Acta Cirúrgica Brasileira最新文献_第2页

Effects of curcumin supplementation on abdominal surgical wound healing 姜黄素补充剂对腹部手术伤口愈合的影响

Acta Cirúrgica Brasileira Pub Date : 2024-04-15 DOI: 10.1590/acb392124

Melquesedeque Dos Santos, Eurico Cleto Ribeiro de Campos, Rivair Gonçalves Junior, A. Koga, Pedro Afonso Kono, Matheus Von Jelita Salina, Elder Dalazoana Filho, Alceu de Oliveira Toledo Júnior, Leandro Cavalcante Lipinski

{"title":"Effects of curcumin supplementation on abdominal surgical wound healing","authors":"Melquesedeque Dos Santos, Eurico Cleto Ribeiro de Campos, Rivair Gonçalves Junior, A. Koga, Pedro Afonso Kono, Matheus Von Jelita Salina, Elder Dalazoana Filho, Alceu de Oliveira Toledo Júnior, Leandro Cavalcante Lipinski","doi":"10.1590/acb392124","DOIUrl":"https://doi.org/10.1590/acb392124","url":null,"abstract":"ABSTRACT Purpose: To evaluate the effects of curcumin supplementation on abdominal surgical wound healing in rats using clinical, histological, and hematological parameters. Methods: Forty Wistar rats were randomly divided into two groups: the curcumin group, and the control group. The curcumin group received, in addition to water and standard feed, curcumin via gavage at the dose of 200 mg/kg for seven days preceding and seven days following surgery. The control group received only water and standard feed. Both groups underwent median laparotomy and left colotomy. On the eighth postoperative day, the groups were euthanized, and the left colon was resected for histological analysis. Results: In the preoperative evaluation, there was a significant decrease in the mean C-reactive protein levels in the curcumin group (0.06) compared to the control group (0.112) (p = 0.0001). In the postoperative wound healing assessment, a significant decrease was observed in inflammatory infiltrate (p = 0.0006) and blood vessel count (p = 0.0002) in the curcumin group compared to the control group. Conclusions: Curcumin supplementation was able to significantly reduce inflammatory parameters in both pre-and post-operative phases of abdominal surgical wounds in rats.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"20 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140702017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential influences of expression levels of MFGE8 and HMGB1 on the intestinal mucosal barrier function and inflammatory response after blunt abdominal injury in rats MFGE8和HMGB1表达水平对钝性腹部损伤大鼠肠黏膜屏障功能和炎症反应的潜在影响

Acta Cirúrgica Brasileira Pub Date : 2022-06-01 DOI: 10.1590/acb370303

Li-Ping Tao, Hongbo Xu, Qianggui He

{"title":"Potential influences of expression levels of MFGE8 and HMGB1 on the intestinal mucosal barrier function and inflammatory response after blunt abdominal injury in rats","authors":"Li-Ping Tao, Hongbo Xu, Qianggui He","doi":"10.1590/acb370303","DOIUrl":"https://doi.org/10.1590/acb370303","url":null,"abstract":"ABSTRACT Purpose: To explore the influence of milk fat globule-EGF factor 8 protein (MFGE8) on blunt abdominal injury in Sprague Dawley (SD) rats through the RhoA/ROCK signaling pathway. Methods: The blunt abdominal injury model was generated in SD rats. A total of 44 rats was randomly assigned into three groups. Rat blunt abdominal injury was assessed by the abbreviated injury scale (AIS). The rats were sacrificed for observing the morphology of the abdominal cavity and intestines. Hematoxylin and eosin staining was performed to visualize the pathological changes of rat intestines. Positive expressions of MFGE8 and high mobility group box 1 (HMGB1) in rat intestines were examined by immunohistochemical staining. Protein levels were determined by Western blot. Serum levels of tumor necrosis factor α (TNF-α), IL-1β, IL-6 and malondialdehyde (MDA) were measured by enzyme linked immunosorbent assay (ELISA). Results: Blunt abdominal injury resulted in inflammatory response of intestinal tissues, increased serum levels of TNF-α, IL-1β, IL-6 and MDA, upregulation of HMGB1, RhoA and ROCK2, and downregulation of MFGE8 in rats, which were significantly alleviated by intervention of rhMFGE8. Conclusions: MFGE8 protects the intestinal mucosal barrier function after blunt abdominal injury in rats by downregulating HMGB1. Moreover, it alleviates inflammatory response and oxidative stress caused by blunt abdominal injury in rats through downregulating RhoA and ROCK.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"277 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79598343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Protective effect and mechanism of Shenkang injection on adenine-induced chronic renal failure in rats 肾康注射液对腺嘌呤所致大鼠慢性肾功能衰竭的保护作用及机制

Acta Cirúrgica Brasileira Pub Date : 2022-06-01 DOI: 10.1590/acb370304

Rongchang Chen, Lijiao Xu, Xu Zhang, Guibo Sun, Wenying Zeng, Xiaobo Sun

{"title":"Protective effect and mechanism of Shenkang injection on adenine-induced chronic renal failure in rats","authors":"Rongchang Chen, Lijiao Xu, Xu Zhang, Guibo Sun, Wenying Zeng, Xiaobo Sun","doi":"10.1590/acb370304","DOIUrl":"https://doi.org/10.1590/acb370304","url":null,"abstract":"ABSTRACT Purpose: To investigate the protective effects of Shenkang injection (SKI) on adenine-induced chronic renal failure (CRF) in rat. Methods: Sprague Dawley rats were randomly divided into five groups: control, model, and SKI groups (5, 10, 20 mL/kg). Rats in model and SKI groups were treated with adenine i.g. at a dose of 150 mg/kg every day for 12 weeks to induce CRF. Twelve weeks later, SKI was administered to the rat i.p. for four weeks. The effects of SKI on kidney injury and fibrosis were detected. Results: SKI inhibited the elevation of the urine level of N-acetyl-b-D-glucosaminidase, kidney injury molecule-1, beta-2-microglobulin, urea protein in CRF rats. The serum levels of uric acid and serum creatinine increased and albumin decreased in the model group, which was prevented by SKI. SKI inhibited the release of inflammatory cytokines and increasing the activities of antioxidant enzymes in serum. SKI inhibited the expression of transforming growth factor-β1, vascular cell adhesion molecule 1, intercellular adhesion molecule 1, collagen I, collagen III, endothelin-1, laminin in kidney of CRF rats. Conclusions: SKI protected against adenine-induced kidney injury and fibrosis and exerted anti-inflammatory, and antioxidant effects in CRF rats.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"101 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91460019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Anatomical description of the ventral and dorsal cervical rootlets in rats: A microsurgical study 大鼠颈根腹侧和背侧的解剖描述:显微外科研究

Acta Cirúrgica Brasileira Pub Date : 2022-06-01 DOI: 10.1590/acb370307

D. Santos, Nayara Pontes de Araújo, R. Teixeira, L. Bentes, D. Giubilei, Rosa Helena de Figueiredo Chaves, A. Gonçalves, Edson Yuzur Yasojima, R. S. M. Barros

{"title":"Anatomical description of the ventral and dorsal cervical rootlets in rats: A microsurgical study","authors":"D. Santos, Nayara Pontes de Araújo, R. Teixeira, L. Bentes, D. Giubilei, Rosa Helena de Figueiredo Chaves, A. Gonçalves, Edson Yuzur Yasojima, R. S. M. Barros","doi":"10.1590/acb370307","DOIUrl":"https://doi.org/10.1590/acb370307","url":null,"abstract":"ABSTRACT Purpose: To describe the anatomical aspects of the cervical rootlets and to quantify the number of rootlets that compose C1 to T1. Methods: Twenty male rats were used in this study. The dorsal rootlets from C1 to T1 were analyzed. To study the ventral rootlets, the posterior root avulsion was performed using a microhook, allowing exposure of the ventral roots through manipulation of the denticulate ligament and arachnoid mater. The parameters analyzed were the number of ventral and dorsal rootlets by side and level. Results: The formation of the respective spinal nerve was observed in the spinal roots the union of the ventral and dorsal roots. In four animals the C1 spinal root had no dorsal and/or ventral contribution. There is no normal pattern of numerical normality of the dorsal and ventral rootlets. The average number of fascicles per root was 4.08, with a slight superiority on the left side. There was a slight superiority of the dorsal rootlets compared to the ventral rootlets. Conclusions: This investigation was the first to study cervical rootlets in rats. In 20% of the sample studied, the dorsal root of C1 was absent mainly on the left side. There is a nonlinear numerical increase from C1 to T1 in the rootlets. There is a numerical predominance of cervical fascicles on the left side, confronting several studies related to the functional predominance of right laterality, requiring new studies that correlate these variables.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74185498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

ERRATA: Evaluation of psychological symptoms in patients before and after simultaneous pancreas-kidney transplantation: a single-center cross-sectional study 评估同时胰肾移植前后患者的心理症状:一项单中心横断面研究

Acta Cirúrgica Brasileira Pub Date : 2022-05-23 DOI: 10.1590/acb370202.errata

引用次数: 0

Ulinastatin alleviates early brain injury after intracerebral hemorrhage by inhibiting necroptosis and neuroinflammation via MAPK/NF-κB signaling pathway 乌司他丁通过MAPK/NF-κB信号通路抑制坏死下垂和神经炎症，减轻脑出血后早期脑损伤

Acta Cirúrgica Brasileira Pub Date : 2022-05-13 DOI: 10.1590/acb370301

Li Wang, Wei Jiao, Jiayu Wu, Jing Zhang, Min Tang, Yanghong Chen

{"title":"Ulinastatin alleviates early brain injury after intracerebral hemorrhage by inhibiting necroptosis and neuroinflammation via MAPK/NF-κB signaling pathway","authors":"Li Wang, Wei Jiao, Jiayu Wu, Jing Zhang, Min Tang, Yanghong Chen","doi":"10.1590/acb370301","DOIUrl":"https://doi.org/10.1590/acb370301","url":null,"abstract":"ABSTRACT Purpose: Spontaneous intracerebral hemorrhage (ICH) is a major public health problem with a huge economic burden worldwide. Ulinastatin (UTI), a serine protease inhibitor, has been reported to be anti-inflammatory, immune regulation, and organ protection by reducing reactive oxygen species production, and inflammation. Necroptosis is a programmed cell death mechanism that plays a vital role in neuronal cell death after ICH. However, the neuroprotection of UTI in ICH has not been confirmed, and the potential mechanism is unclear. The present study aimed to investigate the neuroprotection and potential molecular mechanisms of UTI in ICH-induced EBI in a C57BL/6 mouse model. Methods: The neurological score, brain water content, neuroinflammatory cytokine levels, and neuronal damage were evaluated. The anti-inflammation effectiveness of UTI in ICH patients also was evaluated. Results: UTI treatment markedly increased the neurological score, alleviate the brain edema, decreased the inflammatory cytokine TNF-α, interleukin‑1β (IL‑1β), IL‑6, NF‑κB levels, and RIP1/RIP3, which indicated that UTI-mediated inhibition of neuroinflammation, and necroptosis alleviated neuronal damage after ICH. UTI also can decrease the inflammatory cytokine of ICH patients. The neuroprotective capacity of UTI is partly dependent on the MAPK/NF-κB signaling pathway. Conclusions: UTI improves neurological outcomes in mice and reduces neuronal death by protecting against neural neuroinflammation, and necroptosis.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82605348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Development and characterization of poultry collagen-based hybrid hydrogels for bone regeneration 家禽骨再生胶原基混合水凝胶的研制与表征

Acta Cirúrgica Brasileira Pub Date : 2022-05-13 DOI: 10.1590/acb370302

Francisco Fábio Pereira de Souza, J. A. Pérez-Guerrero, M. J. Gomes, F. L. Cavalcante, M. D. S. S. Souza Filho, Igor Iuco Castro‐Silva

{"title":"Development and characterization of poultry collagen-based hybrid hydrogels for bone regeneration","authors":"Francisco Fábio Pereira de Souza, J. A. Pérez-Guerrero, M. J. Gomes, F. L. Cavalcante, M. D. S. S. Souza Filho, Igor Iuco Castro‐Silva","doi":"10.1590/acb370302","DOIUrl":"https://doi.org/10.1590/acb370302","url":null,"abstract":"ABSTRACT Purpose: Poultry by-products can contribute as an innovative natural source for the development of composites based on polymers and minerals aiming at bone regeneration. The objective of this study was the physicochemical and biological characterization of collagen-based hydrogels crosslinked with ultraviolet (UV)-riboflavin. Methods: Pure hydrogels of 100% collagen (G1) or hybrid hydrogels, 90% collagen:10% apatite (G2), 90% collagen:10% nanokeratin (G3), and 90% collagen:5% apatite:5% nanokeratin (G4) were characterized by scanning electron microscope, Fourier-transform infrared spectroscopy, differential scanning calorimetry, swelling degree and quali-quantitative histological analysis. Ectopic implantation in subcutaneous tissue in mice at one, three and nine weeks allowed to assess the inflammation (neutrophils, lymphocytes, macrophages, and giant cells) and repair (neovascularization, and connective tissue) to determine biocompatibility and the integrity of biomaterials to score their biodegradability. Histomorphometry on critical size defects in rat calvaria at one and three months evaluated the percentage of bone, connective tissue, and biomaterials in all groups. Results: The hydrogels presented porous microstructure, water absorption and physicochemical characteristics compatible with their polymeric and/or mineral composition. All materials exhibited biocompatibility, biodegradability, and low osteoconductivity. G2 showed greater density of new bone and biomaterial than the G1, G3 and G4. Conclusions: The collagen-apatite group formulation suggests potential for development as osteopromoting membrane.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84686301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Immunomodulatory effects of Blaps rynchopetera extract 龙舌兰提取物的免疫调节作用

Acta Cirúrgica Brasileira Pub Date : 2022-05-02 DOI: 10.1590/acb370205

Di Meng, Yinhe Yang, Longxing Li, Xiaoli Qian, Qi Wang, Jinglei Xu, Hairong Zhao, Heng Liu, Huai Xiao, Zhongtao Ding

引用次数: 1

Anatomorphometry of the brachial plexus under high-definition system: an experimental study in rats 高清系统下大鼠臂丛解剖形态学的实验研究

Acta Cirúrgica Brasileira Pub Date : 2022-05-02 DOI: 10.1590/acb370206

R. S. M. Barros, D. Santos, R. Teixeira, Nayara Pontes de Araújo, Danusa Neves Somensi, Atylla de Andrade Candido

{"title":"Anatomorphometry of the brachial plexus under high-definition system: an experimental study in rats","authors":"R. S. M. Barros, D. Santos, R. Teixeira, Nayara Pontes de Araújo, Danusa Neves Somensi, Atylla de Andrade Candido","doi":"10.1590/acb370206","DOIUrl":"https://doi.org/10.1590/acb370206","url":null,"abstract":"ABSTRACT Purpose: To study the anatomorphometry of the plexus brachialis (PB) of rats under a high-definition video system. Methods: Ten male Wistar rats discarded from other research that did not interfere in the morphology of the animal, respecting the principle of reduction, were used. All animals were submitted to the same protocol. Initially, the cervical region was shaved. The animals were placed in a dorsal position. A single elbow-to-elbow incision was performed and dissection started at the deltopectoral sulcus. The procedures were performed under a video system. To measure the structures, the Image J software was used. Results: All the PB evaluated originated from the C5-T1 spinal nerves. C5 and C6 converged to form the truncus superior, the root of C7 originated the truncus medius, and the confluence of C8 and T1 originated the truncus inferior. It was found the union of C7, C8, and T1 to form truncus inferomedialis instead of separate medial and inferior truncus. C8 (1.31 mm) was the thickest root, the truncus inferior (1.80 mm) and the nerve radialis (1.02 mm), were the thickest. Conclusions: The anatomy of the PB is comparable to humans, admitting variations. The videomagnification system is useful to perform microsurgical dissection.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"203 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74783613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Mechanism of dexmedetomidine preconditioning on spinal cord analgesia in rats with functional chronic visceral pain 右美托咪定预处理对功能性慢性内脏痛大鼠脊髓镇痛的作用机制

Acta Cirúrgica Brasileira Pub Date : 2022-05-02 DOI: 10.1590/acb370203

Jun Li, H. Tang, W. Tu

{"title":"Mechanism of dexmedetomidine preconditioning on spinal cord analgesia in rats with functional chronic visceral pain","authors":"Jun Li, H. Tang, W. Tu","doi":"10.1590/acb370203","DOIUrl":"https://doi.org/10.1590/acb370203","url":null,"abstract":"ABSTRACT Purpose: To analyze the effect and mechanism of dexmedetomidine (DEX) analgesia pretreatment on functional chronic visceral pain in rats. Methods: Rats were divided into six groups: W1, W2, W3, W4, W5, and W6. The behavioral changes and electrophysiological indexes of rats in each group before and after DEX treatment were detected. Results: The levels of abdominal withdrawal reflex (AWR) in W5 and W6 groups were significantly lower than those in group W3, while the levels of thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) were significantly higher than those in group W3 (p < 0.05). The electromyographic signals of W1, W5, and W6 groups showed little fluctuation, while those of groups W2, W3, and W4 showed obvious fluctuation. TLR4 mRNA expression, IRF3, P65, and phosphorylation levels in W4, W5, and W6 groups were significantly lower than those in group W2 (p < 0.05). Conclusions: Dexmedetomidine epidural anesthesia pretreatment could significantly inhibit visceral pain response in rats with functional chronic visceral pain, and its mechanism was related to the activation of TLR4 in spinal dorsal horn tissue of rats and the activation inhibition of IRF3 and P65 in the downstream key signals.","PeriodicalId":6993,"journal":{"name":"Acta Cirúrgica Brasileira","volume":"447 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78572618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1