钙通道阻滞剂联合常规抗真菌药物对临床重要丝状真菌的体外活性研究。

Q3 Biochemistry, Genetics and Molecular Biology
Mónika Homa, Kinga Hegedűs, Ádám Fülöp, Vanessza Wolfárt, Shine Kadaikunnan, Jamal M Khaled, Naiyf S Alharbi, Csaba Vágvölgyi, László Galgóczy
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引用次数: 6

摘要

尽管目前的治疗选择,丝状真菌感染与高死亡率相关,特别是在免疫功能低下的患者中。为了寻找新的治疗途径,研究了地尔硫卓和盐酸维拉帕米两种抗心律失常药物对不同临床分离的子囊菌和粘膜丝状真菌的体外抑制作用。研究了这些非抗真菌药物与唑类和多烯类抗真菌药物的体外联合作用。药敏试验采用肉汤微量稀释法,按照临床和实验室标准协会文件M38-A2的说明进行。采用棋盘微稀释法评价抗真菌药物与非抗真菌药物的相互作用。与抗真菌药物相比,地尔硫卓和盐酸维拉帕米的抑菌活性相对较低,最低抑菌浓度较高(853 ~ 2731 μg/ml)。伊曲康唑和伏立康唑联合使用可提高两性霉素B、两性霉素B和伏立康唑的抗真菌活性。分别有33例和17例出现无差异和协同作用。在所研究的化合物之间未发现拮抗相互作用。然而,观察到的高mic表明这些药物不能被认为是替代的全身抗真菌药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro activity of calcium channel blockers in combination with conventional antifungal agents against clinically important filamentous fungi.

Despite the current therapeutic options, filamentous fungal infections are associated with high mortality rate especially in immunocompromised patients. In order to find a new potential therapeutic approach, the in vitro inhibitory effect of two antiarrhythmic agents, diltiazem and verapamil hydrochloride were tested against different clinical isolates of ascomycetous and mucoralean filamentous fungi. The in vitro combinations of these non-antifungal drugs with azole and polyene antifungal agents were also examined. Susceptibility tests were carried out using the broth microdilution method according to the instructions of the Clinical and Laboratory Standards Institute document M38-A2. Checkerboard microdilution assay was used to assess the interactions between antifungal and non-antifungal drugs. Compared to antifungal agents, diltiazem and verapamil hydrochloride exerted a relatively low antifungal activity with high minimal inhibitory concentration values (853-2731 μg/ml). Although in combination they could increase the antifungal activity of amphotericin B, itraconazole and voriconazole. Indifferent and synergistic interactions were registered in 33 and 17 cases, respectively. Antagonistic interactions were not revealed between the investigated compounds. However, the observed high MICs suggest that these agents could not be considered as alternative systemic antifungal agents.

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来源期刊
Acta Biologica Hungarica
Acta Biologica Hungarica 生物-生物学
CiteScore
1.40
自引率
0.00%
发文量
0
审稿时长
3 months
期刊介绍: Acta Biologica Hungarica provides a forum for original research works in the field of experimental biology. It covers cytology, functional morphology, embriology, genetics, endocrinology, cellular physiology, plant physiology, neurobiology, ethology and environmental biology with emphasis on toxicology. Publishes book reviews and advertisements.
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