动物疾病(英文)Pub Date : 2022-01-01Epub Date: 2022-11-03DOI: 10.1186/s44149-022-00053-9
Doaa Higazy, Xianwu Lin, Tanghui Xie, Ke Wang, Xiaochen Gao, Min Cui
{"title":"Altered gene expression in human brain microvascular endothelial cells in response to the infection of influenza H1N1 virus.","authors":"Doaa Higazy, Xianwu Lin, Tanghui Xie, Ke Wang, Xiaochen Gao, Min Cui","doi":"10.1186/s44149-022-00053-9","DOIUrl":"https://doi.org/10.1186/s44149-022-00053-9","url":null,"abstract":"<p><p>Influenza viruses not only cause respiratory illness, but also have been reported to elicit neurological manifestations following acute viral infection. The central nervous system (CNS) has a specific defense mechanism against pathogens structured by cerebral microvasculature lined with brain endothelial cells to form the blood-brain barrier (BBB). To investigate the response of human brain microvascular endothelial cells (hBMECs) to the Influenza A virus (IAV), we inoculated the cells with the A/WSN/33 (H1N1) virus. We then conducted an RNAseq experiment to determine the changes in gene expression levels and the activated disease pathways following infection. The analysis revealed an effective activation of the innate immune defense by inducing the pattern recognition receptors (PRRs). Along with the production of proinflammatory cytokines, we detected an upregulation of interferons and interferon-stimulated genes, such as IFN-β/λ, ISG15, CXCL11, CXCL3 and IL-6, etc. Moreover, infected hBMECs exhibited a disruption in the cytoskeletal structure both on the transcriptomic and cytological levels. The RNAseq analysis showed different pathways and candidate genes associated with the neuroactive ligand-receptor interaction, neuroinflammation, and neurodegenerative diseases, together with a predicted activation of the neuroglia. Likewise, some genes linked with the mitochondrial structure and function displayed a significantly altered expression. En masse, this data supports that hBMECs could be infected by the IAV, which induces the innate and inflammatory immune response. The results suggest that the influenza virus infection could potentially induce a subsequent aggravation of neurological disorders.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1186/s44149-022-00053-9.</p>","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40453144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
动物疾病(英文)Pub Date : 2022-01-01DOI: 10.1186/s44149-022-00051-x
Bianca L Artiaga, Igor Morozov, Russell Ransburgh, Taeyong Kwon, Velmurugan Balaraman, Sabarish V Indran, Darling Melany De Carvalho Madrid, Weihong Gu, Jamie Henningson, Wenjun Ma, Jürgen A Richt, John P Driver
{"title":"Evaluating α-galactosylceramide as an adjuvant for live attenuated influenza vaccines in pigs.","authors":"Bianca L Artiaga, Igor Morozov, Russell Ransburgh, Taeyong Kwon, Velmurugan Balaraman, Sabarish V Indran, Darling Melany De Carvalho Madrid, Weihong Gu, Jamie Henningson, Wenjun Ma, Jürgen A Richt, John P Driver","doi":"10.1186/s44149-022-00051-x","DOIUrl":"https://doi.org/10.1186/s44149-022-00051-x","url":null,"abstract":"<p><p>Natural killer T (NKT) cells activated with the glycolipid ligand α-galactosylceramide (α-GalCer) stimulate a wide variety of immune cells that enhance vaccine-mediated immune responses. Several studies have used this approach to adjuvant inactivated and subunit influenza A virus (IAV) vaccines, including to enhance cross-protective influenza immunity. However, less is known about whether α-GalCer can enhance live attenuated influenza virus (LAIV) vaccines, which usually induce superior heterologous and heterosubtypic immunity compared to non-replicating influenza vaccines. The current study used the swine influenza challenge model to assess whether α-GalCer can enhance cross-protective immune responses elicited by a recombinant H3N2 LAIV vaccine (TX98ΔNS1) encoding a truncated NS1 protein. In one study, weaning pigs were administered the H3N2 TX98ΔNS1 LAIV vaccine with 0, 10, 50, and 100 μg/kg doses of α-GalCer, and subsequently challenged with a heterologous H3N2 virus. All treatment groups were protected from infection. However, the addition of α-GalCer appeared to suppress nasal shedding of the LAIV vaccine. In another experiment, pigs vaccinated with the H3N2 LAIV, with or without 50 μg/kg of α-GalCer, were challenged with the heterosubtypic pandemic H1N1 virus. Pigs vaccinated with the LAIV alone generated cross-reactive humoral and cellular responses which blocked virus replication in the airways, and significantly decreased virus shedding. On the other hand, combining the vaccine with α-GalCer reduced cross-protective cellular and antibody responses, and resulted in higher virus titers in respiratory tissues. These findings suggest that: (i) high doses of α-GalCer impair the replication and nasal shedding of the LAIV vaccine; and (ii) α-GalCer might interfere with heterosubtypic cross-protective immune responses. This research raise concerns that should be considered before trying to use NKT cell agonists as a possible adjuvant approach for LAIV vaccines.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1186/s44149-022-00051-x.</p>","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10717550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
动物疾病(英文)Pub Date : 2022-01-01DOI: 10.1186/s44149-022-00061-9
Lily Tran, Vignesh A Rathinasamy, Travis Beddoe
{"title":"Development of a loop-mediated isothermal amplification assay for detection of <i>Austropeplea tomentosa</i> from environmental water samples.","authors":"Lily Tran, Vignesh A Rathinasamy, Travis Beddoe","doi":"10.1186/s44149-022-00061-9","DOIUrl":"https://doi.org/10.1186/s44149-022-00061-9","url":null,"abstract":"<p><p>Lymnaeid snails are key intermediate hosts for the development and survival of <i>Fasciola</i> spp., the causative agent of Fascioliasis which are economically important parasites infecting humans and livestock globally. The current control method for treating Fascioliasis is heavily reliant on anthelmintic drugs, particularly Triclabendazole (TCBZ) which has resulted in drug-resistant parasites and poses significant risk as there are no long-term efficacious alternatives available. Sustainable control measures at the farm level could include both parasite and snail control will play an important role in <i>Fasciola</i> spp. control and reduce the reliance on anthelmintic drugs. Implementation of such sustainable control measures requires effective identification of snails on the property however Lymnaeid snails are small and difficult to physically locate. Snail identification using an environmental DNA approach is a recent approach in which physically locating snails are not required. <i>Austropeplea tomentosa,</i> is the primary intermediate snail host for <i>F. hepatica</i> transmission in South-East Australia and we present an in-field loop-mediated isothermal amplification and water filtering method for the detection of <i>A. tomentosa</i> eDNA from water samples to improve current surveillance methods. This methodology is highly sensitive with a detection limit of 5 × 10<sup>- 6</sup> ng/μL, detected in < 20 minutes, with cumulative sample preparation and amplification time under 1 hour. This proposed workflow could assist in monitoring areas to determine the risk of Fascioliasis infection and implement strategies to manage snail populations to ultimately reduce the risk of infection for humans and livestock.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1186/s44149-022-00061-9.</p>","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9743122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10460801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction to: Inactivated Pseudomonas PE(ΔIII) exotoxin fused to neutralizing epitopes of PEDV S proteins produces a specific immune response in mice","authors":"Leqiang Sun, Yajie Tang, Keji Yan, Huanchun Chen, Huawei Zhang","doi":"10.1186/s44149-021-00030-8","DOIUrl":"https://doi.org/10.1186/s44149-021-00030-8","url":null,"abstract":"","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41971470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
动物疾病(英文)Pub Date : 2021-10-13DOI: 10.1186/s44149-021-00027-3
R. Shi, Lei Hou, Jue Liu
{"title":"Host immune response to infection with porcine circoviruses","authors":"R. Shi, Lei Hou, Jue Liu","doi":"10.1186/s44149-021-00027-3","DOIUrl":"https://doi.org/10.1186/s44149-021-00027-3","url":null,"abstract":"","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49335107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
动物疾病(英文)Pub Date : 2021-09-22DOI: 10.1186/s44149-021-00023-7
H. K. Tiwari, J. Gogoi-Tiwari, I. Robertson
{"title":"Eliminating dog-mediated rabies: challenges and strategies","authors":"H. K. Tiwari, J. Gogoi-Tiwari, I. Robertson","doi":"10.1186/s44149-021-00023-7","DOIUrl":"https://doi.org/10.1186/s44149-021-00023-7","url":null,"abstract":"","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44419069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of a duplex real-time PCR method for the detection of influenza C and D viruses","authors":"Letian Zhang, Meng Lu, Jiaxuan Lu, Ningning Wang, Zhongzhou Pan, Shuo Su","doi":"10.1186/s44149-021-00016-6","DOIUrl":"https://doi.org/10.1186/s44149-021-00016-6","url":null,"abstract":"","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41719096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
动物疾病(英文)Pub Date : 2021-09-14DOI: 10.1186/s44149-022-00058-4
Xiaoqi Yang, Yang Fu, Lianfeng Wu, Antong Li, Luyao Ji, Hao Li, Yuxuan Peng, Jiabin Zhang, Donghai Zhou, Huiping Zhou
{"title":"Correction to: The dopamine receptor D4 regulates the proliferation of pulmonary arteries smooth muscle in broilers by downregulating AT1R","authors":"Xiaoqi Yang, Yang Fu, Lianfeng Wu, Antong Li, Luyao Ji, Hao Li, Yuxuan Peng, Jiabin Zhang, Donghai Zhou, Huiping Zhou","doi":"10.1186/s44149-022-00058-4","DOIUrl":"https://doi.org/10.1186/s44149-022-00058-4","url":null,"abstract":"","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46944210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
动物疾病(英文)Pub Date : 2021-09-13DOI: 10.1186/s44149-021-00018-4
Jizhou Lv, Hui-yu Wang, Xue-qing Han, Lin Mei, Xiangfen Yuan, Yufang Kong, Junhua Deng, Z. Fu, Shaoqiang Wu, Xiangmei Lin
{"title":"Identification of tick-borne pathogens using metagenomic analyses in H. longicornis feeding on humans in downtown Beijing","authors":"Jizhou Lv, Hui-yu Wang, Xue-qing Han, Lin Mei, Xiangfen Yuan, Yufang Kong, Junhua Deng, Z. Fu, Shaoqiang Wu, Xiangmei Lin","doi":"10.1186/s44149-021-00018-4","DOIUrl":"https://doi.org/10.1186/s44149-021-00018-4","url":null,"abstract":"","PeriodicalId":69105,"journal":{"name":"动物疾病(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41836857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}