Hydrogen peroxide and nitric oxide regulation of phenolic metabolism under water stress and ABA in wheat.

Abstract

Wheat cultivar PBW644 (drought tolerant) and PBW343 (drought sensitive) were found as ABA-higher sensitive and ABA-lesser sensitive, respectively, in the screen of six wheat cultivars. Both cultivars were studied for H₂O₂ (ROS)/nitric oxide (NO)-regulation of growth and phenolic metabolism under ABA and water stress (WS) by supplying ROS/NO producers as well as scavengers. Endogenous ROS/NO under ABA/WS increased growth, such effect was higher in PBW644. In PBW343, reduced growth under WS was improved by exogenous ROS/NO. Exogenous ROS/NO under ABA/WS decreased lignin and increased phenolics in PBW343 but such relation was not found in PBW644. Endogenous NO under WS increased flavonoids in both cultivars. Both ROS/NO under ABA/WS increased flavonoids in PBW644, however, in PBW343, only ROS increased these in roots. Under WS, PBW644 showed higher levels of cell wall peroxidase (CW-POX) and lower levels of soluble peroxidase (S-POX) than PBW343. However, under ABA, it showed higher levels of both peroxidases. ROS/NO signals under ABA increased both types of POX in both cultivars while under WS, these signals increased both types in PBW343 but CW-POX only in PBW644. Polyphenol oxidases were ABA-upregulated in PBW644 only. Under WS, these enzymes were maintained higher in PBW343. This study indicated that tolerant cultivar under WS contained sufficient endogenous ROS/NO signalling to which susceptible cultivar lacked but showed improvement on exogenous applications. Secondly, tolerant cultivar was using less phenolic activity under WS which could be due to the presence of sufficient levels of primary antioxidants.