Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology最新文献_第5页

Sorbitol increases the growth inhibition of xylitol on Strep. mutans OMZ 176. 山梨醇增加木糖醇对链球菌的生长抑制作用。变形omz176。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-08-01 DOI: 10.1111/j.1699-0463.1986.tb03046.x

S Assev, G Rølla

{"title":"Sorbitol increases the growth inhibition of xylitol on Strep. mutans OMZ 176.","authors":"S Assev, G Rølla","doi":"10.1111/j.1699-0463.1986.tb03046.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03046.x","url":null,"abstract":"It was observed in a previous study that the growth of Streptococcus mutans strain OMZ 176 on sorbitol was inhibited by xylitol. The aim of the present study was to investigate the mechanisms involved in this inhibition. It was shown that the uptake of 14C-sorbitol was delayed when the cells had been pre-exposed to xylitol, and that the only labelled substance found intracellularly was sorbitol; no further metabolization occurred. This is in contrast with untreated normal cells, where sorbitol is taken up by a specific phosphotransferase system (pts). The 14C-xylitol metabolism of the cells was qualitatively unchanged in the presence of sorbitol; an intracellular accumulation of 14C-xylitol-phosphate (xylitol-P) and 14C-xylulose-phosphate (xylulose-P) was observed. However, a reduced uptake of xylitol was observed in the presence of sorbitol. Xylitol thus appears to change the pathway by which sorbitol is taken up by the cells. An inducible permease may replace the normal sorbitol pts when xylitol is present. No further metabolization of this intracellular sorbitol seemed to occur in the resting cell suspensions. It was furthermore observed that the presence of sorbitol enhanced the inhibitory potential of xylitol. The accumulation of intracellular sorbitol coincided with markedly increased xylulose-P/xylitol-P ratio. It may be speculated that, if xylulose-P were the major inhibitor of the glycolysis instead of xylitol-P, as previously assumed, an increased concentration of xylulose-P induced by sorbitol could explain that sorbitol enhances the inhibition potential of xylitol. It is not evident, however, how intracellular sorbitol could affect the xylulose-P/xylitol-P ratio.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 4","pages":"231-7"},"PeriodicalIF":0.0,"publicationDate":"1986-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03046.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14869530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The beta-lactamase stability and in vitro activity of cefotetan; a comparison with 8 other beta-lactam antibiotics and gentamicin. 头孢替坦β -内酰胺酶稳定性及体外活性研究其他8种β -内酰胺类抗生素与庆大霉素的比较。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-08-01 DOI: 10.1111/j.1699-0463.1986.tb03049.x

H Friis, J Prag, E Togsverd, M W Benzon

引用次数: 1

Reinvestigation and reclassification of a collection of 56 human isolates of Pasteurellaceae. 56株巴氏杆菌人分离株的再调查和重新分类。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-08-01 DOI: 10.1111/j.1699-0463.1986.tb03044.x

M Bisgaard, E Falsen

引用次数: 11

A staphylococcal alpha-toxin fragment. Its characterization and use for mapping biologically-active regions of alpha-toxin. 葡萄球菌α毒素片段。它的特性和用于绘制α毒素的生物活性区域。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-08-01

L Blomqvist, M Thelestam

{"title":"A staphylococcal alpha-toxin fragment. Its characterization and use for mapping biologically-active regions of alpha-toxin.","authors":"L Blomqvist, M Thelestam","doi":"","DOIUrl":"","url":null,"abstract":"A fragment (alpha-13) of Staphylococcal alpha-toxin was compared with intact alpha-toxin as regards biochemical and biological properties, and the resulting information was used for mapping biologically-active regions of alpha-toxin. The alpha-13 fragment had an apparent Mr of 18,500, judged by sodium dodecylsulphate polyacrylamide gel electrophoresis. However, it showed the same relative mobility as alpha-toxin when subjected to gel filtration on Biogel P60, high pressure liquid chromatography or electrophoresis on polyacrylamide gradient gel. The fragment had roughly the same specific hemolytic activity as intact alpha-toxin. In contrast to intact alpha-toxin, the fragment was neither membrane-damaging to mouse adrenocortical (Y 1) tumour cells nor lethal to mice. However, a short treatment of Y 1 cells with the fragment completely blocked intoxication by subsequently-added alpha-toxin. Likewise, the lethal effect of alpha-toxin was inhibited when the fragment was injected prior to the toxin. Thus, the fragment had lost the active region(s) responsible for Y 1 cell intoxication and lethality, while the region(s) for binding to these targets, as well as the region responsible for hemolysis, were retained. On the basis of these findings and previous reports concerning tryptic fragments of alpha-toxin, a hypothetical map of the different biologically-active regions of alpha-toxin was established.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 4","pages":"277-83"},"PeriodicalIF":0.0,"publicationDate":"1986-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14869534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunoadsorbent-purified antibodies in the study of antigenic relatedness of outer membrane proteins of enteric bacilli. 免疫吸附纯化抗体在肠杆菌外膜蛋白抗原相关性研究中的应用。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-08-01 DOI: 10.1111/j.1699-0463.1986.tb03050.x

A Z Henriksen, J A Maeland

{"title":"Immunoadsorbent-purified antibodies in the study of antigenic relatedness of outer membrane proteins of enteric bacilli.","authors":"A Z Henriksen, J A Maeland","doi":"10.1111/j.1699-0463.1986.tb03050.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03050.x","url":null,"abstract":"Immunoadsorbent chromatography was used for purification of antibodies to E. coli 055 outer membrane proteins. Antibodies to the 33.5 kD and 7.5 kD proteins were eluted when rabbit antisera were applied to an epoxy-activated Sepharose 6B column to which the outer membrane was coupled in the presence of dioxane. ELISA coats prepared with sonicated bacteria showed binding of the eluted antibodies with strains of all of seven different species of the enteric bacilli, but not with other Gram-negative bacilli or cocci, or with Gram-positive cocci; immunoblot analysis of transblots of SDS-PAGE-separated bacteria showed that antibodies to both of the 33.5 kD and 7.5 kD E. coli outer membrane proteins cross-reacted with the enteric bacilli of different species. Both of the anti-33.5 kD and -7.5 kD antibodies were bound by intact E. coli 055 cells, but more efficiently by sonically disrupted or heat-treated bacteria. The results show that affinity-purified anti-OM antibodies were useful for the study of the antigenic relatedness of E. coli OM proteins with proteins of other bacteria.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 4","pages":"257-63"},"PeriodicalIF":0.0,"publicationDate":"1986-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03050.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14652180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Chemical composition and biological properties of a lipopolysaccharide from Bacteroides intermedius. 中间拟杆菌脂多糖的化学组成和生物学特性。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-08-01 DOI: 10.1111/j.1699-0463.1986.tb03051.x

B Johne, K Bryn

{"title":"Chemical composition and biological properties of a lipopolysaccharide from Bacteroides intermedius.","authors":"B Johne, K Bryn","doi":"10.1111/j.1699-0463.1986.tb03051.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03051.x","url":null,"abstract":"Endotoxin (lipopolysaccharide, LPS) from an oral strain of Bacteroides intermedius was isolated by phenol extraction and purified by ultracentrifugation and gel filtration. The preparation was essentially free from contaminating nucleic acid and protein. The LPS contained rhamnose, fucose, mannose, glucose, galactose, glucosamine, and an unidentified sugar (approximate molar ratios 9:1:6:3:1:7:2). Neither heptose nor 2-keto-3-deoxyoctonate was detected. The major fatty acids were 3-hydroxy-15-methylhexadecanoic acid and 3-hydroxyhexadecanoic acid. The LPS was homogeneous with regard to molecular size, and its polysaccharide chain appeared short compared to the E. coli 055 LPS which was used as reference. A molecular weight of approximately 7,800 was estimated from gas chromatography data and by gel filtration in the presence of sodium deoxycholate. The B. intermedius LPS demonstrated low potency in the Limulus amoebocyte lysate assay, and in the chick embryo and mouse lethality tests and gave negative response in the local Shwartzman reaction.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 4","pages":"265-71"},"PeriodicalIF":0.0,"publicationDate":"1986-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03051.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14869533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Group JK diphtheroid bacteremia. The successive isolation of an antibiotic-susceptible and an antigenically different multi-resistant strain. JK组类白喉菌血症。连续分离出一株抗生素敏感菌株和一株抗原性不同的多重耐药菌株。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-08-01

O Heltberg, A Friis-Møller, H Ersgaard

{"title":"Group JK diphtheroid bacteremia. The successive isolation of an antibiotic-susceptible and an antigenically different multi-resistant strain.","authors":"O Heltberg, A Friis-Møller, H Ersgaard","doi":"","DOIUrl":"","url":null,"abstract":"A 71-year-old man with a permanent, subcutaneously implanted, intra-cardial pacemaker suffered from prolonged bacteremia with an antibiotic-susceptible group JK diphtheroid rod. He died in spite of the formation of specific serum antibody and parenteral treatment with ampicillin, cephradine and gentamicin. A second multi-resistant, but otherwise similar group JK strain was isolated post-mortem from the aseptically removed pacemaker electrode tip. The susceptible and the multi-resistant strains differed antigenically in crossed immunoelectrophoresis assays, and fatty acid isomer patterns were dissimilar. The theory that a multi-resistant group JK clone emerged by simple mutation in susceptible, indigenous group JK skin flora is rejected. The concept of major structural differences among group JK bacteria, possibly affecting cell-wall permeability, is supported. Crossed immunoelectrophoresis is suggested as a means for strain comparison in epidemiological surveys. Vancomycin is regarded as the antibiotic of choice for the treatment of potentially fatal, deep-seated infections.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 4","pages":"285-9"},"PeriodicalIF":0.0,"publicationDate":"1986-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14869535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical strains of Enterobacter agglomerans (synonyms: Erwinia herbicola, Erwinia milletiae) identified by DNA-DNA-hybridization. 应用dna - dna杂交技术鉴定聚簇肠杆菌临床菌株(除草厄尔温菌，千叶厄尔温菌)。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-08-01 DOI: 10.1111/j.1699-0463.1986.tb03043.x

E Lind, J Ursing

引用次数: 86

Same-day confirmation of Staphylococcus aureus bacteraemia by a thermonuclease test. 当日通过热核酸酶试验确诊金黄色葡萄球菌菌血症。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-08-01 DOI: 10.1111/j.1699-0463.1986.tb03055.x

K Bergh, J A Maeland

引用次数: 7

Population analyses of the susceptibility to ciprofloxacin of eight clinical strains of Pseudomonas aeruginosa. 8株铜绿假单胞菌临床菌株对环丙沙星的敏感性分析。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-08-01 DOI: 10.1111/j.1699-0463.1986.tb03052.x

P Gerner-Smidt, K Fuursted

引用次数: 2