{"title":"Assisted conception and immunological infertility.","authors":"C Cimino, G Catalano, E Cittadini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Our work with a group involved in Assisted Conception (AC) has provided us with a perfect opportunity for making an assessment of the efficiency of the techniques used for the treatment of Male Antisperm Autoimmunization (MAA) and Female Antisperm Isoimmunization (FAI). The aim of this study is to present all the most important methods of AC used by our group for the treatment of immunological infertility, such as intrauterine insemination, intraperitoneal insemination, intrafallopian gamete transfer, in-vitro insemination and intrauterine embryo transfer, intrafallopian zygote transfer, intrafallopian embryo transfer, and gamete micromanipulation. In-vitro fecundation would appear to be particularly efficient in cases of FAI, while MAA would seem to benefit more from the use of gamete micromanipulation.</p>","PeriodicalId":7016,"journal":{"name":"Acta Europaea fertilitatis","volume":"26 1","pages":"13-20"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19887919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Clinical use of the Clearplan ovulation test in the monitoring of spontaneous and clomiphene-induced cycles.","authors":"D Pungetti, U Escari, M Lenzi, E Zanardi","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7016,"journal":{"name":"Acta Europaea fertilitatis","volume":"26 1","pages":"25-9"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19887921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Techniques of sperm selection and in vitro fertilization results.","authors":"T Leonetti, F Causio, G Cagnazzo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study was performed to evaluate the effectiveness of four techniques for sperm selection in vitro fertilization (IVF) programme. Swim Up, Percoll, Sedimentation and Mini-Percoll were evaluated in 440 IVF cycles. The various methods were performed according to the motility and the number of spermatozoa, and the degree of cellularity of the sample; Swim Up and Percoll were used when sperm count was superior 20 million/ml, Sedimentation and Mini-Percoll when the sperm count was less than 20 million/ml. There was no difference in fertilization rate and in the number of replaced embryos per transfer with Swim Up compared to Percoll procedure; the Sedimentation technique improved fertilization (p < 0.05) and embryos rate (p < 0.001) compared to Mini-Percoll sperm preparation. There was no difference in pregnancy rate with Swim Up compared to Percoll and Sedimentation compared to Mini-Percoll procedure. We conclude that with advanced reproductive procedures where serious sperm disorders exist, the choose of the tecnique of sperm selection, most appropriate, according with the motility and the number of spermatozoa, the debrits and nonspermatic cells of the semen, might produce a suspension of spermatozoa with high fertilization potential.</p>","PeriodicalId":7016,"journal":{"name":"Acta Europaea fertilitatis","volume":"26 1","pages":"31-4"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19887819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Incidence and outcome of multiple pregnancy after in vitro fertilization.","authors":"F Causio, T Leonetti, M Falagario","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of this study was to analyse the experience of IVF multiple pregnancies, in relation to the maternal morbidity and the neonatal morbidity and mortality. We considered 48 multiple pregnancies: 36 twins (group A), 8 triplets (group B), 2 quadruplets (group C), and 2 quintuplets (group D). The mean maternal age was 29.72 years and the mean gestational age was 36.83 weeks. Of the 36 patients with two babies, 20 (55.60%) had cesarean section, whereas 12 were delivered vaginally. All the triplets, quadruplets and quintuplets were delivered abdominally. Twenty-three cervical cerclage were placed. In group A 7 patients had premature rupture of the membranes (PROM), 2 had pregnancy-induced hypertension (PIH) and 14 had premature labour. In group B 4 patients had premature labour, 3 hadPROM and 2 had PIH. In group C one patient had premature labour. In group D 2 patients had premature labour and one also PIH. Multiple pregnancies lead to a high incidence of antenatal complications and extensive neonatal morbidity which translate into prolonged and expensive hospitalization for the neonates.</p>","PeriodicalId":7016,"journal":{"name":"Acta Europaea fertilitatis","volume":"26 1","pages":"41-4"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19887821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C Fiçicioğlu, S Tasdemir, P F Arioglu, R Unlu, C Yorganci
{"title":"The use of transvaginal ultrasonography in the evaluation of luteal phase endometrium.","authors":"C Fiçicioğlu, S Tasdemir, P F Arioglu, R Unlu, C Yorganci","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the use of transvaginal ultrasonography, a non invasive method, for the evaluation of endometrium, to diagnose luteal phase insufficiency.</p><p><strong>Design: </strong>Prospective, randomized, clinical study.</p><p><strong>Setting: </strong>Human volunteers in an infertility clinic.</p><p><strong>Patients: </strong>Patients who applied to the infertility clinic undergoing diagnostic procedures for their complaints.</p><p><strong>Interventions: </strong>Follicle maturation, ovulation and endometrial grades were studied with transvaginal ultrasonography. Endometrial sampling was performed during midluteal and late luteal phase. Progesterone and estradiol levels were measured in the blood drawn on the sampling day.</p><p><strong>Main outcome measures: </strong>Endometrial grade, histopathology, estradiol and progesterone levels.</p><p><strong>Results: </strong>In both groups, as the endometrial grades increased, the rate of normal histopathological results increased significantly. There was no statistically significant correlation between endometrial thickness and progesterone and estradiol levels.</p><p><strong>Conclusions: </strong>Evaluation of stromal maturation with ultrasonography is successful, but cannot replace endometrial sampling in evaluating luteal phase endometrium.</p>","PeriodicalId":7016,"journal":{"name":"Acta Europaea fertilitatis","volume":"26 1","pages":"35-40"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19887822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R K Natwar, A Mann, R K Sharma, W Aulitzky, J Frick
{"title":"Effect on human gamma interferon on mice testis: a quantitative analysis of the spermatogenic cells.","authors":"R K Natwar, A Mann, R K Sharma, W Aulitzky, J Frick","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Effect of Human Gamma Interferon (Hu-IFN-gamma) on the testicular histology was studied in mice. Male mice were administered Hu-IFN-gamma intratesticularly at the doses of 2, 10 and 20 micrograms/testis in a volume of 1.0 microliter isotonic normal saline. Contralateral testis served as control and was administered same amount of vehicle. All the animals were sacrificed 7 days after drug administration. Body weight and the weights of testis and epididymis were not affected by IFN treatment nor was there any effect of the drug on the motility of the vas deferens spermatozoa. Low dose of IFN (3 mukg) did not have significant effect on the histoarchitecture of the testes and various spermatogenic elements, a progressive damage was however observed with the increasing doses of IFN. Pronounced deleterious effect of IFN on the testis leading to desquamation of the germinal epithelium, reduction in the germinal cell height and tubular diameter was observed with 20 micrograms dose. Quantitative studies on seminiferous epithelium showed a significant decrease in the number of Sertoli cells, stage-7 spermatids and stage-16 spermatozoa. The ratios of resting type spermatocyte: type A spermatogonia and stage-7 spermatids: pachytene spermatocyte was also reduced. The ratios of pachytene spermatocyte: resting spermatocyte and stage-16 spermatozoa: stage-7 spermatids were however not affected by IFN treatment. In another experiment IFN was administered (2 micrograms/day) subcutaneously to male mice for 30 days. No effect of drug treatment on body weight, organ weight, sperm motility and histology (including morphometry) of the testis was observed. Our data suggest that IFN action at testis may be associated with the antiproliferative effect of interferon.</p>","PeriodicalId":7016,"journal":{"name":"Acta Europaea fertilitatis","volume":"26 1","pages":"45-9"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19887824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F Ubaldi, Z Nagy, J Liu, H Tournaye, M Camus, J Smitz, I Liebaers, P Devroey, A Van Steirteghem
{"title":"A survey of four years of experience with intracytoplasmic sperm injection.","authors":"F Ubaldi, Z Nagy, J Liu, H Tournaye, M Camus, J Smitz, I Liebaers, P Devroey, A Van Steirteghem","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present report covers the results of a 38-month period in which 2853 consecutive intracytoplasmic sperm injection (ICSI) cycles were performed in 1953 couples. These couples were afflicted with male factor infertility and had at least one previous failed conventional in vitro fertilization (IVF) treatment cycle. In other couples, the husband had semen parameters incompatible with conventional IVF or suffered from excretory or secretory azoospermia where it was possible to recover spermatozoa by microsurgical epididymal sperm aspiration (mesa) or by testicular sperm extraction (tese) procedure. Overall, the 2-PN fertilization rate was 62% per retrieved metaphase II oocyte and 70% per successfully injected metaphase II oocyte. Embryo transfer was performed in 91% of started cycles. The cumulative pregnancy rate (positive HCG) was 34% per started ICSI treatment and 37% per embryo transfer.</p>","PeriodicalId":7016,"journal":{"name":"Acta Europaea fertilitatis","volume":"26 1","pages":"7-11"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19887920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pentoxifylline in male-factor infertility: its therapeutic efficacy after oral administration.","authors":"B Faka, M Api, C Fiçicioğlu, A Gürbüz, O Oral","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To determine whether the oral use of pentoxifylline improves the fertilization ability, concentration and motility of spermatozoa of poor fertilizer male subject.</p><p><strong>Design: </strong>Analysis of the spermiogram parameters before and after oral pentoxifylline administration.</p><p><strong>Setting: </strong>Zeynep Kamil Women and Children's Hospital, Department of Infertility.</p><p><strong>Patients: </strong>Fourteen healthy, untreated male partners of couples who applied to our infertility department and were found to have poor semen parameters.</p><p><strong>Main outcome measures: </strong>Data of the semen analyses and fertilization rates are compared before and after treatment.</p><p><strong>Results: </strong>Before the treatment, the average percentage sperm motility and density were 30.1 +/- 8 (%) and 14.8 +/- 3.2 (10(6)/ml), respectively. The same parameters were found to be 37.8 +/- 13 (%) and 19.5 +/- 10 (10(6)/ml), respectively, following the treatment. Not significant, p = 0.08 and p = 0.116, respectively).</p><p><strong>Conclusion: </strong>None of the semen parameters evaluated were improved by oral administration of pentoxifylline. Therefore pentoxifylline does not increase percentage motility and density of spermatozoa or the fertilization rates.</p>","PeriodicalId":7016,"journal":{"name":"Acta Europaea fertilitatis","volume":"25 6","pages":"351-3"},"PeriodicalIF":0.0,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19806680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y Prapas, N Prapas, A Chatziparasidou, P Konstantinou, G Vlassis
{"title":"Ovarian hyperstimulation syndrome and heterotopic pregnancy after IVF.","authors":"Y Prapas, N Prapas, A Chatziparasidou, P Konstantinou, G Vlassis","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We report a case of Severe OHSS combined with Heterotopic pregnancy. During the treatment of OHSS the patient underwent transvaginal paracentesis for the ascites but no sign of blood was noticed into the fluid. The ultrasound control in the 8th week had shown a normal intrauterine pregnancy without any suspicion for heterotopic pregnancy. The diagnosis for heterotopic pregnancy became possible at 10 Weeks of gestation by Ultrasound. Laparotomy with partial tubal resection was performed. The intrauterine pregnancy is still ongoing without complications.</p>","PeriodicalId":7016,"journal":{"name":"Acta Europaea fertilitatis","volume":"25 6","pages":"331-3"},"PeriodicalIF":0.0,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19806679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}