveira, L. P. Fraga, Manoela Volkweis Lombardi, Fabiana Sperb Volkweis, Maria Júlia Martins Silva","doi":"10.4025/actascibiolsci.v44i1.59212","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.59212","url":null,"abstract":"The introduction of the African snail Lissachatina fulica worldwide poses a risk to the environment, agriculture and human health. Considered a socio-environmental pest it causes problems to plantations and gardens, in addition to threatening native species. Also, it can be an intermediate host of worms that affect humans and mammals. This work aims to evaluate the socioenvironmental impact caused by L. fulica in Alto Paraíso de Goiás, Goiás, Brazil. Specifically, it aimed to analyze the residents' knowledge about L. fulica, if they were able to recognize the invasive snail and differentiate it from the giant native snail Megalobulimus sp., in addition to the community's ability to carry out actions to combat the invasive species. Semi-structured interviews were conducted, guided by a questionnaire, with the urban population, and an unstructured interview with a health surveillance agent in the municipality. The area of the city was divided into five zones and ten residences were visited in each zone. It was found that the residents most affected by L. fulica are those who live in the most urbanized region of the city, with 86% of respondents reporting seeing the animal in the city center. The presence of the Megalobulimus sp. was reported by four residents in the city's border regions with the native Cerrado. Most of the reports of the African snail in the houses occurred by residents of Alto Paraíso de Goiás who had plants in their backyard and personally took care of them.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75257436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diet of three juvenile fish species of the coastal zone of a Southwest Atlantic beach, Brazil 巴西西南大西洋海滩海岸带三种幼鱼的饮食

Acta Scientiarum. Biological Sciences Pub Date : 2022-05-18 DOI: 10.4025/actascibiolsci.v44i1.53490

Jessica Knoeller, A. Tomás, T. Vaske Júnior

{"title":"Diet of three juvenile fish species of the coastal zone of a Southwest Atlantic beach, Brazil","authors":"Jessica Knoeller, A. Tomás, T. Vaske Júnior","doi":"10.4025/actascibiolsci.v44i1.53490","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.53490","url":null,"abstract":"Surf zones are associated with a shelter area, nursery and feeding for several species of fish. In this study, the dietary habits of three species of fish with demersal habits (Gulf kingcroaker Menticirrhu gracilis, Whitesea catfish Genidens barbus and Barbu Polydacytlus virginicus), were verified in the surf zone area of an active urban beach of Baixada Santista, concerning structure in size, day and night abundances and overlapping diets. A total of 617 fish were analyzed, of which 362 were 194 were G. barbus and 61 were P. virginicus. Stomach contents, represented in Relative Importance Index diagrams, showed that the bivalve Donax gemmula is the most important food item for M. gracilis, scales of Teleostei for G. barbus and Euphausiacea for P. virginicus. In order to evaluate the similarity of the diets, the Macarthur & Levins indices and a cluster analysis with the Bray-Curtis distance were applied revealing that the diet is 44% different between G. barbus and M. gracilis, 5% between G. barbus and P. virginicus and of 84% between M. gracilis and P. virginicus. Anthropogenic waste like plastic, nylon, metal pieces, were only occasionaly observed.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73598778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan and chitin production and extraction in isolates of Cunninghamella sp. Cunninghamella sp.分离株壳聚糖和几丁质的制备及提取。

Acta Scientiarum. Biological Sciences Pub Date : 2022-05-18 DOI: 10.4025/actascibiolsci.v44i1.59982

A. F. Chagas Júnior, L. F. B. Chagas, G. N. Scheidt, V. Chapla, B. S. O. Colonia, M. Souza, Albert Lennon Lima Martins

{"title":"Chitosan and chitin production and extraction in isolates of Cunninghamella sp.","authors":"A. F. Chagas Júnior, L. F. B. Chagas, G. N. Scheidt, V. Chapla, B. S. O. Colonia, M. Souza, Albert Lennon Lima Martins","doi":"10.4025/actascibiolsci.v44i1.59982","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.59982","url":null,"abstract":"The use of fungi to obtain chitin and chitosan has advantages such as handling, extraction, and production control, thus allowing to generate high quality chitosan. This study aimed to isolate and identify strains of Cunninghamella spp., and assess the production of biomass, chitin, and chitosan. We determined the macroscopic and microscopic phenotypic aspects of the superiors, mycelial growth rate index, consumption of glucose and nitrogen, as well as the pH variation, and the production of biomass, chitin, and chitosan. After assessing the mycelial growth speed index, considered fast, we found that the UFT-Ce08 and UFT-Ce09 isolates showed a higher growth speed. Therefore, we assessed the sporulation of the isolates, and all of them reached the concentration of 106 spores mL-1 in the period of 96 hours. The YPD was considered ideal for biomass production because it promotes an efficient consumption and synthesis of organic compounds by microorganisms. The assessment of the biomass production and the chitin and chitosan yield of nine isolates and the reference strain showed that the UFT-Ce08 isolate had the highest amount of biomass, the UFT-Ce11 isolate had the highest chitin yield, and despite having the second smallest biomass, the UFT-Ce09 isolate had the highest chitosan yield. Seven isolates showed higher chitosan yield than the reference strain. Therefore, chitosan production from this fungus can be further optimized to improve yield on a large-scale production. Chitosan production from this fungus can be further optimized to improve yield in large-scale production","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89477378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0