Acta Pharmaceutica最新文献_第4页

MARS-5 vs. MARS-10: Optimizing pharmacist-led adherence assessment in clinical heart failure practice. MARS-5 vs. MARS-10：优化药师主导的临床心力衰竭实践依从性评估

IF 1.8 4区医学

Acta Pharmaceutica Pub Date : 2026-01-13 Print Date: 2025-12-01 DOI: 10.2478/acph-2025-0038

Mirna Momčilović, Iva Bužančić, Boško Skorić, Maja Ortner Hadžiabdić, Davor Miličić, Petra Turčić

{"title":"MARS-5 vs. MARS-10: Optimizing pharmacist-led adherence assessment in clinical heart failure practice.","authors":"Mirna Momčilović, Iva Bužančić, Boško Skorić, Maja Ortner Hadžiabdić, Davor Miličić, Petra Turčić","doi":"10.2478/acph-2025-0038","DOIUrl":"10.2478/acph-2025-0038","url":null,"abstract":"Optimising medication adherence is essential for effective heart failure (HF) management, yet nonadherence remains common, particularly among hospitalised and advanced-stage patients. This study evaluated the internal consistency reliability and score association of the Medication Adherence Report Scales, MARS-5 and MARS-10, self-report questionnaires in clinical pharmacist-led adherence assessments in hospitalised HF patients. Tools were administered during structured pharmacist-led interviews. To complement quantitative findings, four clinical cases were presented to illustrate the clinical relevance of adherence assessment in real--world HF management. Results showed a strong association between MARS-5 and MARS-10 (n = 70) responses (unweighted Cohen's kappa 0.820 (95 % CI 0.683-0.957; p < 0.001) for categorizing patients as nonadherent or adherent and Pearson's r coefficient of 0.899 (95 % CI 0.847-1.000; p < 0.001) for continuous score correlation), supporting score association and flexible use in clinical settings, with MARS-5 reliably identifying nonadherence (defined as score < 20 for MARS-5 and ≤ 8 for MARS-10), with potentially reduced respondent burden due to fewer items. Serious clinical complications were documented in nonadherent patients (41.43 % by MARS-5 and 35.71 % by MARS-10), illustrated through selected cases including stent thrombosis, embolic stroke, graft dysfunction, and deterioration in glycaemic control. These findings indicate the potential of MARS-5 as a practical, time-efficient tool for routine adherence assessment in acute settings. Case analyses underscore the critical role of the clinical pharmacist in proactively identifying nonadherence and enabling timely, targeted interventions to mitigate risk and improve patient outcomes in HF care.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"75 4","pages":"557-575"},"PeriodicalIF":1.8,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial and antibiofilm activities of naturally occurring naphthoquinones 2-hydroxy-1,4-naphthoquinone and 2-methoxy-1,4-naphthoquinone against Escherichia coli. 天然萘醌类2-羟基-1,4-萘醌和2-甲氧基-1,4-萘醌对大肠杆菌的抗菌和抗膜活性。

IF 1.8 4区医学

Acta Pharmaceutica Pub Date : 2026-01-13 Print Date: 2025-12-01 DOI: 10.2478/acph-2025-0041

Darija Stupin Polančec, Dafinë Kastrati Bajçinca, Ana-Marija Domijan

{"title":"Antibacterial and antibiofilm activities of naturally occurring naphthoquinones 2-hydroxy-1,4-naphthoquinone and 2-methoxy-1,4-naphthoquinone against Escherichia coli.","authors":"Darija Stupin Polančec, Dafinë Kastrati Bajçinca, Ana-Marija Domijan","doi":"10.2478/acph-2025-0041","DOIUrl":"10.2478/acph-2025-0041","url":null,"abstract":"The aim of this study was to evaluate the antibacterial and antibio-film potential of two naturally occurring naphthoquinones, 2-hydroxy-1,4-naphthoquinone (2-HNQ) and 2-methoxy-1,4-naphthoquinone (2-MNQ), against the Gram-negative bacterium Escherichia coli strain ATCC 25922. In the first step of the study, the minimum inhibitory concentrations (MICs) of 2-HNQ and 2-MNQ were determined using the microdilution method. Subsequently, possible mechanisms underlying the antibacterial activity of 2-HNQ and 2-MNQ against E. coli were investigated by assessing intracellular reactive oxygen species (ROS) production and membrane permeability. Finally, the impact of 2-HNQ and 2-MNQ on swarming motility and on pre- and post-biofilm formation of E. coli was evaluated. The MIC of 2-HNQ against E. coli was 500 µg mL-1, while that of 2-MNQ was 100 µg mL-1. Both compounds increased intracellular ROS production and altered the membrane permeability of E. coli. Moreover, 2-HNQ and 2-MNQ reduced swarming motility and inhibited both pre- and post-biofilm formation. The results of this study indicate that both naphthoquinones possess antibacterial potential, with 2-MNQ showing greater potency against E. coli. Their antibacterial activity appears to involve ROS generation and disruption of membrane permeability. Importantly, both tested naphthoquinones also impaired E. coli motility and bio-film formation, suggesting potential applications in the treatment of infections caused by E. coli.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"75 4","pages":"629-641"},"PeriodicalIF":1.8,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of clinical pharmacist counselling at discharge on adherence to oral antibiotics: A pilot study. 出院时临床药师咨询对口服抗生素依从性的影响：一项试点研究。

IF 1.8 4区医学

Acta Pharmaceutica Pub Date : 2026-01-13 Print Date: 2025-12-01 DOI: 10.2478/acph-2025-0039

Katarina Vilić, Mirna Momčilović, Darija Kuruc Poje, Iva Bužančić, Vesna Sertić, Gordana Šantek Zlatar, Igor Žabić, Maja Ortner Hadžiabdić, Petra Turčić

{"title":"Impact of clinical pharmacist counselling at discharge on adherence to oral antibiotics: A pilot study.","authors":"Katarina Vilić, Mirna Momčilović, Darija Kuruc Poje, Iva Bužančić, Vesna Sertić, Gordana Šantek Zlatar, Igor Žabić, Maja Ortner Hadžiabdić, Petra Turčić","doi":"10.2478/acph-2025-0039","DOIUrl":"10.2478/acph-2025-0039","url":null,"abstract":"Antibiotic resistance is a growing global health threat, with patient non-adherence to prescribed antibiotic regimens representing an important contributing factor. This prospective interventional study investigated the impact of clinical pharmacist counselling at hospital discharge on adherence to oral antibiotic therapy. The study was conducted at the Department of Nephrology and Endocrinology, General Hospital \"Dr. Tomislav Bardek\", Koprivnica, between March 2022 and July 2025. A total of 98 participants aged ≥18 years who were prescribed oral antibiotics at discharge were randomised into intervention (counselling) and non-intervention groups. Baseline socio-demographic and clinical data were collected, and adherence was assessed following treatment completion using a structured questionnaire developed by the authors. Of the 98 participants, 94 were included in the final analysis. Non-adherence was significantly lower in the intervention group, compared to the non-intervention group (2.1 % vs. 36.2 %, p < 0.001). Urinary tract infections represented the most common indication for antibiotic therapy, with ciprofloxacin and amoxicillin-clavulanic acid being the most frequently prescribed medicines. This study demonstrates that pharmacist-led discharge counselling markedly improves adherence to short-term antibiotic therapy. These findings provide preliminary evidence supporting integration of clinical pharmacists into hospital discharge processes to promote rational antibiotic use and combat antimicrobial resistance.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"75 4","pages":"643-657"},"PeriodicalIF":1.8,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The hidden impact of COVID-19 treatment strategies on the spread of Clostridioides difficile infection. COVID-19治疗策略对艰难梭菌感染传播的隐性影响。

IF 1.8 4区医学

Acta Pharmaceutica Pub Date : 2026-01-13 Print Date: 2025-12-01 DOI: 10.2478/acph-2025-0037

Ivana El Hassouni, Martin Kondža, Ivan Tomić, Ivona Ivančić, Ivan Zeljko, Ivica Brizić

{"title":"The hidden impact of COVID-19 treatment strategies on the spread of Clostridioides difficile infection.","authors":"Ivana El Hassouni, Martin Kondža, Ivan Tomić, Ivona Ivančić, Ivan Zeljko, Ivica Brizić","doi":"10.2478/acph-2025-0037","DOIUrl":"10.2478/acph-2025-0037","url":null,"abstract":"The COVID-19 pandemic introduced substantial changes to clinical practice, including widespread antibiotic use. These changes raised concerns about a potential rise in healthcare-associated infections, particularly Clostri dioi des difficile infection (CDI). This study aimed to investigate the hidden impact of COVID-19 treatment strategies on the incidence of CDI, with a specific focus on antibiotic use, advanced age, comorbidities, and the administration of proton pump inhibitors (PPIs) and corticosteroids. A retrospective observational study was conducted using anonymised patient data from the University Clinical Hospital Mostar. The number of CDI cases significantly increased during the COVID-19 peak in 2021, showing a perfect positive correlation with COVID-19 incidence (ρ = 1.0, p < 0.05). Antibiotic use was strongly associated with CDI (69 % vs. 12 %; p < 0.05), as was advanced age (≥ 65 years; 71 %; p < 0.05). The combined use of proton pump inhibitors and corticosteroids was significantly more frequent in the CDI group (54 % vs. 24 %; p < 0.05). The findings highlight how COVID-19 treatment strategies can unintentionally raise CDI risk, stressing the need for prudent antibiotic use, careful drug management and targeted prevention for elderly and high-risk patients.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"75 4","pages":"673-685"},"PeriodicalIF":1.8,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cholinergic regulation on polymeric immunoglobulin receptor expression in Caco-2 cells. 胆碱能对Caco-2细胞聚合免疫球蛋白受体表达的调控。

IF 1.4 4区医学

Acta Pharmaceutica Pub Date : 2025-10-10 Print Date: 2025-09-01 DOI: 10.2478/acph-2025-0033

German Higuera-Martínez, David Levaro-Loquio, Aldo Arturo Reséndiz-Albor, Ivonne Maciel Arciniega Martínez, Munich Guevara-Rubio, Maria Elisa Drago-Serrano, Judith Pacheco-Yepez

{"title":"Cholinergic regulation on polymeric immunoglobulin receptor expression in Caco-2 cells.","authors":"German Higuera-Martínez, David Levaro-Loquio, Aldo Arturo Reséndiz-Albor, Ivonne Maciel Arciniega Martínez, Munich Guevara-Rubio, Maria Elisa Drago-Serrano, Judith Pacheco-Yepez","doi":"10.2478/acph-2025-0033","DOIUrl":"https://doi.org/10.2478/acph-2025-0033","url":null,"abstract":"The polymeric immunoglobulin receptor (pIgR) mediates trans cytosis of IgA, a pivotal anti-inflammatory player of the mucosal immune system. Transcytosis mediated by pIgR entails protein effectors of vesicle-mediated transport involved in signal pathway activation that lead to the sorting of pIgR-IgA complexes from the basolateral to apical membrane. Each step of pIgR transport encompasses multiple targets for regulation, but the role of cholinergic system components, i.e. acetylcholine (ACh), the ligand of nicotinic (nAChR) and muscarinic (mAChR) receptors, is unclear. This study evaluated the effect of the cholinergic system on pIgR at transcriptional and protein levels. Accordingly, lipopolysaccharide (LPS)-primed Caco-2 cells were treated with nicotine (nAChR agonist) and/or mecamylamine (nAChR antagonist) or with muscarine (mAChR agonist) and/ or atropine (mAChR antagonist), and then pIgR was analysed in situ by immunofluorescence and by RT-qPCR. In general terms, cholinergic antagonists counteracted the upmodu latory outcome of both cholinergic agonists on both pIgR cellular location and mRNA levels. These findings suggest that the cholinergic system plays a key role in the regulation of epithelial immunity by modulating pIgR expression. The study provides insights into the interaction between the cholinergic system and intestinal immune mechanisms for future research in mucosal immunity and possible therapeutic strategies.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"75 3","pages":"547-555"},"PeriodicalIF":1.4,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145443697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scutellarin mitigates LPS-ATP-induced cardiomyocyte pyroptosis through the inhibition of the NLRP3/caspase-1/GSDMD signalling pathway. 黄芩苷通过抑制NLRP3/caspase-1/GSDMD信号通路减轻lps - atp诱导的心肌细胞焦亡。

IF 1.4 4区医学

Acta Pharmaceutica Pub Date : 2025-10-10 Print Date: 2025-09-01 DOI: 10.2478/acph-2025-0025

Xiao-Wei Li, Yun-Fei Chen, Lan Zhou, Peng Zhou, Peng Huang, Qian Niu, Jin-Cai Li

{"title":"Scutellarin mitigates LPS-ATP-induced cardiomyocyte pyroptosis through the inhibition of the NLRP3/caspase-1/GSDMD signalling pathway.","authors":"Xiao-Wei Li, Yun-Fei Chen, Lan Zhou, Peng Zhou, Peng Huang, Qian Niu, Jin-Cai Li","doi":"10.2478/acph-2025-0025","DOIUrl":"10.2478/acph-2025-0025","url":null,"abstract":"Scutellarin has a good myocardial protective effect. However, the underlying mechanism of scutellarin on cardiomyocyte pyroptosis remains unclear. In this study, we elucidated the mechanism of scutellarin to protect the injured myocardium. The molecular docking technique was used to predict the targets of scutellarin in protecting against myocardial injury. H9c2 cell pyroptosis was induced by lipopolysaccharide (LPS) and adenosine triphosphate (ATP). Then, the activities of CK and LDH were measured through a colourimetric assay. The level of cTnI was quantified by ELISA. mRNA expressions of NLRP3, cysteine-dependent aspartate-specific protease-1 (caspase-1), gasdermin D (GSDMD), interleukin-1β (IL-1β), and interleukin-18 (IL-18) were analyzed using RT-qPCR. Protein expressions of NLRP3, caspase-1, and GSDMD were detected by the immunofluorescence technique. Protein expression of NLRP3 was analysed by using Western blotting. Scutellarin had a good binding affinity with NLRP3, caspase-1, and GSDMD. Compared with LSP and ATP-treated cells, concentrations of 25, 50, and 100 µmol L-1 scutellarin reduced CK and LDH activities and the level of cTnI, decreased the mRNA expression of NLRP3, caspase-1, and GSDMD. In the mechanism study, scutellarin decreased mRNA expressions of NLRP3, caspase-1, GSDMD, IL-1β, and IL-18, and reduced the fluorescence expressions of NLRP3, caspase-1, and GSDMD. Scutellarin reduced the protein expression of NLRP3. Scutellarin inhibits myocardial cell pyroptosis induced by LPS and ATP, and the mechanism is related to the NLRP3/caspase-1/GSDMD signalling pathway.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":" ","pages":"517-530"},"PeriodicalIF":1.4,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of two different eco-friendly label-free platforms for analysis of selumetinib. 开发两种不同的环保无标签平台分析selumetinib。

IF 1.4 4区医学

Acta Pharmaceutica Pub Date : 2025-10-10 Print Date: 2025-09-01 DOI: 10.2478/acph-2025-0013

Sarah Alrubia, Wafa A Alshehri, Nourah Z Alzoman, Ibrahim A Darwish

{"title":"Development of two different eco-friendly label-free platforms for analysis of selumetinib.","authors":"Sarah Alrubia, Wafa A Alshehri, Nourah Z Alzoman, Ibrahim A Darwish","doi":"10.2478/acph-2025-0013","DOIUrl":"10.2478/acph-2025-0013","url":null,"abstract":"Selumetinib (SEL) is a recently approved medication for paediatric patients who have neurofibromatosis type 1. It is the first approved therapy for this rare, debilitating, and disfiguring disease. Development of proper analytical platforms for SEL analysis in its marketed pharmaceutical formulation (Koselugo® capsules) and blood plasma is highly warranted. Availability of such analytical tools would ensure SEL capsules' quality and effective therapy. This study introduces, for the first time, the development of two label-free and sensitive platforms for SEL quantification in capsules and human plasma. These platforms are microwave-assisted with an ultraviolet absorbance microplate reader (MW-UV) and reverse-phase high-performance liquid chromatography with a photodiode-array detector (HPLC-PDA). Both platforms employed the SEL native UV absorption as an analytical signal. The MW-UV measured the UV absorption in 96-well transparent plates at 255 nm. The HPLC-PDA involved chromatographic separation of SEL and tozasertib (TOZ), internal standard, on a C18 column both were detected at 255 nm. The optimum procedures of both platforms were established and validated following the ICH guidelines. The linearity ranges were 15-500 µg mL-1 and 0.8-100 µg mL-1, with limits of quantification of 15.3 and 3.5 µg mL-1, for MW-UV and HPLC-PDA, resp. Both platforms displayed high precision with relative standard deviation values ≤ 1.8 %, and high accuracy with recovery ranging from 98.3 to 102.3 %. The platforms were successfully applied to quantify SEL in bulk form, Koselugo® capsules, and were preliminarily applied to human plasma analysis. Eco-friendliness assessment confirmed the adherence of both platforms to green analytical approaches. MW-UV and HPLC-PDA are simple and fast, enabling high-throughput analysis, thus introducing valuable tools for routine use in quality control and clinical laboratories for SEL quantification.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":" ","pages":"407-426"},"PeriodicalIF":1.4,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer activity of Lavandula stoechas L. flower ethanolic extract through apoptotic pathway modulation in colorectal cancer cells. 薰衣草花乙醇提取物通过凋亡通路调控结直肠癌细胞的抗癌活性。

IF 1.4 4区医学

Acta Pharmaceutica Pub Date : 2025-10-10 Print Date: 2025-09-01 DOI: 10.2478/acph-2025-0034

Zeynep Doğru, Mustafa Selim Doğru, Gamze Yeşilay, Özgecan Kayalar, Mahfuz Elmastaş

{"title":"Anticancer activity of Lavandula stoechas L. flower ethanolic extract through apoptotic pathway modulation in colorectal cancer cells.","authors":"Zeynep Doğru, Mustafa Selim Doğru, Gamze Yeşilay, Özgecan Kayalar, Mahfuz Elmastaş","doi":"10.2478/acph-2025-0034","DOIUrl":"https://doi.org/10.2478/acph-2025-0034","url":null,"abstract":"This study aimed to investigate the anticancer effects and underlying mechanisms of an ethanolic extract of Lavandula stoechas L. flowers (LsHE) on colorectal cancer. The extract demon strated high phenolic content (230.31 ± 11.28 mg GAE g-¹ dm) and strong antioxidant activity. HPLC analysis identified rosmarinic acid and quercetin as major constituents. HT-29 colorectal carcinoma cells and HEK-293 healthy kidney epithelial cells were treated with LsHE for 48 h. The concentration of LsHE required to inhibit 50 % of HT-29 cell viability was found to be 86.37 ± 3.07 µg mL-1, whereas a higher concentration of 131.30 ± 9.33 µg mL-1 was observed for HEK-293 cells. In HT-29 cells, flow cytometry analysis revealed increased early (9.7 %) and late (6.8 %) apoptotic populations following LsHE treatment (p < 0.0001). qRT-PCR analysis showed significant upregulation of TP53 and CASP3 compared to the untreated group (p < 0.05 and p < 0.01, resp.), while BAX expression was unexpectedly downregulated. These findings suggest that LsHE may trigger caspase-3-dependent apoptosis through a p53-mediated mechanism, potentially independent of the BAX/BCL-2 pathway. In conclusion, the present in vitro study highlights the potential of LsHE as a natural agent that still exerts some cytotoxicity toward normal epithelial cells and pro-apoptotic activity in colorectal cancer cells. Our findings provide a molecular basis for further in vivo studies to evaluate the possible therapeutic potential and mechanistic relevance of LsHE in CRC chemoprevention.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"75 3","pages":"449-468"},"PeriodicalIF":1.4,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145443591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemometrically-supported quality assessment of chamomile tea. 化学计量学支持的甘菊茶质量评价。

IF 1.4 4区医学

Acta Pharmaceutica Pub Date : 2025-10-10 Print Date: 2025-09-01 DOI: 10.2478/acph-2025-0028

Biljana Blažeković, Ines Job Čičin-Mašansker, Marija Kindl, Lucia Mahovlić, Sanda Vladimir-Knežević

{"title":"Chemometrically-supported quality assessment of chamomile tea.","authors":"Biljana Blažeković, Ines Job Čičin-Mašansker, Marija Kindl, Lucia Mahovlić, Sanda Vladimir-Knežević","doi":"10.2478/acph-2025-0028","DOIUrl":"https://doi.org/10.2478/acph-2025-0028","url":null,"abstract":"The quality of chamomile (Matricaria recutita) is largely determined by its content of essential oils and flavonoids, the main pharmacologically active constituents. In this study, the phyto-chemical profiling of 22 commercially available chamomile flower tea samples was aided by chemometrics, comparing loose teas of whole heads with tea bags containing comminuted flowers. Principal component analysis (PCA) and agglomerative hierarchical clustering (AHC), which included both essential oil and flavonoid constituents, showed that chamomile teas can be well-differentiated and categorised into two groups that are closely related to the pharmaceutical form and largely explain the influence of processing. Multivariate analyses of the phytochemical data matrix showed clear differences between loose chamomile tea and tea bags, with the former having a more consistent composition and an overall higher quality. The essential oil content varied widely (0.75-5.34 mL kg-1), with only five loose teas exceeding the minimum content specified in the European Pharmacopoeia (≥ 4 mL kg-1), while most tea bag samples did not fulfil this requirement. GC-MS analyses of essential oils revealed sesquiterpenes as predominant constituents, assigning all samples to the bisabolol oxide-rich chemotype. The total flavonoid content determined by UV/Vis spectrophotometry ranged from 0.17 to 0.55 %, whereas RP-HPLC/DAD analysis revealed that the levels of apigenin-7-glucoside in tea bag samples often did not meet pharmacopoeial standards. Partial least squares-discriminant analysis (PLS-DA) yielded a robust and statistically significant model, showing for the first time that the quality differences between loose teas and tea bags can be explained by at least four key components. These results highlight the utility of chemometric tools in chamomile quality assessment and emphasise the need for improved standardisation that supports the preference for whole flower teas to ensure therapeutic efficacy.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"75 3","pages":"331-355"},"PeriodicalIF":1.4,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145443638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Niosome-based delivery systems for olanzapine: Formulation, characterisation, and kinetic evaluation. 奥氮平的niosome基给药系统：配方、表征和动力学评价。

IF 1.4 4区医学

Acta Pharmaceutica Pub Date : 2025-10-10 Print Date: 2025-09-01 DOI: 10.2478/acph-2025-0026

Samiah Alhabardi, Basmah Aldosari, Gadah Al-Hamoud, Shog Moahmmed Alali, Reema Al Khbiah, Lama Albulayhi, Wedad Sarawi, Naifa Alenazi

{"title":"Niosome-based delivery systems for olanzapine: Formulation, characterisation, and kinetic evaluation.","authors":"Samiah Alhabardi, Basmah Aldosari, Gadah Al-Hamoud, Shog Moahmmed Alali, Reema Al Khbiah, Lama Albulayhi, Wedad Sarawi, Naifa Alenazi","doi":"10.2478/acph-2025-0026","DOIUrl":"10.2478/acph-2025-0026","url":null,"abstract":"This study investigates the development and characterisation of niosome-based delivery systems for olanzapine, an antipsychotic drug. Niosomes were prepared using various grades of Span surfactants (Span 60, Span 40, and Span 20) in combination with cholesterol at different ratios. The formulations were characterised in terms of particle size, polydispersity index, zeta potential, and encapsulation efficiency. Results showed an inverse relationship between surfactant hydrophilic-lipophilic balance (HLB) values and niosome size, with Span 60 producing the smallest vesicles. Optimal formulations were achieved with a 1:1 ratio of surfactant to cholesterol. Span 60 niosomes exhibited the highest encapsulation efficiency (up to 81 ± 2.5 %) and the most negative zeta potential, indicating superior stability. In vitro release studies demonstrated sustained release profiles for all niosomal formulations compared to the free drug, with Span 60 formulations showing the slowest release rates. Release kinetics analysis revealed a Fickian diffusion-controlled mechanism best described by the Korsmeyer-Peppas model. These findings suggest that niosomal formulations, particularly those based on Span 60, offer a promising approach for improving olanzapine delivery, potentially enhancing its bioavailability and therapeutic efficacy in the treatment of psychiatric disorders.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":" ","pages":"531-545"},"PeriodicalIF":1.4,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0