Acta Biologica Hungarica最新文献_第10页

Indoleacetic acid production and plant growth promoting potential of bacterial endophytes isolated from rice (Oryza sativa L.) seeds. 水稻(Oryza sativa L.)种子内生细菌的吲哚乙酸产酸和促植物生长潜力

Acta Biologica Hungarica Pub Date : 2017-06-01 DOI: 10.1556/018.68.2017.2.5

Raheem Shahzad, Muhammad Waqas, Abdul Latif Khan, Khadija Al-Hosni, Sang-Mo Kang, Chang-Woo Seo, In-Jung Lee

{"title":"Indoleacetic acid production and plant growth promoting potential of bacterial endophytes isolated from rice (Oryza sativa L.) seeds.","authors":"Raheem Shahzad, Muhammad Waqas, Abdul Latif Khan, Khadija Al-Hosni, Sang-Mo Kang, Chang-Woo Seo, In-Jung Lee","doi":"10.1556/018.68.2017.2.5","DOIUrl":"https://doi.org/10.1556/018.68.2017.2.5","url":null,"abstract":"Bacterial endophytes from the phyllosphere and rhizosphere have been used to produce bioactive metabolites and to promote plant growth. However, little is known about the endophytes residing in seeds. This study aimed to isolate and identify seed-borne bacterial endophytes from rice and elucidate their potential for phytohormone production and growth enhancement. The isolated endophytes included Micrococcus yunnanensis RWL-2, Micrococcus luteus RWL-3, Enterobacter soli RWL-4, Leclercia adecarboxylata RWL-5, Pantoea dispersa RWL-6, and Staphylococcus epidermidis RWL-7, which were identified using 16S rRNA sequencing and phylogenetic analysis. These strains were analyzed for indoleacetic acid (IAA) production by using GC-MS and IAA was found in the range of 11.50 ± 0.77 μg ml-1 to 38.80 ± 1.35 μg ml-1. We also assessed the strains for plant growth promoting potential because these isolates were able to produce IAA in pure culture. Most of the growth attributes of rice plants (shoot and root length, fresh and dry biomass, and chlorophyll content) were significantly increased by bacterial endophytes compared to the controls. These results show that IAA producing bacterial endophytes can improve hostplant growth traits and can be used as bio-fertilizers.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 2","pages":"175-186"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.2.5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35082030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 63

Hydrogen peroxide and nitric oxide regulation of phenolic metabolism under water stress and ABA in wheat. 过氧化氢和一氧化氮对水分胁迫和ABA下小麦酚代谢的调控。

Acta Biologica Hungarica Pub Date : 2017-06-01 DOI: 10.1556/018.68.2017.2.4

Rattanpreet Kaur, Vikramjit Kaur Zhawar

{"title":"Hydrogen peroxide and nitric oxide regulation of phenolic metabolism under water stress and ABA in wheat.","authors":"Rattanpreet Kaur, Vikramjit Kaur Zhawar","doi":"10.1556/018.68.2017.2.4","DOIUrl":"https://doi.org/10.1556/018.68.2017.2.4","url":null,"abstract":"Wheat cultivar PBW644 (drought tolerant) and PBW343 (drought sensitive) were found as ABA-higher sensitive and ABA-lesser sensitive, respectively, in the screen of six wheat cultivars. Both cultivars were studied for H2O2 (ROS)/nitric oxide (NO)-regulation of growth and phenolic metabolism under ABA and water stress (WS) by supplying ROS/NO producers as well as scavengers. Endogenous ROS/NO under ABA/WS increased growth, such effect was higher in PBW644. In PBW343, reduced growth under WS was improved by exogenous ROS/NO. Exogenous ROS/NO under ABA/WS decreased lignin and increased phenolics in PBW343 but such relation was not found in PBW644. Endogenous NO under WS increased flavonoids in both cultivars. Both ROS/NO under ABA/WS increased flavonoids in PBW644, however, in PBW343, only ROS increased these in roots. Under WS, PBW644 showed higher levels of cell wall peroxidase (CW-POX) and lower levels of soluble peroxidase (S-POX) than PBW343. However, under ABA, it showed higher levels of both peroxidases. ROS/NO signals under ABA increased both types of POX in both cultivars while under WS, these signals increased both types in PBW343 but CW-POX only in PBW644. Polyphenol oxidases were ABA-upregulated in PBW644 only. Under WS, these enzymes were maintained higher in PBW343. This study indicated that tolerant cultivar under WS contained sufficient endogenous ROS/NO signalling to which susceptible cultivar lacked but showed improvement on exogenous applications. Secondly, tolerant cultivar was using less phenolic activity under WS which could be due to the presence of sufficient levels of primary antioxidants.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 2","pages":"162-174"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.2.4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35082031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Isolation and preliminary characterization of a bacteriocin-producer Bacillus strain inhibiting methicillin resistant Staphylococcus aureus. 一株抑制耐甲氧西林金黄色葡萄球菌的产菌素芽孢杆菌的分离及初步鉴定。

Acta Biologica Hungarica Pub Date : 2017-06-01 DOI: 10.1556/018.68.2017.2.8

Ankit Kumar Chauhan, Dinesh Kumar Maheshwari, Vivek K Bajpai

{"title":"Isolation and preliminary characterization of a bacteriocin-producer Bacillus strain inhibiting methicillin resistant Staphylococcus aureus.","authors":"Ankit Kumar Chauhan, Dinesh Kumar Maheshwari, Vivek K Bajpai","doi":"10.1556/018.68.2017.2.8","DOIUrl":"https://doi.org/10.1556/018.68.2017.2.8","url":null,"abstract":"In a multivalent approach to discover new antimicrobial substance, a total of 160 Bacilli were isolated from termitarium soil, characterized on the basis of their morphological and physiological characters and screened for their antimicrobial activity by agar well diffusion method against certain drug resistant pathogenic bacteria such as Staphylococcus aureus, Methicillin resistant Staphylococcus aureus and common food contaminating bacteria Listeria monocytogenes. After preliminary screening, sixteen isolates showed inhibitory activity against test pathogens. Among them Bacillus isolate TSH58 exhibited maximum inhibitory activity against MRSA, Staphylococcus aureus and Listeria monocytogenes. Based on morphological, physiological, biochemical and 16S rDNA characteristics isolate TSH58 was identified as a member of the Bacillus cereus species group. Various nutrient sources and culture conditions were optimized, the partially purified antimicrobial metabolite was subjected to various treatments such as heat, pH and proteolytic enzymes. Complete loss in the activity observed when the crude metabolite was treated with proteolytic enzymes suggesting its proteinaceous nature and termed as bacteriocin like inhibitory substance (BLIS). Minimal inhibitory concentration of the partially purified bacteriocin determined by microtiter plate assay was 80 μg/ml for MRSA and 40 μg/ml for L. monocytogenes. Tricine SDS PAGE analysis revealed that the partially purified bacteriocin produced by the Bacillus strain TSH58 had an apparent molecular weight of about 4.0 KDa.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 2","pages":"208-219"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.2.8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35081594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Chemical composition and antifungal activity of Hedera helix leaf ethanolic extract. 蛇麻叶乙醇提取物的化学成分及抗真菌活性研究。

Acta Biologica Hungarica Pub Date : 2017-06-01 DOI: 10.1556/018.68.2017.2.7

Oana Roşca-Casian, Cristina Mircea, Laurian Vlase, Ana-Maria Gheldiu, Delia Tania Teuca, Marcel Pârvu

{"title":"Chemical composition and antifungal activity of Hedera helix leaf ethanolic extract.","authors":"Oana Roşca-Casian, Cristina Mircea, Laurian Vlase, Ana-Maria Gheldiu, Delia Tania Teuca, Marcel Pârvu","doi":"10.1556/018.68.2017.2.7","DOIUrl":"https://doi.org/10.1556/018.68.2017.2.7","url":null,"abstract":"The 50% ethanol extract obtained from Hedera helix leaves was investigated regarding the presence and quantity of polyphenols, sterols and in vitro antifungal activity against phytopathogenic fungi. The chemical analysis revealed the presence of rutin, quercetin and kaempferol in the non-hydrolysed sample and quercetin and kaempferol in the hydrolysed sample and stigmasterol in the ivy leaf extract (nonhydrolysed sample). The antifungal activity against phytopathogenic fungi (Aspergillus niger, Botrytis cinerea, B. tulipae, Fusarium oxysporum f. sp. tulipae, Penicillium gladioli, and Sclerotinia sclerotiorum) was assessed using an agar dilution assay. The results are expressed as the minimum inhibitory concentration (MIC = 10-14%) and were compared to a synthetic antifungal drug - fluconazole (MIC = 8-30%). This report presents the first screening of the antifungal activity of the ivy leaf extract on these plant pathogenic fungi species, aiming to use the ivy leaf extract for controlling different diseases of vegetables and ornamental plants, in addition to human disorders.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 2","pages":"196-207"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.2.7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35082033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

HPLC method for measurement of human salivary α-amylase inhibition by aqueous plant extracts. 测定植物提取物对人唾液α-淀粉酶抑制作用的高效液相色谱法。

Acta Biologica Hungarica Pub Date : 2017-06-01 DOI: 10.1556/018.68.2017.2.1

István Takács, Ákos Takács, Anikó Pósa, Gyöngyi Gyémánt

{"title":"HPLC method for measurement of human salivary α-amylase inhibition by aqueous plant extracts.","authors":"István Takács, Ákos Takács, Anikó Pósa, Gyöngyi Gyémánt","doi":"10.1556/018.68.2017.2.1","DOIUrl":"https://doi.org/10.1556/018.68.2017.2.1","url":null,"abstract":"Control of hyperglycemia is an important treatment in metabolic disorders such as type II diabetes and obesity. α-Amylase, as the first enzyme of glucose release from dietary polysaccharides, is a potential target to identify new sources of novel anti-obesity and anti-diabetic drugs. In this work, different herbal extracts as α-amylase inhibitors were studied by measuring the rate of the cleavage of a maltooligomer substrate 2-chloro-4-nitrophenyl-β-D-maltoheptoside. Measurement of chromophore containing products after reversed phase HPLC separation was used for α-amylase activity measurement. Rates of hydrolysis catalysed by human salivary α-amylase were determined in the presence and absence of lyophilised water extracts of eleven herbs. Remarkable bioactivities were found for extracts of Cinnamomum zeylanicum Blume (bark), Camellia sinensis L. (leaf), Ribes nigrum L. (leaf), Laurus nobilis L. (leaf), Vaccinium macrocarpon Aiton (fruit) and Syzygium aromaticum L. (bud). Determined IC50 values were in 0.017-41 μg/ml range for these six selected plant extracts. Our results confirm the applicability of this HPLC-based method for the quick and reliable comparison of plants as α-amylase inhibitors.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 2","pages":"127-136"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.2.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35081590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Evaluation of antiproliferative and hepatoprotective effects of wheat grass (Triticum aestivum). 小麦草(Triticum aestivum)抗增殖和肝保护作用的评价。

Acta Biologica Hungarica Pub Date : 2017-06-01 DOI: 10.1556/018.68.2017.2.3

Anand Rajoria, Archana Mehta, Pradeep Mehta, Laxmi Ahirwal, Shruti Shukla, Vivek K Bajpai

{"title":"Evaluation of antiproliferative and hepatoprotective effects of wheat grass (Triticum aestivum).","authors":"Anand Rajoria, Archana Mehta, Pradeep Mehta, Laxmi Ahirwal, Shruti Shukla, Vivek K Bajpai","doi":"10.1556/018.68.2017.2.3","DOIUrl":"https://doi.org/10.1556/018.68.2017.2.3","url":null,"abstract":"This study was aimed to evaluate the pharmacological potential of various extracts (hexane, chloroform, methanol and aqueous) of dried shoots of Triticum aestivum (wheat grass) in terms of antiproliferative and hepatoprotective potential of T. aestivum. The total chlorophyll content in dried shoots of T. aestivum was 0.54 ± 0.016 g/L (chlorophyll-a: 0.288 ± 0.05 g/L; and chlorophyll-b; 0.305 ± 0.05 g/L), while total carotene content was 0.42 ± 0.066 g/L. In addition, the chloroform extract of dried shoots of T. aestivum (250 μg/mL) exhibited 87.23% inhibitory effect with potent cytotoxicity against human hepatocellular carcinoma (HepG2) cancer cell line. Moreover, chloroform and methanol extracts significantly reduced the levels of SGOT, and SGPT enzymes, as well as total bilirubin content, while raised the level of total protein in a concentration-gradient manner, confirming the potent hepatoprotective effect of T. aestivum. A possible mechanism of apoptosis of the chloroform extract of dried shoots of T. aestivum in terms of its potent antiproliferative activity against HepG2 cancer cell line can also be proposed in this study. Our findings clearly demonstrate that T. aestivum has a significant pharmacological potential that night be used for antiproliferative and hepatoprotective purposes.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 2","pages":"150-161"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.2.3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35081591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Linalool-induced oxidative stress processes in the human pathogen Candida albicans. 人病原体白色念珠菌中芳樟醇诱导的氧化应激过程。

Acta Biologica Hungarica Pub Date : 2017-06-01 DOI: 10.1556/018.68.2017.2.9

Gábor Máté, Dominika Kovács, Zoltán Gazdag, Miklós Pesti, Árpád Szántó

{"title":"Linalool-induced oxidative stress processes in the human pathogen Candida albicans.","authors":"Gábor Máté, Dominika Kovács, Zoltán Gazdag, Miklós Pesti, Árpád Szántó","doi":"10.1556/018.68.2017.2.9","DOIUrl":"https://doi.org/10.1556/018.68.2017.2.9","url":null,"abstract":"The present study investigated the linalool (Lol)-induced effects in acute toxicity tests in the human pathogen Candida albicans (C. albicans). Lol treatments induced reduced germ tube formation of the pathogen, which plays a crucial role in the virulence. In comparison with the untreated control, the exposure of 107 cells ml-1 to 0.7 mM or 1.4 mM Lol for one hour induced 20% and 30% decrements, respectively, in the colony-forming ability. At the same time, these treatments caused dose-dependent decrease in the levels of superoxide anion radical and total reactive oxygen species, while there was 1.5 and 1.8-fold increases in the concentrations of peroxides and lipid peroxides, respectively, indicating oxidative stress induction in the presence of Lol. Lol treatments resulted in different adaptive modifications of the antioxidant system. In 0.7 mM-treated cells, decreased specific activities of superoxide dismutase and catalase were detected, while exposure to 1.4 mM Lol resulted in the up-regulation of catalase, glutathione reductase and glutathione peroxidases.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 2","pages":"220-231"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.2.9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35081593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Physiological role of exogenous nitric oxide in improving performance, yield and some biochemical aspects of sunflower plant under zinc stress. 外源一氧化氮在锌胁迫下向日葵植株生产性能、产量及生化方面的生理作用

Acta Biologica Hungarica Pub Date : 2017-03-01 DOI: 10.1556/018.68.2017.1.9

Samia Ageeb Akladious, Heba Ibrahim Mohamed

{"title":"Physiological role of exogenous nitric oxide in improving performance, yield and some biochemical aspects of sunflower plant under zinc stress.","authors":"Samia Ageeb Akladious, Heba Ibrahim Mohamed","doi":"10.1556/018.68.2017.1.9","DOIUrl":"https://doi.org/10.1556/018.68.2017.1.9","url":null,"abstract":"The present study was undertaken to examine the possible roles of sodium nitroprusside in protection against oxidative damage due to zinc toxicity in sunflower plants. Physiochemical parameters in sunflower plants exposed to Zn2+ (100, 200 and 300 mg/kg soil) alone or combined with SNP were measured. The results showed that excess of Zn decreased plant growth, seed yield components and photosynthetic pigments content. On the other hand, Zn stress increased the level of non-enzymatic antioxidants (ascorbic acid and reduced glutathione) and enzymatic antioxidants (superoxide dismutase, ascrobate peroxidase and glutathione reductase), coupled with the appearance of novel protein bands. Furthermore, Zn stress increased Zn content in roots and shoots. The amounts of Zn in roots were higher than shoots. A marked increase in total saturated fatty acids accompanied by a decrease in total unsaturated fatty acids was observed. Exogenously application of SNP (20 μM) increased growth parameters, photosynthetic pigments content, ascorbic acid and glutathione contents, antioxidant enzyme activities and the quality of the oil in favour of the increase of unsaturated fatty acids. Moreover, SNP application increased Zn concentration in roots and inhibited Zn accumulation in shoots. Therefore, it is concluded that SNP treatment can help reduce Zn toxicity in sunflower plants.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 1","pages":"101-114"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.1.9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34837388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Alpha-tocopherol-dependent salt tolerance is more related with auxin synthesis rather than enhancement antioxidant defense in soybean roots. 大豆根系中的α-生育酚依赖性耐盐性更多地与辅助素合成有关，而不是与增强抗氧化防御有关。

Acta Biologica Hungarica Pub Date : 2017-03-01 DOI: 10.1556/018.68.2017.1.10

Seda Sereflioglu, Burcu Seckin Dinler, Eda Tasci

{"title":"Alpha-tocopherol-dependent salt tolerance is more related with auxin synthesis rather than enhancement antioxidant defense in soybean roots.","authors":"Seda Sereflioglu, Burcu Seckin Dinler, Eda Tasci","doi":"10.1556/018.68.2017.1.10","DOIUrl":"10.1556/018.68.2017.1.10","url":null,"abstract":"In this paper, we describe the alleviated effects of alpha-tocopherol (α-T) on oxidative damage and its possible role as a signal transmitter in plants during salt stress. The results show that exogenously applied α-T under salt stress increased root length and weight, but reduced hydrogen peroxide (H2O2), superoxide anion radical (O2.-) and malondialdehyde (MDA) content in soybean roots. The proline content was reduced by α-T treatment. Interestingly, endogenous auxin (IAA) level was significantly increased after α-T application as compared to salt stress alone. Moreover, α-T reduced significantly superoxide dismutase (SOD) enzyme and isoenzyme activity but upregulated peroxidase (POX) 2, 3 and glutathione-s-transferase (GST) 1, 3 isoenzyme expression. However, ascorbate peroxidase (APX) enzyme activity was not affected at all. Consequently, the results show that α-T serves as a signal molecule under salinity from leaves to roots by increasing remarkably endogenous IAA levels and increasing partially antioxidant activity in roots.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 1","pages":"115-125"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.1.10","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34837389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Physico-chemical changes in karkade (Hibiscus sabdariffa L.) seedlings responding to salt stress. 盐胁迫下木槿幼苗的理化变化

Acta Biologica Hungarica Pub Date : 2017-03-01 DOI: 10.1556/018.68.2017.1.7

Abdelnasser Galal

{"title":"Physico-chemical changes in karkade (Hibiscus sabdariffa L.) seedlings responding to salt stress.","authors":"Abdelnasser Galal","doi":"10.1556/018.68.2017.1.7","DOIUrl":"https://doi.org/10.1556/018.68.2017.1.7","url":null,"abstract":"Salinity is one of the major abiotic stress factors affecting series of morphological, physiological, metabolic and molecular changes in plant growth. The effect of different concentrations (0, 25, 50, 100 and 150 mM) of NaCl on the vegetative growth and some physiological parameters of karkade (Hibiscus sabdariffa var. sabdariffa) seedling were investigated. NaCl affected the germination rate, delayed emergence and retarded vegetative growth of seedlings. The length of seedling as well as the leaf area was significantly reduced. The fresh weight remained lower in NaCl treated seedlings compared to control. NaCl at 100 and 150 mM concentrations had significant effect on the dry matter contents of the treated seedlings. The chloroplast pigments in the treated seedlings were affected, suggesting that the NaCl had a significant effect on the chlorophyll and carotenoid biosynthesis. The results showed that the salt treatments induced an increase in proline concentration of the seedlings. The osmotic potential (ψs) of NaCl treated seedlings decreased with increasing NaCl concentrations. Salt treatments resulted in dramatic quantitative reduction in the total sterol percent compared with control ones. Salt stress resulted in increase and decrease of Na+ and K+ ions, respectively. NaCl salinity increased lipid peroxidation. SDS-PAGE was used to evaluate protein pattern after applying salt stress. High molecular weight proteins were intensified, while low molecular weight proteins were faint. NaCl at 100 and 150 mM concentration distinguished with new protein bands. Salt stress induced a new peroxidase bands and increased the band intensity, indicating the protective role of peroxidase enzyme.","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"68 1","pages":"73-87"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2017.1.7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34837794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8