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Acta histochemica. Supplementband最新文献

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[Proliferation kinetics in rat urothelium after unilateral nephrectomy and various doses of dibutylnitrosamine application--an example of the roles of a one kidney state on carcinogenesis]. [单侧肾切除术和不同剂量二丁基亚硝胺应用后大鼠尿路上皮的增殖动力学——单肾状态在致癌作用中的一个例子]。
Acta histochemica. Supplementband Pub Date : 1990-01-01
F Theuring, C Balint, W Meier
{"title":"[Proliferation kinetics in rat urothelium after unilateral nephrectomy and various doses of dibutylnitrosamine application--an example of the roles of a one kidney state on carcinogenesis].","authors":"F Theuring,&nbsp;C Balint,&nbsp;W Meier","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We investigated the proliferation of urothel of the rat after unilateral nephrectomy and application of DBN with drinking water in various doses. We controlled the measurement of proliferation with 3H-thymidine. The important results of our experiments with DBN-application and unilateral nephrectomy were: 1. the tumour-incidence is higher and 2. the tumour-appear is earlier than in experiments with only DBN.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"39 ","pages":"267-72"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13235541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Origin of islets of Langerhans by mitotic division of duct epithelium in the human early fetal period]. [人早期胎儿时期导管上皮有丝分裂形成朗格汉斯胰岛]。
Acta histochemica. Supplementband Pub Date : 1990-01-01
H Hohn von Dorsche, K Zimmermann, K Fält
{"title":"[Origin of islets of Langerhans by mitotic division of duct epithelium in the human early fetal period].","authors":"H Hohn von Dorsche,&nbsp;K Zimmermann,&nbsp;K Fält","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The investigations are carried out in 29 human fetal pancreases between the 10th and 19th week of age. Mitosis restricted to the duct epithelium in the 10th to 13th week. In the 14th week start the mitotic activity in the islet parenchyma. The mitotic activity decreased from the 10th to the 13th week, increased to the 15th week and decreased once more to the 19th week.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"39 ","pages":"285-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13235543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Importance of the interaction of surface receptors with the cytoskeleton for platelet function]. [表面受体与细胞骨架相互作用对血小板功能的重要性]。
Acta histochemica. Supplementband Pub Date : 1990-01-01
M Meyer
{"title":"[Importance of the interaction of surface receptors with the cytoskeleton for platelet function].","authors":"M Meyer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two Triton-X-100-insoluble cytoskeletal fractions were prepared from washed human blood platelets by centrifugation: a low speed sediment (TP) and a fraction recovered as ultracentrifugation pellet (UP). Glycoproteins associated with both fractions prior to and after different times of thrombin activation were analyzed by electrophoretic techniques. In the TP fraction of unstimulated platelet the major membrane glycoproteins (GP) Ib and Ia were detected. After thrombin stimulation there is a rapid increase of GPs Ia, Ib, IIIb and a Mr 250,000-GP and a slower appearance of GPs IIb and IIIa in this fraction. The UP fraction contains mainly GPs Ib, Ia, IIb, IIIa and a Mr 250,000-GP. Thrombin activation does not cause any significant changes in the glycopeptide pattern of this fraction.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"39 ","pages":"319-25"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13236089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Increase in the cytoskeleton of endothelial cells in mesenteric microvessels in experimental traumatic hemorrhagic shock]. [实验性外伤性失血性休克患者肠系膜微血管内皮细胞骨架增加]。
Acta histochemica. Supplementband Pub Date : 1990-01-01
K Kretschmar, P A Triebke
{"title":"[Increase in the cytoskeleton of endothelial cells in mesenteric microvessels in experimental traumatic hemorrhagic shock].","authors":"K Kretschmar,&nbsp;P A Triebke","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Traumatic haemorrhagic shock in male rats caused an increase of cytoskeletal, especially filaments, in the numerous endothelial cells of the mesenteric microvessels during an examination period of 15 minutes to 2 hours. The filaments become ultrastructurally visible especially in endothelial cells with an enhanced \"activity\", in case of attachment of cells and in narrow capillaries with stasis. Additional investigations of these findings with other results will be carried out.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"39 ","pages":"419-22"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13236096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Purification kinetics of human tumors measured by flow cytometry]. [用流式细胞术测定人肿瘤的纯化动力学]。
Acta histochemica. Supplementband Pub Date : 1990-01-01
K Goerttler
{"title":"[Purification kinetics of human tumors measured by flow cytometry].","authors":"K Goerttler","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We report on the use one-parameter DNA flow cytometry in the evaluation of the proliferation kinetics of malignant tumors. We emphasize the need of a background correction of the histograms in cases where the S-phase portion is used as a prognostic parameter for patients with malignant tumors. Flow cytometric investigations during the last 12 years with many thousand samples of human malignant tumors have shown that the described methodology can be used routinely as \"assistant diagnosis\" for a distinct group of tumors. These tumors comprise carcinomas of the breast and the ovaries and probably also of the endometrium. Regarding the relevance of the method for oropharyngeal squamous carcinomas, gliomas and meningiomas we have to await longterm results. In contrast, at present the method does not seem to be applicable for colorectal carcinomas and it is apparently not suitable for stomach carcinomas. It is also questionable whether data on proliferation kinetics gained in human tumors transplanted to nude mice can be as a basis in chemotherapeutic treatment of patients from whom the tumor originated.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"39 ","pages":"49-60"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13236716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of gold labeled antibodies by video microscopy. 视频显微镜检测金标记抗体。
Acta histochemica. Supplementband Pub Date : 1990-01-01
M Spiess, T Bächi
{"title":"Detection of gold labeled antibodies by video microscopy.","authors":"M Spiess,&nbsp;T Bächi","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"40 ","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13246069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DNA and sex chromatin content in nuclei of conductive system and working myocytes of normal and hypertrophied human heart. 正常和肥大心脏传导系统细胞核及工作心肌细胞DNA和性染色质含量的研究。
Acta histochemica. Supplementband Pub Date : 1990-01-01
P P Rumyantsev, I L Erokhina, E M Antipanova, M G Martynova
{"title":"DNA and sex chromatin content in nuclei of conductive system and working myocytes of normal and hypertrophied human heart.","authors":"P P Rumyantsev,&nbsp;I L Erokhina,&nbsp;E M Antipanova,&nbsp;M G Martynova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>DNA content was measured by means of Feulgen cytophotometry in myocyte nuclei of sinoatrial und atrioventricular nodes, Purkinje cells, left and right atria and ventricles from normal and hypertrophied human hearts both on 12 microns sections and on squash preparations of myocardial tissue. In contrast to ordinary atrial and ventricular myocytes of normal and hypertrophied adult hearts, which contain mostly polyploid nuclei, the DNA content in 74 to 88.5% of myocyte nuclei from sinoatrial and atrioventricular nodes does not exceed 2 c. Unlike nodal myocytes in Purkinje cells of hypertrophied adult heart there are 97 to 99.5% of polyploid nuclei, 49 to 88% of them displaying octaploid and higher DNA values. Myocytes of highly hypertrophied atria contain nuclei of considerably higher ploidy level than those of normal atria. Percentage of binucleated cells show a 4-fold increase in hypertrophied atria compared with normal ones. The total area of nucleoli per nucleus is proportional to the degree of ploidy. Number of sex chromatin bodies counted simultaneously with DNA measurements on sections of atrioventricular node and on squash preparations of atria and ventricles is correlated with ploidy level. Judging by correlations between the number of sex chromatin bodies and DNA content in nuclei, on one hand, and between the total area of nucleoli per nucleus and the degree of ploidy, on the other, the mitotic endoreduplication (true polyploidization) is responsible for the increased DNA content in nuclei of conductive system, atrium and ventricle.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"39 ","pages":"225-37"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13304220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[SEM studies on IFN-receptor dynamics of cultured cells from mammals]. [哺乳动物培养细胞中ifn受体动态的扫描电镜研究]。
Acta histochemica. Supplementband Pub Date : 1990-01-01
K Augsten, E Unger, M Tonew, L Wollweber, F Noll
{"title":"[SEM studies on IFN-receptor dynamics of cultured cells from mammals].","authors":"K Augsten,&nbsp;E Unger,&nbsp;M Tonew,&nbsp;L Wollweber,&nbsp;F Noll","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Visualization of rIFN-alpha 1 and -alpha 2 receptors on MDBK-cells was done by means of a two step method with gold-labelled monoclonal anti IFN-antibodies in the SEM. This binding showed a marked dependency from the temperature, concentration of the rHu-IFN, prefixation of the MDBK-cells, and time of incubation. This results are in agreement with findings of classical virological methods. By means of SEM was shown that the distribution and amount of the IFN-receptors on MDBK-cells depends strongly on the cell cycle and that not each was labelled. The results were discussed in connection with structural-functional alterations of the cytoskeleton.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"39 ","pages":"311-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13304221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The influence of embedding on the stoichiometry of the pararosaniline-Feulgen stain in histological material. 包埋对组织材料中副氨基苯胺- feulgen染色化学计量的影响。
Acta histochemica. Supplementband Pub Date : 1990-01-01
E Schulte
{"title":"The influence of embedding on the stoichiometry of the pararosaniline-Feulgen stain in histological material.","authors":"E Schulte","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the present study the influence of the embedding technique on the dye-substrate-interaction of the Feulgen reagent has been investigated. Pieces of rat liver and of human lung tumours were fixed in formaline, Carnoy's or Bouin's solution or in SuSa and embedded in paraffin (P) or in glycolmethacrylate (GMA). Sections were stained with the pararosaniline-Feulgen-reagent. Mean optical density (MOD) of cell nuclei was measured with an image analyzer. GMA required longer times for hydrolysis and staining than P. the plateau phase of acid hydrolysis was longer in GMA. In general MOD was significantly lower in GMA than in P. The ratio of MOD of lymphocyte nuclei versus diploid basal cell nuclei in lung tissue was 0.97 in GMA and 0.88 in P, respectively. We presume that cross-linking of GMA monomers during polymerization stabilizes hydrolysed DNA. Steric factors are responsible for the slow diffusion of the dye-molecule. The influence of the embedding medium on the stoichiometry of the Feulgen stain has to be carefully considered for statistical evaluation of DNA-histograms.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"38 ","pages":"255-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12871652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Use of a sialic acid analogue to analyze the importance of the receptor-destroying enzyme for the interaction of influenza C virus with cells. 利用硅酸类似物分析受体破坏酶对丙型流感病毒与细胞相互作用的重要性。
Acta histochemica. Supplementband Pub Date : 1990-01-01
G Herrler, H J Gross, G Milks, J C Paulson, H D Klenk, R Brossmer
{"title":"Use of a sialic acid analogue to analyze the importance of the receptor-destroying enzyme for the interaction of influenza C virus with cells.","authors":"G Herrler, H J Gross, G Milks, J C Paulson, H D Klenk, R Brossmer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Influenza C virus uses 9-O-acetyl-N-acetylaneuraminic acid (9-O-acetyl-Neu5Ac) as a receptor determinant for attachment to cells. The virus contains an acetylesterase which releases acetyl residues from position C-9 of sialic acid thereby inactivating the receptors. A synthetic sialic acid analogue, 9-N-acetyl-Neu5Ac, was attached to cell surface glycoconjugates by purified sialyltransferase and analyzed for its ability to substitute the 9-O-acetylated sialic acid. Erythrocytes which have been modified to contain either 9-O-acetyl-Neu5Ac or 9-N-acetyl-Neu5Ac were agglutinated by influenza C virus to the same titer. However, in contrast to the 9-O-acetyl group the 9-N-acetyl residue is resistant to cleavage by the viral acetylesterase. This characteristic property (recognition as a receptor determinant by influenza C virus, but resistance against the action of the receptor-destroying enzyme) makes this synthetic analogue a valuable tool to analyze the role of the receptor-destroying enzyme for an influenza C virus infection.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"40 ","pages":"39-41"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13124762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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