Acta endocrinologica最新文献_第9页

Brain area specific differences in the effects of neuroactive steroids on the GABAA receptor complexes following acute treatment with anaesthetically active steroids. 急性麻醉类固醇治疗后，神经活性类固醇对GABAA受体复合物影响的脑区特异性差异。

Acta endocrinologica Pub Date : 1993-11-01 DOI: 10.1530/acta.0.1290480

A Jussofie

{"title":"Brain area specific differences in the effects of neuroactive steroids on the GABAA receptor complexes following acute treatment with anaesthetically active steroids.","authors":"A Jussofie","doi":"10.1530/acta.0.1290480","DOIUrl":"https://doi.org/10.1530/acta.0.1290480","url":null,"abstract":"Regional differences in the effects of neuroactive steroids on the GABAA receptor complex have been demonstrated in vitro (1-3). In order to elucidate if a regionally different modulation of the GABA-binding site also occurs after acute steroidal treatment, in the present study the effects of systemically applied progesterone (10 mg/kg body wt) and its metabolite 3 alpha-hydroxy-5 alpha-pregnane-20-one (3 alpha, 5 alpha-THP) (2 mg/kg body wt) on the specific [3H]muscimol binding to the GABA-binding site was evaluated using membrane fractions prepared from five different brain areas of ovariectomized rats. The receptor density (mumol/kg protein) was not affected in all brain areas investigated by treatment with 3 alpha, 5 alpha-THP but was enhanced selectively in the frontal cortex and hippocampus of progesterone-treated animals. A decrease of affinity was observed in medulla after application of progesterone or 3 alpha, 5 alpha-THP, in contrast to the hypothalamus, which was the single region exhibiting an increase of affinity after steroidal administration. In the frontal cortex and hippocampus only the injection of progesterone reduced the affinity, whereas in cerebellum both steroidal treatments were incapable of affecting the affinity. These regional differences in response to the GABA-binding site to acutely applied progesterone and 3 alpha, 5 alpha-THP are consistent with the existence of heterogeneous populations of GABAA receptor-coupled steroid-binding sites.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 5","pages":"480-5"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290480","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19268488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Stimulatory effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on insulin and glucagon release from the isolated perfused rat pancreas. 垂体腺苷酸环化酶激活多肽(PACAP)对大鼠离体胰腺胰岛素和胰高血糖素释放的刺激作用。

Acta endocrinologica Pub Date : 1993-11-01 DOI: 10.1530/acta.0.1290473

C Yokota, K Kawai, S Ohashi, Y Watanabe, S Suzuki, K Yamashita

{"title":"Stimulatory effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on insulin and glucagon release from the isolated perfused rat pancreas.","authors":"C Yokota, K Kawai, S Ohashi, Y Watanabe, S Suzuki, K Yamashita","doi":"10.1530/acta.0.1290473","DOIUrl":"https://doi.org/10.1530/acta.0.1290473","url":null,"abstract":"Rat pancreas perfusion was performed to study the effects of pituitary adenylate cyclase activating polypeptide (PACAP) on pancreatic hormone release. Under the perfusate glucose concentration of 5.5 mmol/l, 0.1 nmol/l PACAP27 significantly stimulated both insulin and glucagon release. The degree of stimulation was in a dose dependent manner. The stimulation of insulin release was clearly dependent on the perfusate glucose concentration, when compared with 2.8, 5.5 and 8.3 mmol/l. The potency of PACAP38 on the stimulation of insulin release was greater than that of PACAP27 at 5.5 mmol/l of perfusate glucose concentration, but not at 8.3 mmol/l. No differences for glucagon and cAMP release were found between the two peptides. PACAP's stimulatory effects on insulin and glucagon release were completely abolished by an equimolar and ten times lower concentration of somatostatin, respectively. The physiologic significance of these potent effects of PACAP's islet hormones release must be clarified by further studies.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 5","pages":"473-9"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290473","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18902238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Isolation and partial molecular characterization of basic fibroblast growth factor from isolated porcine thyroid follicles and entire porcine thyroid glands. 猪甲状腺滤泡及全猪甲状腺碱性成纤维细胞生长因子的分离及部分分子特性研究。

Acta endocrinologica Pub Date : 1993-11-01 DOI: 10.1530/acta.0.1290458

G Bechtner, B Rieder, U Linsenmaier, J Kellermann, W Greil, R Gärtner

{"title":"Isolation and partial molecular characterization of basic fibroblast growth factor from isolated porcine thyroid follicles and entire porcine thyroid glands.","authors":"G Bechtner, B Rieder, U Linsenmaier, J Kellermann, W Greil, R Gärtner","doi":"10.1530/acta.0.1290458","DOIUrl":"https://doi.org/10.1530/acta.0.1290458","url":null,"abstract":"Immunoreactive basic fibroblast growth factor (bFGF) could be isolated from the cytosol preparation of isolated porcine thyroid follicles as well as in the conditioned medium from thyroid follicles in suspension culture. A double band with 16,500 and 15,500 D was detected on sodium dodecyl sulfate polyacrylamide gel electrophoresis. In dot blot and western blot the isolated peptide was immunoreactive with a specific anti-bovine bFGF antibody. For further biochemical characterization, bFGF was isolated from entire porcine thyroid glands by ammonium sulfate precipitation, cation exchange chromatography and heparin affinity chromatography. The material obtained from all three origins was identical concerning affinity to heparin and immunoreactivity with the specific anti-bovine bFGF antibody and induced neovascularization in the chorioallantois membranes of chick embryos. Amino acid sequence analysis of the 16-amino-terminal amino acids of the isolated bFGF was in accordance with the established complete 146-amino-acid bFGF molecule except that glycine in position 10 is replaced by phenylalanine. An additionally identified minor peptide presumably is an amino-terminal-truncated form of bFGF, missing the first 15 amino acids. We conclude that the physiological significance of bFGF released by thyroid cells may be the regulation of angiogenesis during thyroid development and goiter growth.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 5","pages":"458-66"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290458","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18516404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Differences in serum insulin-like growth factor I and sex-hormone-binding globulin levels between late and early prepuberty in patients with idiopathic and organic growth hormone deficiency. 特发性和器质性生长激素缺乏症患者青春期前晚期和早期血清胰岛素样生长因子I和性激素结合球蛋白水平的差异

Acta endocrinologica Pub Date : 1993-11-01 DOI: 10.1530/acta.0.1290419

M Ciaccio, A Belgorosky, M A Rivarola

{"title":"Differences in serum insulin-like growth factor I and sex-hormone-binding globulin levels between late and early prepuberty in patients with idiopathic and organic growth hormone deficiency.","authors":"M Ciaccio, A Belgorosky, M A Rivarola","doi":"10.1530/acta.0.1290419","DOIUrl":"https://doi.org/10.1530/acta.0.1290419","url":null,"abstract":"It has been reported that growth hormone modulates serum levels of insulin-like growth factor I (IGF-I) positively and serum levels of sex-hormone-binding globulin (SHBG) negatively. We have measured IGF-I and SHBG concentrations in 24 prepubertal patients with growth hormone deficiency. Twelve of these patients had had intracranial tumors removed (organic growth hormone deficiency) and 12 had growth hormone deficiency of unknown etiology (idiopathic). Fifty-two prepubertal control subjects, admitted for elective surgery, were also studied. All prepubertal patients were divided into two age groups: older and younger than 7 years of age. In both groups there were patients with multiple pituitary deficiencies on hydrocortisone and/or levothyroxine sodium replacement therapy. In the age group younger than 7 years, serum IGF-I was not significantly different between organic and idiopathic growth hormone deficiency (0.28 +/- 0.21 versus 0.062 +/- 0.071 mU/l) but serum SHBG levels were different (74.6 +/- 33.5 versus 173 +/- 59 nmol/l, p < 0.05). These values were not significantly different from controls (0.47 +/- 0.25 mU/l and 132 +/- 47 nmol/l, respectively). In the age group older than 7 years, there was no significant difference between organic and idiopathic growth hormone deficiency in serum IGF-I (0.33 +/- 0.17 versus 0.16 +/- 0.11 mU/l) or serum SHBG (113 +/- 72 versus 108 +/- 17 nmol/l). These values were significantly different from controls (1.04 +/- 0.36 mU/l and 68.7 +/- 31.7 nmol/l, p < 0.05, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 5","pages":"419-23"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290419","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19267224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effects of 2-buten-4-olide, an endogenous feeding suppressant, on the pulsatile secretion of luteinizing hormone in ovariectomized rats. 内源性喂养抑制剂2-丁烯-4-内酯对去卵巢大鼠促黄体生成素脉动分泌的影响。

Acta endocrinologica Pub Date : 1993-11-01 DOI: 10.1530/acta.0.1290467

S Saito, K Shitsukawa, M Irahara, T Matsuzaki, T Aono

{"title":"Effects of 2-buten-4-olide, an endogenous feeding suppressant, on the pulsatile secretion of luteinizing hormone in ovariectomized rats.","authors":"S Saito, K Shitsukawa, M Irahara, T Matsuzaki, T Aono","doi":"10.1530/acta.0.1290467","DOIUrl":"https://doi.org/10.1530/acta.0.1290467","url":null,"abstract":"The effects of 2-buten-4-olide (2-B40), an endogenous feeding suppressant, on the secretion of luteinizing hormone (LH) were studied in ovariectomized rats. Intraperitoneal (ip) administration of 2-B40: adult female ovariectomized Wistar rats were given daily ip injections of solution containing 2-B40 at 0, 50 or 100 mg/kg body wt for 14 days. This ip treatment with 2-B40 significantly decreased the mean LH concentration and pulse frequency of LH. Intravenous (iv) administration of 2-B40: a solution of 2-B40 (50 or 100 mg/kg body wt) was slowly injected through an intra-atrium catheter and blood samples were taken every 6 min for 2 h. This iv treatment significantly suppressed the LH pulse frequency but had no significant effect on the LH amplitude or mean LH. Injection of 2-B40 into the third cerebroventricle: the injection of 2-B40 into the third cerebroventricle of freely moving rats decreased the mean LH concentration and the frequency and amplitude of LH pulses. Third cerebroventricle injection of a corticotropin-releasing factor (CRF) receptor antagonist before third cerebroventricle injection of 2-B40: the specific CRF receptor antagonist alpha-helical-CRF (9-41) was injected into the third cerebroventricle of ovariectomized rats before injection of 2-B40. Administration of 2-B40 into the third cerebroventricle significantly decreased the mean LH, concentration and pulse frequency. Third cerebroventricle injection of the CRF antagonist at 50 micrograms/rat and then 2-B40 also resulted in significant suppression of the mean LH concentration and pulse frequency.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 5","pages":"467-72"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290467","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19268487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Results of ultrasonically guided percutaneous ethanol injection into parathyroid adenomas in primary hyperparathyroidism. 超声引导下经皮乙醇注射治疗原发性甲状旁腺功能亢进的效果。

Acta endocrinologica Pub Date : 1993-11-01 DOI: 10.1530/acta.0.1290381

B L Vergès, J P Cercueil, D Jacob, G Vaillant, J M Brun, R Putelat

{"title":"Results of ultrasonically guided percutaneous ethanol injection into parathyroid adenomas in primary hyperparathyroidism.","authors":"B L Vergès, J P Cercueil, D Jacob, G Vaillant, J M Brun, R Putelat","doi":"10.1530/acta.0.1290381","DOIUrl":"https://doi.org/10.1530/acta.0.1290381","url":null,"abstract":"Surgery is the usual treatment for primary hyperparathyroidism. However, some patients with high surgical risks are not suitable for surgery. For such patients, we propose, as an alternative treatment, ultrasonically guided percutaneous ethanol injection into parathyroid adenomas, in order to induce necrosis of the tumor. We report, here, the results of ultrasonically guided percutaneous ethanol injection into parathyroid adenomas, during a prolonged follow-up period up to 49 months, in a group of 13 patients (median age 79 years) with primary hyperparathyroidism and contraindications for surgery. In seven patients, complete normalization of plasma calcium, phosphorus and parathyroid hormone (PTH) levels was achieved after ethanol injections, with no recurrence of hypercalcemia during a median follow-up period of 28 months (total success). In these seven patients, plasma calcium, phosphorus and PTH levels were normalized 48 h after the successful ethanol injection. In four patients, a partial success was obtained with clinical improvement and normalization of plasma calcium levels but without complete normalization of plasma PTH levels. This partial success is due to incomplete necrosis of the adenoma, as has been confirmed in one patient by histopathological examination. The ethanol injection treatment failed in only two patients. This treatment was always well tolerated and no major side-effects were observed. In conclusion, our results give evidence that ultrasonically guided percutaneous ethanol injection into parathyroid adenomas can be a very useful alternative therapy in patients not suitable for surgery.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 5","pages":"381-7"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290381","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19267220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Light deprivation increases plasma levels of melatonin during the first 72 h of life in human infants. 在人类婴儿生命的最初72小时内，光剥夺会增加血浆中褪黑激素的水平。

Acta endocrinologica Pub Date : 1993-11-01 DOI: 10.1530/acta.0.1290442

F Jaldo-Alba, A Muñóz-Hoyos, A Molina-Carballo, J A Molina-Font, D Acuña-Castroviejo

{"title":"Light deprivation increases plasma levels of melatonin during the first 72 h of life in human infants.","authors":"F Jaldo-Alba, A Muñóz-Hoyos, A Molina-Carballo, J A Molina-Font, D Acuña-Castroviejo","doi":"10.1530/acta.0.1290442","DOIUrl":"https://doi.org/10.1530/acta.0.1290442","url":null,"abstract":"The development of rhythmic melatonin secretion in full-term neonates seems to occur at about 12 weeks of age, but activity of the pineal gland from 1 to 12 weeks of age is not well documented. To determine whether the pineal gland actively secretes melatonin and reacts to photoperiodic information during this period, we analyzed 45 full-term infants exposed to continuous artificial light during 24, 48 and 72 h after birth for treatment of hyperbilirubinemia. During this light treatment, the eyes of the neonates were completely covered to avoid damage, thus the infants were under continuous light deprivation. Phototherapy significantly decreased plasma bilirubin during treatment. With regard to pineal gland activity, the shortest period of light deprivation tested, 24 h, significantly increased plasma melatonin levels from 152.66 +/- 11.57 to 244.86 +/- 19.49 ng/l (mean +/- SEM; p < 0.001). The other periods tested, 48 and 72 h of light deprivation, led to similar percentages of melatonin stimulation. These results suggest that the pineal gland of neonates, before displaying rhythmic metabolic activity, is sensitive to changes in environmental illumination, indicating maturity of some features of suprachiasmatic nuclei function.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 5","pages":"442-5"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290442","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19268485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42

Somatostatin withdrawal alone is an ineffective generator of pulsatile growth hormone release in man. 单独的生长抑素戒断是男性搏动性生长激素释放的无效发生器。

Acta endocrinologica Pub Date : 1993-11-01 DOI: 10.1530/acta.0.1290414

P J Ho, G B Kletter, N J Hopwood, R DeMott Friberg, A L Barkan

{"title":"Somatostatin withdrawal alone is an ineffective generator of pulsatile growth hormone release in man.","authors":"P J Ho, G B Kletter, N J Hopwood, R DeMott Friberg, A L Barkan","doi":"10.1530/acta.0.1290414","DOIUrl":"https://doi.org/10.1530/acta.0.1290414","url":null,"abstract":"To assess the relative roles of growth hormone-releasing hormone (GHRH) pulse and somatostatin withdrawal as potential generators of pulsatile growth hormone (GH) release in humans, we studied GH responses to iv bolus GHRH (1 microgram/kg) and to termination of a 4 h iv somatostatin infusion (7.2 micrograms.kg-1.h-1) in five normal young men, and in five men with previously diagnosed isolated GH deficiency. The patients were diagnosed 8-15 years previously on the basis of typical auxological and hormonal criteria, were treated with exogenous GH and were off GH therapy for 1.5-8.9 years prior to this study. Growth hormone rises to a bolus GHRH were similar between the controls and the patients (maximum GH 27.3 +/- 15.3 vs 8.0 +/- 4.0 micrograms/l). The controls exhibited only a small GH rise to somatostatin withdrawal (maximum GH 2.9 +/- 1.2 micrograms/l), while the patients did not (maximum GH 0.7 +/- 0.1 micrograms/l; p < 0.05). We conclude that somatostatin withdrawal by itself is an ineffective promoter of GH pulsatility. Periodic quiescence of somatostatinergic neurons must be associated with a concomitant GHRH pulse in order to result in a robust GH pulse.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 5","pages":"414-8"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290414","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18904337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Acute change in parathyroid function in primary hyperparathyroidism following ultrasonically guided ethanol injection into solitary parathyroid adenomas. 超声引导乙醇注射单发甲状旁腺腺瘤后原发性甲状旁腺功能亢进的急性改变。

Acta endocrinologica Pub Date : 1993-11-01 DOI: 10.1530/acta.0.1290377

S Karstrup, L Hegedüs, H H Holm

{"title":"Acute change in parathyroid function in primary hyperparathyroidism following ultrasonically guided ethanol injection into solitary parathyroid adenomas.","authors":"S Karstrup, L Hegedüs, H H Holm","doi":"10.1530/acta.0.1290377","DOIUrl":"https://doi.org/10.1530/acta.0.1290377","url":null,"abstract":"Ultrasonically guided percutaneous injection of 96% ethanol into solitary parathyroid tumours in patients with primary hyperparathyroidism may be used as an alternative to surgery in selected patients. Contrary to surgical parathyroidectomy, the acute changes in parathyroid function following ultrasound-guided chemical parathyroidectomy have never been described. Seven consecutive and highly selected patients with primary hyperparathyroidism were treated with ultrasonically guided injection of ethanol (96%) into solitary and biopsy-verified parathyroid tumours. Basic treatment included a maximum of three injections separated by intervals of 24 h. In six of the seven patients normal serum values of ionized calcium were achieved within 36-120 h (median 36 h) and normal serum values of intact parathyroid hormone within 6-78 h (median 24 h). Three patients received two injections and three patients three injections. One patient remained hypercalcaemic in spite of three injections. Subsequent surgery showed the patient to have two parathyroid adenomas, of which only one had been detected ultrasonically. The present study has demonstrate a fast normalization of parathyroid function following two to three ethanol injections into solitary parathyroid tumors in selected patients with primary hyperparathyroidism.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 5","pages":"377-80"},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290377","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19267219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Analysis of the Gs alpha gene in growth hormone-secreting pituitary adenomas by the polymerase chain reaction-direct sequencing method using paraffin-embedded tissues. 石蜡包埋组织聚合酶链反应-直接测序法分析垂体生长激素腺瘤中Gs α基因。

Acta endocrinologica Pub Date : 1993-10-01 DOI: 10.1530/acta.0.1290301

E Hosoi, Y Yokogoshi, E Hosoi, H Horie, T Sano, S Yamada, S Saito

{"title":"Analysis of the Gs alpha gene in growth hormone-secreting pituitary adenomas by the polymerase chain reaction-direct sequencing method using paraffin-embedded tissues.","authors":"E Hosoi, Y Yokogoshi, E Hosoi, H Horie, T Sano, S Yamada, S Saito","doi":"10.1530/acta.0.1290301","DOIUrl":"https://doi.org/10.1530/acta.0.1290301","url":null,"abstract":"We investigated the prevalence of Gs alpha gene mutations in growth hormone (GH) secreting pituitary adenomas from Japanese patients with acromegaly. Forty-five GH-secreting adenomas were examined for the presence of point mutations in codons 201 or 227 of the Gs alpha gene using the polymerase chain reaction-direct sequencing method and deoxyribonucleic acid extracted from paraffin-embedded tumor specimens. Mutation of codon 227 of the Gs alpha gene was not observed in any of the tumors, but a mis-sense mutation of codon 201 was identified in two tumors (4.4%). One lesion was a densely granulated GH cell adenoma in a patient with adenomatous goiter and breast cancer. The other was a mixed GH cell-prolactin cell adenoma in a patient with multiple endocrine neoplasia type 1 associated with parathyroid hyperplasia and a pancreatic islet cell tumor. The Gs alpha gene detected in parathyroid tissue and pancreatic tumor tissue was of the wild type in this second patient, and the mutation was specific to the pituitary tumor. These results suggest that point mutations of codons 201 or 227 of the Gs alpha gene may not be important mediators of oncogenesis for GH-secreting pituitary adenomas in Japan.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 4","pages":"301-6"},"PeriodicalIF":0.0,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290301","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19225830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46