Acta Biologica Hungarica最新文献_第8页

Hydrogen peroxide induced oxidative damage on mechanical properties of the articular cartilage. 过氧化氢诱导关节软骨机械性能的氧化损伤。

Acta Biologica Hungarica Pub Date : 2017-12-01 DOI: 10.1556/018.68.2017.4.3

Ekrem Cicek

引用次数: 4

Electro-antennographic response of Helopeltis theivora to synthetic pesticides used in tea plantations. 茶园合成农药对黑绒猴触角电反应的影响。

Acta Biologica Hungarica Pub Date : 2017-12-01 DOI: 10.1556/018.68.2017.4.2

Kokkadan K Srikumar, Sukumaran Smitha, Bastian S Kumar, Balakrishnan Radhakrishnan

引用次数: 3

How arbuscular mycorrhizal fungi influence the defense system of sunflower during different abiotic stresses. 丛枝菌根真菌如何影响向日葵在不同非生物胁迫下的防御系统。

Acta Biologica Hungarica Pub Date : 2017-12-01 DOI: 10.1556/018.68.2017.4.4

Zoltán Mayer, Nguyen Hong Duc, Zita Sasvári, Katalin Posta

引用次数: 12

Comparison of the production and chemical constituents of five Perilla frutescens (L.) Britt. accessions. 5种紫苏的生产及化学成分比较布瑞特。登记入册。

Acta Biologica Hungarica Pub Date : 2017-12-01 DOI: 10.1556/018.68.2017.4.10

Péter Radácsi, Szilvia Sárosi, Lotti Ágnes Szomor, Éva Németh-Zámbori

{"title":"Comparison of the production and chemical constituents of five Perilla frutescens (L.) Britt. accessions.","authors":"Péter Radácsi, Szilvia Sárosi, Lotti Ágnes Szomor, Éva Németh-Zámbori","doi":"10.1556/018.68.2017.4.10","DOIUrl":"https://doi.org/10.1556/018.68.2017.4.10","url":null,"abstract":"An open field experiment was carried out with five purple Perilla frutescens accessions (588P, GB, J3, JTD3, PS3) in 2014 and 2015. Morphological traits, production, total phenolic content (TPC), essential oil content (EOC) and composition as well as the antioxidant capacity (AOC) were investigated. Highest biomass was produced by JTD3 in both years. The antioxidant capacity and total phenolic content in the stems was lower than in the leaves in all accessions. Leaves of accession GB produced the highest AOC values (215.594 ± 1.437 in 2014 and 86.609 ± 3.602 mg AAE g-1 in 2015, respectively) while the strain 588P showed the lowest values (139.544 ± 1.934 in 2014 and 38.966 ± 4.569 mg AAE g-1 in 2015, respectively). The highest TPC values were measured by PS3 in 2014 (204.320 ± 1.822 mg GAE g-1) and GB in 2015 (136.450 ± mg GAE g-1). The 588P produced the highest essential oil content (1.432 ml 100 g-1 DM) while J3 had the lowest (0.144 ml 100 g-1 DM) in both years. Strong positive correlation was found between the density of glandular hairs and the essential oil content. Three accessions (588P, J3, JTD3) belong to the perillaldehyde chemotype while GB and PS3 to the dehydro elsholtzia ketone chemotype. All studied accessions can be cultivated in Hungary. For the biomass production the JTD3, while for the essential oil production the 588P can be recommended.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 4","pages":"453-465"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.4.10","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35675347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Establishment of an efficient plant regeneration culture protocol and achievement of successful genetic transformation in Jatropha curcas L. 麻疯树高效植株再生培养方案的建立及遗传转化的成功实现。

Acta Biologica Hungarica Pub Date : 2017-12-01 DOI: 10.1556/018.68.2017.4.8

Ying Liu, Guoxuan Liu, Yali Yang, Sufang Niu, Fuguang Yang, Shaoxia Yang, Jianian Tang, Jianping Chen

{"title":"Establishment of an efficient plant regeneration culture protocol and achievement of successful genetic transformation in Jatropha curcas L.","authors":"Ying Liu, Guoxuan Liu, Yali Yang, Sufang Niu, Fuguang Yang, Shaoxia Yang, Jianian Tang, Jianping Chen","doi":"10.1556/018.68.2017.4.8","DOIUrl":"https://doi.org/10.1556/018.68.2017.4.8","url":null,"abstract":"An efficient and reproducible protocol is described for shoot-bud regeneration and Agrobacterium tumefaciens-mediated genetic transformation of J. curcas. Treating the explants with high concentrations (5-120 mg/L) of TDZ for short durations (5-80 min) before inoculation culture increased significantly the regeneration frequency and improved the quality of the regenerated buds. The highest shoot-buds induction rate (87.35%) was achieved when petiole explants were treated with 20 mg/L TDZ solution for 20 min and inoculated on hormone-free MS medium for 30 days. Regenerated shoots of 0.5 cm or a little longer were isolated and grafted to seedling stocks of the same species, and then the grafted plantlets were planted on half-strength MS medium containing 0.1 mg/L IBA and 2 mg/L sodium nitroprusside (SNP). This grafting strategy was found to be very effective, to obtain that healthy grafted plantlets ready for acclimatization within 20 days. By the above mentioned protocol and with general Agrobacterium - mediated genetic transformation methods only 65 days were needed to obtain intact transgenic plants.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 4","pages":"428-442"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.4.8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35675248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Inhibitory effects of four neonicotinoid active ingredients on acetylcholine esterase activity. 四种新烟碱类活性成分对乙酰胆碱酯酶活性的抑制作用。

Acta Biologica Hungarica Pub Date : 2017-12-01 DOI: 10.1556/018.68.2017.4.1

János Győri, Anna Farkas, Oksana Stolyar, András Székács, Mária Mörtl, Ágnes Vehovszky

{"title":"Inhibitory effects of four neonicotinoid active ingredients on acetylcholine esterase activity.","authors":"János Győri, Anna Farkas, Oksana Stolyar, András Székács, Mária Mörtl, Ágnes Vehovszky","doi":"10.1556/018.68.2017.4.1","DOIUrl":"https://doi.org/10.1556/018.68.2017.4.1","url":null,"abstract":"There is a great concern about the decline of pollinators, and neonicotinoids emerging bee disorders are assumed to play a significant role. Since changes in learning ability has been observed in honey bees exposed to some acetylcholine esterase (AChE) inhibitors, we therefore, tested in vitro the effect of four neonicotinoids on purified eel AChE. AChE activity was inhibited in a concentration-dependent manner, and calculated IC50 values for thiamethoxam (IC50 = 414 μM) and clothianidin (IC50 = 160 μM) were found to be much higher compared to acetamiprid (IC50 = 75.2 μM) and thiacloprid (IC50 = 87.8 μM). The Lineweaver-Burk reciprocal plots for acetamiprid shows unchanged Vmax and increased Km values with inhibitor concentrations, while analysis of Michaelis-Menten plots shows predominantly competitive mechanism. The inhibition constant value (Ki = 24.3 μM) indicates strong binding of the acetamiprid complex to AChE. Finally, the four tested neonicotinoids are not a uniform group regarding their blocking ability. Our results suggest a previously not established, direct AChE blocking mechanism of neonicotinoids tested, thus the neuronal AChE enzyme is likely among the direct targets of the neonicotinoid insecticides. We conclude, that these AChE inhibitory effects may also contribute to toxic effects on the whole exposed animal.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 4","pages":"345-357"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.4.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35675245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

In vivo screening of diterpene alkaloids using bdelloid rotifer assays. 利用蛭形轮虫法在体内筛选二萜生物碱。

Acta Biologica Hungarica Pub Date : 2017-12-01 DOI: 10.1556/018.68.2017.4.9

Tivadar Kiss, Lilla Mácsai, Dezső Csupor, Zsolt László Datki

引用次数: 6

Isolation and quantitative analysis of physalin D in the fruit and calyx of Physalis alkekengi L. 酸浆果和花萼中physalin D的分离与定量分析。

Acta Biologica Hungarica Pub Date : 2017-09-01 DOI: 10.1556/018.68.2017.3.7

Eszter Laczkó-Zöld, Péter Forgó, István Zupkó, Eşianu Sigrid, Judit Hohmann

{"title":"Isolation and quantitative analysis of physalin D in the fruit and calyx of Physalis alkekengi L.","authors":"Eszter Laczkó-Zöld, Péter Forgó, István Zupkó, Eşianu Sigrid, Judit Hohmann","doi":"10.1556/018.68.2017.3.7","DOIUrl":"https://doi.org/10.1556/018.68.2017.3.7","url":null,"abstract":"Physalin D was isolated from the methanol extract of Physalis alkekengi L. fruits by combination of different chromatographic methods (CPC, TLC, HPLC). The structure was elucidated based on 1H and 13C NMR spectral analysis with the aid of 2D-correlation spectroscopy (1H, 1H-COSY, HSQC and HMBC) and comparison with literature data. The quantity of physalin D in mature and immature fruits and calyces was determined by RP-HPLC-UV method. Among the studied samples, immature calyx showed the highest content of physalin D (0.7880 ± 0.0612%), while mature calyx contained 4 times less amount (0.2028 ± 0.016%). The physalin D content of the fruit was much lower; immature fruits contained 0.0992 ± 0.0083% physalin D and mature fruits 0.0259 ± 0.0021%. The antiproliferative activity of the CHCl3 extract and its fractions was tested on three cancer cell lines (HeLa, MCF-7 and A431). The antiproliferative activity of physalin D is discussed with regard the published data.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 3","pages":"300-309"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.3.7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35399477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Morphometric characteristics and COI haplotype diversity of Arctodiaptomus spinosus (Copedoda) populations in soda pans in Hungary. 匈牙利盐碱地棘齿兽种群形态计量学特征及COI单倍型多样性

Acta Biologica Hungarica Pub Date : 2017-09-01 DOI: 10.1556/018.68.2017.3.5

László Forró, Judit Nédli, Enikő Csata, Virág Krízsik, Csilla Balogh, László G-Tóth

{"title":"Morphometric characteristics and COI haplotype diversity of Arctodiaptomus spinosus (Copedoda) populations in soda pans in Hungary.","authors":"László Forró, Judit Nédli, Enikő Csata, Virág Krízsik, Csilla Balogh, László G-Tóth","doi":"10.1556/018.68.2017.3.5","DOIUrl":"https://doi.org/10.1556/018.68.2017.3.5","url":null,"abstract":"Arctodiaptomus spinosus (Daday, 1891) is a characteristic species of the soda pan zooplankton in the Great Hungarian Plain. The biogeographical distribution of the species is interesting, since its range expands from the Pannonian Biogeographic region to the other side of the Carpathians, occurring in saline lakes in Eastern Anatolia, Armenia, Iran and in temporary waters in Ukraine. Our investigations focused on the morphometric characteristics and the COI haplotype diversity of four Hungarian populations in the Kiskunság area. We detected substantial morphological differences between the Böddi-szék population and the rest of the sampling sites, however considerable differences were not observable in the COI haplotypes in the populations. The 20 animals investigated for COI haplotypes belonged to the same haplotype network. Tajima's D indicated departures from the neutral Wright - Fisher population model and suggested population expansion. The genetic composition of Arctodiaptomus spinosus populations in the Kiskunság area is rather uniform.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 3","pages":"279-289"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.3.5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35399400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In vitro activity of calcium channel blockers in combination with conventional antifungal agents against clinically important filamentous fungi. 钙通道阻滞剂联合常规抗真菌药物对临床重要丝状真菌的体外活性研究。

Acta Biologica Hungarica Pub Date : 2017-09-01 DOI: 10.1556/018.68.2017.3.10

Mónika Homa, Kinga Hegedűs, Ádám Fülöp, Vanessza Wolfárt, Shine Kadaikunnan, Jamal M Khaled, Naiyf S Alharbi, Csaba Vágvölgyi, László Galgóczy

{"title":"In vitro activity of calcium channel blockers in combination with conventional antifungal agents against clinically important filamentous fungi.","authors":"Mónika Homa, Kinga Hegedűs, Ádám Fülöp, Vanessza Wolfárt, Shine Kadaikunnan, Jamal M Khaled, Naiyf S Alharbi, Csaba Vágvölgyi, László Galgóczy","doi":"10.1556/018.68.2017.3.10","DOIUrl":"https://doi.org/10.1556/018.68.2017.3.10","url":null,"abstract":"Despite the current therapeutic options, filamentous fungal infections are associated with high mortality rate especially in immunocompromised patients. In order to find a new potential therapeutic approach, the in vitro inhibitory effect of two antiarrhythmic agents, diltiazem and verapamil hydrochloride were tested against different clinical isolates of ascomycetous and mucoralean filamentous fungi. The in vitro combinations of these non-antifungal drugs with azole and polyene antifungal agents were also examined. Susceptibility tests were carried out using the broth microdilution method according to the instructions of the Clinical and Laboratory Standards Institute document M38-A2. Checkerboard microdilution assay was used to assess the interactions between antifungal and non-antifungal drugs. Compared to antifungal agents, diltiazem and verapamil hydrochloride exerted a relatively low antifungal activity with high minimal inhibitory concentration values (853-2731 μg/ml). Although in combination they could increase the antifungal activity of amphotericin B, itraconazole and voriconazole. Indifferent and synergistic interactions were registered in 33 and 17 cases, respectively. Antagonistic interactions were not revealed between the investigated compounds. However, the observed high MICs suggest that these agents could not be considered as alternative systemic antifungal agents.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 3","pages":"334-344"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.3.10","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35399475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6