Acta Scientiarum. Biological Sciences最新文献_第6页

Microalgal production under mixotrophic conditions using cheese whey as substrate 混合营养条件下以奶酪乳清为底物的微藻生产

Acta Scientiarum. Biological Sciences Pub Date : 2022-10-28 DOI: 10.4025/actascibiolsci.v44i1.62512

A. Andrade, P. E. C. E. Silva, Rebeca Gonçalves de Melo, Millena Patrício do Nascimento Ferreira, A. Porto, R. Bezerra

{"title":"Microalgal production under mixotrophic conditions using cheese whey as substrate","authors":"A. Andrade, P. E. C. E. Silva, Rebeca Gonçalves de Melo, Millena Patrício do Nascimento Ferreira, A. Porto, R. Bezerra","doi":"10.4025/actascibiolsci.v44i1.62512","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.62512","url":null,"abstract":"Microalgae are known for producing various biotechnological products. Moreover, they absorb nutrients from dairy wastewater, grow well, and accumulate valuable compounds faster. In this study, photoautotrophic and mixotrophic cultivation with different initial lactose concentrations present in cheese whey (CW) were established to investigate their effect on cell concentration (Xm, mg L-1), cell productivity (Px, mg L-1day-1), and specific cell growth (µmax, day-1) of Chlorella vulgaris, Dunaliella tertiolecta, and Tetradesmus obliquus. The biomass production of C. vulgaris (Xm= 1,520 ± 30.3 mg L-1, Px = 147 ± 3.00 mg L-1, and µmax = 0.150 ± 0.00 mg L-1) in mixotrophic culture with 10.0 g L-1 of lactose, the main constituent of CW, was notably enhanced by 55% in comparison with their photoautotrophic cultures, whereas a lower effect of these lactose concentrations on cell growth was observed in T. obliquus and D. tertiolecta. Thus, mixotrophic cultivation of C. vulgaris using CW as a carbon and energy source could be considered a feasible alternative to obtain high value-added biomass.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74016537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of leaf quality and colonization time on the abundance of bacteria in an experimental design 叶片品质和定殖时间对细菌丰度的影响

Acta Scientiarum. Biological Sciences Pub Date : 2022-10-28 DOI: 10.4025/actascibiolsci.v44i1.61495

Vinícius da Silva Rasvailer, Camila Gentilin-Avanci, M. Scoarize, G. D. Pinha, E. Benedito

{"title":"Effects of leaf quality and colonization time on the abundance of bacteria in an experimental design","authors":"Vinícius da Silva Rasvailer, Camila Gentilin-Avanci, M. Scoarize, G. D. Pinha, E. Benedito","doi":"10.4025/actascibiolsci.v44i1.61495","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.61495","url":null,"abstract":"Small rivers, henceforth streams, depend on organic matter (nutrients and energy) from riparian vegetation. The quality of such allochthonous debris is determinant for the transformation of organic matter compounds, where the bacterial community has a crucial role in the final decomposition of the substrate. During bacterial colonization, debris with higher concentration of nutrients (more palatable) is prioritized, which accelerates the process. This study investigated the effects of leaf palatability of two native trees on bacterial colonization (abundance) over time, through a laboratory experiment that lasted 60 days. Values of C, N, P, C:N, C:P, polyphenols, tannins, lignin, lignin:N and leaf toughness of both species were compared. Bacterial abundance was higher in species with higher nitrogen values, although they had higher leaf toughness and more polyphenols, which differs from studies indicating that high leaf toughness represents low nutritional quality. The colonization time did not influence bacterial abundance. Therefore, processes degrading riparian vegetation and reducing nutritional quality can affect local decomposition, decreasing bacterial abundance.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90676318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a facial biocosmetic containing levan, almond and cinnamon oils with antioxidant and moisturizing properties 开发一种面部生物化妆品，含有具有抗氧化和保湿特性的levan，杏仁和肉桂油

Acta Scientiarum. Biological Sciences Pub Date : 2022-10-28 DOI: 10.4025/actascibiolsci.v44i1.58869

R. Silva, Gabrielly Terassi Bersaneti, B. G. Bigotto, V. A. I. Silveira, A. Lonni, D. Borsato, M. Celligoi

{"title":"Development of a facial biocosmetic containing levan, almond and cinnamon oils with antioxidant and moisturizing properties","authors":"R. Silva, Gabrielly Terassi Bersaneti, B. G. Bigotto, V. A. I. Silveira, A. Lonni, D. Borsato, M. Celligoi","doi":"10.4025/actascibiolsci.v44i1.58869","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.58869","url":null,"abstract":"The addition of natural molecules such as microbial exopolysaccharides in cosmetics is a trend in the current market, adding properties and improving the product quality. Therefore, the aim of this study was to develop a facial biocosmetic formulation containing microbial levan, almond and cinnamon oils. The centroid-simplex design was used to evaluate the spreadability, antioxidant activity, moisture retention capacity and viscosity of formulations. Since it is a facial cosmetic, the formulation was optimized using the intermediate viscosity. The optimized formulation with intermediate viscosity was 75% (0.75 g) levan and 25% (2 mL) almond oil, without the addition of cinnamon oil. This formulation was submitted to 90 days under different exposure conditions, and the results showed a spreadability of 805 mm2, pH and density ideal for the facial area, with an antioxidant activity of 72%, hydration capacity of 100.3%, viscosity with no-Newtonian behavior, and normal organoleptic properties when stored at room and low temperature. The formulation with levan associated with almond oil showed potential for application in the facial area, with high antioxidant properties, moisturizing intermediate viscosity and stability for 90 days. The utilization of centroid-simplex design allowed the development of a biocosmetic with desired characteristics just by adjusting the concentrations of the bioactive.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87223143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of the food matrix on dual-species biofilm of Listeria monocytogenes and Pseudomonas fluorescens 食物基质对单核增生李斯特菌和荧光假单胞菌双种生物膜的影响

Acta Scientiarum. Biological Sciences Pub Date : 2022-10-28 DOI: 10.4025/actascibiolsci.v44i1.61925

Daniel Leirias Caurio, Danieli Quadros da Silva, L. Dornelles, Nathasha Noronha Arechavaleta, A. S. Mota

{"title":"Influence of the food matrix on dual-species biofilm of Listeria monocytogenes and Pseudomonas fluorescens","authors":"Daniel Leirias Caurio, Danieli Quadros da Silva, L. Dornelles, Nathasha Noronha Arechavaleta, A. S. Mota","doi":"10.4025/actascibiolsci.v44i1.61925","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.61925","url":null,"abstract":"In the food industry, the formation of biofilms results in serious microbial recontamination problem. This work aimed to study Listeria sp. obtained from sliced products and food handling surfaces, for the preparation of food of animal origin to be consumed cold. Listeria monocytogenes, Listeria innocua and Listeria seeligeri were detected. In the evaluation of antibiotic susceptibility, the isolates were sensitive to ampicillin and penicillin, which were the main antibiotics used in treatment. The isolates showed the ability to form biofilm in microplate, in stainless steel and polypropylene surfaces, with a variation of sessile cells between 4.29±0.23 and 7.03±0.01 log10 CFU cm-2 in TSBYE. This ability was also observed in food matrix composed of UHT whole milk in mono-species cultivation and in associated cultivation of L. monocytogenes with Pseudomonas fluorescens. The application of sanitizers peracetic acid and sodium hypochlorite revealed efficiency in the eradication of adhered cells and biofilms formed in stainless steel surfaces. Therefore, Listeria sp. showed to be persistent and able to form biofilm in mono-species cultivation or associated with P. fluorescens, under different conditions. Taking these aspects into account, the need for proper hygiene in the food production process is highlighted in order to avoid risks to the consumers health","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90423607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of aqueous and ethanolic extracts of Lepidium meyenii Walp (red and black maca) on Caenorhabditis elegans 红玛咖和黑玛咖水提液和醇提液对秀丽隐杆线虫的影响

Acta Scientiarum. Biological Sciences Pub Date : 2022-10-28 DOI: 10.4025/actascibiolsci.v44i1.58300

Saray Paternina-Ricardo, Barbara Arroyo Salgado, Maria Cecilia García Espiñeira

{"title":"Effects of aqueous and ethanolic extracts of Lepidium meyenii Walp (red and black maca) on Caenorhabditis elegans","authors":"Saray Paternina-Ricardo, Barbara Arroyo Salgado, Maria Cecilia García Espiñeira","doi":"10.4025/actascibiolsci.v44i1.58300","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.58300","url":null,"abstract":"Lepidium meyenii (maca) is a Peruvian nutraceutical plant, whose hypocotyl has a variety of colors ranging from black to white. The black and red varieties of maca have been the most studied since their extracts are associated with effects such as increased sperm count, decreased glucose levels, reversal of prostatic hyperplasia, among others. However, the properties related to reduction of oxidative stress, metabolic diseases and anti-aging have not yet been confirmed. The aim was to evaluate the effects of aqueous and ethanolic extracts, obtained from spray-dried hydroalcoholic extract of hypocotyl of black and red maca (Lepidium meyenii), on mortality, growth, reproduction, lipid accumulation and the expression levels of genes related to oxidative stress and heat shock, in the in-vivo model, Caenorhabditis elegans (C. elegans), using different concentrations. The results showed that maca extracts were not toxic to the model at concentrations below 100 mg L-1. However, higher concentrations caused high mortality, growth disturbances, oxidative stress and lipid accumulation. Black maca extracts increased the reproduction of C. elegans by increasing the number of offspring in C. elegans, both in aqueous and ethanolic extracts. On the other hand, ethanolic extracts produced an increase in the expression of genes related to oxidative stress, indicating a mild stressor behavior of the same. C. elegans represents an established model for evaluating the biological properties of nutraceutical plants of biological interest and can be used in the search for antioxidant activity of L. meyenii (hypocotyl), as well as it can be used in future studies to identify some metabolites involved in each biological property and to understand the biochemical and molecular mechanisms involved in these properties.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84863941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical composition and antioxidant activity of leaf essential oil from Calyptranthes concinna DC. (Myrtaceae) 胭脂草叶精油的化学成分及抗氧化活性。(桃金娘科)

Acta Scientiarum. Biological Sciences Pub Date : 2022-10-28 DOI: 10.4025/actascibiolsci.v44i1.62438

Fábio Antônio Antonelo, M. S. Rodrigues, Américo Wagner Júnior, P. F. Montanher

{"title":"Chemical composition and antioxidant activity of leaf essential oil from Calyptranthes concinna DC. (Myrtaceae)","authors":"Fábio Antônio Antonelo, M. S. Rodrigues, Américo Wagner Júnior, P. F. Montanher","doi":"10.4025/actascibiolsci.v44i1.62438","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.62438","url":null,"abstract":"The biodiversity found in Brazilian’s ecosystems brings the possibility of discovering new natural products with wide application potentials. However, knowing their availability and chemical composition is crucial. Thus, the aim of this study was to evaluate the extraction yield, chemical composition and antioxidant activity of essential oil from fresh leaves of Calyptranthes concinna DC., a native species of Myrtaceae occurring in Brazilian Atlantic Rain Forest. Plant samples were collected in Southeastern Brazil and the essential oil was extracted by hydrodistillation. The chemical composition was evaluated by Gas Chromatography associated with Mass Spectrometry and antioxidant activity was measured using ABTS, DPPH and FRAP methods. The extraction yield obtained was 0.015% (v), and the chemical composition revealed elemicin, a phenylpropanoid as the major component (36.46%). Still, β-caryophyllene (16.94%), germacrene B (8.28%) and spathulenol (7.33%) proved to be relevant for the same essential oil. Antioxidant activity was obtained for ABTS and DPPH radical scavenge (134.82 ± 2,9 and 93.70 ± 1.7 µM TE mL-1, respectively) and FRAP (11.31 ± 0.2 µM FeSO4 mL-1 OE), revealing hydrogen-donation as the main antioxidant mechanism. To our knowledge, this is the first report of antioxidant activity of C. concinna essential oil. The product presented compounds of great relevance, with possibilities of application in different areas including food, agriculture and pharmaceutical segments","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"132 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79637125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Lema quadrivittata (Coleoptera: Chrysomelidae): morphological description and its use as a biological control agent of Commelina benghalensis L. 四棱蝇甲(鞘翅目:金蝇科):形态描述及其防制作用。

Acta Scientiarum. Biological Sciences Pub Date : 2022-10-28 DOI: 10.4025/actascibiolsci.v44i1.60418

Juliana Elias de Oliveira, L. Dalvi, F. D. Oliveira, D. Pratissoli, G. Andrade, J. Pedrosa

{"title":"Lema quadrivittata (Coleoptera: Chrysomelidae): morphological description and its use as a biological control agent of Commelina benghalensis L.","authors":"Juliana Elias de Oliveira, L. Dalvi, F. D. Oliveira, D. Pratissoli, G. Andrade, J. Pedrosa","doi":"10.4025/actascibiolsci.v44i1.60418","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.60418","url":null,"abstract":"Weed infestation is one of the main challenges in crop management. The environmental, economic and social impacts attributed to pesticides lead to the search for new sustainable possibilities for the management of these plants, especially for Commelina benghalensis, which is considered one of the worst weeds in the world. This work consists of a description of the external morphology of the insect Lema quadrivittata and its potential use as a biological regulator of C. benghalensis, a weed plant, which suffers effective injuries by this natural enemy. Plots with high infestation of C. benghalensis were inspected to find plant damage and its possible causes. Upon identifying the occurrence of L. quadrivittata, it was decided to cultivate C. benghalensis in a greenhouse in order to observe the interaction of the insect with the plant. In this sense, during the study, we identified the insect L. quadrivittata by describing its morphological structures of adults and larvae and its interaction with C. benghalensis as a biological control agent. This work is an approach to the attack of L. quadrivittata on C. benghalensis. Future studies are needed to indicate the potential of this insect as a biological regulator of this weed from the knowledge of its behavior, morphology and evaluations of potential hosts.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84427977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay 用大蒜浸出液生物测定法评价迷迭香精油的细胞毒性和诱变潜力

Acta Scientiarum. Biological Sciences Pub Date : 2022-10-28 DOI: 10.4025/actascibiolsci.v44i1.62665

Marcos Vinícius Cosme, N. M. F. Nunes, A. P. Oliveira, Elisângela Cláudia Alves de Oliveira

{"title":"Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay","authors":"Marcos Vinícius Cosme, N. M. F. Nunes, A. P. Oliveira, Elisângela Cláudia Alves de Oliveira","doi":"10.4025/actascibiolsci.v44i1.62665","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.62665","url":null,"abstract":"The species Rosmarinus officinalis L. (rosemary) is an herb from the Lamiaceae family, widely used in cooking as a food preservative, seasoning or condiment. It also stands out for its therapeutic properties, mainly presenting antioxidant, antibacterial and antitumor activity. The aim of this study was to evaluate the cytotoxic and mutagenic activity of R. officinalis L. essential oil through the Allium cepa bioassay. This test constitutes an excellent plant model routinely used due to its sensitivity, low cost and good correlation with test systems in mammals. The defined concentrations for carrying out the test were 750, 243, 81 and 27 µg mL-1. Five bulbs were used, 4 roots of each, measuring approximately 2 cm, and they were analyzed on two slides. All assays were performed at least in triplicate and compared to the negative control. The statistical test of analysis of variance (ANOVA with a fixed factor) was used, followed by Tukey's multiple comparisons test, for p < 0.05. For this purpose, the GraphPad Prism program (version 6.0) was used. The results showed a cytotoxic and mutagenic effect for all concentrations used of the essential oil of R. officinalis L. However, it is important to conduct further research using other genotoxicological tests with different endpoints and at different concentrations, in order to clarify the interaction of the essential oil of the species R. officinalis L. with the genetic material of the cell and its possible mechanism of action.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78965005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of bacterial co-culture and organic amendments on the bioremediation of hydrocarbons in a soil contaminated with spent engine oil 细菌共培养和有机改良剂对废机油污染土壤中碳氢化合物生物修复的影响

Acta Scientiarum. Biological Sciences Pub Date : 2022-10-28 DOI: 10.4025/actascibiolsci.v44i1.62289

A. Adeleye, M. Yerima, Micheal Edem Nkereuwem, V. Onokebhagbe, G. P. Shiaka, A. O. Amoo, Catherine Iyabo Asaju, B. Yalwaji, Saheed Mohammed Ishaq

{"title":"Effect of bacterial co-culture and organic amendments on the bioremediation of hydrocarbons in a soil contaminated with spent engine oil","authors":"A. Adeleye, M. Yerima, Micheal Edem Nkereuwem, V. Onokebhagbe, G. P. Shiaka, A. O. Amoo, Catherine Iyabo Asaju, B. Yalwaji, Saheed Mohammed Ishaq","doi":"10.4025/actascibiolsci.v44i1.62289","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.62289","url":null,"abstract":"Bioenhancement of hydrocarbonoclastic microorganisms with suitable nutrients has a huge impact in achieving positive bioremediation of polluted environments. This study was conducted to assess the bio-enhancing effect of some organic amendments on Streptococcus pyogenes and Enterococcus faecalis co-culture with a view to remediating spent engine oil (SEO) contaminated soil. Top soil (1.5 kg) was autoclaved and thereafter contaminated with SEO at three levels. The contaminated soil was inoculated with bacterial co-culture (150 mL) and subsequently bioenhanced with compost, processed cocoa pod husk (CPH) and cow dung. The factorial experiment was laid out in completely randomized design. Concentrations of total petroleum hydrocarbon (TPH) and selected polycyclic aromatic hydrocarbons (PAH) were estimated on the first day, 5th week and 10th week of incubation. Results obtained show that bacterial co-culture bioenhanced with compost produced the most significant TPH reductions (1318 and 261 mg kg-1) on 10% SEO contaminated soil at the 5th and 10th week respectively (p<0.05). Again, bacterial co-culture bioenhanced with compost produced the most significant PAH reductions (65.9 and 55.8 mg kg-1) on 10% SEO contaminated soil at the 5th and 10th week respectively (p<0.05). The significant bioremediation capabilities exhibited by the bacterial co-culture bioenhanced with organic amendments in this study has made these bioremediation agents potential candidates in remediating soils impacted with petroleum hydrocarbons.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"47 10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82187499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity in Cichla piquiti and cross-amplification for Cichla kelberi in the Serra da Mesa reservoir, Goiás, Brazil 巴西Serra da Mesa水库皮奇拉遗传多样性及克尔贝拉杂交扩增

Acta Scientiarum. Biological Sciences Pub Date : 2022-10-28 DOI: 10.4025/actascibiolsci.v44i1.58294

R. C. P. Faquim, Ramilla dos Santos Braga Ferreira, T. G. Castro, Leonel Silva, M. Telles

{"title":"Genetic diversity in Cichla piquiti and cross-amplification for Cichla kelberi in the Serra da Mesa reservoir, Goiás, Brazil","authors":"R. C. P. Faquim, Ramilla dos Santos Braga Ferreira, T. G. Castro, Leonel Silva, M. Telles","doi":"10.4025/actascibiolsci.v44i1.58294","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.58294","url":null,"abstract":"Species Cichla piquiti and Cichla kelberi are found in the Serra da Mesa reservoir, Goiás and are sedentary with diurnal habits. This study aimed to evaluate the magnitude and distribution of genetic variability in subpopulations of C. piquiti with specific microsatellite loci and to test transferability in other microsatellite markers for C. kelberi. We analyzed 99 individuals of C. piquiti from seven points to evaluate genetic diversity and structure with 10 microsatellite loci. Transferability of 75 loci was tested in C. kelberi to increase microsatellite markers available. Genetic structure was assessed with Bayesian clustering. Global FST for C. piquiti was weak (0.056), but FIS (0.598) and FIT (0.621) were significantly high, indicating that the mating system has a strong influence on the organization of genetic variability with most mating among related. Two genetic groups were evidenced with most individuals allocated to a single group. Transferability of microsatellite loci for C. kelberi had low polymorphism. The level of genetic diversity was low, increasing inbreeding and suggesting that few individuals of C. piquiti colonized the reservoir during its installation due founder effect. Other factors as reproductive behavior and overfishing can act to decrease genetic diversity. Therefore, we reinforce the need for genetic monitoring to avoid loss of genetic diversity that can be intensified both construction of hydropower plants and ecological and reproductive aspects in some fish species.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84489670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0