Acta endocrinologica. Supplementum最新文献_第5页

Effect of iodine intake and methimazole on lymphocytic thyroiditis in the BB/W rat. 碘摄入和甲巯咪唑对BB/W大鼠淋巴细胞性甲状腺炎的影响。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s070

L E Braverman, T Paul, W Reinhardt, M C Appel, E M Allen

{"title":"Effect of iodine intake and methimazole on lymphocytic thyroiditis in the BB/W rat.","authors":"L E Braverman, T Paul, W Reinhardt, M C Appel, E M Allen","doi":"10.1530/acta.0.114s070","DOIUrl":"https://doi.org/10.1530/acta.0.114s070","url":null,"abstract":"Spontaneous LT and elevated serum anti-Tg occur in the diabetes prone BB/W rat, but thyroid function is essentially normal in the rats with LT. Prolonged low dose MMI decreases the incidence of LT in BB/W rats. The administration of excess iodine beginning at 30 days of age markedly accelerates the occurrence of LT and anti-Tg at 90 days of age. Low iodine intake decreases the incidence of LT. Excess iodine intake did not induce LT in W-line, Wistar-Furth, and Sprague-Dawley rats. This suggests that iodine induced LT occurs only in genetically susceptible rats. Despite the increased incidence of LT during iodine administration, thyroid function remains essentially normal. This is in contrast to the frequent induction of hypothyroidism following iodine administration to euthyroid patients with Hashimoto's thyroiditis. In order to decrease thyroid reserve, rats were hemi-TX at 30 days of age. The administration of iodine markedly increased the incidence of LT and serum anti-Tg, increased the weight of the remaining lobe, and induced hypothyroidism as determined by significantly lower serum T4 and T3 concentrations and elevated serum TSH concentrations. Excess iodine administration to hemi-TX W-line rats (genetically equivalent, non-diabetes, non-LT prone BB/W rats) did not induce LT but did induce hypothyroidism, suggesting that BB/W and W-line rats are susceptible to iodine induced hypothyroidism, perhaps unrelated to the induction of LT. Excess iodine did not induce LT or affect thyroid function in hemi-TX Wistar-Furth and Sprague-Dawley rats.","PeriodicalId":6931,"journal":{"name":"Acta endocrinologica. Supplementum","volume":"281 ","pages":"70-6"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.114s070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14600526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Stimulation of Graves' thyroids in vitro. 格雷夫斯甲状腺的体外刺激。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01

R Hörmann, J Kirner, B Saller, K Mann

引用次数: 0

Thyrocyte HLA class II expression and regulation in relation to thyroid autoimmunity. 甲状腺细胞HLAⅱ类的表达与调节与甲状腺自身免疫的关系

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s027

I Todd, R Pujol-Borrell, A Belfiore, G F Bottazzo

{"title":"Thyrocyte HLA class II expression and regulation in relation to thyroid autoimmunity.","authors":"I Todd, R Pujol-Borrell, A Belfiore, G F Bottazzo","doi":"10.1530/acta.0.114s027","DOIUrl":"https://doi.org/10.1530/acta.0.114s027","url":null,"abstract":"The occurrence of HLA Class II expression by thyroid (and other endocrine) epithelia in autoimmune diseases suggests that these cells may facilitate their own destruction by immunogenically presenting autoantigens. This is supported by the findings that Class II+ thyrocytes can specifically stimulate virus-specific and autoreactive T cell clones, and that Class II expression by thyrocytes correlates with the occurrence of thyroid autoantibodies. A variety of factors may contribute to the regulation of Class II expression by thyrocytes: this is induced by interferon (IFN-gamma), and is enhanced by thyroid stimulating hormone (TSH) and by tumour necrosis factor (TNF). Conversely, epidermal growth factor (EGF) suppresses the induction of Class II in thyrocytes. This complex regulation is reflected in differences in HLA-D subregion expression between patients (DR greater than DP greater than DQ). The immune-based mechanisms of thyrocyte Class II regulation are clearly applicable to the on-going disease in an infiltrated thyroid, but the possibility of nonimmune Class II induction deserves attention, particularly in identifying factors which might contribute to the initial autoimmune attack. The possible involvement of such mechanisms in autoimmunity is supported by findings in Type I diabetes in which Class II+ islet beta cells can be found in the absence of infiltration. Further evidence is provided by the observation that a proportion of thyrocytes transformed with SV40 DNA constitutively express Class II molecules. Finally, the 'activated' state of capillary endothelial cells in organs subject to autoimmune attack suggests that they may play an important role in facilitating the autoreactive infiltration of the tissues.","PeriodicalId":6931,"journal":{"name":"Acta endocrinologica. Supplementum","volume":"281 ","pages":"27-34"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.114s027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14620412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Dysregulation of the immune system in obese strain chickens with Hashimoto-like thyroiditis: intrinsic and extrinsic mechanisms. 肥胖株鸡桥本样甲状腺炎的免疫系统失调:内在和外在机制。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01

K Schauenstein, R Fässler, G Krömer, G Wick

引用次数: 0

Restriction enzyme analysis of HLA class II DR beta genes in patients with Graves' disease. Graves病患者HLAⅱ类DR β基因的限制性内切酶分析。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s146

B O Boehm, E Schifferdecker, P Kuehnl, C Rosak, K Schöffling

引用次数: 1

Immunoreactivity of PTH-binding in intact bovine kidney tissue and cultured cortical kidney cells indicative for specific receptors. 完整牛肾组织和体外培养肾皮质细胞中甲状旁腺素结合的免疫反应性。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s207

A Niendorf, H Arps, M Sieck, M Dietel

{"title":"Immunoreactivity of PTH-binding in intact bovine kidney tissue and cultured cortical kidney cells indicative for specific receptors.","authors":"A Niendorf, H Arps, M Sieck, M Dietel","doi":"10.1530/acta.0.114s207","DOIUrl":"https://doi.org/10.1530/acta.0.114s207","url":null,"abstract":"Localization of PTH-binding sites has been examined in intact kidney sections and cultured cells derived from bovine kidney cortex. Tissue sections were incubated with 10(-7) M bovine PTH (1-84) for 2 h, cells for 15 min, at 37 degrees C. Visualization of PTH-binding was achieved by immunocytochemistry using a carboxy-terminal specific anti-PTH antiserum (S 478). For control, cell culture incubations were performed applying competitively 10(-7) bovine PTH (1-84) and a 10-fold excess of synthetic 1-34 PTH fragment, not antigenic for S 478. This resulted in a lack of staining. PTH-binding was found in all cells of the proximal and the distal tubule, and with less intensity in the thick ascending limb of Henle's loop. In collecting ducts a PTH specific staining was also present, which was confined to single cells localized between others without PTH binding sites. No staining was seen in glomerula, the thin limb of Henle's loop, in blood vessels, and in connective tissue. The data suggest that large parts of the nephron contain PTH-binding sites, although in different amounts. This is in agreement with the numerous actions of PTH in the kidney. In the collecting segment a distinct cell-to-cell difference was disclosed indicative for different functional states or cellular heterogeneity.","PeriodicalId":6931,"journal":{"name":"Acta endocrinologica. Supplementum","volume":"281 ","pages":"207-11"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.114s207","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14172288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Graves' IgG stimulates thyroid epithelial cell proliferation in xenotransplanted human toxic diffuse goitre. Graves IgG刺激异种移植人中毒性弥漫性甲状腺的甲状腺上皮细胞增殖。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s115

E Jörtsö, L Tegler, S Smeds

{"title":"Graves' IgG stimulates thyroid epithelial cell proliferation in xenotransplanted human toxic diffuse goitre.","authors":"E Jörtsö, L Tegler, S Smeds","doi":"10.1530/acta.0.114s115","DOIUrl":"https://doi.org/10.1530/acta.0.114s115","url":null,"abstract":"Human toxic diffuse goitre tissue was xenotransplanted to athymic mice. Transplant function was analyzed as 18 h [125I]thyroid transplant uptake at day 21 and at 10 weeks after transplantation. Graves' IgG or normal IgG was given ip daily day 22-35. Epithelial cell proliferation in the thyroid transplants was analyzed by continuous [3H]thymidine administration for 12 days between day 28 and 39 in a separate series given Graves' or normal IgG daily during the same period. The 18 h transplant uptake increased 12.8 times from 3 to 10 weeks in the Graves' IgG group but only 3.6 times in the controls (P less than 0.05). The fraction of labelled cells after [3H]thymidine incorporation was 51% +/- (SEM) after parallel Graves' IgG administration but only 2 +/- 0.3% (P less than 0.002) in the controls. The increased 10 weeks iodide uptake after Graves' IgG may be explained by an increased vascularisation or capillary maturation, by an increased individual cell sensitivity to stimulation or by an increased number of cells. Our results indicate that serum from patients with toxic diffuse goitre, i.e. Graves' IgG, contains a factor which promotes thyroid epithelial cell proliferation. Whether this is identical to TSI or is another IgG fraction remains to be shown.","PeriodicalId":6931,"journal":{"name":"Acta endocrinologica. Supplementum","volume":"281 ","pages":"115-7"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14598991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Dendritic cells in autoimmune thyroid disease. 自身免疫性甲状腺疾病中的树突状细胞。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s042

P J Kabel, H A Voorbij, R D van der Gaag, W M Wiersinga, M de Haan, H A Drexhage

{"title":"Dendritic cells in autoimmune thyroid disease.","authors":"P J Kabel, H A Voorbij, R D van der Gaag, W M Wiersinga, M de Haan, H A Drexhage","doi":"10.1530/acta.0.114s042","DOIUrl":"https://doi.org/10.1530/acta.0.114s042","url":null,"abstract":"Dendritic cells form a morphologically distinct class of cells characterized by shape, reniform nucleus, absent to weak acid-phosphatase activity and strong Class II MHC determinant positivity. Functionally they are the most efficient cells in antigen presentation to T-lymphocytes which indicates their role in the initiation of an immune response. Using immunehistochemical techniques we studied the presence of dendritic cells in normal Wistar rat and human thyroids, in thyroids of BBW rats developing thyroid autoimmunity and in Graves' goitres. Dendritic cells could be identified in all thyroids studied and were positioned underneath the thyrocytes in between the follicles. Skin dendritic cells travel via lymphatics to draining lymph nodes, thus forming an antigen presenting cell system. It is likely that a similar cell system exists on the level of the thyroid for dendritic cells have also been detected in thyroid draining lymph nodes. In normal thyroid tissue of both human and rat dendritic cells were relatively scarce. During the initial phases of the thyroid autoimmune response in the BBW rat (before the appearance of Tg-antibodies in the circulation) numbers of thyroid dendritic cells increased. Intrathyroidal T-helper cells, B-cells or plasma cells could not be found. The thyroid draining lymph node contained large numbers of plasma cells. During the later stages of the thyroid autoimmune response in the BB/W rat (after the appearance of Tg-antibodies in the circulation) and in Graves' goitres dendritic cells were not only present in high number, but 20-30% were seen in contact with now-present intrathyroidal T-helper lymphocytes.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":6931,"journal":{"name":"Acta endocrinologica. Supplementum","volume":"281 ","pages":"42-8"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.114s042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14600523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

TSH receptor structure. TSH受体结构。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01 DOI: 10.1530/acta.0.114s166

J Chan, P Santisteban, M De Luca, O Isozaki, E Grollman, L Kohn

{"title":"TSH receptor structure.","authors":"J Chan, P Santisteban, M De Luca, O Isozaki, E Grollman, L Kohn","doi":"10.1530/acta.0.114s166","DOIUrl":"https://doi.org/10.1530/acta.0.114s166","url":null,"abstract":"When solubilized, radiolabelled membrane preparations from FRTL-5 rat thyroid cells are applied to TSH affinity columns, two separate peaks of protein can be eluted by high salts/high pH and low pH buffers, respectively. Immunoprecipitation with monoclonal antibodies to the TSH receptor shows that both peaks contain proteins related to the TSH receptor. If extracts were from cells grown without TSH, one peak has a approximately 300 K and the other a approximately 70 K protein the 70 K protein can be derived from the purified 300 K protein in vitro. A 50 and 20 K protein can be derived from the 70 K protein. If extracts are from cells grown with TSH, the peaks contain a multiplicity of additional immuno-precipitable bands of approximately 200, 175, 130, 90, 50, 20 K etc. These bands are shown to result from the ability of TSH to increase the synthesis (3-4-fold) and degradation (2-3-fold) of the 300 and 70 K proteins. The 300/70 K protein fractions are reactive with monoclonal autoimmune thyroid stimulating antibodies and contain a specific disialo ganglioside. The ganglioside migrates near GM2, i.e., like a lower order ganglioside, and contains fucose. In translation experiments, the monoclonal antibodies to the TSH receptor identify a single mRNA component which produces a protein of approximately 220 K. This protein is not present in thyroid cells which have no functional TSH receptor and which cannot be surface labelled with monoclonal antibodies to the TSH receptor.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":6931,"journal":{"name":"Acta endocrinologica. Supplementum","volume":"281 ","pages":"166-72"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.114s166","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14600750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Paracrine interaction between thyrocytes and fibroblasts. 甲状腺细胞和成纤维细胞的旁分泌相互作用。

Acta endocrinologica. Supplementum Pub Date : 1987-01-01

R Gärtner, G Bechtner, D Stübner, W Greil

引用次数: 0