Acta Cirúrgica Brasileira最新文献_第4页

Effects of systemic inflammation due to hepatic ischemia-reperfusion injury upon lean or obese visceral adipose tissue 肝脏缺血再灌注损伤引起的全身性炎症对瘦或肥胖内脏脂肪组织的影响

Acta Cirúrgica Brasileira Pub Date : 2022-03-14 DOI: 10.1590/acb370105

Ligia Fernanda Ferraz, C. R. P. Caria, R. D. C. Santos, M. Ribeiro, A. Gambero

{"title":"Effects of systemic inflammation due to hepatic ischemia-reperfusion injury upon lean or obese visceral adipose tissue","authors":"Ligia Fernanda Ferraz, C. R. P. Caria, R. D. C. Santos, M. Ribeiro, A. Gambero","doi":"10.1590/acb370105","DOIUrl":"https://doi.org/10.1590/acb370105","url":null,"abstract":"ABSTRACT Purpose: To evaluate how the induction of liver damage by ischemia and reperfusion affects the adipose tissue of lean and obese mice. Methods: Lean and diet-induced obese mice were subjected to liver ischemia (30 min) followed by 6 h of reperfusion. The vascular stromal fraction of visceral adipose tissue was analyzed by cytometry, and gene expression was evaluated by an Array assay and by RT-qPCR. Intestinal permeability was assessed by oral administration of fluorescein isothiocyanate (FITC)-dextran and endotoxemia by serum endotoxin measurements using a limulus amebocyte lysate assay. Results: It was found that, after liver ischemia and reperfusion, there is an infiltration of neutrophils, monocytes, and lymphocytes, as well as an increase in the gene expression that encode cytokines, chemokines and their receptors in the visceral adipose tissue of lean mice. This inflammatory response was associated with the presence of endotoxemia in lean mice. However, these changes were not observed in the visceral adipose tissue of obese mice. Conclusions: Liver ischemia and reperfusion induce an acute inflammatory response in adipose tissue of lean mice characterized by an intense chemokine induction and leukocyte infiltration; however, inflammatory alterations are already present at baseline in the obese adipose tissue and liver ischemia and reperfusion do not injure further.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76808311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental model of portal hypertension and esophagogastric varices in minipigs: pressure and endoscopic pilot study 小型猪门脉高压和食管胃静脉曲张的实验模型:压力和内镜初步研究

Acta Cirúrgica Brasileira Pub Date : 2022-03-07 DOI: 10.1590/acb370103

F. Maluf-Filho, A. Meyer, Pierre Pirchner Mathias Martins, F. Galvão, L. D’ALBUQUERQUE

{"title":"Experimental model of portal hypertension and esophagogastric varices in minipigs: pressure and endoscopic pilot study","authors":"F. Maluf-Filho, A. Meyer, Pierre Pirchner Mathias Martins, F. Galvão, L. D’ALBUQUERQUE","doi":"10.1590/acb370103","DOIUrl":"https://doi.org/10.1590/acb370103","url":null,"abstract":"ABSTRACT Introduction: Portal hypertension still represents an important health problem worldwide. In the search for knowledge regarding this syndrome, experimental studies with animal models have proven to be useful to point the direction to be taken in future randomized clinical trials. Purpose: To validate the experimental model of portal hypertension and esophagogastric varices in a medium-sized animal. Methods: This study included five minipigs br1. Midline laparotomy with dissection of the portal vein and production of a calibrated stenosis of this vein was performed. Measurement of pressure in the portal venous and digestive endoscopic were performed before and five weeks after the production of a stenosis. Results: All animals were 8 months old, average weight of 17 ± 2.5 kg. The mean pressure of the portal vein immediately before the partial ligation of the portal vein was 8.9 + 1.6 mm Hg, with 26.6 + 5.4 mm Hg in the second measurement five weeks later (p < 0.05). No gastroesophageal varices or hypertensive portal gastropathy were seen at endoscopy procedures in our sample at any time in the study. Conclusion: Portal vein ligation in minipigs has been validated in the production of portal hypertension, but not in the formation of esophageal varices.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91182509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Octreotide-mediated neurofunctional recovery in rats following traumatic brain injury. Role of H2S, Nrf2 and TNF-α 奥曲肽介导的创伤性脑损伤大鼠神经功能恢复。H2S、Nrf2和TNF-α的作用

Acta Cirúrgica Brasileira Pub Date : 2022-02-23 DOI: 10.1590/ACB361204

Jiegang Zhou, Li Cao, Xia Feng, Baosheng Zhou, Linshan Li

{"title":"Octreotide-mediated neurofunctional recovery in rats following traumatic brain injury. Role of H2S, Nrf2 and TNF-α","authors":"Jiegang Zhou, Li Cao, Xia Feng, Baosheng Zhou, Linshan Li","doi":"10.1590/ACB361204","DOIUrl":"https://doi.org/10.1590/ACB361204","url":null,"abstract":"ABSTRACT Purpose: To explore the role and mechanisms of octreotide in neurofunctional recovery in the traumatic brain injury (TBI) model. Methods: Rats were subjected to midline incision followed by TBI in the prefrontal cortex region. After 72 hours, the behavioural and neurological deficits tests were performed, which included memory testing on Morris water maze for 5 days. Octreotide (15 and 30 mg/kg i.p.) was administered 30 minutes before subjecting to TBI, and its administration was continued for three days. Results: In TBI-subjected rats, administration of octreotide restored on day 4 escape latency time (ELT) and increased the time spent in the target quadrant (TSTQ) on day 5, suggesting the improvement in learning and memory. It also increased the expression of H2S, Nrf2, and cystathionine-γ-lyase (CSE) in the prefrontal cortex, without any significant effect on cystathionine-β-synthase. Octreotide also decreased the TNF-α levels and neurological severity score. However, co-administration of CSE inhibitor (D,L-propargylglycine) abolished octreotide-mediated neurofunctional recovery, decreased the levels of H2S and Nrf2 and increased the levels of TNF-α. Conclusions: Octreotide improved the neurological functions in TBI-subjected rats, which may be due to up-regulation of H2S biosynthetic enzyme (CSE), levels of H2S and Nrf2 and down-regulation of neuroinflammation.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90107066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Hypercapnia attenuates ventilator-induced lung injury through vagus nerve activation 1 高碳酸血症通过迷走神经激活减轻呼吸机诱导的肺损伤1

Acta Cirúrgica Brasileira Pub Date : 2019-11-25 DOI: 10.1590/s0102-865020190090000002

W. Xia, Guang Li, Zhou Pan, Qingshan Zhou

{"title":"Hypercapnia attenuates ventilator-induced lung injury through vagus nerve activation 1","authors":"W. Xia, Guang Li, Zhou Pan, Qingshan Zhou","doi":"10.1590/s0102-865020190090000002","DOIUrl":"https://doi.org/10.1590/s0102-865020190090000002","url":null,"abstract":"Abstract Purpose: To investigate the role of vagus nerve activation in the protective effects of hypercapnia in ventilator-induced lung injury (VILI) rats. Methods: Male Sprague-Dawley rats were randomized to either high-tidal volume or low-tidal volume ventilation (control) and monitored for 4h. The high-tidal volume group was further divided into either a vagotomy or sham-operated group and each surgery group was further divided into two subgroups: normocapnia and hypercapnia. Injuries were assessed hourly through hemodynamics, respiratory mechanics and gas exchange. Protein concentration, cell count and cytokines (TNF-α and IL-8) in bronchoalveolar lavage fluid (BALF), lung wet-to-dry weight and pathological changes were examined. Vagus nerve activity was recorded for 1h. Results: Compared to the control group, injurious ventilation resulted in a decrease in PaO2/FiO2 and greater lung static compliance, MPO activity, enhanced BALF cytokines, protein concentration, cell count, and histology injury score. Conversely, hypercapnia significantly improved VILI by decreasing the above injury parameters. However, vagotomy abolished the protective effect of hypercapnia on VILI. In addition, hypercapnia enhanced efferent vagus nerve activity compared to normocapnia. Conclusion: These results indicate that the vagus nerve plays an important role in mediating the anti-inflammatory effect of hypercapnia on VILI.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90108840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mesenteric lymph drainage alleviates hemorrhagic shock-induced spleen injury and inflammation 1 肠系膜淋巴引流减轻失血性休克引起的脾脏损伤和炎症1

Acta Cirúrgica Brasileira Pub Date : 2019-11-25 DOI: 10.1590/s0102-865020190090000003

Hong Zhang, Jia-Yi Zhai, Hui-Bo Du, Li-Min Zhang, Lin-Feng Li, An-Qi Bian, Li-Na Jiang, Zigang Zhao

{"title":"Mesenteric lymph drainage alleviates hemorrhagic shock-induced spleen injury and inflammation 1","authors":"Hong Zhang, Jia-Yi Zhai, Hui-Bo Du, Li-Min Zhang, Lin-Feng Li, An-Qi Bian, Li-Na Jiang, Zigang Zhao","doi":"10.1590/s0102-865020190090000003","DOIUrl":"https://doi.org/10.1590/s0102-865020190090000003","url":null,"abstract":"Abstract Purpose: To investigate the effect of mesenteric lymph drainage on the spleen injury and the expressions of inflammatory cytokines in splenic tissue in mice following hemorrhagic shock. Methods: Male C57 mice were randomly divided into the sham shock, shock and shock+drainage groups. The mice in both shock and shock+drainage groups suffered femoral artery bleeding, maintained mean arterial pressure (MAP) of 40±2 mmHg for 90 min, and were resuscitated. And mesenteric lymph drainage was performed in the shock+drainage group at the time of resuscitation. After three hours of resuscitation, the splenic tissues were harvested for the histological observation and protein and mRNA expression analysis of cytokines. Results: The spleen in the shock group revealed a significantly structural damage and increased mRNA expressions of MyD88 and TRAF6 and protein expressions of TIPE2, MyD88, TRIF and TRAF3 compared to the sham group. By contrast, the splenic pathological injury in the shock+drainage group was alleviated significantly, and the mRNA and protein expressions of TIPE2, MyD88, TRIF, TRAF3 and TRAF6 were significantly lower than those in the shock group. Conclusion: These results indicate that post-hemorrhagic shock mesenteric lymph drainage alleviates hemorrhagic shock-induced spleen injury and the expressions of inflammatory cytokines.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89456558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Ganoderma lucidum, a promising agent possessing antioxidant and anti-inflammatory effects for treating calvarial defects with graft application in rats 1 具有抗氧化和抗炎作用的灵芝在大鼠颅骨缺损移植中的应用前景

Acta Cirúrgica Brasileira Pub Date : 2019-11-25 DOI: 10.1590/s0102-865020190090000004

N. Laçin, Serhat Bozan İzol, Fikret İpek, M. Tuncer

{"title":"Ganoderma lucidum, a promising agent possessing antioxidant and anti-inflammatory effects for treating calvarial defects with graft application in rats 1","authors":"N. Laçin, Serhat Bozan İzol, Fikret İpek, M. Tuncer","doi":"10.1590/s0102-865020190090000004","DOIUrl":"https://doi.org/10.1590/s0102-865020190090000004","url":null,"abstract":"Abstract Purpose: Ganoderma lucidum, a kind of mushroom used for its antioxidant, anti-inflammatory, and immunomodulatory activities, was investigated in the present study for its possible healing effect on calvarial defects with bone grafts. Methods: Wistar male rats (n = 30) were divided into 3 groups: 1) the control (defect) group (n = 10), 2) defect and graft group (n = 10), and 3) defect, graft, and G. lucidum treated group (n = 10). The G. lucidum was administered to the rats at 20 mL/kg per day via gastric lavage. Results: In the defect and graft group, osteonectin positive expression was observed in osteoblast and osteocyte cells at the periphery of the small bone trabeculae within the graft area. In the defect, graft, and G. lucidum treated group, osteonectin expression was positive in the osteoblast and osteocyte cells and positive osteonectin expression in new bone trabeculae. The expression of matrix metalloproteinase-9 (MMP-9) was positive in the inflammatory cells, fibroblast cells, and degenerated collagen fibril areas within the defect area. Conclusion: This study shows that, with its antioxidant and anti-inflammatory properties, G. Lucidum is an important factor in the treatment of calvarial bone defects.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"199 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91452401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Stereological analysis of elastic fibers of the corpus cavernosum of rats during the aging process 1 衰老过程中大鼠海绵体弹性纤维的体视学分析

Acta Cirúrgica Brasileira Pub Date : 2019-10-14 DOI: 10.1590/s0102-865020190080000003

Thiago Hota, F. Lorenzini, Eduardo Felippe Melchioretto, M. Zeni, D. Veronez, R. D. Fraga

{"title":"Stereological analysis of elastic fibers of the corpus cavernosum of rats during the aging process 1","authors":"Thiago Hota, F. Lorenzini, Eduardo Felippe Melchioretto, M. Zeni, D. Veronez, R. D. Fraga","doi":"10.1590/s0102-865020190080000003","DOIUrl":"https://doi.org/10.1590/s0102-865020190080000003","url":null,"abstract":"Abstract Purpose To evaluate changes in the quantity of elastic fibers in the corpora cavernosa of rats during the natural aging process, and to assess the degree of this change by determining volumetric density (Vv) at different ages via stereological analysis. Methods Forty-eight rats, raised under similar conditions, were subjected to the natural aging process and divided into four groups (G1 to G4), according to age at the time of penectomy (6, 9, 12, and 24 months, respectively). Histological sections of the middle segment of the penis were stained with Weigert’s resorcin-fuchsin, and the volumetric density (Vv) of elastic fibers of the corpora cavernosa were determined via stereological analysis. Results There were no statistically significant differences in Vv among groups G1, G2, and G3. These three groups were therefore considered as a single group. The mean Vv of this group showed a statistically significant reduction compared to that of G4 (0.16 vs. 0.11, p<0.05). Conclusion Natural aging in rats was responsible for a reduction in volumetric density of elastic fibers of the corpora cavernosa (approximately 30% decrease in Vv) during senescence.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82032250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The effect of fibrin glue in preventing staple-line leak after sleeve gastrectomy. An experimental study in rats 1 纤维蛋白胶在预防袖式胃切除术后钉线泄漏中的作用。大鼠实验研究

Acta Cirúrgica Brasileira Pub Date : 2019-10-14 DOI: 10.1590/s0102-865020190080000001

Y. Ozdenkaya, O. Olmuşçelik, P. Basım, B. Saka, N. C. Arslan

{"title":"The effect of fibrin glue in preventing staple-line leak after sleeve gastrectomy. An experimental study in rats 1","authors":"Y. Ozdenkaya, O. Olmuşçelik, P. Basım, B. Saka, N. C. Arslan","doi":"10.1590/s0102-865020190080000001","DOIUrl":"https://doi.org/10.1590/s0102-865020190080000001","url":null,"abstract":"Abstract Purpose To evaluate the effect of fibrin glue on staple-line leak after sleeve gastrectomy. Methods Fourteen adult wistar rats 300 gr were randomized into two groups: Control group (n=7) and study group (n=7). All the rats underwent sleeve gastrectomy using lineer stapler. In the study group, fibrin glue was used to reinforce the staple-line. The rats were sacrificed 7 days after surgery. The stomach was resected, submerged in saline and exposed to excess pressure to obtain a burst pressure value. The gastric staple line was evaluated histopathologically according to the Ehrlich Hunt scale. The results of the two groups were compared. Results The mean Ehrlich-Hunt scores for inflammation, fibroblastic activity and neo-angiogenesis were similar between the groups (p>0.05). Collagen deposition was significantly higher in study group (3.42±0.53) when compared with control group (2.57±0.78) (p=0.035). The mean burst pressure was 137.8±8.5 mmHg for control group and 135.0±8.1 mmHg for study group (p=0.536). Conclusion Reinforcement of the staple-line with fibrin glue has no effect on the burst pressure after sleeve gastrectomy. More studies are needed to evaluate the precautions against leak after sleeve gastrectomy.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74114344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Effects of cyclosporine on ischemia-reperfusion injuries in rat kidneys. An experimental model 1 环孢素对大鼠肾缺血再灌注损伤的影响。实验模型1

Acta Cirúrgica Brasileira Pub Date : 2019-10-14 DOI: 10.1590/s0102-865020190080000006

Antonio Carlos Cerqueira Oliveira, N. S. Módolo, M. Domingues, P. Schwingel

{"title":"Effects of cyclosporine on ischemia-reperfusion injuries in rat kidneys. An experimental model 1","authors":"Antonio Carlos Cerqueira Oliveira, N. S. Módolo, M. Domingues, P. Schwingel","doi":"10.1590/s0102-865020190080000006","DOIUrl":"https://doi.org/10.1590/s0102-865020190080000006","url":null,"abstract":"Abstract Purpose To assess Cyclosporine A (CsA) therapy at an intraperitoneal dose of 15 mg.kg -1 in a rodent model of non-septic renal ischemia. Methods Twenty male Wistar rats were randomized to receive CsA therapy or none therapy before undergoing 30 minutes of renal ischemia followed by reperfusion. Additionally, 10 rats were randomized to undergo the same surgical procedure of the aforementioned animals with neither ischemia nor CsA therapy. Twelve hours after kidney ischemia, the left kidneys were evaluated for histological injury according to Park’s criteria. Serum creatinine (Cr), urea nitrogen (Ur) and sodium levels were obtained at different times of the experimental protocol. Results Rodents in the CsA group showed negative results (p<0.05) in serum variables (Cr: 0.41±0.05mg/dL vs . 4.17±1.25mg/dL; Ur: 40.90±3.98mg/dL vs . 187.70±22.93mg/dL) even the non CsA or control group (Cr: 0.35±0.07mg/dL vs . 3.80±1.20mg/dL; Ur: 40.10±4.70mg/dL vs . 184.50±49.80mg/dL). The negative results were also verified in histological evaluation, CsA group had 50% in the very severe grade of lesion, 10% in the severe and 40% in the moderate to severe whereas the control group had 90% in the very severe grade. Conclusion CsA was incapable of preventing the deleterious effects of ischemia-reperfusion injury in rat kidneys.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85824595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The effect of different flushing methods in a short peripheral catheter 1 不同冲洗方法对短外周导管的影响

Acta Cirúrgica Brasileira Pub Date : 2019-10-14 DOI: 10.1590/s0102-865020190080000004

Cuiling Tong, X. Peng, Hong Hu, Zongwen Wang, Hong Zhou

{"title":"The effect of different flushing methods in a short peripheral catheter 1","authors":"Cuiling Tong, X. Peng, Hong Hu, Zongwen Wang, Hong Zhou","doi":"10.1590/s0102-865020190080000004","DOIUrl":"https://doi.org/10.1590/s0102-865020190080000004","url":null,"abstract":"Abstract Purpose To develop a rabbit model of a short peripheral catheter (SPC) and to observe the effects of different flushing methods on blood vessels. Methods Thirty rabbits were randomly divided into three groups (A, B, and C), with ten rabbits per group. In group A, we used pulsed flush; in group B, we used uniform flush; and no treatment was used in group C. Results We observed that a uniform flush reduced blockage, phlebitis, and exudation compared to a pulsed flush by visual observation. The histopathological examination found that the morphological changes in group A were more severe than in group B and C related to loss of venous endothelial cells, inflammatory cell infiltration, edema, epidermal and chondrocyte degeneration, except for the thrombosis on group B that was more serious than in group A, especially in the distal side of puncture points. The distal region of groups A and B had more inflammatory cell infiltration than the proximal region. Thrombosis was more severe in the distal region than in the proximal region in group B. Conclusions The uniform flush produced less damage to the vascular endothelium and surrounding tissues and was superior to the pulsed flush. However, the uniform flush is prone to thrombosis.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85194573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6