Acta microbiologica et immunologica Hungarica最新文献_第9页

Detection of carbapenem resistant Pseudomonas aeruginosa co-harboring blaVIM-2 and blaGES-5 in burn patients. 烧伤患者中携带blaVIM-2和blaGES-5的碳青霉烯耐药性铜绿假单胞菌的检测。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2023-07-25 Print Date: 2023-09-21 DOI: 10.1556/030.2023.02089

Souhir Hmissi, Anis Raddaoui, Siwar Frigui, Mohamed Salah Abbassi, Wafa Achour, Yosra Chebbi, Lamia Thabet

{"title":"Detection of carbapenem resistant Pseudomonas aeruginosa co-harboring blaVIM-2 and blaGES-5 in burn patients.","authors":"Souhir Hmissi, Anis Raddaoui, Siwar Frigui, Mohamed Salah Abbassi, Wafa Achour, Yosra Chebbi, Lamia Thabet","doi":"10.1556/030.2023.02089","DOIUrl":"10.1556/030.2023.02089","url":null,"abstract":"Pseudomonas aeruginosa is one of the major infectious agents in burn patients. Globally, high rates of antimicrobial resistance in P. aeruginosa have been reported, which is a cause of concern. The objective of this study was to determine the rate of resistance to carbapenems in P. aeruginosa isolates recovered from burn patients in Tunisia, to search genes encoding for carbapenemases and to determine their epidemiological markers (serotypes). A retrospective study was conducted in the Burn Intensive Care Unit (BICU) of the Trauma and Burn Centre of Ben Arous, Tunisia, and P. aeruginosa isolates collected from burn patients, from January to December 2018 were investigated. Carbapenemase screening was performed by Carbapenem Inactivation Method (CIM) and by EDTA-disk test for all carbapenem resistant isolates. Genes encoding carbapenemases (blaVIM, blaIMP, blaGES, blaNDM, and blaKPC) were investigated by PCR and selected carbapenemase genes were sequenced. During the study period, 104 non duplicated P. aeruginosa isolates were recovered. Most of them were isolated from skin samples (45.1%) and blood culture (22.1%) and belonged to O:11 (19.2%), O:12, and O:5 (12.5%, each) serotypes. High rates of resistance were observed for carbapenems (64.4%). Among the 67 carbapenem resistant isolates, 58 (86.5%) harbored blaVIM gene and 55 (82%) blaGES gene; in addition, 48 (71.6%) co-harbored blaVIM and blaGES genes. After sequencing, the blaVIM-2 and blaGES-5 gene variants were identified in seven randomly selected isolates. To the best of our knowledge, this is the first description of P. aeruginosa simultaneously harboring blaVIM-2 and blaGES-5 genes.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"199-205"},"PeriodicalIF":1.5,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10228654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Molecular epidemiology and characterization of endemic, epidemic and imported hepatitis A virus (HAV) strains in Hungary (2003-2022). 匈牙利地方性、流行性和输入性甲型肝炎病毒株的分子流行病学和特征（2003-2022）。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2023-07-25 Print Date: 2023-09-21 DOI: 10.1556/030.2023.02099

Gábor Reuter, Ákos Boros, Péter Pankovics

{"title":"Molecular epidemiology and characterization of endemic, epidemic and imported hepatitis A virus (HAV) strains in Hungary (2003-2022).","authors":"Gábor Reuter, Ákos Boros, Péter Pankovics","doi":"10.1556/030.2023.02099","DOIUrl":"10.1556/030.2023.02099","url":null,"abstract":"Hepatitis A virus (HAV) is one of the most important etiological agents of acute viral hepatitis but comprehensive molecular epidemiological study with chrono-phylogeographical data are not available from Hungary.Between 2003 and 2022, a total of 8,307 HAV infections were registered officially in Hungary of which 400 (4.8%) HAV IgM antibody-positive serum samples were collected countrywide. HAV genomic RNA was successfully detected in 216/400 (54%) sera by RT-PCR subsequently confirmed by sequencing. The complete nucleotide sequences of VP1 region were determined in 32 representative HAV strains. Based on the sequence analysis, 150 (69.4%) strains were characterized as HAV sub-genotype IA and 66 (30.6%) as sub-genotype IB, respectively. Based on the combined epidemiological and molecular data, epidemic, endemic, and imported HAV strains were also characterized. The first two registered countrywide outbreaks started among men-sex-with men (MSM) in 2011 (sub-genotype IA) and 2021 (sub-genotype IB), the continuously circulating endemic/domestic HAV strain (sub-genotype IA) in East Hungary and the travel-related sub-genotype IB strains from Egypt should be highlighted. All HAV strains are deposited in the HAVNET database (https://www.rivm.nl/en/havnet).In this 20-year-long comprehensive molecular epidemiological study, we report the genetic characterization and geographic distribution of endemic, epidemic and imported HAV strains for the first time in Hungary with continuous co-circulation of sub-genotypes IA and IB HAV strains since 2003. These data provide basic information about the HAV situation in the country in an international context and can promote more effective national public health intervention strategies for the prevention of HAV transmissions and infections.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"246-251"},"PeriodicalIF":1.5,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10228652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergence of colistin resistant Acinetobacter baumannii clonal complex 2 (CC2) among hospitalized patients in Iran. 伊朗住院患者中出现耐粘菌素鲍曼不动杆菌克隆复合物2（CC2）。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2023-07-24 Print Date: 2023-09-21 DOI: 10.1556/030.2023.02057

Bahareh Hajihashemi, Ali Abbasi, Dariush Shokri

{"title":"Emergence of colistin resistant Acinetobacter baumannii clonal complex 2 (CC2) among hospitalized patients in Iran.","authors":"Bahareh Hajihashemi, Ali Abbasi, Dariush Shokri","doi":"10.1556/030.2023.02057","DOIUrl":"10.1556/030.2023.02057","url":null,"abstract":"Acinetobacter baumannii is a major causative agent of serious nosocomial infections. This study was carried out to investigate the molecular characterization of colistin resistant isolates of A. baumannii from hospitalized patients, based on multilocus sequence typing (MLST). A cross-sectional study was conducted to collect A. baumannii from clinical samples in Isfahan from 2021 to 2022. Isolates were identified as A. baumannii using biochemical tests and PCR of blaOXA-51. Antibiotic susceptibility testing was carried out using the Kirby-Bauer method and minimum inhibitory concentration (MIC) values were determined for colistin. Additionally, MLST was performed according to the Pasteur scheme to assess the relationship between colistin resistant A. baumannii. A total of 70 non-repetitive A. baumannii isolates were obtained from different clinical samples. MIC results showed that seven A. baumannii isolates were resistant to colistin. The antibiotic susceptibility pattern revealed that all seven colistin resistant strains were resistant to all tested antibiotics. Based on MLST analysis, the colistin resistant isolates were assigned to five unique STs namely, ST2 (3; 42.9%) followed by ST78 (1; 14.3%), ST1077 (1; 14.3%), ST415 (1; 14.3%) and ST391 (1; 14.3%). Among them ST2, ST391 and ST415 belong to clonal complex 2. Colistin resistant A. baumannii ST2 is the main circulating clone in clinical settings in Iran, but additionally ST415, ST391, and ST1077 are found for the first time in our country. Intensive control procedures and strict adherence to surveillance programs are recommended to decrease the spread of carbapenem and colistin resistant A. baumannii strain.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"213-219"},"PeriodicalIF":1.5,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9862551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of NDM-1 and IMP-8 carbapenemase producing Raoultella planticola clinical isolates. NDM-1和IMP-8产碳青霉烯酶植物拉乌尔氏菌临床分离株的分析。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2023-07-21 Print Date: 2023-09-21 DOI: 10.1556/030.2023.02078

Qingyu Wang, Xinyi Wang, Chunling Chen, Li Zhao, Jie Ma, Kai Dong

{"title":"Analysis of NDM-1 and IMP-8 carbapenemase producing Raoultella planticola clinical isolates.","authors":"Qingyu Wang, Xinyi Wang, Chunling Chen, Li Zhao, Jie Ma, Kai Dong","doi":"10.1556/030.2023.02078","DOIUrl":"10.1556/030.2023.02078","url":null,"abstract":"Object of our study was to analyze the carriage of resistance genes in carbapenem-resistant Raoultella planticola (CRRP) by whole genome sequencing (WGS). Three strains of CRRP (named WF0027, WF3597 and WF3648) were collected for clinical analysis and susceptibility of antimicrobial agents was determined. The WGS of three strains was done by Illumina platform and strain identification was performed by average nucleotide identity, and the antibiotic resistance genes carried by the three strains were detected by ABRicate software. Whole genome data of 46 CRRP strains were downloaded from the National Center for Biotechnology Information (NCBI) database, and the evolutionary tree was constructed by genomic single nucleotide polymorphism together with this study strains. Antimicrobial susceptibility testing revealed that WF3597 and WF3648 were susceptible to tigecycline and colistin, while exhibited resistance to 24 antimicrobial agents. WF0027 was resistant to 18 antimicrobial agents. A total of 25 resistance genes were identified using ABRicate software. WF0027 carried blaIMP-8, whereas WF3597 and WF3648 carried blaNDM-1 carbapenem resistance gene. As predicted by the PlasmidFinder, WF3597 and WF3648 carried one plasmid IncFII(p14)_1_p14, whereas WF0027 carried five plasmids. Evolutionary tree results show all strains are clustered into six groups, the strains WF3597 and WF3648 belonged to the same evolutionary group (E clade) and WF0027 belonged to the F clade. Three CRRP strains in our study carried carbapenem resistance genes (blaNDM-1 or blaIMP-8) and were resistant to multiple antimicrobial agents, posing a significant challenge for clinical treatment.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"193-198"},"PeriodicalIF":1.5,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9850829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Clinical and microbiological characteristics of nocardiosis: A 5-year single-center study in Crete, Greece. 诺卡氏病的临床和微生物学特征：希腊克里特岛一项为期5年的单中心研究。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2023-07-17 Print Date: 2023-09-21 DOI: 10.1556/030.2023.02071

Sofia Maraki, Viktoria Eirini Mavromanolaki, Anna Kasimati, Dimitra Stafylaki, Effie Scoulica

{"title":"Clinical and microbiological characteristics of nocardiosis: A 5-year single-center study in Crete, Greece.","authors":"Sofia Maraki, Viktoria Eirini Mavromanolaki, Anna Kasimati, Dimitra Stafylaki, Effie Scoulica","doi":"10.1556/030.2023.02071","DOIUrl":"10.1556/030.2023.02071","url":null,"abstract":"Nocardiosis is a rare disease affecting both immunocompromised and immunocompetent hosts, presented in various clinical forms ranging from localized to disseminated infection. Aim of the present study was to investigate the clinical and microbiological characteristics of nocardiosis, antimicrobial resistance profiles, treatment, and outcomes of Nocardia infection over the last 5 years at our institution. The medical records and microbiological data of patients affected by nocardiosis and treated at the university hospital of Heraklion, Crete, Greece, between 2018 and 2022, were retrospectively analyzed. The isolates were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and through sequencing of 16S rRNA. Antimicrobial susceptibility for 17 agents was determined by E-test and results were interpreted according to CLSI guidelines. Among the 28 Nocardia isolates, eight species were identified, with Nocardia brasiliensis being the most prevalent (32.1%), followed by Nocardia otitidiscaviarum (25%), and Nocardia farcinica (14.3%). Skin and soft tissue infections were the most common presentations, noted in 13 (50%) patients, followed by pulmonary infection presented in 10 (38.5%) patients. Fifteen patients (57.7%) had at least one underlying disease, and 11 (42.3%) were on immunosuppressive or long-term corticosteroid treatment. Susceptibility rates of linezolid, tigecycline, amikacin, trimethoprim-sulfamethoxazole, moxifloxacin, and imipenem were 100, 100, 96.4, 92.9, 82.1, and 42.9%, respectively. The 26 patients in this study were treated with various antibiotics. Mortality rate was 3.8%, and the patient who died had disseminated infection. Since epidemiology and antimicrobial susceptibility are evolving, continuous surveillance is mandatory in order to initiate appropriate treatment in a timely manner.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"239-245"},"PeriodicalIF":1.5,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10203468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotypic and phenotypic insights into virulence factors of nosocomial Stenotrophomonas maltophilia isolates collected in Bulgaria (2011-2022). 保加利亚收集的医院嗜麦芽窄食单胞菌分离株毒力因子的基因型和表型见解（2011-2022）。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2023-07-04 Print Date: 2023-09-21 DOI: 10.1556/030.2023.02059

Tanya Strateva, Angelina Trifonova, Alexander Stratev, Slavil Peykov

{"title":"Genotypic and phenotypic insights into virulence factors of nosocomial Stenotrophomonas maltophilia isolates collected in Bulgaria (2011-2022).","authors":"Tanya Strateva, Angelina Trifonova, Alexander Stratev, Slavil Peykov","doi":"10.1556/030.2023.02059","DOIUrl":"10.1556/030.2023.02059","url":null,"abstract":"The present study aimed to explore the virulence characteristics in 221 Bulgarian nosocomial Stenotrophomonas maltophilia isolates (2011-2022) via screening for the presence of virulence genes, their mutational variability, and the corresponding enzyme activity. PCR amplification, enzymatic assays, whole-genome sequencing (WGS), and biofilm quantification on a polystyrene plate were performed. The incidence of virulence determinants was as follows: stmPr1 (encoding for the major extracellular protease StmPr1) 87.3%, stmPr2 (minor extracellular protease StmPr2) 99.1%, Smlt3773 locus (outer membrane esterase) 98.2%, plcN1 (non-hemolytic phospholipase C) 99.1%, and smf-1 (type-1 fimbriae, biofilm-related gene) 96.4%. The 1621-bp allele of stmPr1 was most frequently found (61.1%), followed by the combined allelic variant (17.6%), stmPr1-negative genotype (12.7%), and 868-bp allele (8.6%). Protease, esterase, and lecithinase activity was observed in 95%, 98.2%, and 17.2% of the isolates, respectively. The WGS-subjected isolates (n = 9) formed two groups. Five isolates possessed only the 1621-bp variant of stmPr1, higher biofilm formation ability (Optical Density at λ = 550 nm (OD550): 1.253-1.789), as well as a low number of mutations in the protease genes and smf-1. Three other isolates had only the 868-bp variant, weaker biofilm production (OD550: 0.788-1.108), and higher number of mutations within these genes. The only weak biofilm producer (OD550 = 0.177) had no stmPr1 alleles. In conclusion, the similar PCR detection rates did not allow differentiation of the isolates. In contrast, WGS permitted stmPr1 alleles-based differentiation. To the best of our knowledge, this is the first Bulgarian study presenting genotypic and phenotypic insights into virulence factors of S. maltophilia isolates.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"220-230"},"PeriodicalIF":1.5,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9812017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abstracts of the Annual Meeting of the Hungarian Society for Microbiology and the XV. Fermentation Colloquium 匈牙利微生物学会和第十五届微生物学会年会摘要。发酵讨论会

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2023-06-27 DOI: 10.1556/030.70.2023.001

引用次数: 0

Abstracts of the 19th International Congress of the Hungarian Society for Microbiology 第十九届匈牙利微生物学会国际大会摘要

4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2023-06-27 DOI: 10.1556/030.70.2023.002

引用次数: 1

Retraction note to: Treatment of methicillin resistant Staphylococcus aureus wound infection using vancomycin-loaded nanoparticles: An in vitro and in vivo study. 撤回说明：使用万古霉素负载纳米颗粒治疗耐甲氧西林金黄色葡萄球菌伤口感染：一项体外和体内研究。

IF 1.3 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2023-06-27 DOI: 10.1556/030.2023.02050

Hassan Thoulfikar A Alamir, Mustafa Gheni Taher, Mazin Razooqi Mohammed, Saja Hameed Kareem, Abduladheem Turki Jalil

引用次数: 0

Prevalence and distribution of high-risk Human Papillomavirus in Western Türkiye between 2019 and 2022. 2019年至2022年土耳其西部高危人类乳头瘤病毒的流行率和分布。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2023-06-22 Print Date: 2023-09-21 DOI: 10.1556/030.2023.02066

Gülfem Terek Ece, Doğukan Pira, Gökçe Lara Bodur

{"title":"Prevalence and distribution of high-risk Human Papillomavirus in Western Türkiye between 2019 and 2022.","authors":"Gülfem Terek Ece, Doğukan Pira, Gökçe Lara Bodur","doi":"10.1556/030.2023.02066","DOIUrl":"10.1556/030.2023.02066","url":null,"abstract":"Human Papillomavirus (HPV) is a primarily sexually transmitted human pathogen linked with the formation of anogenital warts and several types of cancer including cervical cancer, oropharyngeal cancer, penile cancer, and anal cancer. HPV genotypes are divided into high-risk and low-risk in accordance with their potential for carcinogenesis. The most notable high-risk genotypes are HPV-16 and HPV-18. In this study we report on the PCR analysis results of the cervical smear results of 328 females, and 50 males who have presented to a tertiary care university hospital in the port city of İzmir, Türkiye between August 2019 and August 2022. HPV-DNA positivity percentage was determined as 25.3% (96/378). 5 of 378 patients were HPV-18 positive (age range 31-80) whereas 27 patients were HPV-16 positive (age range 29-66). 64 patients were positive for other high-risk HPV types (age range 27-56). The positivity for HPV-16, HPV-18 and other high-risk HPV types were determined as 7.14%, 1.3% and 16.9% respectively. Our results demonstrate a 25.3% (96/378) positivity among tested patients moreover, out of 96 positive cases at least 32 are vaccine preventable. This study indicates the need for higher coverage of HPV vaccination in Western Türkiye.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"252-257"},"PeriodicalIF":1.5,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9680352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0