{"title":"Using canonical correlation analysis to understand the rumen biohydrogenation patterns of linoleic and alpha-linolenic acids in the rumen fluid of bovines","authors":"Julián Andrés Castillo Vargas","doi":"10.4025/actascianimsci.v44i1.57724","DOIUrl":"https://doi.org/10.4025/actascianimsci.v44i1.57724","url":null,"abstract":"The objective of this study was to determine the multivariate relationship among linoleic acid, alpha-linolenic acid, and their main rumen biohydrogenation (BH) intermediates and products in bovine rumen fluid using canonical correlation analysis (CCA). A dataset consisting of 1177 observations generated by 107 in vitro rumen incubation systems of pure and mixed linoleic acid (18:2-c9, c12) and alpha-linolenic acid (18:3-c9, c12, c15) was gathered. Two canonical variates were defined: A: composed of the nine main BH intermediates and products (18:2-c9, t11; 18:2-t11, c15; 18:1-t11; 18:1-t9; 18:1-t6; 18:1-c11; 18:1-c6; 18:1-c9; 18:0) of 18:2-c9, c12 and 18:3-c9, c12, c15 and B: composed of 18:2-c9, c12 and 18:3-c9, c12, c15. Two canonical functions between A and B with significant canonical correlations (R1=0.990 and R2=0.738; p <0.01) were obtained. However, only the first function was selected for CCA. Exploration of canonical loadings for first function, revealed the following quantitative significance (absolute value) order for fatty acids (FA) within their respective canonical variates: A: 18:0(0.958)>18:1-t9(0.837)>18:1-c11(0.835)>18:1-c6(0.824)>18:1-t11(0.747)>18:1-c9(0.738)>18:1-t6(0.415)>18:2-t11, c15(0.387)> 18:2-c9, t11(0.239); B: 18:2-c9, c12(0.667)>18:3-c9, c12, c15(0.488). The CCA showed that 18:2-c9, c12 has a greater contribution than that of 18:3-c9, c12, c15 on the production of the aforementioned BH intermediates, in which 18:0, as well as the groups of 18:1 cis and trans-FA were mainly affected.","PeriodicalId":7149,"journal":{"name":"Acta Scientiarum. Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90661859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. EL-Deeb, Megahed Abdel-Gawad, M. Abdel-Hafez, F. Saba, E. M. Ibrahim
{"title":"Effect of adding Spirulina platensis algae to small ruminant rations on productive, reproductive traits and some blood components","authors":"M. EL-Deeb, Megahed Abdel-Gawad, M. Abdel-Hafez, F. Saba, E. M. Ibrahim","doi":"10.4025/actascianimsci.v44i1.57546","DOIUrl":"https://doi.org/10.4025/actascianimsci.v44i1.57546","url":null,"abstract":"This experiment conducted using 20 Rahmani ewes at the last third of pregnancy in two equal groups. One group served as control, while the other group received Spirulina platensis (SP) at the rate of 0.5 gm 10 kg-1 live body weight. The objective was to find out the effect of adding Spirulina platensis algae to small ruminant rations on reproductive and productive traits and blood components of sheep. The experiments lasted for 120 days for both dams and their lambs after weaning. The findings proved that adding SP in ewes' diets had no effect on the average of live body weight change. Average milk yield was significantly (p <0.01) higher in the treatment group than the control. Lamb's birth weight and daily body gain of the treated group were significantly (p <0.01) higher than the control. Blood and serum picture profile of ewes were significantly higher when fed SP additive than the control. It could be concluded that the addition of SP to the ration of sheep positively preserved their health, productive and reproductive status as well as their lambs' growth rate. Also the additive improved the economic efficiency of treated animals by about 53.13%.","PeriodicalId":7149,"journal":{"name":"Acta Scientiarum. Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72944055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thiago Brandão Cavalheiro, Juan Carlos Letelier Carvajal, R. F. P. Lucena, Carlos Tadeu Carvalho do Nascimento, Thaís Teresa Brandão Cavalheiro Ribeiro
{"title":"Water parameters correlated with the zootechnical performance of shrimp Litopenaeus vannamei grown in oligohaline waters","authors":"Thiago Brandão Cavalheiro, Juan Carlos Letelier Carvajal, R. F. P. Lucena, Carlos Tadeu Carvalho do Nascimento, Thaís Teresa Brandão Cavalheiro Ribeiro","doi":"10.4025/actascianimsci.v44i1.57700","DOIUrl":"https://doi.org/10.4025/actascianimsci.v44i1.57700","url":null,"abstract":"The present study aimed to correlate the influence of water quality, cultivation time, area, and density of ponds on the zootechnical performance of shrimp Litopenaeus vannamei in oligohaline waters. It was demonstrated that, under the conditions of this research, better control of temperature, pH and water alkalinity can be employed. Ammonia levels were within the normal range and correlated positively with the growth rate (ρ = 0.69859) and production (ρ = 0.66362) of the ponds. There was also a positive correlation between cultivation time and pond density with shrimp weight (ρ = 0.75305 and 0.68933) and pond productivity (ρ = 0.74763 and 0.79367, respectively). Negative correlations were observed between: I) pH with shrimp survival (ρ = -0.73238) and pond production (ρ = -0.68696); II) alkalinity and pond production (ρ = -0.66162). The results show that the cultivation of shrimp L. vannamei in oligohaline waters is viable and there is potential for increased productivity associated with better hydrological monitoring and use of higher density and cultivation time by some producers.","PeriodicalId":7149,"journal":{"name":"Acta Scientiarum. Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72379301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Renato Mattos Fernandes, Maria Luiza dos Santos Maciel, A. V. Souza, L. G. Amaral, N. Amaral
{"title":"Evaluation of an automated temperature control equipment for lactating sows","authors":"Renato Mattos Fernandes, Maria Luiza dos Santos Maciel, A. V. Souza, L. G. Amaral, N. Amaral","doi":"10.4025/actascianimsci.v44i1.54981","DOIUrl":"https://doi.org/10.4025/actascianimsci.v44i1.54981","url":null,"abstract":"The present study aimed to develop and evaluate an automated cooling control system (ATCE) for sows in the farrowing phase. The experiment was conducted at the Swine Production Unit, Federal Institute of Education, Science and Technology, Campus Machado, Machado, state of Minas Gerais. Sixteen sows and their offspring were evaluated, eight treated and eight controls, in a randomized block design, with eight replications. Surface temperature, body temperature and respiratory rate of the animals were evaluated, in addition to floor temperature and performance parameters. The use of ATCE decreased the floor temperature (12h) and neck temperature (12 and 16h) (p < 0.10). In addition, there was a reduction in rectal temperature (12h) and respiratory rate (12 and 16h) in ATCE animals (p < 0.10). No significant differences were detected in the coefficient of variation (CV) of birth weight, but at weaning the CV of piglet weight was 26.3% lower in animals subjected to the ATCE (p = 0.079). Furthermore, the difference in CV between birth and weaning was also lower with the use of ATCE (p = 0.015). It can be concluded that the use of ATCE has a positive influence on the thermal comfort of females, in addition to improving litter uniformity.","PeriodicalId":7149,"journal":{"name":"Acta Scientiarum. Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85873964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. O. Abioja, Stephen Apuu, J. Daramola, M. Wheto, O. F. Akinjute
{"title":"Semen quality and sperm characteristics in broiler breeder cockerels fed vitamin E during hot season","authors":"M. O. Abioja, Stephen Apuu, J. Daramola, M. Wheto, O. F. Akinjute","doi":"10.4025/actascianimsci.v44i1.56848","DOIUrl":"https://doi.org/10.4025/actascianimsci.v44i1.56848","url":null,"abstract":"Thirty-two Arbor Acres (AA) cockerels aged 27 weeks were used to determine the effect of vitamin E (VE) on the semen characteristics and sperm quality during hot season. The birds were fed diets containing 0, 50, 100 and 150mg VE kg-1 feed. There were 4 replicates and two birds per replicate. Semen collection was done thrice a week for three weeks in all birds. Dietary vitamin E significantly (p < 0.05) increased the semen volume (SV) and number of sperm cells (NS) in AA cockerels. CONTROL birds (0.71 mL) had lower SV than birds fed 150 mg VE (0.94 mL). Birds on 100 and 150 mg VE recorded similar NS, which was higher than 0 and 50 mg VE groups. The least NS was in the CONTROL group. Sperm abnormality gradually (p < 0.05) decreased as dietary VE increased from 0 to 150 mg. Significant (p < 0.01) effect of dietary VE was recorded in percentage live sperm (PLS). Semen from cockerels on VE treatment had higher PLS than the CONTROL. In conclusion, it may be beneficial to supplement the broiler breeder cockerel diet with VE up to 150 mg kg-1 feed during hot season as it increased SV, NS and PLS and decreased sperm abnormality.","PeriodicalId":7149,"journal":{"name":"Acta Scientiarum. Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78648719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Masoomeh Zare, M. Sadeghi, J. Ahmadpanah, Abbas Safari, M. Moridi
{"title":"Association of Palm gene mutations with structure and function of paralemmin proteins in Lori-Bakhtiari and Zel sheep Breeds","authors":"Masoomeh Zare, M. Sadeghi, J. Ahmadpanah, Abbas Safari, M. Moridi","doi":"10.4025/actascianimsci.v44i1.57287","DOIUrl":"https://doi.org/10.4025/actascianimsci.v44i1.57287","url":null,"abstract":"The aim of current study was to survey genetic variability of PALM gene’s exon 3 and 4 by PCR-SSCP and DNA sequencing in Zel and Lori Bakhtiari sheep breeds. The SIFT (Sorting Intolerant from Tolerant) and PHyre2 program were used to predict the possible impact of amino acid substitutions on performance and structure of the paralemmin protein. A total of 140 animal's from 2 Iranian sheep breeds with different fat metabolisms, Lori-Bakhtiari and Zel sheep breeds were considered. The results showed that there are two polymorphic sites including a nonsynonymous substitution and an insertion mutation (49bp). Non-synonymous mutation deduced Thr20Ala amino acid exchange and ensuing two different structures for paralemmin protein that could be potentially affect protein structure and function during the interaction with glutamate in the cytosolic surface of plasma membrane. PALM gene, according to evolutionary path, is classified into two separate categories. In first covey, Gallus gallus and in second one, other species in several branches, so that the sequence of cow and sheep is placed in a sub-branch which forms a clade beside goat. Comparison of illustrated coding region sequences, PALM gene among different species, is of orthologous which are derived from a common ancestor.","PeriodicalId":7149,"journal":{"name":"Acta Scientiarum. Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79206492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marllon José Karpegianne de Oliveira, A. Geraldo, S. Lemke, J. K. Valentim, É. J. Fassani, Jordanna Barbosa Ferreira De Carvalho, D. A. Miranda, Eliane Aparecida da Silva
{"title":"Emulsifiers in diets with energy reduction for laying hens","authors":"Marllon José Karpegianne de Oliveira, A. Geraldo, S. Lemke, J. K. Valentim, É. J. Fassani, Jordanna Barbosa Ferreira De Carvalho, D. A. Miranda, Eliane Aparecida da Silva","doi":"10.4025/actascianimsci.v44i1.54702","DOIUrl":"https://doi.org/10.4025/actascianimsci.v44i1.54702","url":null,"abstract":"Emulsifiers in animal diets are an interesting and effective strategy for nutritionists to improve the digestion of dietary lipids and, consequently, provide better performance. This study aimed to evaluate the effect of the supplementation of diets with emulsifier based on mono- and diglycerides (120 g t-1 of feed) associated with different energy levels (2,775; 2,725; 2,675 and 2,625 kcal of metabolizable energy kg-1 of feed) on performance and egg quality of Hisex Brown laying hens. The laying hens were distributed in 50 experimental plots with eight birds each. A completely randomized design with five treatments and ten replicates/treatments was used. The treatments in this study were: control diet (C) with 2,775 kcal of metabolizable energy (ME) kg-1 of feed; C + E: diet C supplemented with an emulsifier (E) at 120 g t-1 of feed; diet C with reduction of 50 kcal ME and supplemented with E (NC50 + E); diet C with reduction of 100 kcal ME and supplemented with E (NC100 + E); and diet C with reduction of 150 kcal ME and supplemented with E (NC150 + E). All diets had the same inclusion level of degummed soybean oil (2%). There was no influence of the treatments on the performance and quality of the eggs (p >0.05). The current study has shown that diets supplemented with emulsifiers based on mono- and diglycerides (120 g t-1) allows reducing the metabolizable energy level up to 150 kcal kg-1 of feed, without impairment on performance and egg quality of Hisex Brown laying hens","PeriodicalId":7149,"journal":{"name":"Acta Scientiarum. Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87432627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. T. Malerbo-Souza, Carlos Frederico Silva da Costa, A. C. Pimentel, M. O. Andrade, Rodrigo Alves de Siqueira, Robin César Barros da Silva, Camila Malerbo de Souza, Felipe Gusmão de Souza
{"title":"Stingless bee Trigona spinipes (Hymenoptera: Apidae) behavior on chayote flowers (Sechium edule)","authors":"D. T. Malerbo-Souza, Carlos Frederico Silva da Costa, A. C. Pimentel, M. O. Andrade, Rodrigo Alves de Siqueira, Robin César Barros da Silva, Camila Malerbo de Souza, Felipe Gusmão de Souza","doi":"10.4025/actascianimsci.v44i1.56760","DOIUrl":"https://doi.org/10.4025/actascianimsci.v44i1.56760","url":null,"abstract":"This experiment aimed to evaluate the forage species and their effect on fruit production of the chayote crop. For this, the culture was under observation in the first ten minutes at each time, from 7:00 am to 6:00 pm, with three repetitions, in each year studied (1994, 2001, 2009 and 2016). With the exception of 2009, Trigona spinipes stingless bees were frequent and constant insects in these flowers between 8:00 am and 1:00 pm, visiting mainly male flowers for nectar collection and due to foraging behavior this stingless bee may be considered a pollinator of the chayote.","PeriodicalId":7149,"journal":{"name":"Acta Scientiarum. Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88027917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tatiana Soares dos Santos, Ernane Souza, N. Bragagnolo, Antonio Rosendo da Costa, José Jordão Filho, N. M. Almeida
{"title":"Essential fatty acids in farmed tambaqui (Colossoma macropomum) from the Brazilian Amazon Area","authors":"Tatiana Soares dos Santos, Ernane Souza, N. Bragagnolo, Antonio Rosendo da Costa, José Jordão Filho, N. M. Almeida","doi":"10.4025/actascianimsci.v44i1.57090","DOIUrl":"https://doi.org/10.4025/actascianimsci.v44i1.57090","url":null,"abstract":"The goal of this study was to determine the essential fatty acids of the total lipids of the fillet, head and orbital cavity tissue from farmed tambaqui (Colossoma macropomum) fish from a Brazilian Amazon area. The tambaqui were acquired from different fish farms in the Roraima state, located at Western Brazilian Amazon. The meat, the head and the fatty tissue from orbital cavity were dissected for lipid extraction and analysis of fatty acids by gas chromatography. The fatty acids were quantified in mg g-1 of total lipids using C23:0 as an internal standard. The nutritional quality of the lipids was determined by using the atherogenicity and thrombogenicity indices, and also by the ratio between hypocholesterolemic / hypercholesterolemic fatty acids. The orbital cavity tissue had the higher concentration amount of linoleic and α-linolenic acid, whereas the fillet had higher docosahexaenoic acid (DHA). The eicosapentaenoic acid (EPA) concentration was: 1.28, 0.97, 1.71 mg g-1 of total lipids, in the filet, in head, and in orbital cavity tissue, respectively. All essential fatty acids were detected in the three parts analyzed. The nutritional quality of the total lipids from the head and from the orbital cavity tissue was similar to the fillet.","PeriodicalId":7149,"journal":{"name":"Acta Scientiarum. Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79474465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. H. Nameghi, Ommolbanin Edalatian, R. Bakhshalinejad
{"title":"A blend of thyme and rosemary powders with poultry by-product meal can be used as a natural antioxidant in broilers","authors":"A. H. Nameghi, Ommolbanin Edalatian, R. Bakhshalinejad","doi":"10.4025/actascianimsci.v44i1.57126","DOIUrl":"https://doi.org/10.4025/actascianimsci.v44i1.57126","url":null,"abstract":"A total of 500, 14-d old male Ross 308 broilers were allocated into five treatments (4 replicates each) including: a negative (NC) and positive control diet (PC) which supplemented without and with 7% poultry by-product meal (PBPM), respectively and three levels of additive supplementation of thyme and rosemary powders in the basal diets: 0.750% rosemary powder (PCR); 0.375% thyme powder + 0.375% rosemary powder (PCRT), and 0.750% thyme powder (PCT) rosemary powder. The PCRT diet improved (p <0.05) average daily gain and feed conversion ratio by 5.62 and 10.37% compared to PC, respectively. The serum concentration of lipids (triglycerides, cholesterol, high-density lipoprotein, and low-density lipoprotein) was decreased while the ileal villus height and ratio of villus height to crypt depth as well as ileal microbiota population were improved (p <0.05) in response to inclusion PCRT diet compared to PC diet. The blood serum concentration of malondialdehyde was statistically decreased (p <0.05) by supplementation of PCRT in broiler diets. Our results suggested that supplementation of a blend of thyme and rosemary powders at the level of 0.375% of each into broiler diet during d 14-42 has merit to be used as a natural antioxidant in diets formulated by PBPM.","PeriodicalId":7149,"journal":{"name":"Acta Scientiarum. Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72805862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}