水生生物学报最新文献

{"title":"ASSESSMENT OF PREDATION RISKS OF SEVEN PREDACIOUS FISH ON JUVENILE CHINESE MITTEN CRAB (ERIOCHEIR SINENSIS): ASSESSMENT OF PREDATION RISKS OF SEVEN PREDACIOUS FISH ON JUVENILE CHINESE MITTEN CRAB (ERIOCHEIR SINENSIS)","authors":"Mingzhong Luo, Tanglin Zhang, Chaowen Zhang, Zhongjie Li, Jiashou Liu, Xiao-Ping Wu","doi":"10.3724/SP.J.1035.2012.00932","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2012.00932","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":"36 1","pages":"932-938"},"PeriodicalIF":0.0,"publicationDate":"2013-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70060004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different nitrogen sources on growth and lipid accumulation of a heterotrophic microalgae-Chorella vulgaris.","authors":"Zhu Yiping, Song Donghui, Yang Guolan","doi":"10.3724/SP.J.1035.2012.01027","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2012.01027","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":"36 1","pages":"1027-1034"},"PeriodicalIF":0.0,"publicationDate":"2013-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70060245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity variation and community succession of aquatic macrophytes in Lake Futou.","authors":"Li Zhongqiang, Ren Hui, Hao Mengxi, Zhang Meng, Xu Jun","doi":"10.3724/SP.J.1035.2012.01018","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2012.01018","url":null,"abstract":"Due to extensive human activities,habitat loss and segmentation induce destruction and disappearance of aquatic plants in the shallow lakes.These activities cause secondary extinctions of aquatic animals,leading to loss of biodiversity in lakes and animal reproduction.Lake Futou,locates in southeastern Hubei Province,China,has the same functions,such as irrigation and aquaculture,as other shallow lakes in the middle and lower reaches of the Yangtze River.However,in recent 50 years,human disturbances,including reclamation,damming and over-fishing,have caused accelerated loss of diversity of aquatic plants.This worsened the ecosystem fuction and seriously affected the productivity of aquatic resources and the quality of species.Thus,the aims of the this study were to understand the succession of aquatic plants in Lake Futou in recent decades and to comprehend the reasons associated with exploitation and utilization of resources in this lake.In order to investigate the aquatic plant diversity in Lake Futou,the species and community diversity were studied using field investigation in 2009,and the topology were analysed by geographical information system technique with ARC/INFO.Based on the results obtained from field investigation and previous studies,species diversity variations and succession of aquatic macrophytes community in Lake Futou from 1988 to 2009 were investigated and the reasons between the changes and some controlling factors were analyzed.The results showed that there were some variations on the number of species and dominant species of aquatic plants.Some species that were sensitive to disturbances such as Nymphaea stellata disappeared and some dominant species such as Potamogeton maackianus,Vallisneria denseserrulata and Hydrilla verticillata became sub-dominant species or accompanying species from 1988 to 2009.In addition,the introduced species such as Elodea nuttalli occurred in Lake Futou and became dominant species.Before the early 1980s,vegetation coverage was 100%.At the end of 1980s,coverage decresed to 80.5%,and the coverage further decresed significantly to 16.7% in 1999 and increased to 47.9% in 2009.From 1988 to 2009,emergent macrophytes increased the coverage from 24.18% to 56.93%,and Nelumbo nucifera became the dominant species in the summer.In this period,the coverages of submersed and floating-leaved macrophytes declined from 47.25% and 28.57% to 29.21% and 10.35%,respectively.Associations dominated by Nymphoides peltata in floating-leaved plants and dominated by Elodea nuttalli in submersed plants increased in the communities.Associations of Ceratophyllum demersum,Myriophyllum spicatum,and Ceratophyllum demersum also increased.Potamogeton maackianus decreased significantily.Overall,the aquatic macrophyte communities in the lake have changed markedly since the natural succession status in the 1950s changed toward the artificial succession status due to human disturbances observed in this study.Vegetation type,vegetatio","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":"36 1","pages":"1018-1026"},"PeriodicalIF":0.0,"publicationDate":"2013-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70060164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANALYSIS OF GENETIC DIVERSITY OF FOUR TAKIFUGU OBSCURUS POPULATIONS USING SRAP MARKERS","authors":"Chen Changhong, Zhang Minying, Liu Kai, Xu Dongpo, Duan Jinrong, Zhou Yanfeng, Shi Weigang","doi":"10.3724/SP.J.1035.2012.00858","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2012.00858","url":null,"abstract":"Takifugu species(order: Tetradotiformes;family: Tetradontidae) have attracted attention among the scientific community for their peculiar biology,physiology and genomics.Obscure puffer(Takifugu obscurus) is an anadromous fish species in China.They usually grow in the sea before turning sexually mature,then migrate to freshwater rivers for reproduction during the spawning season.Obscure puffer is a popular fish in China for its high-quality meat.In 2002,annual production of obscure puffer was 2600 tons,and it has been continuing to rise owing to its large body size,rapid growth,and high market value of the fish.However,obscure puffer resource has sharply declined because of overfishing and water pollution.Previous studies have focused on the physiology and mitochondrial DNA.Analysis of the genetic diversity and structure of a fish species is an important prerequisite for conservation as it reflects the status and survival potential of this species.However,the genetic diversity among and within populations of obscure puffer populations remains unknown,thus it is difficult to implement effective conservation strategies.Molecular markers are useful for population genetic studies to assess the influence of various factors on genetic diversity and population structure.Among the many types of molecular markers,sequence-related amplified polymorphism(SRAP) has been recognized as a new and useful molecular marker system because of its simplicity,reproducibility and convenience.This method is based on two-primer amplification.The primers are 17 or 18 nucleotides long and consist of the following elements.SRAP amplifies open reading frames and is significant for gene and genetic diversity.It has been applied extensively in molecular identification,genetic linkage map construction,gene tagging,genetic diversity analysis and comparative genetics of different species.In this study,in order to investigate the genetic diversity and population structure among and within populations of obscure puffer,a SRAP marker was firstly used to analyze the genetic diversity of four obscure puffer populations.These populations included a captured population in the Yangtze Rive,a released population and two aquaculture populations.18 pairs of primer combinations,which were selected from 49 primer pairs,were applied to four populations.A total of 231 loci were detected,among which 156 were polymorphic.The proportion of polymorphic loci ranged from 54.98% to 58.87% in these four populations.The Nei’s genetic diversity(H) of the four populations were between 0.1992 and 0.2005,while the Shannon information index(I) were between 0.2953 and 0.3016.The gene flow(Nm) was 4.1291 among populations,of which,the proportion of polymorphic loci,the Nei’s genetic diversity(H) and Shannon information index(I) of the captured population were a little higher than the other three populations.Individual clustering showed that the Shanghai population formed an independent cluster and the other thr","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":"36 1","pages":"858-864"},"PeriodicalIF":0.0,"publicationDate":"2013-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70059654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GENETIC STRUCTURE AND DIVERSITY OF SCHIZOTHORAX BIDDULPHI POPULATIONS: GENETIC STRUCTURE AND DIVERSITY OF SCHIZOTHORAX BIDDULPHI POPULATIONS","authors":"Wei Meng, Yan Guo, Sa Hai, Tianyan Yang, Yan-wu Ma, Chun-gang Xie","doi":"10.3724/SP.J.1035.2012.00851","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2012.00851","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":"36 1","pages":"851-857"},"PeriodicalIF":0.0,"publicationDate":"2013-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70059565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GROWTH AND AFLATOXIN B1 ACCUMULATION OF GIBEL CARP ADULT FED WITH DIETS OF DIFFERENT LEVELS OF AFLATOXIN B1: GROWTH AND AFLATOXIN B1 ACCUMULATION OF GIBEL CARP ADULT FED WITH DIETS OF DIFFERENT LEVELS OF AFLATOXIN B1","authors":"Ying Huang, Xiaoming Zhu, Dong Han, Yunxia Yang, Junyan Jin, Haiyang Li, Yifeng Chen, S. Xie","doi":"10.3724/SP.J.1035.2012.00817","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2012.00817","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":"36 1","pages":"817-825"},"PeriodicalIF":0.0,"publicationDate":"2013-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70059727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECTS OF DIETARY PROTEIN AND LIPID LEVELS ON GROWTH, FEED UTILIZATION AND BODY COMPOSITION OF CYPRINUS LONGIPECTORALIS: EFFECTS OF DIETARY PROTEIN AND LIPID LEVELS ON GROWTH, FEED UTILIZATION AND BODY COMPOSITION OF CYPRINUS LONGIPECTORALIS","authors":"Yongqin Tu, Dong Han, Xiaoming Zhu, Yunxia Yang, S. Xie","doi":"10.3724/SP.J.1035.2012.00843","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2012.00843","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":"36 1","pages":"843-850"},"PeriodicalIF":0.0,"publicationDate":"2013-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70059969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CLONING AND CHARACTERIZATION OF DRSPIN-1 GENE IN ZEBRAFISH (DANIO RERIO)","authors":"W. Xiao","doi":"10.3724/sp.j.1035.2012.00874","DOIUrl":"https://doi.org/10.3724/sp.j.1035.2012.00874","url":null,"abstract":"Spin protein family,with the conserved Spin/Ssty motif,play important role during gametogenesis and early embryo development.So far,no Spin genes have been identified in zebrafish,Danio rerio.In this study,to isolate the full-length cDNA of Spin gene from zebrafish,we designed two degenerate primers according to the conserved sequences of Spin protein family.Two 260 bp DNA fragments of DrSpin-1 and DrSpin-2 were isolated from SMART cDNA library of zebrafish mature eggs.The sequence homologous analysis indicated the identity of amino acid sequences between DrSpin-1 protein and CagSpin protein got up to 81%,and DrSpin-2 protein showed greater sequence identity with OlSpinB and OlSpinC.Further,two specific primers were designed according to the 260 bp DNA sequence of DrSpin-1 for RACE polymerase chain reaction,and the full-length cDNA sequences of DrSpin-1 was isolated from the SMART cDNA library referred above.Sequence analysis revealed DrSpin-1 cDNA contained 1082 base pairs with a 771 base pairs open reading frame encoding 257 amino acids and including three conserved Spin/Ssty motives and eight possible phosphorylation sites.These data confirmed the cloned DrSpin-1 gene was one of Spin gene family.Furthermore,the multiple alignments analysis of DrSpin-1 protein sequence and Spin protein sequences in other fishes by CLUSTRA W indicated that DrSpin-1 protein had the highest homology to CagSpin protein in gibel carp.Therefore,we suggested DrSpin-1 in zebrafish might have the similar expression pattern to CagSpin,and might play important roles in regulation during oogenesis and fertilization.Our studies provide the clue to further functional research of Spin in zefrafish.","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70059696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECT OF ACUTE SALINITY STRESS ON SOLUBLE PROTEIN,HEMOCYANIN,HAEMOLYMPH GLUCOSE AND HEPATOPANCREAS GLYCOGEN OF ERIOCHEIR SINENSIS","authors":"W. Yue","doi":"10.3724/sp.j.1035.2012.01056","DOIUrl":"https://doi.org/10.3724/sp.j.1035.2012.01056","url":null,"abstract":"Most of the decapod species of crustacean have comprehensive capacity of osmoregulation,and they can adapt to relative wide ambient salinity range.It is important to understand the underlying mechanisms of their comprehensive adaptability to salinity change because this knowledge is helpful for inland culturing of marine species.As a euryhaline crustacean,the Chinese mitten crab,Eriocheir sinensis,has been well studied in many aspects related to their physiological responses to salinity change,and many useful literature findings have been accumulated.Therefore,E.sinensis can be considered as an animal model for studying osmoregulation mechanism of the decapod species.However,the roles of nutrients in osmoregulation of E.sinensis are still limited.In this paper,male E.sinensis in freshwater(0.3‰) were transferred directly into waters at salinity values of 16‰ and 30‰,respectively,and the crabs kept at fresh water served as the control.Each treatment has three replicates.The soluble protein in haemolymph and hepatopancreas,hemocyanin,haemolymph glucose and hepatopancreas glycogen at 0h,6h,12h,24h,48h,72h and 96h of E.sinensis at different salinities were determined.Soluble protein of haemolymph and hepatopancreas were measured with a Folin reagent based on a standard curve using bovine serum albumin as the standard protein.Hemocyanin was assayed using an ultraviolet absorption methods at 335 nm and calculated according to Nickerson 1% 1cm E =2.83.Haemolymph glucose was assayed using glucose oxidase-peroxide enzyme(GOD-POD) method.Hepatopancreas glycogen was measured using the anthrone colorimetry method.The results showed that the crabs at salinity 16‰ and 30‰ had significantly lower soluble protein content of hepatopancreas than those at fresh water(P0.05) when crabs had encountered salt water for 12h—96h,and the soluble protein content of haemolymph decreased significantly from 6h to 48h,and then increased at 72h.The hemocyanin content showed a significantly decreasing trend from 0h to 24h,and then it increased after 48h.Hepatopancreas glycogen contents at salinity 16‰ and 30‰ were significantly lower than those at fresh water(P0.05) from 6h to 96h,while no significant differences were observed between crabs in waters with salinity 16‰ and 30‰(P0.05).Haemolymph glucose content at 16‰ reduced significantly(P0.05) from 24h to 48h,and gradually recovered up at 72h,but it showed a significantly decreasing trend from 6h to 12h at salinity 30‰,and then gradually increased after 24h.All these results indicated that E.sinensis could produce physiological and biochemical adaptation to maintain a stable osmotic pressure during acute salinity stress.Carbohydrate and protein play important roles in the osmoregulation of crustaceans.E.sinensis could use carbohydrate first to provide energy under acute salinity stress.The higher the salinity,the faster the haemolymph glucose was consumed,and it also recovered more quickly.This observation suggested that carboh","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70060387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECTS OF DIFFERENT PROTEIN AND LIPID LEVELS ON THE GROWTH PERFORMANCE AND BODY COMPOSITION OF BLUNT SNOUT BREAM (MEGALOBRAMA AMBLYCEPHALA) YEARLINGS","authors":"Jiang Yang","doi":"10.3724/sp.j.1035.2012.00826","DOIUrl":"https://doi.org/10.3724/sp.j.1035.2012.00826","url":null,"abstract":"A 3×3 factorial design was used to determine effect of dietary protein and lipid levels on growth performance and body composition of blunt snout bream yearlings(average initial weight of 50.37±1.27 g).Nine experimental diets were formulated to contain three protein levels(25%,30% and 35%),each of which had three lipid levels(3%,6% and 9%).Fish were held in floating cages(2 m×1 m×1 m) and fed three times daily for 8 weeks.No interaction between dietary protein and lipid existed for growth performance and body composition(P0.05).No significant difference(P0.05) was observed in survival rate among all the treatments.Weight gain(WG),specific growth rate(SGR) and feed conversion ratio(FCR) were significantly influenced by both dietary protein(P0.05) and lipid levels(P0.05).WG and SGR of fish fed with 25% protein were significantly(P0.05) lower than that of the other groups;whereas,the opposite was true for fish fed with 6% lipid(P0.01).Although the lowest FCR was found in fish fed with 35% protein and 6% lipid,no significant difference(P0.05) was observed among the other treatments except for that of fish fed with 25% protein + 3% lipid and 25% protein + 9% lipid.Protein efficiency ratio(PER) and nitrogen retention efficiency decreased significantly with increasing dietary protein levels(P0.05).In addition,PER was significantly(P0.05) affected by dietary lipid level with the highest observed in fish fed with 6% lipid.Energy retention efficiency increased significantly as dietary lipid levels increased(P0.05).Condition factor improved significantly(P0.05) as dietary protein and lipid levels both increased.Intraperitoneal fat ratio and hepatosomatic index increased significantly(P0.05) as dietary lipid levels increased.No significant difference(P0.05) was observed in body composition among all the treatments at different dietary protein levels.Moisture,lipid and energy contents of whole-body were significantly(P0.01) affected by dietary lipid levels.Contrary to moisture content,lipid and energy contents of whole-body in-creased significantly(P0.01) with increasing lipid level.Similar results were also observed in carcass and liver composition.The results of this study indicated that diet containing 30% protein and 6% lipid with a protein to energy ratio of 18.21 g/MJ was suitable for blunt snout bream yearlings.","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70059816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}