{"title":"Determination of salt tolerance\u0000levels and genetic relationships of Vicia sativa cultivars using gene targeted functional\u0000markers","authors":"I. Tiryaki, Nuray Isidogru","doi":"10.37427/botcro-2022-005","DOIUrl":"https://doi.org/10.37427/botcro-2022-005","url":null,"abstract":"The objectives of the present study were to determine salt tolerance\u0000levels of 12 different common vetch (Vicia sativa L.) cultivars at\u0000germination stage in the presence of 250 mM NaCl and to reveal genetic\u0000relationships based on gene targeted functional markers (GTFMs) associated with\u0000salt tolerance. The results revealed the presence of a significant genetic\u0000variation among the cultivars although s alt stress significantly reduced all germination\u0000parameters tested. The cultivar Ozveren was the most salt tolerant with 20.1%\u0000reduction in final germination percentage compared to control seeds while\u0000cultivars Alınoglu, Ayaz and Bakir did not germinate. The maximum delays in germination rate (G50 =\u00003.78 days) and synchrony (G10-90 =\u00003.45 days) were obtained from the cultivars Urkmez and Ozveren, respectively. The GTFMs\u0000provided a total of 53.1% polymorphism. The primers of MtSOS2\u0000gene gave the highest numbers of alleles per primer pair while the highest\u0000polymorphism rate (77.8%) was obtained from the MtP5CS gene. The\u0000first three components of principal component analysis explained 57.63% of\u0000total variation. This study concluded that the cultivars determined to be salt\u0000tolerant and sensitive at germination stage distributed into three main clades\u0000determined by UPGMA analysis while the GTFMs associated with salt\u0000tolerance successfully\u0000determined the genetic relationships of common vetch cultivars.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43697287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}