Acta Agronomica Sinica最新文献

{"title":"Cloning and Expression Analysis of Lysophosphatidic Acid Acyltransferase (LPAT) Encoding Gene in Peanut","authors":"Si-Long CHEN ,&nbsp;Jia-Quan HUANG ,&nbsp;Yong LEI ,&nbsp;Xiao-Ping REN ,&nbsp;Qi-Gen WEN ,&nbsp;Yu-Ning CHEN ,&nbsp;Hui-Fang JIANG ,&nbsp;Li-Ying YAN ,&nbsp;Bo-Shou LIAO","doi":"10.1016/S1875-2780(11)60104-1","DOIUrl":"10.1016/S1875-2780(11)60104-1","url":null,"abstract":"<div><p>Lysophosphatidic acid acyltransferase (LPAT) is one of the key enzymes in biosynthesis pathway of triacylglycerol (TAG) in plant. A full-length cDNA library of peanut (<em>Arachis hypogaea</em> L.) was constructed from seed by means of a large number of sequences of expressed sequence tag (EST) and gene functional annotation. A lysophosphatidic acid acyltransferase gene, designated <em>AhLPAT</em>, and its genomic DNA sequence was isolated from peanut. The sequence of <em>AhLPAT</em> cDNA was 1753 bp, and its genomic sequence was 5331 bp. Bioinformatic analysis showed that <em>AhLPAT</em> was composed of 11 exons and 10 introns with typical GT-AG sequence at the splice site. A peptide of 387 amino acid residues was deduced from <em>AhLPAT</em>, with molecular weight of 43.2 kD and isoelectric point (pI) of 9.42. Conserved domain prediction indicated that AhLPAT comprised a typical conserved acyltransferase domain and a conserved lysophospholipid acyltransferase domain. The deduced amino acid had a high sequence similarity with the LPAT proteins from other species. Similarities for amino acid sequence of LPAT protein between peanut and <em>Tropaeolum majus</em>, <em>Brassica napus</em>, <em>Crambe hispanica</em> subsp. <em>abyssinica</em>, <em>Ricinus communis</em>, and <em>Arabidopsis thaliana</em> were 90%, 89%, 89%, 88%, and 87%, respectively. The phylogenetic tree suggested that AhLPAT and AtLPAT2 derived from <em>A. thaliana</em> were grouped into the same class, and both of them were endoplasmic reticulum type LPATs. The result of quantitative RT-PCR assay indicated that <em>AhLPAT</em> was ubiquitously expressed in root, stem, leaf, flower, gynophore, and seed of peanut with the highest level in gynophore and seed. The peak expression was in the period of 50–60 d after flowering. Correlation between <em>AhLPAT</em> expression and oil accumulation was significant (<em>r</em> = 0.63, <em>P</em> &lt; 0.05). These results suggest that <em>AhLPAT</em> plays an important role in peanut TAG biosynthesis.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 2","pages":"Pages 245-255"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60104-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Analysis and Mapping of a Novel Short Root Hair Gene OsSRH3 in Rice","authors":"Wo-Na DING ,&nbsp;Wei HUANG ,&nbsp;Yong-Qiang NING ,&nbsp;Shi-Hua ZHU","doi":"10.1016/S1875-2780(11)60103-X","DOIUrl":"10.1016/S1875-2780(11)60103-X","url":null,"abstract":"<div><p>Root hair is an important organ for uptaking nutrients and water in plant. A rice (<em>Oryza sativa</em> L.) mutant, <em>ossrh3</em>, with short root hairs was isolated from a T-DNA insertion mutant library of rice on Zhonghua 11 background. The elongation of root hairs in the mutant was severely impaired. Beside, some other traits were also affected, such as plant height, primary root length, lateral root length, and number of lateral roots. Genetic analysis indicated that the mutated phenotype was controlled by a single recessive nuclear gene. An F₂ population was developed by crossing the mutant <em>ossrh3</em> with an <em>indica</em> cultivar, Kasalath, to map the gene. Using simple sequence repeat (SSR) markers and newly designed sequence-tagged site (STS) markers, the target gene, <em>OsSRH3</em>, was located to a 37.7 kb region between markers S38978 and S39016 on chromosome 1. This marker interval contained 8 predicted genes.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 2","pages":"Pages 240-244"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60103-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Quantitative Trait Loci for Resistance to Brown Planthopper in Dongxiang Wild Rice (Oryza rufipogon Griff.)","authors":"De-Run HUANG,&nbsp;Jie CHEN,&nbsp;Feng-Xiang LAI,&nbsp;Guang-Jie LIU ,&nbsp;Jie-Yun ZHUANG","doi":"10.1016/S1875-2780(11)60101-6","DOIUrl":"10.1016/S1875-2780(11)60101-6","url":null,"abstract":"<div><p>The wild species in genus <em>Oryza</em> are important resources of genes resistant to brown planthopper (<em>Nilaparvata lugens</em>, BPH). The objective of this study was to identify quantitative trait loci (QTLs) in Dongxiang wild rice (<em>O. rufipogon</em> Griff.). A BC₁F₅ population and a BC₃F₃ population were developed for QTL mapping from the cross between Dongxiang wild rice and an <em>indica</em> rice cultivar, Xieqingzao B (<em>O. sativa</em> L. subsp. <em>indica</em>) as the recurrent parent. The BC₁F₅ population was infested with BPH collected from paddy fields. Two QTLs were identified, of which <em>qBph2</em> was located in the interval of RM29-RG157 on chromosome 2 and <em>qBph7</em> in the interval of RM11-RM234 on chromosome 7. The alleles from Dongxiang wild rice on <em>qBph2</em> and <em>qBph7</em>, which explained 21.8% and 67.1% of the phenotypic variations, respectively, and reduced seedling mortality by 22.2% and 43.7%. These QTLs were validated using the BC₃F₃ population infested by BPH biotypes I, II, and III. This result shows that <em>qBph2</em> confers resistance to biotypes I and II, and <em>qBph7</em> confers resistance to biotypes I and III. Both QTLs show a great potential for improving BPH resistance in rice cultivars.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 2","pages":"Pages 210-214"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60101-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inheritance of Resistance to Soybean Mosaic Virus Strains SC4 and SC8 in Soybean","authors":"Da-Gang WANG ,&nbsp;Ying MA ,&nbsp;Ning LIU ,&nbsp;Gui-Jie ZHENG ,&nbsp;Zhong-Lu YANG ,&nbsp;Yong-Qing YANG ,&nbsp;Hai-Jian ZHI","doi":"10.1016/S1875-2780(11)60100-4","DOIUrl":"10.1016/S1875-2780(11)60100-4","url":null,"abstract":"<div><p>The objective of this study was to understand the genetic and allelomorphic relationships among different soybean (<em>Glycine max</em> L. Merr.) cultivars with resistance to <em>Soybean mosaic virus</em> (SMV). Twelve resistant (R) soybean cultivars were crossed with a susceptible (S) cultivar (Nannong 1138-2, NY30, NY185, 86-4, or 8101) to determine the inheritance of resistance to SMV strain SC4 or SC8, which were predominant in the Huang-Huai-Hai Rivers Region and Yangtze Valley in China. The R parents were also crossed with each other for allelic analysis of resistance genes from different sources. The results indicated that Ji LD42, Xudou 1, and Yuejin 4 and Qihuang 1, Zhongzuo 229, and NY58 carried a dominant resistance gene for SMV strains SC4 and SC8, respectively, whereas Kefeng 1, PI 96983, Jinda 74, Fendou 56, Dabaima, and Qihuang 22 carried single dominant resistance gene for both SC4 and SC8. The allelism test of the resistance genes to SC4 showed that the resistance genes between Dabaima and Fendou 56, Kefeng 1, and Qihuang 1, between Ji LD42 and Fendou 56, and between Jinda 74 and Zhongzuo 229 were not at the same loci. The study of resistance of F₂ plants and F_2:3 families to SC8 indicated that the single dominant resistance genes in Jinda 74 and Fendou 56 shared a common locus or two closely loci. The resistance genes between Qihuang 1 and Kefeng 1 and between Dabaima and Fendou 56 were not at the same locus for SC8. These results are useful to understand the genetic and allelomorphic relationships among various sources of resistance.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 2","pages":"Pages 202-209"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60100-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Light Tolerance is Enhanced by Overexpressed PEPC in Rice Under Drought Stress","authors":"Zai-Song DING,&nbsp;Bao-Yuan ZHOU,&nbsp;Xue-Fang SUN,&nbsp;Ming ZHAO","doi":"10.1016/S1875-2780(11)60106-5","DOIUrl":"10.1016/S1875-2780(11)60106-5","url":null,"abstract":"<div><p>Overexpression of phosphoenolpyruvate carboxylase (PEPC) gene in transgenic rice (<em>Oryza sativa</em> L.) may alleviate inhibition on photosynthesis under drought stress condition. In the present research, photosynthetic light curve, chlorophyll α fluorescence parameters, pigment content, and reactive oxygen metabolism were studied in 2 PEPC transgenic rice lines under drought stress at flowering stage. The results showed that under drought stress, especially severely drought stress, net photosynthetic rate decreased dramatically under high photosynthetic active radiation (PAR) in untransformed wild type rice, while maintained unchanging in the PEPC transgenic lines under high PAR higher than 1200 μmol m⁻² s⁻¹. The photochemistry activities (<em>F</em>_v/<em>F</em>_m, ϕ_PSII, and <em>q</em>_P) decreased slightly under drought stress in both PEPC transgenic lines. These results indicated that PEPC enhanced the photoinhibition tolerance of rice under drought stress. The increased zeaxanthin content in leaves of the PEPC transgenic rice lines dispersed more light energy as heat under drought stress, thus decreased the producing rate in photosystem II. At the same time, the activities of superoxide dismutase, peroxidase, and catalase were higher in the PEPC transgenic rice plants than the untransformed wild type under drought stress. These enzymes could effectively diminish the reactive oxygen species and reduce the membrane lipid peroxidation.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 2","pages":"Pages 285-292"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60106-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency of Recurrent Selection for Combining Ability of Yield Trait in Maize Population Yuzong 5","authors":"Li-Xia KU ,&nbsp;Qing-Lei MENG ,&nbsp;Ben-Jun HOU ,&nbsp;Jia-Fu LI ,&nbsp;Hai-Jing LIU ,&nbsp;Yan-Hui CHEN","doi":"10.1016/S1875-2780(11)60102-8","DOIUrl":"10.1016/S1875-2780(11)60102-8","url":null,"abstract":"<div><p>Population improvement is a basis of germplasm enhancement and developing inbred lines in maize (<em>Zea mays</em> L.). For evaluating the combing abilities of Yuzong 5 developed populations derived from 5 cycles of recurrent selection, 30 testcrossing combinations were made using Huangzao 4, Dan 340, Zhongzong 5, Qi 319, Ye 478, and Mo17 as the female parents in 2 locations under the NCII design. The yield per plant of testcrossing combinations was enhanced by 3.57% per cycle, showing a remarkable effect of recurrent selection. Both half-sib recurrent selection (HS-RS) and half-sib reciprocal recurrent selection (HS-RRS) were effective for improving the general combining ability (GCA) of kernel yield and the GCA was improved in each cycle of the populations. After 3 cycles of improvement in Yuzong 5 by HS-RS, the GCA significantly increased from (11.63 of C₀ to 5.57 of C₃ population (<em>P</em> &lt; 0.01). After 1 cycle of improvement by HS-RRS, the GCA increased from 5.57 of C₃ to 9.75 of C₄ population. The specific combining ability (SCA) of improved Yuzong 5 × Huangzao 4 was enhanced, which indicated an approaching Reid × SiPT heterotic pattern during Yuzong 5 improvement cycle by cycle.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 2","pages":"Pages 215-222"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60102-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation and Distribution of Vitamin E and Composition in Seeds Among Different Rice Varieties","authors":"Gui-Yun ZHANG ,&nbsp;Ru-Ru LIU ,&nbsp;Peng ZHANG ,&nbsp;Yong XU ,&nbsp;Jiang ZHU ,&nbsp;Ming-Hong GU ,&nbsp;Guo-Hua LIANG ,&nbsp;Qiao-Quan LIU","doi":"10.1016/S1875-2780(11)60098-9","DOIUrl":"10.1016/S1875-2780(11)60098-9","url":null,"abstract":"<div><p>Vitamin E is an essential nutrient that must be acquired regularly from dietary sources. It is a group of compounds consisting of tocotrienols and tocopherols. The structure of tocotrienols differs from that of tocopherols by the presence of 3 trans-double bonds in the hydrocarbon chain. In this study, a reverse-phase (RP) HPLC method was used to simultaneously measure the contents of either tocopherols or tocotrienols in brown rice, and to compare these parameters among 18 <em>indica</em> and 16 <em>japonica</em> rice (<em>Oryza sativa</em> L.) varieties. The results showed that the proportion of these vitamin E isomers differed substantially between <em>indica</em> and <em>japonica</em> rice varieties. The mean contents of vitamin E and total tocopherol were significantly higher (<em>P</em> &lt; 0.01) in <em>japonica</em> rice than in <em>indica</em> rice, while the total tocotrienol content showed no difference between <em>indica</em> and <em>japonica</em> rice. The principal isomer composition of vitamin E was also different between the 2 subspecies. For example, γ-tocotrienol was the predominant component in <em>indica</em> rice, while the most abundant isomer was α-tocopherol in <em>japonica</em> rice. The ratio of total tocotrienols to tocopherols was also significantly higher (<em>P</em> &lt; 0.01) in <em>indica</em> (1.61) than in <em>japonica</em> rice (0.95). Pearson's correlation analysis demonstrated that the content of α-tocopherol was positively correlated to that of α-tocotrienol in rice. The same relationship was observed for γ-tocopherol and γ-tocotrienol, but there was a negative correlation between α- and γ-isomers. Taken together, the results provide valuable information for studying the vitamin E metabolism or nutritional improvement in rice.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 1","pages":"Pages 55-61"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60098-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Characterization of a Novel Fiber Mutant from Transgenic Progeny in Cotton","authors":"Rui ZHANG,&nbsp;Fen-Ni LÜ,&nbsp;Hai-Hai WANG,&nbsp;Wang-Zhen GUO","doi":"10.1016/S1875-2780(11)60096-5","DOIUrl":"10.1016/S1875-2780(11)60096-5","url":null,"abstract":"<div><p>Fiber mutants are important materials in genetic and functional research in cotton (<em>Gossypium hirsutum</em> L.). A linted-fuzzless fiber mutant, designated CM mutant, was found in transgenic cotton by <em>Agrobacterium</em>-mediated transformation method. The pure line of this mutant was obtained in T₃ generation. PCR analysis showed that the mutated trait had no relationship with T-DNA insertion; however, point mutation during tissue culture was deduced to be responsible for the mutated trait. Analyses of inheritance and allelic tests were conducted by crossing CM mutant with TM-1, Junhai 1, and a series of fiber developmental mutants such as XinFLM, N1N1, n2n2, and T586 with linted-fuzzless fiber and XinWX, XZ142WX, SL-7-1, and MD17 with lintless-fuzzless fiber, respectively. Among the above 10 combinations, the F₁ plants were fuzzless, and all the F₂ generations of CM × TM-1 and CM × Junhai 1 showed the segregation ratio of 3:1 of linted-fuzzless to linted-fuzzed phenotypes. Based on the genetic analysis, we indicated that there was one dominantly different locus between the mutant and TM-1 or Junhai 1. Allelic tests and gene mapping showed that the fuzzless gene of the mutant was allelic to <em>N1</em>, dominantly controlling naked-seed trait. The scanning electron microscopy observation was conducted to show the development of fiber cell initials in CM mutant during early developmental stages (0–3 d post anthesis). Like N1N1 mutant, CM mutant delayed fiber cell formation and elongation under the control of mutated gene. Compared to N1N1, CM mutant exhibited significant higher lint percentage of CM and significant lower 100-seed weight. The mutated gene in CM is speculated to be one of the multiple alleles of dominant naked-seed <em>N1</em> gene.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 1","pages":"Pages 36-42"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60096-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Affected BC1F1 Size for Development of Genome-Wide Introgression Lines","authors":"Long YAN ,&nbsp;Hui-Yong LIU ,&nbsp;Ying-Hui LI ,&nbsp;Meng-Chen ZHANG ,&nbsp;Li-Juan QIU","doi":"10.1016/S1875-2780(11)60097-7","DOIUrl":"10.1016/S1875-2780(11)60097-7","url":null,"abstract":"<div><p>Introgression lines are important genetic materials for genetics study and breeding. Development of those lines involves cross and backcross processes between recipient and donor parents. The population size of BC₁F₁ is a critical parameter for fully covering donor genome and successfully obtaining desired introgression lines. However, the minimum sufficient number of BC₁F₁ plants is unknown for each species and cannot be obtained experimentally. A computer program was developed by simulating the recombination process during meiosis to define the ideal BC₁F₁ population size. The reliability of the program was confirmed by mathematics and experimental data. Three factors including linkage group number, linkage group length, and gene density were analyzed, and all of them had positive relation with the size of BC₁F₁ population. The population size increased from 6.06 to 9.49 when the linkage number increased from 5 to 40. The population size was 7.14 when the linkage group length was 80 cM, while it became 8.64 when the length was 200 cM. The population size was 7.65 with the density of 20 cM per gene and 8.22 with 10 cM per gene. The BC₁F₁ population sizes of rice (<em>Oryza sativa</em> L.), wheat (<em>Triticum aestivum</em> L.), maize (<em>Zea mays</em> L.), and soybean (<em>Glycine max</em> L. Merr.) were predicted to be 12, 13, 14–15, and 13, respectively, by the program with 95% confidential level.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 1","pages":"Pages 50-54"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60097-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"cDNA-AFLP Analysis of Differentially Expressed Genes in Tobacco Infected by Tobacco Mosaic Virus","authors":"Rong-Ping CHEN ,&nbsp;Lie LIU ,&nbsp;Xiu-Qing WAN ,&nbsp;En-Jian QIU ,&nbsp;Chun-Jun WANG ,&nbsp;Bao-Gang SONG ,&nbsp;Pei-Qiang YAN ,&nbsp;Tie-Zhao YANG","doi":"10.1016/S1875-2780(11)60099-0","DOIUrl":"10.1016/S1875-2780(11)60099-0","url":null,"abstract":"<div><p>Longjiang 925 is a flue-cured tobacco variety with high resistance to <em>Tobacco mosaic virus</em> (TMV). In this study, the cDNA extracted from the TMV-inoculated leaves of Longjiang 925 was amplified with 240 pairs of primers. Approximately 9500 gene transcript fragments were obtained, in which 12 inducible expressed gene fragments were selected by cloning and sequencing. The inducible fragments functions involved in the nucleic acid metabolism, protein synthesis and modulation, energy metabolism, stress responding, intracellular transport, and metabolism of carbohydrates. The function of a differentially expressed gene sequence, TIF2, was validated using real-time PCR with the samples collected at 0, 12, 24, 48, and 72 h post inoculation. The result indicated that TIF2 was related to TMV-resistance. Both 5′- and 3′-rapid amplification of cDNA ends (RACE) were preformed using TIF2 sequence, and the full-length cDNA sequence was 875 bp in length, containing a conjecturable coding region from 101 bp to 613 bp and encoding 170 amino acids. Analyses of Blastn and Blastp showed that this gene was probably a novel gene related to TMV resistance in tobacco.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 1","pages":"Pages 62-70"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60099-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}