Acta Agronomica Sinica最新文献

{"title":"Genome-wide association analysis reveals zinc-tolerant loci of rapeseed at germination stage","authors":"Lijuan Wei, Shulin Shen, Xiaohu Huang, Guoqiang Ma, Xi-Tong Wang, Yi-Ling Yang, Huan-Dong Li, Shuxian Wang, Mei-Chen Zhu, Zhanglin Tang, Kun Lu, Li Jiana, C. Qu","doi":"10.3724/sp.j.1006.2021.04037","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2021.04037","url":null,"abstract":"400715, China Abstract: Zinc (Zn) is one of the important mircroelements, but the excessive amount application would affect plant growth and development. Genome-wide association analysis (GWAS) was performed on the relative hypocotyl length (RHL) using the 140 B. napus genotyped under zinc stress treatment (30 mg L  1 ) at germination stage by Illumina 60K SNP array, and then significant SNP locus and candidate genes were detected. In the study, the population structure analysis revealed that the 140 B. napus were classified into two subgroups, and the kinship coefficients of the 89% materials were less than 0.1, indicating the tested population had a distant relationship. GWAS analysis indicated that there were significantly 8 SNP locus correlated RNA-Seq. GO enrichment analysis indicated that the up-regulated genes mainly participated in redox reaction, ion transport, stress response, defense response and sulfur compound transport. Nineteen candidate genes response to zinc stress were identified by GWAS analysis and RNA-seq, including the genes encoding zinc finger protein (B-box type and ZFP1), glutathione transferase GSTU21, peroxidase family protein, ABC and MFS transporters, cell wall-related kinase protein, and genes encoding transcription factors (TF), such as BnaA07g27330D (MYB), BnaA02g30270D (bHLH), BnaA07g27840D (WRKY57), BnaA07g31860D (ORA47), and BnaA07g28000 (NAC). This study laid the foundation for understanding the molecular mechanism of zinc stress in B. napus .","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"11 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141205976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine mapping of a major QTL qMES20-10 associated with deep-seeding tolerance in maize and analysis of differentially expressed genes","authors":"Guoying Wang, Mengxuan Ren, Jun Zheng, Jian-hua Wang, Hong-wei Zhang","doi":"10.3724/sp.j.1006.2020.93054","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2020.93054","url":null,"abstract":"","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"138 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141209914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine mapping of a major QTL qMES20-10 associated with deep-seeding tolerance in maize and analysis of differentially expressed genes","authors":"Guoying Wang, Mengxuan Ren, Jun Zheng, Jian-hua Wang, Hong-wei Zhang","doi":"10.3724/sp.j.1006.2020.93054","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2020.93054","url":null,"abstract":"","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"137 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141209928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of methodical nitrogen-water distribution management on water and nitrogen use efficiency of rice","authors":"Ming-jin Jiang, Xiangping Guo, Yan He, Tian-Rong Yan, Yong-Jian Sun, Tengfei Lyu, Changchun Guo, Rong Hu, Yu Li, Na Li, Zhiyuan Yang, Jun Ma, Peng Ma","doi":"10.3724/sp.j.1006.2020.92027","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2020.92027","url":null,"abstract":"China Abstract: This study included three split-plot designed experiments. Experiments 1 and 2 were conducted in two fields with varied soil fertility and consistent treatment. Two rice varieties (Dexiang 4103, high NUE; Yixiang 3724, low NUE) were set as main plot. The sub-plot contained six nitrogen-water management modes (farmer’s usual management, FU; nitrogen-water coupling management, NWC; methodical nitrogen-water distribution management, MNWD; and their respective nitrogen-free controls). The main plot of Exp.3 was two high NUE varieties (Dexiang 4103, Fyou 498) and two low NUE varieties (Yixiang 3724, Chuanyou 6203); FU, NWC, and MNWD assembled the sub-plot. MNWD adopted the method of increasing frequency and re-ducing quantity, thus the nitrogen application rate was reduced by 20% compared with NWC and FU, the irrigation water amount was reduced by 20% to 25% compared with NWC, and smoothly and its ear bearing tiller percentage was higher. Compared with NWC and FU, the photo assimilation before anthesis MNWD had less, dry matter transportation before anthesis and high accumulation of assimilate after anthesis. The grain yield of MNWD was similar to that of NWC, while 8.77%–14.18% higher than that of FU. Correlation analysis showed that the dry weight of roots in 10–20 cm and 20–30 cm soil layers were significantly and positively correlated with nitrogen recovery efficiency (NRE), nitrogen agronomy efficiency (NAE), irrigation water production efficiency (IWPE) and water production efficiency (WPE). MNWD had a large amount of root system distributed in the soil layer below 10 cm, which was conducive to the improvement of water and nitrogen utilization efficiency. Compared with NWC and FU, MNWD increased NRE by 8.07%–11.99% and 20.72%–30.78%, NAE by 17.44%–27.38% and 96.47%–101.42%, IWPE by 23.34%–36.67% and 76.54%–117.38%, WPE by 8.41%–17.66% and 32.23%–65.29%, respectively.","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"6 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141226160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Quick Method for Detecting Target DNA Binding Sites of Protein","authors":"Ming ZHU ,&nbsp;Wei WEI ,&nbsp;Ming CHEN ,&nbsp;Xian-Sheng ZHANG ,&nbsp;Zhao-Shi XU ,&nbsp;Lian-Cheng LI ,&nbsp;You-Zhi MA","doi":"10.1016/S1875-2780(11)60056-4","DOIUrl":"10.1016/S1875-2780(11)60056-4","url":null,"abstract":"<div><p>DREB (dehydration responsive element binding protein) transcription factors play important roles in stress response and regulation of plant growth and development. In general, the traditional method of DNase I foot-printing is used to identify DNA binding of transcription factor. The DNA probes were labeled with isotope and then polyacrylamide gel electrophoresis was performed to separately digested labeled-DNA fragments, which had low resolution ratio and complexity in operation, and was not suitable for detecting interactions in large scales. To explore the transcriptional regulation mechanism of GmDREB3, the DNase I foot-printing method was improved. Combing with electrophoretic mobility shift assay (EMSA), DNA was labeled by fluorescence instead of isotope and the capillary electrophoresis instead of polyacrylamide gel electrophoresis was used to detect the digested DNA fragments and further identify DNA binding region of GmMYB1 in promoter of GmDREB3 gene. DNA binding site of GmMYB1 in GmDREB3 promoter was confirmed via the method of restriction enzyme digesting DNA. To further confirm binding element in GmDREB3 promoter, we used a putative DNA binding element of GmMYB1 to complete EMSA, indicating that GmMYB1 can bind target DNA element <em>in vitro</em>. In short, compared with classic DNase I foot-printing, the modified method is more rapid, accurate and reliable, and will be a high throughput method to identify interactions between proteins and target DNA sites.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"37 12","pages":"Pages 2167-2171"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60056-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction Between Two Self-Incompatible Signal Elements, EXO70A1 and ARC1","authors":"Kun YANG ,&nbsp;He-Cui ZANG ,&nbsp;Richard CONVERSE ,&nbsp;Li-Quan ZHU ,&nbsp;Yong-Jun YANG ,&nbsp;Li-Yan XUE ,&nbsp;Bing LUO ,&nbsp;Deng-Long CHANG ,&nbsp;Qi-Guo GAO ,&nbsp;Xiao-Jia WANG","doi":"10.1016/S1875-2780(11)60054-0","DOIUrl":"10.1016/S1875-2780(11)60054-0","url":null,"abstract":"<div><p>ARC1 and EXO70A1 are important signal elements of self-incompatibility in <em>Brassica</em>. To characterize the interaction of ARC1-EXO70A1 during the course of self-incompatibility, the coding sequences of <em>ARC1</em> and <em>EXO70A1</em> were cloned from <em>Brassica napus</em> L. and <em>B. oleracea</em> L. var.<em>acephala</em>. Sequence analysis showed that ARC1 consisted of 663 amino acids in <em>B. oleracea</em> and 661 amino acids in <em>B. napus</em>, with a 45-amino-acid difference between them. Sequence alignment showed 95.9% similarity, with 93.9% exact match between BoARC1 and BnARC1. Only a 6-amino-acid difference was detected between BoEXO70A1 and BnEXO70A1, with 99.4% similarity and 98.9% exact match according to further sequence alignment. The homology between EXO70A1 alleles was higher than that between ARC1 alleles. Yeast 2-hybrid results indicated that a strong interaction existed between ARC1 and EXO70A1, which could activate the expressions of 4 reporter genes (<em>ADE2</em>, <em>HIS3</em>, <em>AUR1-C</em>, and <em>MEL1</em>) in diploid yeast. However, there was very weak interaction between EXO70A1 and a 316-C-terminal-deletion mutant of ARC1 (ARC1_N), which only activated the expressions from 3 reporter genes (<em>ADE2</em>, <em>AUR1-C</em>, and <em>MEL1</em>). This indicated that the interaction interface between ARC1 and EXO70A1 might not reside within the Armadillo (ARM) repeat domains of ARC1. The N-terminal domains of ARC1 play an essential role in the interaction of ARC1 with EXO70A1. The influence of the differences in amino-acid composition between BoARC1 and BnARC1 on the interaction between ARC1-EXO70A1 was not detected with a yeast 2-hybrid system, which may indicate that the binding interface between ARC1 and EXO70A1 was not altered by sequence differences between the 2 proteins in these <em>Brassica</em> species.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"37 12","pages":"Pages 2136-2144"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60054-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Zhongliang 21","authors":"Dong-Fang MA ,&nbsp;Hai-Ge WANG ,&nbsp;Ming-Shuang TANG ,&nbsp;Xi-Li YUAN ,&nbsp;Yao-Bo BAI ,&nbsp;Xin-Li ZHOU ,&nbsp;Jian-Rong SONG ,&nbsp;Jin-Xue JING","doi":"10.1016/S1875-2780(11)60055-2","DOIUrl":"10.1016/S1875-2780(11)60055-2","url":null,"abstract":"<div><p>Wheat (<em>Triticum aestivum</em> L.) cultivar Zhongliang 21 displays resistance to 7 prevalent <em>Puccinia striiformis</em> f. sp. <em>tritici</em> (<em>Pst</em>) races from China. The resistance gene(s) carried by this cultivar was identified using molecular markers in combination with phenotypic evaluation. Seedlings of the F₁, F₂, and BC₁ generations from the cross between Zhongliang 21 (resistant) and Mingxian 169 (susceptible), as well as the parents, were inoculated with <em>Pst</em> race CYR30 in greenhouse, and the resistance was evaluated 15-16 d after inoculation. The results showed that the stripe rust resistance in Zhongliang 21 was conferred by a single dominant gene, which was designated tentatively <em>Yrzhong21</em>. Based on bulked segregant analysis, 10 simple sequence repeat (SSR) markers located on chromosome 5AL were identified to be linked to <em>Yrzhong21</em>, of which <em>Xgwm186</em> and<em>Xbarc165</em> were the closest flanking markers with genetic distances of 7.4 and 2.7 cM, respectively. In combination with analyses of chromosomal location, reactions to various pathotypes, and pedigree, <em>Yrzhong21</em> was deduced as a novel gene resistant to stripe rust. Eighteen wheat cultivars (lines) in Zhongliang series were further screened with markers <em>Xgwm186</em> and<em>Xbarc165</em>. Only 3 cultivars produced identical banding pattern to that of Zhongliang 21. This result primarily indicates that only 17% of Zhongliang cultivars (lines) might carry <em>Yrzhong21</em>. This resistance gene is promising in wheat breeding programs for strip rust resistance.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"37 12","pages":"Pages 2145-2151"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60055-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insect Pollinators in CGMS Hybrid Seed Production of Cajanus cajan","authors":"Zheng-Hong LI ,&nbsp;Ning LIANG ,&nbsp;Hong MA ,&nbsp;Kul Bhushan SAXENA ,&nbsp;Tao YANG ,&nbsp;Xiu-Xian LIU ,&nbsp;Xu-Xiao ZONG","doi":"10.1016/S1875-2780(11)60057-6","DOIUrl":"10.1016/S1875-2780(11)60057-6","url":null,"abstract":"<div><p>Pigeonpea [<em>Cajanus cajan</em> (L.) Millspaugh] is the only shrubby food legume crop with drought tolerance in the world. Insect pollinators are essential to flower pollination in cytoplasmic genetic male sterility (CGMS) pigeonpea lines, and the species, abundance, and visiting frequency of insect pollinators are the key factors for pigeonpea hybrid production. More than 46 species of insects have been reported to be flower visitors in the open field for pigeonpea production outside China, of which <em>Megachile</em> spp. are the major pollinators. In this study, the species, abundance, and visiting frequency of flower-visiting insects at flowering stage, as well as hybrid yield of pigeonpea, were investigated in the pigeonpea hybrid production field in Yuanmou County, Yunnan Province, China, using CGMS-based ICPH2671 hybrid. A total of 25 species of flower-visiting insects were detected, including 5 major pollinator species, <em>Megachile velutina</em> Sm., <em>Megachile</em> sp-5, <em>Xylocopa tenuiscapa</em> Westw., <em>Apinae</em> sp-1, and <em>Megachile</em> sp-2. At the blooming stage, the flower-visiting insects visited each primary branch at a frequency of 2.8 times per 10 min for the CGMS male sterile line, while at a frequency of 5.2 times per 10 min for the CGMS restorer line. This indicated the preference of flower-visiting insects to the flowers of restorer line. This significant difference between the male sterile and the restorer lines resulted in very similar dry seed yields of the male sterile line (383.7 g per plant) and the restorer line (357.0 g per plant). Therefore, enough pollens can been transported from the restorer line to the male sterile line by insect pollinators, even much less visiting frequency on the flowers of male sterile line compared to that of the restorer line.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"37 12","pages":"Pages 2187-2193"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60057-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Cadmium Stress on Physiological Characteristics, Pod Yield, and Kernel Quality in Peanut","authors":"Fang GAO ,&nbsp;Ying-Jie LIN ,&nbsp;Jia-Lei ZHANG ,&nbsp;Chuan-Ting YANG ,&nbsp;Feng ZHANG ,&nbsp;Xiao-Kang YANG ,&nbsp;Hua-Jian ZHAO ,&nbsp;Xiang-Dong LI","doi":"10.1016/S1875-2780(11)60058-8","DOIUrl":"10.1016/S1875-2780(11)60058-8","url":null,"abstract":"<div><p>The objectives of this study were to study the effect of cadmium (Cd) stress on peanut (<em>Arachis hypogaea</em> L.) growth and kernel yield and quality, and provide a guide for the safe production of peanut. In the pot experiments carried out in 2009 and 2010, 2 cultivars, Yuhua 15 and XB023, were cultured under light (1.0 mg kg⁻¹), medium (2.5 mg kg⁻¹), heavy (7.5 mg kg⁻¹), and high (15.0 mg kg⁻¹) Cd stress. No Cd stresses treatment was used as the control. The vegetative growth of peanut plants were stimulated under light and medium Cd conditions, but depressed under heavy and high Cd conditions. The chlorophyll contents and photosynthetic rates of leaves of Yuhua 15 decreased with the increase of Cd concentrations. The chlorophyll contents of XB023 were increased under light and medium Cd stresses and decreased under heavy and high Cd stresses, while photosynthetic rates of leaves of XB023 decreased with the increase of Cd concentrations. The sensitivities of yield to Cd stress were different in the 2 cultivars. Yuhua 15 were more sensitive to Cd stress than XB023, because yield per pot, kernel weight per pot, kernel rate per pod, and pod number per plant in Yuhua 15 were significantly reduced under medium or higher Cd concentrations, while those in XB023 were significantly reduced under heavy and high Cd concentrations. The influence of Cd stress on seed quality also had genotypic interaction. In Yuhua 15, Cd stress increased the content of soluble sugar and decreased the contents of protein and oil, as well as the ratio of oleic-acid-to-linoleic-acid (O/L); in XB023, Cd stress decreased the contents of soluble sugar and oil and O/L ratio, and light and medium Cd stresses increased the contents of protein and its components of Lys and Thr in peanut kernels. In both cultivars, the Cd contents in kernel were higher than that in the control. Yuhua 15 had higher Cd content in plant and lower Cd content in kernel than XB 023.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"37 12","pages":"Pages 2269-2277"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60058-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and Expression Analysis of Lysophosphatidic Acid Acyltransferase (LPAT) Encoding Gene in Peanut","authors":"Si-Long CHEN ,&nbsp;Jia-Quan HUANG ,&nbsp;Yong LEI ,&nbsp;Xiao-Ping REN ,&nbsp;Qi-Gen WEN ,&nbsp;Yu-Ning CHEN ,&nbsp;Hui-Fang JIANG ,&nbsp;Li-Ying YAN ,&nbsp;Bo-Shou LIAO","doi":"10.1016/S1875-2780(11)60104-1","DOIUrl":"10.1016/S1875-2780(11)60104-1","url":null,"abstract":"<div><p>Lysophosphatidic acid acyltransferase (LPAT) is one of the key enzymes in biosynthesis pathway of triacylglycerol (TAG) in plant. A full-length cDNA library of peanut (<em>Arachis hypogaea</em> L.) was constructed from seed by means of a large number of sequences of expressed sequence tag (EST) and gene functional annotation. A lysophosphatidic acid acyltransferase gene, designated <em>AhLPAT</em>, and its genomic DNA sequence was isolated from peanut. The sequence of <em>AhLPAT</em> cDNA was 1753 bp, and its genomic sequence was 5331 bp. Bioinformatic analysis showed that <em>AhLPAT</em> was composed of 11 exons and 10 introns with typical GT-AG sequence at the splice site. A peptide of 387 amino acid residues was deduced from <em>AhLPAT</em>, with molecular weight of 43.2 kD and isoelectric point (pI) of 9.42. Conserved domain prediction indicated that AhLPAT comprised a typical conserved acyltransferase domain and a conserved lysophospholipid acyltransferase domain. The deduced amino acid had a high sequence similarity with the LPAT proteins from other species. Similarities for amino acid sequence of LPAT protein between peanut and <em>Tropaeolum majus</em>, <em>Brassica napus</em>, <em>Crambe hispanica</em> subsp. <em>abyssinica</em>, <em>Ricinus communis</em>, and <em>Arabidopsis thaliana</em> were 90%, 89%, 89%, 88%, and 87%, respectively. The phylogenetic tree suggested that AhLPAT and AtLPAT2 derived from <em>A. thaliana</em> were grouped into the same class, and both of them were endoplasmic reticulum type LPATs. The result of quantitative RT-PCR assay indicated that <em>AhLPAT</em> was ubiquitously expressed in root, stem, leaf, flower, gynophore, and seed of peanut with the highest level in gynophore and seed. The peak expression was in the period of 50–60 d after flowering. Correlation between <em>AhLPAT</em> expression and oil accumulation was significant (<em>r</em> = 0.63, <em>P</em> &lt; 0.05). These results suggest that <em>AhLPAT</em> plays an important role in peanut TAG biosynthesis.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"38 2","pages":"Pages 245-255"},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(11)60104-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56942956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}