Acta Biologica Hungarica最新文献

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Transcriptional profiling of catalase genes in juglone-treated seeds of maize (Zea mays L.) and wheat (Triticum aestivum L.). 核桃素处理的玉米和小麦种子过氧化氢酶基因转录谱分析
Acta Biologica Hungarica Pub Date : 2018-12-01 DOI: 10.1556/018.69.2018.4.7
Hubert Sytykiewicz, Agnieszka Kozak, Bogumił Leszczyński, Cezary Sempruch, Iwona Łukasik, Iwona Sprawka, Katarzyna Kmieć, Monika Kurowska, Aldona Kopczyńska, Paweł Czerniewicz
{"title":"Transcriptional profiling of catalase genes in juglone-treated seeds of maize (Zea mays L.) and wheat (Triticum aestivum L.).","authors":"Hubert Sytykiewicz,&nbsp;Agnieszka Kozak,&nbsp;Bogumił Leszczyński,&nbsp;Cezary Sempruch,&nbsp;Iwona Łukasik,&nbsp;Iwona Sprawka,&nbsp;Katarzyna Kmieć,&nbsp;Monika Kurowska,&nbsp;Aldona Kopczyńska,&nbsp;Paweł Czerniewicz","doi":"10.1556/018.69.2018.4.7","DOIUrl":"https://doi.org/10.1556/018.69.2018.4.7","url":null,"abstract":"<p><p>The major aim of the present study was to investigate the influence of juglone (JU; 5-hydroxy-1,4-naphthoquinone) treatments on the expression level of Cat1, Cat2 and Cat3 genes, encoding the respective catalase isozymes in maize (Zea mays L.) and wheat (Triticum aestivum L.) seeds. In parallel, germination efficiency, catalase (CAT) activity and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content in juglone-exposed cereal seeds were assessed. Juglone applications significantly stimulated abundance of three target catalase transcripts as well as induced CAT activity and generation of H<sub>2</sub>O<sub>2</sub> in both maize and wheat kernels. Furthermore, germination process of juglone-affected maize seeds was more severe suppressed than in case of wheat kernels. The role of juglone in triggering the oxidative stress as well as antioxidative responses in seeds of the studied model cereal species are discussed.</p>","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"69 4","pages":"449-463"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.69.2018.4.7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36804510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The application of Magnetic Resonance Imaging (mri) to the examination of plant tissues and water barriers. 磁共振成像(mri)在植物组织和水屏障检查中的应用。
Acta Biologica Hungarica Pub Date : 2018-12-01 DOI: 10.1556/018.69.2018.4.5
Pál Jakusch, Tímea Kocsis, Ilona Kovácsné Székely, István Gábor Hatvani
{"title":"The application of Magnetic Resonance Imaging (mri) to the examination of plant tissues and water barriers.","authors":"Pál Jakusch,&nbsp;Tímea Kocsis,&nbsp;Ilona Kovácsné Székely,&nbsp;István Gábor Hatvani","doi":"10.1556/018.69.2018.4.5","DOIUrl":"https://doi.org/10.1556/018.69.2018.4.5","url":null,"abstract":"<p><p>The aim of the present study is to extend the applicability of MRI measurements similar to those used in human diagnostics to the examination of water barriers in living plants, thus broadening their use in natural sciences. The cucumber, Cucumis sativus, and Phillyrea angustifolia, or false olive, were chosen as test plants. The MRI measurements were carried out on three samples of each plant in the same position vis-a-vis the MRI apparatus using a Siemens Avanto MRI scanner. Two different relaxation times were employed, T<sub>1</sub>, capable of histological mapping, and T<sub>2</sub>, used for the examination of water content. In the course of the analysis, it was found that certain histological formations and branching cause modifications to the intensity detected with relaxation time T<sub>2</sub>. Furthermore, these positions can also be found in T1 measurements. A monotonic correlation (cucumber: ρ = 0.829; false olive: ρ = -0.84) was observed between the T<sub>1</sub> and T<sub>2</sub> measurements. In the course of the statistical analysis of the signal intensities of the xylems it was concluded that they cannot be regarded as independent in a statistical sense; these changes rather depend on the anatomic structure of the plant, as the intensity profile is modified by nodes, leaves and branches. This serves as a demonstration of the applicability of MRI to the measurement of well know plant physiological processes. The special parametrization required for this equipment, which is usually used in human diagnostics, is also documented in the present study.</p>","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"69 4","pages":"423-436"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.69.2018.4.5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36814804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Biochemical changes in ears of wheat genotypes subjected to Fusarium spp. attack. 镰刀菌侵染小麦基因型穗部生化变化。
Acta Biologica Hungarica Pub Date : 2018-12-01 DOI: 10.1556/018.69.2018.4.10
Tihana Marček, Marija Viljevac Vuletić, Valentina Španić
{"title":"Biochemical changes in ears of wheat genotypes subjected to Fusarium spp. attack.","authors":"Tihana Marček,&nbsp;Marija Viljevac Vuletić,&nbsp;Valentina Španić","doi":"10.1556/018.69.2018.4.10","DOIUrl":"https://doi.org/10.1556/018.69.2018.4.10","url":null,"abstract":"<p><p>In wheat, Fusarium fungus promotes the appearance of destructive disease named as Fusarium head blight (FHB) that can cause grain yield reduction and mycotoxin accumulation. The focus of this research was to verify the influence of Fusarium graminearum and F. culmorum on wheat genotypes with different susceptibility to FHB: \"Super Žitarka\" (susceptible), \"Lucija\" (moderately resistant) and \"Apache\" (resistant). The experiment was performed under field conditions by artificial spore inoculation of ears at the flowering stage. The effectiveness of antioxidative enzymes, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content and malondialdehyde (MDA) content were observed at several sampling points after Fusarium inoculation (3, 15 and 24 hours). \"Lucija\" responded to pathogen by increase of guaiacol peroxidase (POD) activity, high H<sub>2</sub>O<sub>2</sub> and MDA content in the early post-inoculation times (3 and 15 hours), compared to control. \"Super Žitarka\" displayed inhibition of catalase (CAT) activity throughout the whole time course of the experiment. Infected plants of \"Apache\" showed notable decline in MDA content over time. Moreover, in \"Apache\" increased H<sub>2</sub>O<sub>2</sub> accumulation was observed immediately after Fusarium exposure (3 and 15 hours), compared to 24 hours. Rapid overproduction of H<sub>2</sub>O<sub>2</sub> under Fusarium stress marked \"Apache\" as FHB-resistant.</p>","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"69 4","pages":"493-504"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.69.2018.4.10","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36858987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Effects of drought stress on hybrids of Vigna radiata at germination stage. 干旱胁迫对辐射豇豆杂交种萌发期的影响。
Acta Biologica Hungarica Pub Date : 2018-12-01 DOI: 10.1556/018.69.2018.4.9
Shehzadi Saima, Guishuang Li, Guang Wu
{"title":"Effects of drought stress on hybrids of Vigna radiata at germination stage.","authors":"Shehzadi Saima,&nbsp;Guishuang Li,&nbsp;Guang Wu","doi":"10.1556/018.69.2018.4.9","DOIUrl":"https://doi.org/10.1556/018.69.2018.4.9","url":null,"abstract":"<p><p>Drought is one the critical abiotic factors that reduces the germination, growth and yield of crops. Therefore the present project was designed with the objective to screen the best drought tolerant hybrid of Vigna radiata. Genetic variations for drought tolerance among these hybrids were assessed by simple and efficient technique. Seven hybrids of V. radiata (9801, 7002, 9706, 08003, 07007, 97012 and 08007) were used for screening against three levels of drought stress (zero, control), 5% (-0.05 MPa) and 10% (-0.1 MPa) induced by Polyethyleneglycol (PEG6000). A higher proline content was observed in 07007 (6.10 μg/g fresh weight) as compared to all other hybrids. Treated seedlings of each hybrid were compared with their respective control to evaluate the differences in their growth under drought. Different parameters such as percentage of germination, germination stress tolerance index (GSI), shoot and root weights (bath fresh and dry) and lengths, root length stress index (RLSI), dry matter stress index (DMSI) and plant height stress index (PHSI) showed considerable variations. Germination percentage, shoot weights, PHSI and DMSI decreased in all hybrids along with the increase of PEG induced drought stress (5% and 10% PEG). In contrary, root weights and RLSI were increased under drought. Overall 07007 showed a better performance, and can therefore be classified as a drought tolerant hybrid.</p>","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"69 4","pages":"481-492"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.69.2018.4.9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36858988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Inhibition of the formation of amyloid-like fibrils with spices, especially cloves. 用香料,尤指丁香抑制淀粉样原纤维的形成
Acta Biologica Hungarica Pub Date : 2018-12-01 DOI: 10.1556/018.69.2018.4.2
Márta Kotormán, Alexandra Varga, Phanindra Babu Kasi, János Nemcsók
{"title":"Inhibition of the formation of amyloid-like fibrils with spices, especially cloves.","authors":"Márta Kotormán,&nbsp;Alexandra Varga,&nbsp;Phanindra Babu Kasi,&nbsp;János Nemcsók","doi":"10.1556/018.69.2018.4.2","DOIUrl":"https://doi.org/10.1556/018.69.2018.4.2","url":null,"abstract":"<p><p>During the study of inhibition of amyloid fibril formation, α-chymotrypsin protein was developed in 55% ethanol at pH 7.0. We investigated the inhibitory effect of different spices on amyloid fibril formation using turbidity measurements and Congo red binding assays. We found that all spices except the black pepper and caraway seed prevented fibril formation. The highest inhibition was measured with the clove, which reduced the amount of aggregates by 90%. We studied the inhibitory effect of the cloves at different concentrations on aggregation, it was found that the inhibitory activity of clove is dependent on concentration. We have measured the total phenolic content of the spice extracts too. Based on all these findings we have come to the following conclusion: Our results indicate that spices can contain other compounds too - not only phenolic compounds - which influence the formation of amyloid fibrils, and the effectiveness of various phenolic compounds are different.</p>","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"69 4","pages":"385-394"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.69.2018.4.2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36858989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models. 靶向抗癌药物对胚胎斑马鱼和海胆模型的毒性比较评价。
Acta Biologica Hungarica Pub Date : 2018-12-01 DOI: 10.1556/018.69.2018.4.3
Tamara Babic, Jelena Dinic, Sonja Stojkovic Buric, Stefan Hadzic, Milica Pesic, Dragica Radojkovic, Aleksandra Divac Rankov
{"title":"Comparative toxicity evaluation of targeted anticancer therapeutics in embryonic zebrafish and sea urchin models.","authors":"Tamara Babic,&nbsp;Jelena Dinic,&nbsp;Sonja Stojkovic Buric,&nbsp;Stefan Hadzic,&nbsp;Milica Pesic,&nbsp;Dragica Radojkovic,&nbsp;Aleksandra Divac Rankov","doi":"10.1556/018.69.2018.4.3","DOIUrl":"https://doi.org/10.1556/018.69.2018.4.3","url":null,"abstract":"<p><p>Cancer drug resistance and poor selectivity towards cancer cells demand the constant search for new therapeutics. PI3K-Akt-mTOR and RAS-MAPK-ERK signaling pathways are key mechanisms involved in cell survival, proliferation, differentiation, and metabolism and their deregulation in cancer can promote development of therapy resistance. We investigated the effects of targeted inhibitors (wortmannin, GSK690693, AZD2014 and tipifarnib) towards these two pathways on early zebrafish and sea urchin development to assess their toxicity in normal, fast proliferating cells. PI3K inhibitor wortmannin and RAS inhibitor tipifarnib displayed highest toxicity while GSK690693, a pan-Akt kinase inhibitor, exhibited a less significant impact on embryo survival and development. Moreover, inhibition of the upstream part of the PI3K-Akt-mTOR pathway (wortmannin/GSK690693 co-treatment) produced a synergistic effect and impacted zebrafish embryo survival and development at much lower concentrations. Dual mTORC1/mTORC2 inhibitor AZD2014 showed no considerable effects on embryonic cells of zebrafish in concentrations substantially toxic in cancer cells. AZD2014 also caused the least prominent effects on sea urchin embryo development compared to other inhibitors. Significant toxicity of AZD2014 in human cancer cells, its capacity to sensitize resistant cancers, lower antiproliferative activity against human normal cell lines and fast proliferating embryonic cells could make this agent a promising candidate for anticancer therapy.</p>","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"69 4","pages":"395-410"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.69.2018.4.3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36858990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
ABA regulation of antioxidant activity during post-germination desiccation and subsequent rehydration in wheat. ABA对小麦萌发后脱水和再水化过程中抗氧化活性的调控。
Acta Biologica Hungarica Pub Date : 2018-09-01 DOI: 10.1556/018.68.2018.3.5
Satinder Kour, Vikramjit Kaur Zhawar
{"title":"ABA regulation of antioxidant activity during post-germination desiccation and subsequent rehydration in wheat.","authors":"Satinder Kour,&nbsp;Vikramjit Kaur Zhawar","doi":"10.1556/018.68.2018.3.5","DOIUrl":"https://doi.org/10.1556/018.68.2018.3.5","url":null,"abstract":"<p><p>ABA regulation of antioxidant activity during post-germination desiccation and subsequent rehydration was studied in two wheat cultivars PBW 644 (ABA-higher sensitive and drought tolerant) and PBW 343 (ABA-lesser sensitive and drought susceptible) where 1 d-germinated seeds were exposed to ABA/ PEG- 6000 for next 1 d, desiccated for 4 d and subsequently rehydrated for 4 d. Ascorbate, dehydrascorbate to ascorbate ratio, malondialdehyde (MDA), hydroxyl radicals, and activities of monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), alcohol dehydrogenase (AlcDH) and aldehyde dehydrogenase (AldDH) were measured in seedlings just before desiccation (2 d old), desiccated (6 d old) and rehydrated (10 d old) stages. ROS/NO signaling was studied under CT and ABA supply by supplying ROS and NO scavengers. During desiccation, both cultivars showed increase of oxidative stress (dehydroascorbate to ascorbate ratio, MDA, hydroxyl radicals) and antioxidant activity in the form of ascorbate content and AldDH activity while other antioxidant enzymes were not increased. PBW 644 showed higher antioxidant activity thus produced less oxidative stress compared to PBW 343. During rehydration, activities of all antioxidant enzymes and levels of ROS (hydroxyl radicals) were increased in both cultivars and MDA was decreased in PBW 343. ABA supply improved desiccation as well as rehydration by improving all parameters of antioxidant activity tested in this study. PEG supply resembled to ABA-supply for its effects. ABA/PEG improvements were seen higher in PBW 644. ROS/NO-signalling was involved under CT as well as under ABA for increasing antioxidant activity during desiccation as well as rehydration in both cultivars.</p>","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"69 3","pages":"283-299"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2018.3.5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36524472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Corchorus olitorius and Urtica pilulifera extracts alleviate copper induced oxidative damage and genotoxicity in tomato. 山茱萸和荨麻提取物可减轻铜诱导的番茄氧化损伤和遗传毒性。
Acta Biologica Hungarica Pub Date : 2018-09-01 DOI: 10.1556/018.68.2018.3.6
Özlem Darcansoy İşeri, Dİdem Aksoy Körpe, Feride Iffet Sahin, Mehmet Haberal
{"title":"Corchorus olitorius and Urtica pilulifera extracts alleviate copper induced oxidative damage and genotoxicity in tomato.","authors":"Özlem Darcansoy İşeri,&nbsp;Dİdem Aksoy Körpe,&nbsp;Feride Iffet Sahin,&nbsp;Mehmet Haberal","doi":"10.1556/018.68.2018.3.6","DOIUrl":"https://doi.org/10.1556/018.68.2018.3.6","url":null,"abstract":"<p><p>Copper cause oxidative damage in plant cells, and plant extracts are the sources of free radical scavengers. We tested the hypothesis that whether Corchorus olitorius (jute) and Urtica pilulifera (Roman nettle) seed extract treatments of germinated seeds affect copper induced oxidative and genotoxic damage or antioxidant response in tomato. Seedlings were exposed to toxic copper concentration (30 ppm) for 7 days. In one experimental group (treatment 1), extract (100 μg mL<sup>-1</sup>) was added to media. In the other group (treatment 2), tomato seeds were pre-soaked by the extract (100 μg mL<sup>-1</sup>) prior to germination and copper application. Malondialdehyde and endogenous H<sub>2</sub>O<sub>2</sub> levels in the groups treated with extract and copper were significantly lower than that of the untreated groups. Pre-soaking seeds with the nettle extract solution significantly enhanced catalase activity under unstressed condition. Jute treatment also enhanced catalase activity under copper stress. Ascorbate peroxidase activity remained at unstressed level in copper treated groups. Extract treatments significantly decreased copper induced DNA damage in root nuclei. Jute seed extract contained salicylic acid and quercetin which can be correlated with the evoked effects. We demonstrated protective effect of plant extract treatments against copper stress of tomato seedlings prior to germination or during seedling development.</p>","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"69 3","pages":"300-312"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2018.3.6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36527881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Isolation and identification of a growth inhibitory substance from Heliotropium indicum L. 从 Heliotropium indicum L. 中分离和鉴定一种生长抑制物质
Acta Biologica Hungarica Pub Date : 2018-09-01 DOI: 10.1556/018.68.2018.3.3
Sirinapa Chaipon, Prapaipit Suwitchayanon, Arihiro Iwasaki, Kiyotake Suenaga, Hisashi Kato-Noguchi
{"title":"Isolation and identification of a growth inhibitory substance from Heliotropium indicum L.","authors":"Sirinapa Chaipon, Prapaipit Suwitchayanon, Arihiro Iwasaki, Kiyotake Suenaga, Hisashi Kato-Noguchi","doi":"10.1556/018.68.2018.3.3","DOIUrl":"10.1556/018.68.2018.3.3","url":null,"abstract":"<p><p>Heliotropium indicum L. belongs to the family Boraginaceae. The plant has been used as a folk medicine because it contains substances of various biological activities. It is also identified as a common weed which grows wildly in crop fields in tropical and subtropical regions of the world. However, there is little information on the allelopathic effect in this plant. Therefore, this study was undertaken to investigate the growth inhibitory effect and to identify the growth inhibitory substances in H. indicum. An aqueous methanol extract of H. indicum inhibited shoot and root growth of barnyard grass, foxtail fescue, timothy, cress, lettuce and rapeseed at concentrations higher than 10 mg dry weight equivalent extract/mL. The concentrations required for 50% growth inhibition (I<sub>50</sub>) of those test plants ranged from 3-282 mg dry weight equivalent extract/mL. The extract was then separated using a sequence of chromatographic fractionations and a growth inhibitory substance was isolated and identified by spectral analysis as methyl caffeate. Methyl caffeate inhibited the growth of lettuce and foxtail fescue at concentrations higher than 1.0 mM. The results suggest that methyl caffeate may contribute to the growth inhibitory effect of H. indicum and may play an important role in the allelopathic effect of H. indicum.</p>","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"69 3","pages":"259-269"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2018.3.3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36527930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
GmLEA2-1, a late embryogenesis abundant protein gene isolated from soybean (Glycine max (L.) Merr.), confers tolerance to abiotic stress. GmLEA2-1 是一个从大豆(Glycine max (L.) Merr.)中分离出来的胚胎发生后期丰富蛋白基因,它能赋予大豆对非生物胁迫的耐受性。
Acta Biologica Hungarica Pub Date : 2018-09-01 DOI: 10.1556/018.68.2018.3.4
Zhikun Wang, Qiang Yang, Yupeng Shao, Binbin Zhang, Aiyun Feng, Fanli Meng, Wenbin Li
{"title":"GmLEA2-1, a late embryogenesis abundant protein gene isolated from soybean (Glycine max (L.) Merr.), confers tolerance to abiotic stress.","authors":"Zhikun Wang, Qiang Yang, Yupeng Shao, Binbin Zhang, Aiyun Feng, Fanli Meng, Wenbin Li","doi":"10.1556/018.68.2018.3.4","DOIUrl":"10.1556/018.68.2018.3.4","url":null,"abstract":"<p><p>Late embryonic proteins (LEA) gene family was abundant mainly in higher plant embryos, which could protect the embryos from the damage caused by abiotic stress, especially drought and salt stresses. In the present study, GmLEA2-1 was cloned from soybean leaf tissue treated by 10% polyethylene glycol 6000 (PEG6000). The results of quantitative real-time PCR (qRT-PCR) revealed a variety of expression patterns of GmLEA2-1 in various tissues of soybean (root, stem, leaf, flower, pod, early embryo and late embryo). GmLEA2-1 gene shared a lower sequence similarity with other typical LEA genes of same group from different species, but similar functions. Overexpression of GmLEA2-1 in transgenic Arabidopsis thaliana conferred tolerance to drought and salt stresses. The fresh weight and dry weight of seedling, the primary root length and the lateral root density of transgenic Arabidopsis plants were higher than those of wild type Arabidopsis (WT) under drought and salt stresses. Cis-acting regulatory elements in the GmLEA2-1 promoter were also predicted. These data demonstrate that GmLEA2-1 protein play an important role in improving drought and salt tolerance in plants.</p>","PeriodicalId":7009,"journal":{"name":"Acta Biologica Hungarica","volume":"69 3","pages":"270-282"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/018.68.2018.3.4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36527928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
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