Zai-Song DING, Bao-Yuan ZHOU, Xue-Fang SUN, Ming ZHAO
{"title":"High Light Tolerance is Enhanced by Overexpressed PEPC in Rice Under Drought Stress","authors":"Zai-Song DING, Bao-Yuan ZHOU, Xue-Fang SUN, Ming ZHAO","doi":"10.1016/S1875-2780(11)60106-5","DOIUrl":"10.1016/S1875-2780(11)60106-5","url":null,"abstract":"<div><p>Overexpression of phosphoenolpyruvate carboxylase (PEPC) gene in transgenic rice (<em>Oryza sativa</em> L.) may alleviate inhibition on photosynthesis under drought stress condition. In the present research, photosynthetic light curve, chlorophyll α fluorescence parameters, pigment content, and reactive oxygen metabolism were studied in 2 PEPC transgenic rice lines under drought stress at flowering stage. The results showed that under drought stress, especially severely drought stress, net photosynthetic rate decreased dramatically under high photosynthetic active radiation (PAR) in untransformed wild type rice, while maintained unchanging in the PEPC transgenic lines under high PAR higher than 1200 μmol m<sup>−2</sup> s<sup>−1</sup>. The photochemistry activities (<em>F</em><sub>v</sub>/<em>F</em><sub>m</sub>, ϕ<sub>PSII</sub>, and <em>q</em><sub>P</sub>) decreased slightly under drought stress in both PEPC transgenic lines. These results indicated that PEPC enhanced the photoinhibition tolerance of rice under drought stress. The increased zeaxanthin content in leaves of the PEPC transgenic rice lines dispersed more light energy as heat under drought stress, thus decreased the producing rate in photosystem II. At the same time, the activities of superoxide dismutase, peroxidase, and catalase were higher in the PEPC transgenic rice plants than the untransformed wild type under drought stress. These enzymes could effectively diminish the reactive oxygen species and reduce the membrane lipid peroxidation.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 2","pages":"Pages 285-292"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60106-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Variation and Distribution of Vitamin E and Composition in Seeds Among Different Rice Varieties","authors":"Gui-Yun ZHANG , Ru-Ru LIU , Peng ZHANG , Yong XU , Jiang ZHU , Ming-Hong GU , Guo-Hua LIANG , Qiao-Quan LIU","doi":"10.1016/S1875-2780(11)60098-9","DOIUrl":"10.1016/S1875-2780(11)60098-9","url":null,"abstract":"<div><p>Vitamin E is an essential nutrient that must be acquired regularly from dietary sources. It is a group of compounds consisting of tocotrienols and tocopherols. The structure of tocotrienols differs from that of tocopherols by the presence of 3 trans-double bonds in the hydrocarbon chain. In this study, a reverse-phase (RP) HPLC method was used to simultaneously measure the contents of either tocopherols or tocotrienols in brown rice, and to compare these parameters among 18 <em>indica</em> and 16 <em>japonica</em> rice (<em>Oryza sativa</em> L.) varieties. The results showed that the proportion of these vitamin E isomers differed substantially between <em>indica</em> and <em>japonica</em> rice varieties. The mean contents of vitamin E and total tocopherol were significantly higher (<em>P</em> < 0.01) in <em>japonica</em> rice than in <em>indica</em> rice, while the total tocotrienol content showed no difference between <em>indica</em> and <em>japonica</em> rice. The principal isomer composition of vitamin E was also different between the 2 subspecies. For example, γ-tocotrienol was the predominant component in <em>indica</em> rice, while the most abundant isomer was α-tocopherol in <em>japonica</em> rice. The ratio of total tocotrienols to tocopherols was also significantly higher (<em>P</em> < 0.01) in <em>indica</em> (1.61) than in <em>japonica</em> rice (0.95). Pearson's correlation analysis demonstrated that the content of α-tocopherol was positively correlated to that of α-tocotrienol in rice. The same relationship was observed for γ-tocopherol and γ-tocotrienol, but there was a negative correlation between α- and γ-isomers. Taken together, the results provide valuable information for studying the vitamin E metabolism or nutritional improvement in rice.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 1","pages":"Pages 55-61"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60098-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification and Characterization of a Novel Fiber Mutant from Transgenic Progeny in Cotton","authors":"Rui ZHANG, Fen-Ni LÜ, Hai-Hai WANG, Wang-Zhen GUO","doi":"10.1016/S1875-2780(11)60096-5","DOIUrl":"10.1016/S1875-2780(11)60096-5","url":null,"abstract":"<div><p>Fiber mutants are important materials in genetic and functional research in cotton (<em>Gossypium hirsutum</em> L.). A linted-fuzzless fiber mutant, designated CM mutant, was found in transgenic cotton by <em>Agrobacterium</em>-mediated transformation method. The pure line of this mutant was obtained in T<sub>3</sub> generation. PCR analysis showed that the mutated trait had no relationship with T-DNA insertion; however, point mutation during tissue culture was deduced to be responsible for the mutated trait. Analyses of inheritance and allelic tests were conducted by crossing CM mutant with TM-1, Junhai 1, and a series of fiber developmental mutants such as XinFLM, N1N1, n2n2, and T586 with linted-fuzzless fiber and XinWX, XZ142WX, SL-7-1, and MD17 with lintless-fuzzless fiber, respectively. Among the above 10 combinations, the F<sub>1</sub> plants were fuzzless, and all the F<sub>2</sub> generations of CM × TM-1 and CM × Junhai 1 showed the segregation ratio of 3:1 of linted-fuzzless to linted-fuzzed phenotypes. Based on the genetic analysis, we indicated that there was one dominantly different locus between the mutant and TM-1 or Junhai 1. Allelic tests and gene mapping showed that the fuzzless gene of the mutant was allelic to <em>N1</em>, dominantly controlling naked-seed trait. The scanning electron microscopy observation was conducted to show the development of fiber cell initials in CM mutant during early developmental stages (0–3 d post anthesis). Like N1N1 mutant, CM mutant delayed fiber cell formation and elongation under the control of mutated gene. Compared to N1N1, CM mutant exhibited significant higher lint percentage of CM and significant lower 100-seed weight. The mutated gene in CM is speculated to be one of the multiple alleles of dominant naked-seed <em>N1</em> gene.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 1","pages":"Pages 36-42"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60096-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rong-Ping CHEN , Lie LIU , Xiu-Qing WAN , En-Jian QIU , Chun-Jun WANG , Bao-Gang SONG , Pei-Qiang YAN , Tie-Zhao YANG
{"title":"cDNA-AFLP Analysis of Differentially Expressed Genes in Tobacco Infected by Tobacco Mosaic Virus","authors":"Rong-Ping CHEN , Lie LIU , Xiu-Qing WAN , En-Jian QIU , Chun-Jun WANG , Bao-Gang SONG , Pei-Qiang YAN , Tie-Zhao YANG","doi":"10.1016/S1875-2780(11)60099-0","DOIUrl":"10.1016/S1875-2780(11)60099-0","url":null,"abstract":"<div><p>Longjiang 925 is a flue-cured tobacco variety with high resistance to <em>Tobacco mosaic virus</em> (TMV). In this study, the cDNA extracted from the TMV-inoculated leaves of Longjiang 925 was amplified with 240 pairs of primers. Approximately 9500 gene transcript fragments were obtained, in which 12 inducible expressed gene fragments were selected by cloning and sequencing. The inducible fragments functions involved in the nucleic acid metabolism, protein synthesis and modulation, energy metabolism, stress responding, intracellular transport, and metabolism of carbohydrates. The function of a differentially expressed gene sequence, TIF2, was validated using real-time PCR with the samples collected at 0, 12, 24, 48, and 72 h post inoculation. The result indicated that TIF2 was related to TMV-resistance. Both 5′- and 3′-rapid amplification of cDNA ends (RACE) were preformed using TIF2 sequence, and the full-length cDNA sequence was 875 bp in length, containing a conjecturable coding region from 101 bp to 613 bp and encoding 170 amino acids. Analyses of Blastn and Blastp showed that this gene was probably a novel gene related to TMV resistance in tobacco.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 1","pages":"Pages 62-70"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60099-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Establishment and Application of Multiplex PCR System Based on Molecular Markers for Glutenin Subunit Genes (Locus) in Wheat","authors":"Qiang LIANG, Xiao-Ke ZHANG, Qian WEI, Xiao-Long WANG, Jing ZHANG, Dao-Jie SUN, Xiao-Jie FU, Bai-Xing WU, Hong-Bo NIE","doi":"10.1016/S1875-2780(11)60050-3","DOIUrl":"10.1016/S1875-2780(11)60050-3","url":null,"abstract":"<div><p>Wheat strong-gluten quality is closely correlated with combinations of high-molecular-weight glutenin subunits (HMW-GS) and low-molecular-weight glutenin subunits (LMW-GS). A multiplex PCR system was established to evaluate the loci for HMW-GS and LMW-GS in wheat (<em>Triticum aetivum</em> L.). The multiplex PCR system confered molecular markers for <em>Ax1/Ax2*, Bx7<sup>OE</sup>, Dx5, Glu-A3d, Glu-B3i</em> genes, and <em>Glu-B3</em> locus, and proved to be effective and stable to amplify specific bands on target loci in 12 wheat cultivars with known gene/locus combinaitons. Using this multiplex PCR system, 62 major cultivars from Shaanxi Province, China were evaluated. The results showed that the frequencies were 56.5% for <em>Ax1/Ax2*</em>, 9.6% for <em>Dx5</em>, 33.9% for <em>Glu-A3d</em>, 1.6% for <em>Glu-B3i</em>, and 64.4% for <em>Glu-B3</em>, whereas gene <em>Bx7<sup>OE</sup></em> was not detected. Most cultivars carried 2 genes (locus) with the frequency of 48.3%, followed by cultivars carrying a single gene or locus (33.9%). The frequency of cultivars carrying 3- or 4-gene (locus) combinations was 11.3%. The remaining cultivars (6.5%) were free of any elite gene (locus). Therefore, the frequency of combination of multiple strong-gluten subunit genes (locus) was low in the cultivars from Shaanxi Province, which could be improved with introduced germlasm. The multiplex PCR system developed may serve as a rapid and efficient method to select breeding materials carrying multiple genes (locus) associated with strong-gluten subunit.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"37 11","pages":"Pages 1942-1948"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60050-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56941907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guang-Zhou DING , Jing HOU , Li CHEN , Feng-Ming MA , Lian-Jiang CHEN
{"title":"Cloning of nia Gene and Differential Gene Expressions Induced by Nitrate and Ammonium Nitrogen in Sugar Beet (Beta vulgaris L.)","authors":"Guang-Zhou DING , Jing HOU , Li CHEN , Feng-Ming MA , Lian-Jiang CHEN","doi":"10.1016/S1875-2780(11)60051-5","DOIUrl":"10.1016/S1875-2780(11)60051-5","url":null,"abstract":"<div><p>Gene <em>nia</em> plays an important role in regulation of nitrogen metabolism in plants. This gene has been cloned in many crops except for sugar beet. In this study, a cDNA clone associated with nitrate reduction was isolated from a diploid species of sugar beet (<em>Beta vulgaris</em> L.) using homologous cloning technique. A novel full-length cDNA (GenBank accession number EU163265), termed <em>nia</em>, was obtained using rapid amplification of cDNA ends (RACE). The full length of <em>nia</em> gene is 3247 bp. The open reading frame of this gene is 2718 bp in length, which encodes 905 amino acids with the theoretical molecular weight of 102 kD and the isoelectric point of 6.12. Southern blotting results proved that <em>nia</em> presents in low copies in Ty7, and the clone obtained belongs to NADH-NR genes. The <em>nia</em> transcripts under nitrate nitrogen (NO<sub>3</sub>-N) and ammonium nitrogen (NH<sub>4</sub>-N) treatments were detected through semiquantitative PCR analysis, and the efficiency of mRNA synthesis from each sample was estimated via quantitative PCR of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In the NH<sub>4</sub>-N treatment, <em>nia</em> was up-regulated with low concentration of NH<sub>4</sub><sup>+</sup>, but inhibited by high concentration of NH<sub>4</sub><sup>+</sup>. In the NO<sub>3</sub>-N treatment, the <em>nia</em> expression level was enhanced with the increase of NO<sub>3</sub><sup>(</sup> concentration. When exposed to 30 mmol L<sup>(1</sup> KNO<sub>3</sub>, <em>nia</em> exhibited the highest expression 4 h after treatment and a significantly reduction 6 h after treatment.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"37 11","pages":"Pages 1949-1955"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60051-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hong-Xia YUAN , Fu-Xia ZHANG , Jia-Jia ZHANG , Xing-Song HOU , Hong-Jie LI , Hong-Lian LI
{"title":"Resistance of CIMMYT Wheat Germplasm to Heterodera filipjevi Xuchang Population from Henan Province, China","authors":"Hong-Xia YUAN , Fu-Xia ZHANG , Jia-Jia ZHANG , Xing-Song HOU , Hong-Jie LI , Hong-Lian LI","doi":"10.1016/S1875-2780(11)60052-7","DOIUrl":"10.1016/S1875-2780(11)60052-7","url":null,"abstract":"<div><p>The cereal cyst nematode (CCN) of wheat has become a severe disease in recent years. <em>Heterodera filipjevi</em> is a new pathogenic nematode in China. For screening resistant resources to <em>H. filipjevi</em>, the resistance of 75 wheat cultivars or lines from the International Maize and Wheat Improvement Center (CIMMYT) was evaluated in greenhouse and field conditions using relative resistance index (RRI) and Pf/Pi ratios, with the nematode from Xuchang population, Henan Province, China. No cultivar was immune to <em>H. filipjevi</em>. However, line 6R(6D) was highly resistant in both test conditions. Lines CROC_1/AE.SQUARROSA(224)//OPATA*1, CROC_1/AE.SQUARROSA (224)//OPATA*2, MACKELLER, CPI 133842, CPI 133814, and TRIDENT were moderately resistant to <em>H. filipjevi</em> in the greenhouse test. In the field test, CPI 133842, CPI 133814, DURATI, and TURCAN#39 showed a high resistant reaction with RRI values ranging from 0.90 to 0.96. Fourteen lines including ID-2150, BAXTER, and MACKELLER were moderately resistant to <em>H. filipjevi</em>. The disease severity was heavier in the greenhouse test than in the field test, and resistance identification in the greenhouse is much easier and reliable. The results indicated that relative resistance index (RRI) is an efficient index for evaluating the resistance of wheat cultivars to CCN.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"37 11","pages":"Pages 1956-1966"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60052-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yong ZHANG , Shi-Zhao LI , Zhen-Lu WU , Wen-Xiong YANG , Ya-Xiong YU , Xian-Chun XIA , Zhong-Hu HE , De-Hui ZHAO
{"title":"Contribution of CIMMYT Wheat Germplasm to Genetic Improvement of Grain Yield in Spring Wheat of Sichuan, Yunnan, Gansu, and Xinjiang Provinces","authors":"Yong ZHANG , Shi-Zhao LI , Zhen-Lu WU , Wen-Xiong YANG , Ya-Xiong YU , Xian-Chun XIA , Zhong-Hu HE , De-Hui ZHAO","doi":"10.1016/S1875-2780(11)60047-3","DOIUrl":"10.1016/S1875-2780(11)60047-3","url":null,"abstract":"<div><p>Information on advances in wheat (<em>Triticum aestivum</em> L.) productivity is essential for genetic improvement on yield potential. Four yield potential trials with totally 59 leading cultivars from Sichuan, Yunnan, Gansu, and Xinjiang, China were conducted using a randomized complete block design with 3 replications under controlled environments in 2 successive cropping seasons from 2007 to 2009. The experimental sites were located in Chengdu in Sichuan, Lijiang in Yunnan, Wuwei in Gansu, and Changji in Xinjiang. Molecular markers were used to detect the presence of dwarfing genes and 1B/1R translocation. The results indicated that the annual genetic gains in yield were 0.73% in Sichuan, 0.34% in Yunnan, 0.58% in Gansu, and 1.43% in Xinjiang. There was no obvious trend of yield component improvement for yield increase in Sichuan; while reduced spikes per square meter and increased kernels per spike were the main factor for yield increase in Yunnan; increased kernels per spike were the main factor for yield increase in Gansu; and increased kernel weight of main spike and harvest index were the main factor for yield increase in Xinjiang, together with the contribution from reduced plant height and earlier maturity. It also indicated that the dwarfing genes <em>Rht-B1b</em> and <em>Rht-D1b</em> were all from International Maize and Wheat Improvement Center (CIMMYT) lines, and the significant progresses of genetic gain in yield in the 4 provinces were mainly due to the direct and indirect use of CIMMYT germplasm. Stripe rust resistance was the main contribution of CIMMYT germplasm in Sichuan and Yunnan; while CIMMYT germplasm contributed to high yield potential with high kernel number per spike, short plant height, and wide adaptability in Xinjiang and Gansu.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"37 10","pages":"Pages 1752-1762"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60047-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56941665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nitrogen Accumulation and Distribution in Rapeseed with Different Nitrogen Utilization Efficiencies for Grain Production","authors":"Qing-Song ZUO, Yun-Long GE, Liu Rong, Yin Cui-Yan, Tang Yao, Guang YANG, Suo-Hu LENG","doi":"10.1016/S1875-2780(11)60049-7","DOIUrl":"10.1016/S1875-2780(11)60049-7","url":null,"abstract":"<div><p>The objectives of this study were to understand the characteristics of nitrogen accumulation and partition in different types ofrapeseed (Brassica napus. L) and explain the mechanism of nitrogen efficiency for seed production. A total of 98 conventional rapeseed varieties in 6 types of nitrogen use efficiency for grain production (NUEg) were planted under N0 (pure nitrogen 0 kg ha<sup>−1</sup>) and N1 (pure nitrogen 150 kg ha<sup>−1</sup>) conditions in 2007 and 2008. The dry matter weights and nitrogen contents of different parts of plants at ripening stage were determined. Significant difference of NUEg was observed among varieties, and seed yield of rapeseed was increased with NUEg. NUEg had no significant correlation with total nitrogen accumulation, but was negatively correlated with the ratios of nitrogen distribution in stem (<em>r</em> = −0.5941, <em>P</em> < 0.01 in treatment N0; and <em>r</em> = −0.4141, <em>P</em> < 0.01 in treatment N1) and shell (<em>r</em> = −0.6007, <em>P</em> < 0.01 in N0; and <em>r</em> = −0.5374, <em>P</em> < 0.01 in N1) and positively correlated with the ratio of nitrogen accumulation in seed (<em>r</em> = 0.7954, <em>P</em> < 0.01 in N0; and <em>r</em> = 0.7239, <em>P</em> < 0.01 in N1). Besides, NUEg had significant correlation with total number of seeds (<em>r</em> = 0.5945, <em>P</em> < 0.01 in N0; and <em>r</em> = 0.5412, <em>P</em> < 0.01 in N1). Both NUEg and total amount of nitrogen accumulation had significant impacts on rapeseed yield. Therefore, rapeseed variety should be developed with high levels of nitrogen accumulation and translocation into seeds.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"37 10","pages":"Pages 1852-1859"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60049-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56941840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hui-Min WU, Li-Yu HUANG, Ya-Jiao PAN, Peng JIN, Bin-Ying FU
{"title":"Function of Gene OsASIE1 in Response to Abiotic Stress in Rice","authors":"Hui-Min WU, Li-Yu HUANG, Ya-Jiao PAN, Peng JIN, Bin-Ying FU","doi":"10.1016/S1875-2780(11)60048-5","DOIUrl":"10.1016/S1875-2780(11)60048-5","url":null,"abstract":"<div><p>AP2/EREBP transcription factors play an important role in plant development, hormone response, and responses to biotic and abiotic stress. <em>OsASIE1</em>, a member of the EREBP subfamily of AP2/EREBP transcription factors in rice (<em>Oryza sativa</em> L.), is determined to be involved in abiotic stress response. <em>OsASIE1</em> expression was induced by drought and salt stresses. In addition, an electrophoresis mobility shift assay (EMSA) revealed that the AP2 domain of the <em>OsASIE1</em> protein can bind to both DRE (dehydration-responsive element) and GCC box (ethylene response element, ERE) <em>in vitro</em>. All these results indicate that <em>OsASIE1</em> may participate in abiotic stress response by regulating the expression of downstream genes with DRE and GCC box binding. Overexpression of <em>OsASIE1</em> in transgenic rice plants might be used to improve tolerance to salt stress.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"37 10","pages":"Pages 1771-1778"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60048-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56941538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}